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All Journal International Journal of Advances in Applied Sciences Jurnal MIPA CHEMISTRY PROGRESS JURNAL LPPM BIDANG SAINS DAN TEKNOLOGI The Studies of Social Sciences
Maureen Kumaunang
Jurusan Kimia, Fakultas Matematika Dan Ilmu Pengetahuan Alam, Universitas Sam Ratulangi, Manado 95115
Biochemistry, Genetics & Molecular Biology Chemistry Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Environmental Science Materials Science & Nanotechnology Mathematics Physics Social Sciences

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Journal : CHEMISTRY PROGRESS

 1 
KARAKTERISASI PRODUK GEN PENGKODE CHAPERONE BACILLUS SP. RP1 Kumaunang, Maureen; Mandik, Yohanis
CHEMISTRY PROGRESS Vol 3, No 1 (2010)
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35799/cp.3.1.2010.75

Abstract

Molecular chaperone is the group of protein that assist in the correct folding or assembly of other proteins invivo. The objective of this research was to characterize the product of gene encoding chaperone from Bacillussp. RP1 by using in-silico analysis. Analysis of 1053 bp from DNA fragment isolated by PCR showed that it has97% similarity with putative chaperone of Geobacillus kaustophilus (BA000043.1) and 86% similarity with DnaKoperon of B. stearothermophilus (X90709.1). Structure prediction of DnaJ Bacillus sp. RP1 showed that it hassimilar J-domain with that of DnaJ Escherichia coli but cys-rich domain is different.
ANALISIS IN-SILICO PROTEIN DnaJ Bacilus stearothermophilus Kumaunang, Maureen; Kamu, Vanda S.; Mandik, Yohanis I.
CHEMISTRY PROGRESS Vol 2, No 1 (2009)
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35799/cp.2.1.2009.63

Abstract

DnaJ is a chaperone which has function in facilitating folding, translocation, and degradation of protein.The aim of this research was to undertake in silico study of DnaJ protein from Bacillus stearothermophilus.Structure analysis of DnaJ B. stearothermophilus showed that it has J-domain which has two conservedmotifs, i.e. HPD and QKRA motifs. It also has cys-rich domain which has one conserved motif, i.e.CXXCXGXG. Structure prediction of DnaJ B. stearothermophilus showed that it has similar structure of Jdomainand cys-rich domain with that of DnaJ Escherichia coli.
ANALISIS IN-SILICO PROTEIN TIOL-DISULFIDA ISOMERASE FAMILI Bacillus Kumaunang, Maureen
CHEMISTRY PROGRESS Vol 3, No 2 (2010)
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35799/cp.3.2.2010.18982

Abstract

ABSTRACTKumaunang et al., 2010. Characterization of transcript genome product from Chaperone Bacillus sp. RP1.Protein folding is facilitated by chaperone molecule an folding catalyst.The aim of this research was to characterize the gene product of thiol-disulfide oxidoreductase gene from thermophylic organism Bacillus sp. Method used in this research were isolation and purification of deducted gene and characterization of gene product. The result showed that isolated gene has 1.4 kbp in length. Characterization of gene product indicated three proteins, Bdbdred, Bdbdox, and Etda, that have thioredoxin and DsbA motifs, and also active site and bonding site with Zn. Structure prediction of these three proteins showed similarity among them.Keywords : chaperone, thiol-disulfide oxidoreductase, thioredoxin, DsbA, Bdbd
AMOBILISASI ENZIM BROMELIN YANG DIISOLASI DARI BATANG NANAS DENGAN MENGGUNAKAN KARAGENAN Kumaunang, Maureen
CHEMISTRY PROGRESS Vol 4, No 2 (2011)
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35799/cp.4.2.2011.4979

