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All Journal International Journal of Biosciences and Biotechnology METAMORFOSA Journal of Biological Sciences Jurnal Biologi Udayana E-Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Caraka Tani: Journal of Sustainable Agriculture Advances in Tropical Biodiversity and Environmental Sciences
I Gede Ketut Susrama
Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Environmental Science Immunology & microbiology Veterinary

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Uji Aktivitas Antimikroba Beberapa Ekstrak Bumbu Dapur terhadap Pertumbuhan Jamur Curvularia lunata (Wakk.) Boed. dan Aspergillus flavus LINK. IRMA SELVYANA Br. SITEPU; I KETUT SUADA; I GEDE KETUT SUSRAMA
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol. 1, No. 2, Oktober 2012
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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ABSTRACT The Antimicrobial Activity Test of Some Kitchen Seasoning Extracts on Growth of Fungus Curvularia lunata (Wakk.) Boed. and Aspergillus flavus LINK. This research was aimed to determine the ability of herbs extract in inhibiting the growth of fungus C. lunata and A. flavus and to determine the minimum inhibition concentration of each extracts as well. The results showed that the extracts could inhibit the growth of the two fungus. The most effective extract was turmeric against C. lunata and galangal extract against A. flavus with each inhibition was 38,6% and 26,6% respectively. The minimum inhibition concentration of all extracts were 0,5% on both C. lunata and A. flavus. Keyword : Antimicrobial, Kitchen seasoning, Curvularia lunata, Aspergillus flavus
Identifikasi Mikoriza Vesikular Arbuskular (MVA) dari Rhizosfer Tanaman Cabai (Capsicum annuum L.) dan Tomat (Solanum lycopersicum L.) serta Perbanyakannya Menggunakan Media Zeolit I WAYAN EKA ADI WIRAWAN; I KETUT SUADA; I GEDE KETUT SUSRAMA
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.4, No.4, Oktober 2015
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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ABSTRACT Vesicular Arbuscular Mycorrhizae (VAM) Identification of Chilli (Capsicum annuum L.) and Tomato (Solanum lycopersicum L.) Rhizosphere and Its Spore Multiplication in Zeolite Media Various attempts have been made to increase the production of chilli and tomato plants by farmers, including by using  inorganic fertilizer application on and on going basis. Considering potential problems that may occured due to inorganic fertilizer use, aplication of  biological fertilizer which one of them is containing vesicular arbuscular mycorrhizae (VAM) could be expected to assist the growth of chilli and tomato in more naturally manner. This study was aimed to determine genus and species of VAM in chilli and tomato rhizosphere, its colonization in root tissue, and to examine zeolite media compatibility with corn as a symbiont. Based on the results of the study, it was found four species namely Acaulospora fofeata, A. colombiana, A. Laevis, and Glomus ambisporum in chilli and four species in tomato that were identified as A. fofeata, A. colombiana, Scutellospora calospora, and G. ambisporum. Colonization were found in roots of chilli, tomato, and corn indicated by symbiotic structures arbuscules, vesicles, and inner spores. Zeolite media with corn as symbiotic plant is considered suitable for VAM spore propagation. Keywords: rhizosphere, Acaulospora fofeata, zeolite, inner spore
Kebutuhan Nutrisi dan Substansi dalam Pakan Buatan Serangga (Artikel Ulasan) I GEDE KETUT SUSRAMA
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.6, No.3, Juli 2017
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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Insect Nutritional Requirements and Substances in its Artificial Diet In its life, insect as like as other creatures on earth need nutrients for the metabolism in their body so that the larval stage can grow to adult insect and then can do copulation to produce fertile eggs for its life cycle and for the existence sustainability of their species. Nutrional requirements among insects are certainly not the same depending on insect classification and actually different from species to species however in general there are major nutritional requirements that must available in an insect artificial diet. The nutrional requirements of insects and substances that commonly available in an artificial diet composition for insect are discussed in this article review.
Isolasi dan Identifikasi Agrobacterium tumefaciens dari Tanaman Wortel (Daucus carota L.) YOLANDA HASSIAN MANALU; I GEDE PUTU WIRAWAN; I GEDE KETUT SUSRAMA
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.3, No.3, Juli 2014
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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ABSTRACT Isolation and Identification of Agrobacterium tumefaciens from  Carrots (Daucus carota L.) Agrobacterium tumefaciens is a plant pathogenic bacterium that is widely used as a vector for inserting foreign genes into a plant chromosome to produce a transgenic plant. This bacterium cause a disease namely crown gall in dicotyledonous plants. This study is aim to isolate A. tumefaciens from local carrot (Daucus carota L.) by using some method such as selection media, characterization of the bacteria, and confirm by Koch’s postulates. The result of this study determined that carrot’s root taken from Bedugul area which showed crown gall symptom, an A. tumefaciens due to its characteristics e.g. shape of colonies, color, and growth of the bacteria in AB minimal medium that was a specific for A. tumefaciens.  By using the Koch’s postulates test showed that the isolated caused convex gall on the surface of carrot slice. DNA of isolated bacterium successfully electroforeted through agarose gel electrophoresis.  These results showed that the bacteria associated with carrot was A. tumefaciens. Keywords : A. tumefaciens, AB medium, Carrot, LB medium, Crown gall
Isolasi dan Identifikasi Agrobacterium Tumefaciens pada Tanaman Mawar (Rosa sp.). NADIAH SILITONGA; I GEDE PUTU WIRAWAN; I GEDE KETUT SUSRAMA
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.3, No.3, Juli 2014
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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ABSTRACT Isolation and Identification of Agrobacterium tumefaciens At Plant roses (Rosa sp.). Agrobacterium tumefaciens caused crown gall tumor in many of dicotyledonous plants. The purpose of this study tried to isolate and identify the A. tumefaciens from rose plant. Various techniques were used such as selection media, colonies shape and color, Koch's postulate test, DNA isolation and agarose gel elektroforesis. The results of this study showed that A. tumefaciens can be isolated through culturing in LB medium and selection AB medium that was specific for A. tumefaciens. The isolate caused crown gall tumor on carrot slice 3 weeks after inoculation by using a modified Koch’s postulate test. The characteristics of colony formed in this research are round shaped, cream coloured with pink tint, smooth edge, and convex elevation. DNA isolation and its running in agarose gel electroforesis showed positive result.   Key words : Agrobacterium tumefaciens, AB medium and Agarose gel electroforesis.
Isolasi dan Karakterisasi Cendawan Mikoriza Arbuskular pada Tanah dan Akar Tanaman Jagung di Desa Sanur Kaja I WAYAN PRASTITA DIASTAMA; I GEDE KETUT SUSRAMA; I GEDE PUTU WIRAWAN
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.4, No.1, Januari 2015
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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Abstract

