Article Per Year (5 Year)
p-Index From 2021 - 2026
1.116
P-Index
This Author published in this journals
All Journal EUGENIA Jurnal Agroekoteknologi Terapan AGRI-SOSIOEKONOMI COCOS EKOTON
Edy F. Lengkong
Unknown Affiliation
Agriculture, Biological Sciences & Forestry Economics, Econometrics & Finance Environmental Science Social Sciences

Published : 15 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 4 Documents
Search
Journal : EUGENIA

 1 
STUDI PERAKITAN KELAPA HIBRIDA GSK x DMT BERDASARKAN PENANDA RAPD (RANDOMLY AMPLIFIED POLYMORPHIC DNA) Runtunuwu, Semuel D.; Novarianto, Hengky; Tampake, Heldering; Lengkong, Edy F.
EUGENIA Vol 14, No 1 (2008)
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35791/eug.14.1.2008.7431

Abstract

ABSTRACT   Runtunuwu, S.D. et al. 2008. Assembling Hybrid Coconut of GSK x DMT Based on RAPD (RANDOMLY AMPLIFIED POLYMORPHIC DNA) Marker. Eugenia 14 (1) : 134-152.   The aimed of this research was : 1. assembling hybrid coconut GSK x DMT (Genjah Salak x Dalam Mapanget) that seeds growth was relatifly homogeneous based on RAPD (Randomly Amplified Polymorphic DNA) marker and 2. to found the assembling method of hybrid coconut that will produce massive seeds relatifely short time will homogeneous plant. It was 65 individu trees observe for the average of famale flower per bunch. The result was 25 individu of coconut GSK has the average flower production > 40 per bunch was analyze the homogeneous genetic with the RAPD marker. Based on the analyze RAPD that were 25 individu of GSK coconut trees have the same genetic average 88 % and 14 individu among that was 100 % have same genetic. Further more that 14 individu of GSK was crossing with the 3 individu of DMT that have high yield per year its was DMT 1188, 1172 and 781. Based on the evaluation for the color of buds, high of buds, the steam circle, the petiole color and the germination time of hybrid coconut seeds from the crossing of GSK x DMT 1188 produce more than    70 % seeds that have same genetic, also for crossing of GSK x DMT 1172 have 9 combination and have more than 70 % that same genetic, 10 combination from crossing GSK x DMT 781 have more than 80 % same seeds growth. Therefore, using the RAPD marker were successfully produced 28 crossing of the hybrid coconut GSK x DMT that have relatifly homogeneous seeds growth.   Keywords : assembling, hybrid coconut GSK x DMT, RAPD.
IDENTIFIKASI KERAGAMAN GENETIK KENTANG SUPEJOHN TRANSGENIK (Solanum tuberosum L. var supejohn) Lengkong, Christine L.W.; Polii-Mandang, Jeany; Lengkong, Edy F.
EUGENIA Vol 18, No 3 (2012)
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35791/eug.18.3.2012.4095

Abstract

ABSTRACT   The aim of this study was to determine the genetic diversity of potato Supejohn transgenic that had been twenty-five times subcultured with exploration  method. The genetic variability was calculated using molecular marker Polimorphic Random Amplified DNA (RAPD) analysis of ten samples from four weeks planlets of Supejohn transgenic plants using ten random primers. DNA isolation of 10 samples using CTAB buffer  then measurement of the DNA qualification and quantification,  afterwards amplification of DNA by PCR using 10 random primers followed by electrophoresis on a 1% agarose gel with TAE 1x electrode buffer solution. Visualisation  the results used UV Transluminator to see DNA bands and the data of  polymorphic bands had been analized used the NTSYS-pc version of 1.07 program to obtain the similarity coefficient  and the dendrogram.The results showed that five random primers produced polymorphic DNA bands with 0.39 - 0.88 similarity coefficient  and the average similarity coefficient is 0.63 (63%) or the genetic diversity of the samples as many as 37%. Dendrogram formed eight distinct clusters corresponding similarity coefficient, the formation of clusters means that there is genetic diversity among the DNA samples. Keywords: potato Supejohn transgenic, RAPD, genetic diversity ABSTRAK Penelitian ini bertujuan untuk  menentukan keragaman genetik kentang Supejohn transgenik yang telah dua puluh lima kali disubkultur dengan menggunakan metode penelitian eksplorasi dengan penanda molekuler  Random Amplified Polimorphic DNA (RAPD) terhadap 10 sampel tanaman Supejohn transgenik, berumur empat minggu dan  menggunakan  10 primer random.  Isolasi DNA dari 10 sampel menggunakan buffer CTAB sesudah itu dilakukan pengukuran kualitas dan kuantitas  DNA, kemudian dilanjutkan dengan amplifikasi DNA secara PCR menggunakan 10 primer random diikuti dengan  elektroforesis pada gel agarose 1% dengan larutan penyanggah elektroda TAE 1x. Visualisasi hasil elektroforesis  menggunakan UV Transluminator untuk melihat pita DNA  dan analisis  data pita polimorfik  menggunakan program NTSYS-pc versi 1.07 sehingga didapatkan koefisien kesamaan dan dendrogram. Hasil penelitian menunjukkan bahwa lima primer random menghasilkan pita DNA polimorfik dengan koefisien kesamaan 0.39 – 0.88 dan rata-rata koefisien kesamaan yaitu 0.63 (63%) atau  keragaman  genetik sampel sebesar 37%.  Dendrogram  membentuk delapan cluster yang berbeda sesuai koefisien kesamaan,terbentuknya clusters mengartikan bahwa ada keragaman genetik antar sampel DNA. Eugenia Volume 18 No. 3  Desember 2012 Kata kunci : kentang Supejohn transgenik , RAPD, keragaman genetik
PENGGUNAAN PENANDA MOLEKULER RANDOM AMPLIFIED POLYMORPHIC DNA (RAPD) UNTUK ANALISIS KERAGAMAN GENETIK KELAPA WEST AFRICAN TALL (WAT) Lengkong, Edy F.; Runtunuwu, S. D.
EUGENIA Vol 11, No 3 (2005)
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35791/eug.11.3.2005.7391

