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Cytotoxicity analysis of alginate impression materials based red seaweed extract on cultured gingival fibroblast cells Praharani, Depi; Barid, Izzata; Indahyani, Didin Erma; Probosari, Niken; Lestari, Sri; Sulistiyani, Sulistiyani; Sumono, Agus
Odonto : Dental Journal Vol 11, No 2 (2024): December 2024
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30659/odj.11.2.290-297

Background: Alginate is an impression material that is widely used in dentistry. Alginate can actually also be obtained from natural materials such as red seaweed. The impression procedure causes the impression material to come into contact with oral tissues including the gingiva. Ideally, the materials used must also meet requirements such as low toxicity or non-toxicity so that tissue damage does not occur. This study aims to analyze the cytotoxicity of alginate impression materials from red seaweed extract in gingival fibroblast cell. Method: This experimental laboratory design using post-test only control group design. The research groups consisted of: sodium alginate extract group, red seaweed extract-based alginate impression material, positive control and negative control. Cytotoxicity was tested on gingival fibroblast cell cultures and the effect was analyzed using the MTT assay. Exposure to gingival fibroblast cell cultures was differentiated in three time durations: 5 minutes, 10 minutes and 15 minutes. Each time duration was repeated three times. MTT-formazan production is a method used to measure cell viability (living cells). The data obtained were statistically analyzed using two-way ANOVA test and Tukey HSD post hoc test. Result: There was no significant difference in the average cell viability between the red seaweed extract-based alginate impression material group and the negative control group at an exposure duration of 5 minutes, which was more than 90%. Conclusion: The red seaweed extract-based alginate impression material has no toxic effect on gingival fibroblast cells at 5 minutes exposure.

Cytotoxicity analysis of alginate impression materials based red seaweed extract on cultured gingival fibroblast cells Praharani, Depi; Barid, Izzata; Indahyani, Didin Erma; Probosari, Niken; Lestari, Sri; Sulistiyani, Sulistiyani; Sumono, Agus
Odonto : Dental Journal Vol 11, No 2 (2024): December 2024
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30659/odj.11.2.290-297

Background: Alginate is an impression material that is widely used in dentistry. Alginate can actually also be obtained from natural materials such as red seaweed. The impression procedure causes the impression material to come into contact with oral tissues including the gingiva. Ideally, the materials used must also meet requirements such as low toxicity or non-toxicity so that tissue damage does not occur. This study aims to analyze the cytotoxicity of alginate impression materials from red seaweed extract in gingival fibroblast cell. Method: This experimental laboratory design using post-test only control group design. The research groups consisted of: sodium alginate extract group, red seaweed extract-based alginate impression material, positive control and negative control. Cytotoxicity was tested on gingival fibroblast cell cultures and the effect was analyzed using the MTT assay. Exposure to gingival fibroblast cell cultures was differentiated in three time durations: 5 minutes, 10 minutes and 15 minutes. Each time duration was repeated three times. MTT-formazan production is a method used to measure cell viability (living cells). The data obtained were statistically analyzed using two-way ANOVA test and Tukey HSD post hoc test. Result: There was no significant difference in the average cell viability between the red seaweed extract-based alginate impression material group and the negative control group at an exposure duration of 5 minutes, which was more than 90%. Conclusion: The red seaweed extract-based alginate impression material has no toxic effect on gingival fibroblast cells at 5 minutes exposure.

Analisa Pengetahuan PHBS pada Kader Posyandu Desa Tamanan Bondowoso dalam Upaya Penurunan Stunting Pada Balita Muhammad Nurul Amin; Tecky Indriana; I Dewa Ayu Ratna Dewanti; Nurud Diniyah; Budi Yuwono; Agus Sumono; Roedy Budirahardjo; Banun Kusumawardani
Dental Agromedis Vol. 2 No. 2 (2024): November
Publisher : Fakultas Kedokteran Gigi, Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/da.v2i2.1722

