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PENURUNAN FUNGSI HATI TIKUS BETINA (Rattus norvegivus L) YANG DIINJEKSI WHITE VITAMIN C DOSIS TINGGI DALAM JANGKA WAKTU LAMA DITINJAU DARI KADAR SGPT, SGOT SERTA GAMBARAN HISTOLOGI HATI Ni Wayan Sudatri; Iriani Setyawati; Ni Made Suartini; Dwi Ariani Yulihastuti
Metamorfosa: Journal of Biological Sciences Vol 3 No 1 (2016)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2016.v03.i01.p07

Abstract

This study aims to determine the side effects of high-dose injections of vitamin C in a long term against liver histology. This study used a completely randomized design (CRD) with different long treatments of vitamin C injection in various doses of P0 (control), P1 (30 days injection), P2 (50 days injection), P3 (70 days injection) and P4 (90 days injection). Parameters measured were plasma levels of SGPT and SGOT, the percentage of hydropic degeneration,  fatty degeneration, pyknotik nuclei, and necrosis of liver cells, and also inflammatory cell infiltration and sinusoid congestion. SGOT levels showed a significant differences (P=0,009) between the treatment and control group by ANOVA test. The Kruskal Wallis test showed that ALT levels were not significantly different (P=0,86), however, the levels of creatinine showed significant differences (P=0,002). The hydropic degeneration were not significantly different (P=0,054) by Kruskal Wallis test, whereas the fatty degeneration were significantly different (P=0,000) between the treatment and control groups by ANOVA test. Pyknotik nuclei and necrotic cells showed significant differences between the control and treatment with the P value of 0,016 and 0,01 respetively. The injected white vitamin C in high doses for long periods decreased the physiological functions of female mice liver.
DIVERSITAS SERANGGA PADA PERKEBUNAN PEPAYA (Carica papaya L.) DI DAERAH SANUR, DENPASAR, BALI Ni Made Suartini; Ni Wayan Sudatri; Ni Luh Watiniasih
Metamorfosa: Journal of Biological Sciences Vol 2 No 2 (2015)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

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Abstract

The role of insects in papaya plants is as pollinators, pests, parasites and predators of other insects. Referring to the role of insects, this research was conducted to determine the insects on an papaya plantations. Traping method using methyl eugenol and ripe papaya fruit as well as the manual method with insect net used in this research. Each method was repeated three times with an interval of sampling is one week. Insect netting done on the same day with traping method. Samples were preserved and then taken to the laboratory for identification. Environmental factors were measured are: temperature, humidity, latitude and altitude. Insects were identified by reference: Borror et al. (1992), CSIRO I & II (1991) and also Peggie and Amir (2006). Five species belonging to the genus Bactrocera found on methyl eugenol traps and five species belonging to the genus Drosophila was found in ripe papaya fruit traps as well as 16 other species found using insect netting around the papaya plant. In addition, there is also the genus Paracoccus on a papaya plant. Bactrocera papayae and genus Paracoccus is an insect pest on papaya plants.Keywords: diversity, insect, papaya plantation, Sanur
Diversitas Gastropoda pada Habitat Persawahan dengan Ketinggian Berbeda Ni Made Suartini; Ni Wayan Sudatri
Metamorfosa: Journal of Biological Sciences Vol 6 No 2 (2019)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2019.v06.i02.p11

Abstract

Gastropods or often called snails, live in various habitats including in rice fields habitat. There are several species of rice field gastropods which are detrimental because they damage the plants but some can be utilized. The presence of rice field gastropods is influenced by environmental factors. Environmental factors are certainly different at different altitudes, so that it might affect the species of Gastropods that exist. This study aims to determine the diversity of Gastropods found on rice fields at different altitude. Gastropod sampling were carried out in several rice fields with different altitudes, that is under 100 m, 100 m to 300 m and above 300 m to 500 m above the sea level. Sampling were done by making a 1mx1m square placed in each corner of the rice field and in the middle of the rice field. In addition to the rice fields, samples were also taken from irrigation channels that irrigated the fields. Gastropods found were six species where Lymnaea rubiginosa and Digoniostoma truncatum were only found in rice fields in each location and Terebia granifera was only found in irrigation channels. Melanoides tuberculata, Melanoides maculata and Pomacea canaliculata were found in rice fields and irrigation channels. Locations I and II have a moderate diversity index (1,360 and 1,408) and location III is low (0,795).
Prevalensi Leucocytozoonosis Dan Plasmodiosis Pada Itik (Anas plathyrynchos) Yang Dipelihara Dalam Skala Rumah Tangga Kadek Indah Kartika Sari; Ni Wayan Sudatri; Ni Made Suartini
Metamorfosa: Journal of Biological Sciences Vol 8 No 1 (2021)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2021.v08.i01.p06

