Article Per Year (5 Year)
p-Index From 2021 - 2026
1.431
P-Index
This Author published in this journals
All Journal Jurnal Kimia (Journal of Chemistry) METAMORFOSA Journal of Biological Sciences Jurnal Veteriner Jurnal Biologi Udayana CHEMISTRY PROGRESS JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA SIMBIOSIS Metamorfosa: Journal of Biological Science Open Access DRIVERset
Dwi Ariani Yulihastuti
Program Studi Biologi, Fakultas Matematika Dan Ilmu Pengetahuan Alam, Universitas Udayana, Kampus Bukit Jimbaran, Badung, Bali
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Environmental Science Immunology & microbiology Veterinary

Published : 18 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 5 Documents
Search
Journal : METAMORFOSA Journal of Biological Sciences

 1 
Minyak Atsiri Daun Kemangi (Ocimum × africanum Lour.) dan Efektivitasnya Sebagai Lotion Antinyamuk terhadap Aedes aegypti (Linnaeus, 1762) Ni Luh Made Yuli Indra Yanti; Ni Luh Arpiwi; Dwi Ariani Yulihastuti
Metamorfosa: Journal of Biological Sciences Vol 7 No 2 (2020)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2020.v07.i02.p14

Abstract

Aedes aegypti L. mosquito is the vector of dengue fever disease. The effort to control the vector is by applying anti-mosquito lotion to the skin. Basil essential oil was formulated into anttimosquito lotions which is practice and easy to be carried anywhere. The use of atimosquito lotion with natural active compound of basil essential oil is one of safe solutions to repelt mosquitos. This study aimed to calculate yield of essential oil from basil leaves, to identify the active compounds, to measure protection ability of the antimosquito lotions, to to anlyse physical properties of the lotions and to analyse perception of panelists toward the lotions. The essential oil was extracted using steam distillation. The active compounds were analysed using GS-MS. Protection ability of the lotion toard Ae. aegypti L. was tested under laboratory condition using hand in cage method. Physical properties of the lotion were measured for organoleptic, pH, viscosity and homogeneity. Results showed that the yield of essential oil from basil leaves was 0,26% ± 0,05 w/w. GCMS test results showed there were 25 active compounds of the oil. The highest protection ability against A aegypti L. was lotion with 5% basil essential oil. Organoleptic test results show a thick texture, yellowish white color, aroma of olive oil and basil and a soft impression on the skin. Results of pH, viscosity and homogeneity tests show that anti-mosquito lotions containing essential oil from basil leaves met the requirements in the Indonesian National Standard. The hedonic test shows that panelists like lotion with 5% basil essential oil. The effect of lotion gives softness, comfort and the absence of sensitivity symptoms after being applied to the skin. Keywords: Aedes aegypti mosquito, dengue fever, basil essential oil, lotion.
Uji Kandungan Escherichia coli dan Total Bakteri pada Daging dan Organ Dalam Sapi di Rumah Potong Hewan (RPH) Kota Denpasar dan Kabupaten Badung Putu Anjarina; Retno Kawuri; Dwi Ariani Yulihastuti
Metamorfosa: Journal of Biological Sciences Vol 5 No 2 (2018)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2018.v05.i02.p06

Abstract

Penelitian ini ditujukan untuk menguji kandungan E. coli dan cemaran total bakteri daging sapi bagian punggung, daging bagian paha, usus, hati, limfa dan paru di Rumah Potong Hewan (RPH) di Kota Denpasar dan kabupaten Badung, Bali. Hasil kandungan E. coli yang diperoleh dari Rumah Potong Hewan (RPH) Pesanggaran pada sampel paha sebesar 70 APM/100g dan RPH Mambal pada sampel usus sebesar 70 APM/100g. Sampel limfa dari kedua RPH memiliki nilai yang paling rendah yaitu 30 APM/100g. Nilai yang telah dijabarkan dapat dikatakan melebihi ambang batas yang diperbolehkan oleh BPOM (2009) yaitu batas maksimum cemaran E. coli 1 x 101 APM/100g. Hasil penelitian total bakteri yang diperoleh dari sampel yang diambil di RPH Pesanggaran pada sampel limfa menunujukkan hasil yang paling besar yaitu 14x104 CFU/g, sedangkan nilai yang terkecil berasal dari sampel usus yaitu 2x104 CFU/g. Hasil total bakteri yang berasal dari RPH Mambal nilai sampel yang terbesar dimiliki oleh sampel limfa sebesar 13x104 CFU/g, sedangkan nilai hasil terkecil dimiliki oleh sampel paha 1x104 CFU/g diperbolehkan BPOM (2009) yaitu 1x104 CFU/g. Kesimpulan dari penelitian ini menunjukkan sampel daging paha, daging punggung, usus, hati, paru dan limfa memiliki nilai total cemaran E. coli dan cemaran bakteri yang melebihi batas standar yang diperbolehkan oleh BPOM Bali.
PENURUNAN FUNGSI HATI TIKUS BETINA (Rattus norvegivus L) YANG DIINJEKSI WHITE VITAMIN C DOSIS TINGGI DALAM JANGKA WAKTU LAMA DITINJAU DARI KADAR SGPT, SGOT SERTA GAMBARAN HISTOLOGI HATI Ni Wayan Sudatri; Iriani Setyawati; Ni Made Suartini; Dwi Ariani Yulihastuti
Metamorfosa: Journal of Biological Sciences Vol 3 No 1 (2016)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2016.v03.i01.p07