Stunting in toddlers is related to growth and development disorders. One important factor in preventing stunting is the implementation of Clean and Healthy Living Behaviour (CHLB). CHLB plays a role in improving the nutritional and health status of children, as well as preventing various diseases that can contribute to stunting. The role of cadres is very important because cadres are responsible for implementing the posyandu program, are the main movers in promotive, preventive activities and are motivators for community members. The aim of this service is to analyse knowledge about PHBS among posyandu cadres in Tamanan Village, Tamanan District, Bondowoso Regency. The service method is an outreach activity aimed at providing knowledge about CHLB with the hope that people will change their behaviour to protect their health and the environment independently. Formative evaluation procedures in the form of pretest and posttest. Data were analyzed using descriptive analysis techniques. Continue with the normality test and paired sample t-test. The results obtained were that this community service activity was carried out on all posyandu cadres. Evaluation showed that there was a difference in the average test scores before and after the counselling. Providing counselling is able to provide changes in the knowledge of posyandu cadres which is marked by an increase in posttest scores which are higher compared to posttest scores. The conclusion is that the knowledge of Tamanan Bondowoso Village Posyandu cadres regarding CHLB in efforts to reduce stunting in toddlers can be increased through the counselling provided.Keywords: CHLB, counseling, posyandu cadres, stunting.

THE EFFECTIVENESS OF ANCHOVY INTAKE ON EPITHELIAL SOCKET THICKNESS POST EXTRACTION Indriana, Tecky; Sumono, Agus; Sholihah, Kunti
Odonto : Dental Journal Vol 9, No 1 (2022): July 2022
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Background: Epithelial formation or re-epithelialization is one of the parameters in the wound closure. Nutrition has become a major factors in the successful outcome during this process. Anchovy (Stolephorus sp.) contains several proteins, vitamins, and minerals that can act as supplements to support wound closure. This research proposed to understand the effect of anchovy intake toward epithelial thickness of the rats post tooth extraction.Method: This research was an experimental laboratory with a post-test-only control group design using 24 rats as a sample. Rats were divided into 2 groups, the control and the treatment group with 12 rats each. The mandibular left first molar of the samples was extracted, then given with aquadest (the control) and anchovy powder (the treatment) during 3, 5, and 7 days. All rats were decapitated after 24 hours from the last treatment, followed by tissue processing and staining with Hematoxylin-Eosin (HE) for examination. A microscope that connects with optilab on a magnification of 100x was used to measure the epithelial thickness on the thickest and thinnest part of the epithelial. The data were analysed with One-way ANOVA test and LSD test. Result: The results showed that the epithelial thickness of the treatment group was significantly increased compared to the control group (p<0.05). Conclusion: This study concludes that the anchovy (Stolephorus sp.) intake affects increasing epithelial socket thickness of the rats post tooth extraction.

Beneficial Effect of Arabica Coffee Fruit Skin (Coffea Arabica) on Epithelial Thickness after Tooth Extraction Fadhila Zidni Ilma; Tecky Indriana; Agus Sumono
Denta Journal Kedokteran Gigi Vol 15 No 1 (2021): Februari
Publisher : Fakultas Kedokteran Gigi Universitas Hang Tuah

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30649/denta.v15i1.3

Background: In Indonesia, tooth extraction is the most common practice in dentistry. A successful indicator of tooth extraction is a perfect healing process. One of the parameters of a perfect healing process is epithelial formation. Based on previous research, arabica coffee fruit skin has proven potential to accelerate the wound healing process. The compounds in the arabica coffee fruit skin are mostly composed of flavonoids, tannins, and chlorogenic acid. Purpose: This research is proposed to understand the beneficial effect of arabica coffee fruit skin (Coffea arabica) towards the increase of epithelial thickness in post tooth extraction socket. Material and Method: The type of this research was laboratory experimental in vivo with post-test only control group design using 24 Wistar rats as a sample. The sample was separated into two groups, control and intervention groups with 12 Wistar rats each. Treatment in both groups was given during 3, 5, and 7 days. Afterward, tissue processing undergoes with buccolingual cutting and HE coloring. Measurements are carried out with the ImageRaster software in the thickest and thinnest part of the epithelium that covers the tooth socket. Result: Epithelial thickness after tooth extraction increased significantly (p<0.05) in the intervention group compared to the control group. Conclusion: Arabica coffee fruit skin has the beneficial effect of increasing the epithelial thickness after tooth extraction.

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