ABSTRACT Isolation and Characterization of Mycorrhizae Arbuskular Fungi in the Soil and Roots of Corn (Zea Mays L.) in the Sanur Kaja Village Mycorrhiza is an organism that is derived from the fungus that describes a form of symbiotic relationship between mutualisme functions with a high degree of plant roots. Benefits of mycorrhiza for plant growth and development as its host is to increase the absorption of nutrient elements of soils, as biological barrier against infection of root pathogen, enhancing the resilience of crops to drought and increasing hormone boosters grows.This research aims to identify a Arbuskular Mycorrhiza fungi isolated from corn plants rhizosphere in the village of Sanur Kaja. The results of this research show that the isolation and characterization of spores that develop on the roots of corn in the village of Sanur Kaja indicates a growing spores there is the genus Glomus sp. type of Spore that successfully identified a species of Glomus sp-1, Glomus sp-2, Glomus sp-3. Key words:Glomus sp, rhizosphere, symbiotic mutualisme
Induced Mutagenesis in Yellow Flowering Marigold with Colchicine in Hydrogen Peroxide I Gede Ketut Susrama; Ketut Ayu Yuliadhi
Advances in Tropical Biodiversity and Environmental Sciences Vol 4 No 2 (2020): ATBES
Publisher : Institute for Research and Community Services Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (493.478 KB) | DOI: 10.24843/ATBES.2020.v04.i02.p04

Abstract

Marigold flower has many benefits in many aspects of life in Bali Island (Indonesia) including often used in religious ceremonial activities. For controlling plant pests and diseases, marigold is planted on the sidelines or on the edge of a land as an attractant crop to invite natural enemies and marigold also can produce certain substance which can reduce the incidence of nematode. In inducing mutagenesis in this research, the seeds of marigold were soaked in solution of colchicine with hydrogen peroxide as its solvent. The results of this research indicated that the treatments caused the formation of chlorophyll mutant and an increase in the length of flower diameter. Both the control and treated plant samples were attacked by leaf miner, giant land snail, virus-like disease and botrytis flower blight disease.
Performance Evaluation of Induced Mutagenesis using Colchicine and EMS Solution on Cowpea M3 Purple and Mung Bean Vima1 to Increase Resistance I Gede Ketut Susrama; Ni Made Trigunasih; I ketut Suada; Ni Nyoman Ari Mayadewi
Caraka Tani: Journal of Sustainable Agriculture Vol 37, No 2 (2022): October
Publisher : Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/carakatani.v37i2.56341

Abstract

Induced mutagenesis is one way to improve the quality of crops, especially to increase the resistance to pests and diseases. This research aimed to determine colchicine and ethyl methane sulfonate (EMS) mutagenesis on cowpea M3 purple and mung bean Vima1, in concatenation for developing resistant crops. This research consists of four packages: (1) first package (cowpea M3 purple seeds treated with colchicine solution, (2) second package (cowpea M3 purple seeds treated with EMS solution), (3) third package (mung bean Vima1 seeds treated with colchicine solution) and (4) fourth package (mung bean Vima1 seeds treated with EMS solution). The results of this research revealed that induced mutagenesis by colchicine solution treatment reduced the incidence of bean leaf beetles up to 19% on cowpea M3 purple, as well leaf miners by 5% to 9% and bean leaf beetles up to 5% on mung bean Vima1. Treatment of EMS solution decreased the incidence of bean leaf beetles by 17% on cowpea M3 purple and pink mealybug by 5% to 15% on mung bean Vima1. Induced mutagenesis using EMS solution significantly decreased vegetable leaf miner incidence by 33% to 93% or 71% on average. Colchicine and EMS solution treatment caused aphid attacks in cowpea M3 purple, particularly in pods. The attacks did not happen on leaves and aphid incidence in pods was slower than in control. Some promising mutant candidates were found from this research that will be used in further crop development studies. It is expected that the method and results of this research could inspire faster development of resistant crops.
Co-Authors D.N. KASNIARI Dewa Ketut Suanda Evy Prima I GEDE PUTU WIRAWAN I Ketut Suada I Made Mega I WAYAN EKA ADI WIRAWAN I WAYAN PRASTITA DIASTAMA IRMA SELVYANA Br. SITEPU KETUT AYU YULIADHI Made Adnyana MADE SRITAMIN NADIAH SILITONGA Ni Kadek Sintya Dewi NI LUH MADE PRADNYAWATHI NI MADE TRIGUNASIH NI NYOMAN ARI MAYADEWI PUTU SENA WIDIATMA ROMAULI THERESIA NAINGGOLAN WAYAN ADIARTAYASA YOLANDA HASSIAN MANALU Yundari, Yundari