Abstract

This study aims to determine the side effects of high-dose injections of vitamin C in a long term against liver histology. This study used a completely randomized design (CRD) with different long treatments of vitamin C injection in various doses of P0 (control), P1 (30 days injection), P2 (50 days injection), P3 (70 days injection) and P4 (90 days injection). Parameters measured were plasma levels of SGPT and SGOT, the percentage of hydropic degeneration,  fatty degeneration, pyknotik nuclei, and necrosis of liver cells, and also inflammatory cell infiltration and sinusoid congestion. SGOT levels showed a significant differences (P=0,009) between the treatment and control group by ANOVA test. The Kruskal Wallis test showed that ALT levels were not significantly different (P=0,86), however, the levels of creatinine showed significant differences (P=0,002). The hydropic degeneration were not significantly different (P=0,054) by Kruskal Wallis test, whereas the fatty degeneration were significantly different (P=0,000) between the treatment and control groups by ANOVA test. Pyknotik nuclei and necrotic cells showed significant differences between the control and treatment with the P value of 0,016 and 0,01 respetively. The injected white vitamin C in high doses for long periods decreased the physiological functions of female mice liver.
Penurunan Kualitas Sperma Tikus (Rattus novergivus) yang Diinjeksi Vitamin C Dosis Tinggi dalam Jangka Waktu Lama Ni Wayan Sudatri; Dwi Ariani Yulihastuti; Ni Made Suartini
Metamorfosa: Journal of Biological Sciences Vol 6 No 1 (2019)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2019.v06.i01.p02

Abstract

Benefits of white vitamin C to increace the immune system is already exis. However, the use of high doses of vitamin C and in the long term can cause side effects.. The objective of this study was to determine the side effects of high-dose injections of vitamin C in the long term on sperm quality of rats. The design used in this study is completely randomized design (CRD) with long treatment injections of high doses of vitamin C are different. ie: P0 (control), P1 (injected 30 days), P2 (injected 50 days ), P3 (injected 70 days) and P4 (injected 90 days). Parameters measured were sperm quality (motility, viability, morphology, number) ,. The results showed that the sperm quality of rats (Rattus novergivus). Sperm quality of male rats (Rattus norvegivus L.) were injected vitamin C in high doses for long periods of time between the control and treatment groups showed significant differences (P = 0.000). The quality of sperm of mice injected high doses of white vitamin C tend to decrease sperm quality indicated by percentage of motility, viability, and morphology of spermatozoa although the concentration of spermatozoa tends to increase. Keywords: white vitamin C, rat, sperm quality
Kuantitas Dan Kualitas DNA Hasil Ekstraksi Dari Bercak Darah Pada Pisau Pasca Paparan Sinar Ultraviolet Dan Matahari Putri Arie Prasetyoningrum; I Ketut Junitha; Dwi Ariani Yulihastuti
Metamorfosa: Journal of Biological Sciences Vol 10 No 1 (2023)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2023.v10.i01.p19

Abstract

Examination of Deoxyribonucleic Acid is the primary identification method in forensic cases. Blood stains found at the scene of a crime are often affected by various environmental factors, one of which is ultraviolet light from the sun. Purpose of this study is to compare the quantity and quality of DNA extracted from blood stains on the blade after exposure to ultraviolet light and the sun for 0, 15 and 30 days. The method used is a factorial design: light source (UVA, indirect sunlight and direct sunlight); and duration of treatment (0, 15 and 30 days). Blood stains are made by dripping a 50?L of human blood on one side of the blade and then given treatment. DNA analysis includes: extraction with Chelex 5%; DNA quantity and quality test with SimpliNano spectrophotometer; total DNA quality test with agarose gel electrophoresis; and PCR. Quantitative data analysis using Univariate test followed by Duncan test. The results showed that the quantity of DNA in samples exposed to UVA and sunlight (directly) increased on the 15th day due to DNA fragmentation while samples exposed to sunlight (indirectly) decreased the quantity of DNA with the duration of treatment. DNA quality in the form of DNA purity produces an extract of DNA that is not pure. The total DNA quality with agarose gel electrophoresis on all samples showed that the longer the treatment (0, 15 and 30 days) resulted in a dimmer or thinner band of DNA luminescence with smears.
Co-Authors Anak Agung Ketut Darmadi Dimung, Marieta Demarilac Ezra Oktavia Ambarita I Ketut Junitha I Komang Angga Kristiawan I Wayan Sudiarta IRIANI SETYAWATI Ivani Dayanara Kadek Aryndha Sukma Prabaswari Kadek Mardika Maulidya Rosa Putri Utama Ni Komang Lisna Putri Ni LUH ARPIWI Ni Luh Made Yuli Indra Yanti Ni Luh Suriani Ni Made Estriana Pranadya Ni Made Rai Suarni Ni Made Rai Suarni Ni Made Suartini Ni Nyoman Dian Anjani Ni Putu Adriani Astiti Ni Putu Diantariani Ni Wayan Sudatri Parwanayoni, Ni Made Susun Putri Arie Prasetyoningrum Putu Anjarina Retno Kawuri Ruth Ellisa Christiani SANG KETUT SUDIRGA SANG KETUT SUDIRGA Wijaya, I Made Saka