Abstract

ABSTRACT Lengkong, E.F. and S.D. Runtunuwu. 2005. Use of Molecular Marker Random Amplified Polymorphic DNA (RAPD) to Genetic Diversity Analysis of West African Tall (WAT) Coconut. Eugenia 11 (3):210-217.   Information on genetic diversity of crop gemplasm has several important implications for plant breeder. Among others is to help the breeders to decide what sources to cross so as to making new genetic combination. The use of molecular marker to genetic diversity analysis on DNA level is usefull, because it provides an opportunity to more precisely measure genetic relationships as well is not affected by the environment. The objective of this research was to analyze genetic diversity of West African Tall (WAT) using molecular marker RAPD. Five arbitrary 10-mer primers were used to amplify total DNA genom, and to generate 44 band DNA with 26 band or 59 % were polymorphic band. It was revealed that genetic diversity within population of WAT coconut was 14 %. Based on cluster analysis, at the genetic similarity 85% or genetic diversity 15 % the population was separated on three clusters. This research concluded that the population of WAT coconut grown from open pollinated seeds has different genotype one each others. Keywords: West African Tall (WAT), Coconut, RAPD
STUDI KERAGAMAN POLA PITA DNA DAN KESERAGAMAN POPULASI KELAPA GENJAH SALAK (GSK) BERDASARKAN PENANDA RAPD Hengky, Novarianto; Runtunuwu, Semuel D.; Lengkong, Edy F.; Singkoh, Merlyn
EUGENIA Vol 13, No 1 (2007)
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35791/eug.13.1.2007.7430

Abstract

ABSTRACT Novarianto, H., et.al. 2007. The Study of the Variety of DNA Tape Form and the Coconut Genjah Salak Population Variety Based on RAPD Code. Eugenia 13 (1):109-118.   The goal of this research to study the variety of DNA tape form and the population variety of coconut Genjah Salak (GSK) based on RAPD code (Randomly Amplified Polymorphic DNA). 25 trees individu of coconut GSK was amplification the genomic of DNA using 35 primer Operon Kit A and Kit B. The coconut GSK individu tree that were analyse was selected based on the female flower production, with average above 40 female flower. The electrophoresis of DNA result of amplication PCR get tape form monomorfic and polimorfic between 25 individu coconut GSK trees that was analyzed. OPA 09 primer and OPB 06 get the result of monomorfic tape form, other wise the OPA 13 primer, OPB 05 and OPB 10 get polimorfic tape form. The genetic similarity in coconut Genjah Salak (GSK) population based on RAPD code rate was 88 % or have the variety genetic of 12 %. Therefore based of this similarity the coconut GSK population was clustering in five groups. The goal of this research to study the variety of DNA tape form and the population variety of coconut Genjah Salak (GSK) based on RAPD code (Randomly Amplified Polymorphic DNA). 25 trees individu of coconut GSK was amplification the genomic of DNA using 35 primer Operon Kit A and Kit B. The coconut GSK individu tree that were analyse was selected based on the female flower production, with average above 40 female flower. The electrophoresis of DNA result of amplification PCR get tape form monomorfic and polimorfic between 25 individu coconut GSK trees that was analyzed. OPA 09 primer and OPB 06 get the result of monomorfic tape form, other the OPA 13 primer, OPB 05 and OPB 10 get polimorfic tape form. The genetic similarity in coconut Genjah Salak (GSK) population based on RAPD code rate was 88 % or have the variety genetic of 12%. Therefore based of this similarity the coconut GSK population was clustering in five groups. Keywords: Keragaman genetic; kelapa Genjah Salak (GSK), Randomly Amplified Polymorphic DNA (RAPD)
Co-Authors Arfa ., Dondo Arthur G. Pinaria Christine L.W. Lengkong Dondo, Arfa ., Doodoh, Beatrix Frangky J. Paat Gabriel M. R. Sondakh Galensong, Justio P.D. Giavirna J. Enoch Heldering Tampake Hengky Novarianto Inkiriwang, Annatje E. B. Inkiriwang, Annatje E.B. James B. Kaligis Jantje Pongoh Jeanne M. Paulus Jeany Polii-Mandang Jemmy Najoan Jemmy Najoan, Jemmy Johannes E. X. Rogi Juita S. Sinaulan Malintoi, Friska Martha Fera Garing Max ., Tulung Merlyn Singkoh Mersi M. Tanawani Nangoi, Ronny Novarianto Hengky Ogie, Tommy B. Pinaria, Arthur ., Polii, Maria G. M. Rantung , Rantung Robert W. Tairas S. D. Runtunuwu saartje Sompotan Semuel D. Runtunuwu Stanley A.F. Walingkas Stella Tulung Supit, Paula C. H. Tegar P. Prok Tilaar, Wenny ., Tulung, Max ., Tulung, Stella Maria Theresia Ventje Pangemanan Walingkas, Stanley A. F. Wenny Tilaar