Abstract

Leucocytozoon and Plasmodium are blood parasitic protozoa that can be found in poultry blood including ducks. These protozoa can cause Leucocytozoonosis and Plasmodiosis in poultry. Spread of the disease through different biological vectors. Similium sp. and Culicoides arakawae as vectors of Leucocytozoonosis and vectors of Plasmodiosis are Culex sp., Culiseta sp., and Aedes sp. This study aims to determine the prevalence of Leucocytozoonosis and Plasmodiosis in ducks that are maintained on a household scale. Ducks are taken from four locations (breeders on a household scale) in Besan Village. In each location, 5 ducks were taken so that the total number of ducks used was 20. This research used purposive sampling method. Samples of duck blood were made as blood smear preparations and stained with 10% Giemsa stain solution then observed under a microscope. The parameters observed were the percentage of blood samples infected with Leucocytozoon and Plasmodium and hematological parameters (total leukocyte count, total erythrocyte count, hemoglobin level, and PCV value). The results showed that Leucocytozoon and Plasmodium were not found in duck blood cells. The prevalence of Leucocytozoonosis and Plasmodiosis in ducks was 0%. The average value of duck blood profile through One Way Anova statistical analysis showed no significant difference between ducks from each sampling location (p> 0.05) and the value of duck blood profile for all samples was within the normal range.
Penurunan Kualitas Sperma Tikus (Rattus novergivus) yang Diinjeksi Vitamin C Dosis Tinggi dalam Jangka Waktu Lama Ni Wayan Sudatri; Dwi Ariani Yulihastuti; Ni Made Suartini
Metamorfosa: Journal of Biological Sciences Vol 6 No 1 (2019)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2019.v06.i01.p02

Abstract

Benefits of white vitamin C to increace the immune system is already exis. However, the use of high doses of vitamin C and in the long term can cause side effects.. The objective of this study was to determine the side effects of high-dose injections of vitamin C in the long term on sperm quality of rats. The design used in this study is completely randomized design (CRD) with long treatment injections of high doses of vitamin C are different. ie: P0 (control), P1 (injected 30 days), P2 (injected 50 days ), P3 (injected 70 days) and P4 (injected 90 days). Parameters measured were sperm quality (motility, viability, morphology, number) ,. The results showed that the sperm quality of rats (Rattus novergivus). Sperm quality of male rats (Rattus norvegivus L.) were injected vitamin C in high doses for long periods of time between the control and treatment groups showed significant differences (P = 0.000). The quality of sperm of mice injected high doses of white vitamin C tend to decrease sperm quality indicated by percentage of motility, viability, and morphology of spermatozoa although the concentration of spermatozoa tends to increase. Keywords: white vitamin C, rat, sperm quality
MENINGKATKAN PRODUKSI BUNGA POTONG ANTHURIUM SP MELALUI PEMANFAATAN PUPUK ORGANIK N. L. Suriani; N. M. S. Parwanayoni; N. M. Suartini
Buletin Udayana Mengabdi Vol 15 No 2 (2016): Jurnal Udayana Mengabdi
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat

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Abstract

The aim of this study was to look at the effectiveness of organic fertilizers in increasing the production of anthurium sp flowers. Method of this research a randomized block design with 4 treatments and 6 replications. Starting with the manufacture of organic fertilizers by using chicken dung and waste cut flowers. Then made an application to the anthurium flowers. Parameters measured were plant height, number of leaves, number of tillers / hill, the amount of interest / clump and flower diameter. The results that the treatment significantly different (P <0.05) with the control. This suggests that organic fertilizers can increase production of cut flower anthurium sp, with the highest amount of interest and the interest of the longest diameter contained in the fertilizer treatment of 1.5 tons / ha. Number of tillers / clump and number of leaves contained in the fertilizer treatment of 2 tons / ha, this is due to the treatment, the number of N fertilizer is too high that the increased leaf production. From the results of this study concluded that organic fertilizers can increase production of flower anthurium sp.
AKTIVITAS ANTIMALARIA EKSTRAK METANOL DAUN MURBEI (Morus alba) PADA MENCIT TERINFEKSI Plasmodium berghei Putu Aurora Vanadis; Ni Made Suartini; Ni Putu Ariantari
Jurnal Farmasi Udayana Vol. 2, No. 1, Tahun 2013
Publisher : Departement of Pharmacy, Faculty of Mathematics and Natural Science, Udayana University

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Abstract

Malaria adalah penyakit infeksi yang disebabkan oleh parasit Plasmodium. Masalah yang muncul dalam pengendalian malaria saat ini adalah resistensi parasit terhadap obat antimalaria yang tersedia. Eksplorasi tanaman obat merupakan salah satu strategi penting dalam penemuan obat antimalaria. Morus alba dari suku Moraceae, telah digunakan dalam sistem pengobatan tradisional Indonesia untuk obat demam dan malaria. Tujuan dari penelitian ini adalah untuk mengevaluasi aktivitas antimalaria ekstrak metanol daun M. alba secara in vivo terhadap mencit yang terinfeksi Plasmodium berghei. Uji aktivitas antimalaria dilakukan dengan metode Peter’s Test. Artesunat digunakan sebagai obat standar dan ekstrak diberikan secara oral sekali sehari pada mencit yang terinfeksi P. berghei selama 4 hari. Persentase parasitemia diamati selama tujuh hari dengan membuat hapusan darah yang diwarnai dengan Giemsa. Data dianalisis menggunakan ANOVA satu arah, Tukey dan probit. Hasil dari penelitian ini menunjukkan bahwa ekstrak metanol daun M. alba memiliki aktivitas antimalaria terhadap mencit terinfeksi P. berghei dengan nilai ED50 dari 12,86 mg/kgBB.
Kualitas Spermatozoa Mencit yang Terpapar Radiasi Sinar-X Secara Berulang (SPERMATOZOA QUALITY OF MICE EXPOSED TO X-RAYS RADIATION IN REPEATED) Ni Wayan Sudatri; Ni Made Suartini; Anak Agung Sagung Alit Sukmaningsih; Dwi Ariani Yulihastuti
Jurnal Veteriner Vol 16 No 1 (2015)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

