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All Journal Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Jurnal Produksi Tanaman AGRIVITA, Journal of Agricultural Science BERITA BIOLOGI Proceedings of Annual Conference for Muslim Scholars IJOBI

Endang Gati Lestari

Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian Jl. Tentara Pelajar 3A, Bogor 16111

Author-ID : 306201

Religion Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Economics, Econometrics & Finance Environmental Science Immunology & microbiology Neuroscience Social Sciences Other

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Title

INDUKSIKALUS DAN REGENERASI TUNAS PULAI PANDAK (Rauwolfta serpentina L.) Yunita, Rossa; Lestari, Endang Gati
BERITA BIOLOGI Vol 9, No 1 (2008)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v9i1.807

In vitro culture can be applied for producing new genotype which is tolerant to biotic and abiotic or to incerase secondary metabolic content. To obtain the optimum result of variety improvement, regeneration system should firstly be found out.It is sufficiently difficult to regenerate pulai pandak (Rauwolfia serpentina L.). Hence, with this system, the improvement of R. serpentina with secondary metabolic content higher than the other. The mother stok of R. serpentina used in this experiment, belongs to the collection of BB-Biogen. Calli were produced from leaves and internodes which is cultured at medium MS contain 2.4-D (0, 1, 3,5, 7 mg/1) combined with caseine hydrolysate 3 mg/1. Regeneration medium was MS contain BA (0,5, 1 mg/1) combined with zeatin (0, 0.1 and 0.5 mg/1) and root formation used was three kinds of auxin (IBA, IAA and NAA). The result showed that inter nodels was better that leaves to callus induction. In this experiment, MS + 2,4-D 1 mg/1 + CH 3 mg/1 was the best medium to induct calli,while medium MS + BA 1 mg/1 + Zeatin 0,5 mg/1 + maltosa 3% to regenerate and MS + IBA lmg/1 for root induction.

MEKANISME TOLERANSI DAN METODE SELEKSI TUMBUHAN YANG TAHAN TERHADAP CEKAM AN KEKERINGAN Lestari, Endang Gati
BERITA BIOLOGI Vol 8, No 3 (2006)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Water is the main parameter to determine whether the yield potential of a plant is obtained or not. Water deficit on the tissue causes the disruption of all chemical process in the plant metabolism resulted in the plant growth impediment.In order to acquire the drought tolerance variety, various efforts have been conducted. Among others, in addition to the selection and characterization of the available germ plasma along with its cross-breeding, the development of the drought tolerance plant is conducted through somaclonal varitype induction. In the cultivation of the drought tolerance plant, beside physiological mechanism and biochemistry related to the plant tolerant to drought, the procedure of the selective and optimal selection should be managed.Hence,the mastery of the optimum selection techniques, in a relatively short time, the new drought tolerance lines could be obtained.

PRODUKSI BIBIT KENCUR (Kaempferia galanga L.) MELALUI KULTUR JARINGAN Lestari, Endang Gati; Hutami, Sri
BERITA BIOLOGI Vol 7, No 6 (2005)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

To increase domestic and international demand of Kencur (Kaempferia galanga L.) makes this plant potentially develop.Is traditionally used to keep the body warm, as analgetic and expectorant. In the attemp of providing adequately and qualitatively uniformed supply, in vitro experiment has been conducted at BB-Biogen (Indonesian Center for Agricultural Biotechnology and Genetik Resources Research and Development).The selected rhizomes was used as explant. The experiment was orthogonally arranged consisting of MS vitamin and B5, and BA ( 0, 3 and 5 mg/1) and thidiazuron 0,1 mg/1. This experiment comprised three activities, they were shoot initiation, shoot multiplication and acclimatization. The result showed that MS + BA 3 mg/1 + thidiazuron 0,2 mg/1 could induce shoot formation. From the applied media, it was shown that the addition of MS vitamin at the MS basic media and BA 3 and 5 mg/1 added with thidiazuron could result the most optimum shoot, leaves and roots and was not significantly different from the addition of B5 vitamin at basic media of MS + BA 3 and 5 mg/1, 6.9 shoot was averagely produced in this media. The shoot could generate such an adequate number of root that it could be directly acclimatized. The acclimatized plantlet in the green house uses the mixture of soil and manure with the ratio of 1:1 can optimally grow.

Co-Authors Ani Kurniawati Bernadetta Octavia Chaerani ,, Chaerani Ika Mariska Istifhama, Lia Iswari Saraswati Dewi Metafisika, Kartika Mohamad Rahmad Suhartanto Muhamad Syukur Nafisah, Aulia Erta Nisa, Masayu Nessya Khoirun RAGAPADMI PURNAMANINGSIH ROHIM FIRDAUS Rosa Yunita ROSSA YUNITA Sri Hutami Suci Rahayu Sumeru Ashari Syarifah Iis Aisyah

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