Article Per Year (5 Year)
p-Index From 2020 - 2025
1.259
P-Index
This Author published in this journals
All Journal Jurnal Kedokteran Syiah Kuala International Journal of Nasopharyngeal Carcinoma Budapest International Research in Exact Sciences (BirEx Journal) Jurnal Kedokteran Hewan Journal of General-Procedural Dermatology & Venereology Indonesia
Reno Keumalazia Kamarlis
Departement Of Pathology Anatomy, Faculty Of Medicine, University Of Syiah Kuala/Dr. Zainoel Abidin General Hospital, Banda Aceh, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Civil Engineering, Building, Construction & Architecture Health Professions Immunology & microbiology Medicine & Pharmacology Physics Transportation Veterinary

Published : 8 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 2 Documents
Search
Journal : Jurnal Kedokteran Hewan

 1 
GROWTH INHIBITION AND INDUCTION OF APOPTOSIS IN MCF-7 AND T47D BREAST CANCER CELL LINES BY ETHANOL EXTRACT OF SEURAPOH (Chromolaena odorata) LEAVES Hanifah Yusuf; Reno Keumalazia Kamarlis; Yusni Yusni
Jurnal Kedokteran Hewan Vol 14, No 3 (2020): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (641.485 KB) | DOI: 10.21157/j.ked.hewan.v14i3.17227

Abstract

This study aims to determine the growth inhibition and apoptosis induction of MCF-7 and T47D cancer cells by ethanol extract of Chromolaena odorata leaves. A post-test with control group design was used in this study. The extract was made by maceration with 80%ethanol and the tested concentrations used were 7.80 µg/mL-500 µg/mL with standard drug doxorubicin ranged from 1.56 µg/mL-100 µg/mL.The growth inhibition was determined by the MTT colorimetry method, apoptosis induction by double staining using acridine orange-ethidiumbromide, and the existence of apoptosis was proven immunocytochemically through the expression of Bcl-2 proteins. The results showed that the growth inhibition of MCF-7 was 100.29%-28.19% and T47D was 100.37%-16.01%. The IC50 values of MCF-7 was 327.34 µg/mL and T47D was 135.16 µg/mL. The presence of apoptosis was marked by finding the morphological change of cells such as dead, necrosis, and chromatin condensation cells. This suggests that interventions with ethanol extract of Chromolaena odorata leaves can induce apoptosis that has been proven by reducing the expression of Bcl-2 proteins.
GROWTH INHIBITION AND INDUCTION OF APOPTOSIS IN MCF-7 AND T47D BREAST CANCER CELL LINES BY ETHANOL EXTRACT OF SEURAPOH (Chromolaena odorata) LEAVES Yusuf, Hanifah; Kamarlis, Reno Keumalazia; Yusni, Yusni
Jurnal Kedokteran Hewan Vol 14, No 3 (2020): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v14i3.17227

Abstract

This study aims to determine the growth inhibition and apoptosis induction of MCF-7 and T47D cancer cells by ethanol extract of Chromolaena odorata leaves. A post-test with control group design was used in this study. The extract was made by maceration with 80%ethanol and the tested concentrations used were 7.80 g/mL-500 g/mL with standard drug doxorubicin ranged from 1.56 g/mL-100 g/mL.The growth inhibition was determined by the MTT colorimetry method, apoptosis induction by double staining using acridine orange-ethidiumbromide, and the existence of apoptosis was proven immunocytochemically through the expression of Bcl-2 proteins. The results showed that the growth inhibition of MCF-7 was 100.29%-28.19% and T47D was 100.37%-16.01%. The IC50 values of MCF-7 was 327.34 g/mL and T47D was 135.16 g/mL. The presence of apoptosis was marked by finding the morphological change of cells such as dead, necrosis, and chromatin condensation cells. This suggests that interventions with ethanol extract of Chromolaena odorata leaves can induce apoptosis that has been proven by reducing the expression of Bcl-2 proteins.
Co-Authors . Vera Dewi Mulia, Troef Soemarno Benny Kurnia Benny Kurnia Budi Yanti Chesy Oety Otawa Cut Asmaul Husna Earlia, Nanda Fera Kamila Kamal Fitri Dewi Ismida Handriani, Risna Hanifah Yusuf HANIFAH YUSUF Istanul Badiri Kartini Hasballah Maulida, Mimi Pradistha, Aldilla Yusni Yusni Yusni Yusni Yusni Yusni