In radiology, X-ray has been used to diagnose disease and therapy. However, behind the technologybenefits provided by the radiation, the negative effects are often debated. The purpose of this study was toinvestigate the effects of repeated radiation on sperm quality mice (Mus musculus L). Thirty- two adultmale mice aged three months were divided into groups P1 (1x 200 rad), P2 (2x200 rad), P3 (3x200 rad) andcontrol irradiated with x-rays according to the experimental design . Spermatozoa quality parametersobserved were : number of spermatozoa, motility, viability and morphology of spermatozoa. The results ofthe Post Hoc LSD tests for significant differences (P>0.05) between the control and treatment showed thatthe X-ray radiation exposure to 1x200 rad, 2x200 rad, and 3x200 rad decreases the motility, viability,normal morphology and number spermatozoa produced compared with controls. This is caused by exposureto X-ray radiation causes the formation of free radicals in the body that damage sperm cells mice. Exposureto X-ray radiation repeatedly lowered the quality of spermatozoa of mice.
DIVERSITAS SERANGGA PERMUKAAN TANAH PADA PERTANIAN HORTIKULTURA ORGANIK DI BANJAR TITIGALAR, DESA BANGLI, KECAMATAN BATURITI, KABUPATEN TABANAN-BALI Samsul Ma'arif; Ni Made Suartini; I Ketut Ginantra
Jurnal Biologi Udayana Vol 18 No 1 (2014): Jurnal Biologi
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

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Abstract

Penelitian ini bertujuan untuk mengetahui diversitas serangga permukaan tanah pada pertanian hortikultura organik di Banjar Titigalar, Desa Bangli, Kecamatan Baturiti, Kabupaten Tabanan-Bali. Penelitian dilaksanakan dari bulan Oktober-Desember 2013. Sampel diambil menggunakan Pitfall Trap. Analisa data dilakukan dengan metode deskriptif yaitu dengan mendeskripsikan serangga yang ditemukan dan kemudian diidentifikasi sampai ke tingkat Genus. Hasil penelitian menunjukkan, terdapat 3.066 individu serangga yang termasuk ke dalam 20 genus, 17 famili dan 7 ordo.  Keseluruhan Ordo serangga yang ditemukan adalah Hymenoptera, Orthoptera, Collembola, Coleoptera, Hemiptera, Homoptera, dan Diptera. Diversitas serangga tertinggi ditemukan pada bulan Oktober dengan nilai Indeks Diversitas (H’)=1,5821 pada malam hari, dan H’=1,3978 pada siang hari, dibandingkan yang paling rendah terjadi pada bulan Desember dengan indseks diversitas H’= 0,5829 pada malam hari dan H’= 0,9223 pada siang hari.
STRUKTUR DAN PRODUKSI LEBAH Trigona spp. PADA SARANG BERBENTUK TABUNG DAN BOLA Putu Ade Hinduari Putra; Ni Luh Watiniasih; Ni Made Suartini
Jurnal Biologi Udayana Vol 18 No 2 (2014): JURNAL BIOLOGI
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

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Abstract

Lebah tanpa sengat (Trigona spp.) dapat menghasilkan madu yang bermanfaat bagi kesehatan. Penelitian bertujuan untuk mengetahui : (1) morfologi Trigona spp., (2) struktur internal sarang Trigona spp. pada sarang berbentuk tabung dan bola, (3) volume sarang serta perkiraan produksi madu, beebread dan selanakan Trigona spp. pada sarang berbentuk tabung dan bola. Penelitian dilaksanakan pada bulan November 2014. Sampel yang digunakan adalah koloni dan sarang Trigona spp. berbentuk tabung dan bola yang diambil di Desa Padang Tunggal,Kecamatan Selat, Kabupaten Karangasem, Bali. Hasil penelitian menunjukan bahwa nama spesies dari sampel koloni Trigona spp. pada sarang berbentuk tabung dan bola adalah Trigona laeviceps. Struktur internal sarang Trigona spp. pada sarang berbentuk tabung dan bola mempunyai pola susunan yaitu pot madu, pot beebread dan pot sel anakan. Volume sarang besar memberikan perkiraan total jumlah produksi madu, beebread dan selanakan lebih banyak dibandingkan volume sarang kecil.