Abstract

Telah dilakukan penelitian untuk menentukan aktivitas enzim bromelin amobil yang diisolasi dari batang nanas.Bromelin adalah enzim yang diisolasi dari nanas yang tergolong kelompok enzim protease sulfhidril. Penelitianini bertujuan untuk: (1) mengamobilisasi enzim bromelin yang diisolasi dari batang buah nanas; (2)menentukan aktivitas enzim bromelin dari batang buah nanas sebelum dan setelah diamobilisasi. Isolasi enzimbromelin dilakukan dengan menggunakan metode pengendapan dengan amonium sulfat. Amobilisasi enzimbromelin dilakukan dengan cara penjebakan menggunakan karagenan. Penentuan aktivitas enzim dilakukandengan menentukan aktivitasnya pada temperatur dan pH optimum menggunakan metode Bradford.Berdasarkan hasil penelitian diperoleh temperatur optimum enzim bromelin yang diisolasi dari batang nanas,adalah 65oC dengan aktivitas sebelum diamobilisasi sebesar 1,239 unit/menit dan sesudah diamobilisasimemiliki aktivitas sebesar 0,982 unit/menit; sedangkan pH optimum enzim bromelin adalah 6,5 dengan aktivitassebelum diamobilisasi sebesar 0,907 unit/menit dan sesudah diamobilisasi memiliki aktivitas sebesar 2,758unit/menit.. Kata kunci : amobilisasi, bromelin, nanas, karagenan
Studi In Silico : Aktivitas Antibakteri Senyawa Resveratrol Terhadap Bakteri Salmonella typhi Penyebab Demam Tifoid Sinaga, Jonathan Cavin Ezra; Kumaunang, Maureen; Tuther, Paulix
CHEMISTRY PROGRESS Vol. 17 No. 2 (2024)
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35799/cp.17.2.2024.55994

Abstract

ABSTRAK Demam tifoid merupakan salah satu penyakit infeksi yang menyerang sistem pencernaan oleh adanya aktivitas bakteri Salmonella typhi. Pengobatan demam tifoid menggunakan antibiotik yang dapat menimbulkan resistensi bakteri, sehingga penemuan senyawa sebagai alternatif obat demam tifoid masih terus dikembangkan hingga saat ini. Penelitian ini bertujuan untuk mengetahui pengaruh penambatan senyawa resveratrol terhadap pertumbuhan dari bakteri Salmonella typhi. Jenis penelitian ini menggunakan metode penelitian penambatan molekul (molecule docking). Hasil dari molecular docking didapatkan binding afiinity interaksi ligan dan reseptor yaitu -7.8 kkal/mol, maka senyawa resveratrol dapat sebagai penghambat bakteri Salmonella typhi.   ABSTRACT Typhoid fever is an infectious disease that attacks the digestive system due to the activity of Salmonella typhi bacteria. Treatment of typhoid fever uses antibiotics which can cause bacterial resistance, so the discovery of compounds as alternative drugs for typhoid fever is still being developed today. This research aims to determine the effect of anchoring the resveratrol compound on the growth of Salmonella typhi bacteria. This type of research uses the molecular docking research method. The results of molecular docking showed that the binding affinity of the ligand and receptor interaction was -7.8 kcal/mol, so the resveratrol compound could act as an inhibitor for Salmonella typhi bacteria.
Co-Authors Agnes Eri Maryuni Angmalisang, Ping Astony Audy Denny Wuntu Bobanto, Maria D. Cindy Kalangi Ferdy . Frans Augusthinus Asmuruf Gerald Tamuntuan Irmi Bangol Jein Damanis, Jein Jemmy Abidjulu Julius Pontoh Lidya I Momuat Lidya Irma Momuat Max Revolta John Runtuwene Meiske S Sangi Meiske S. Sangi Muzakir Rahalus, Muzakir Octolia Togibasa Pandara, Dolfie Paulus Sinaga, Jonathan Cavin Ezra Tuther, Paulix Unso, Kristina Vanda Kamu Vanda S Kamu Vanda S. Kamu Vanda Selvana Kamu Vistarani Arini Tiwow Yohanis Irenius Mandik Yohanis Mandik