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UJI TOKSISITAS EKSTRAK DAUN HYPERICUM LESCHENAULTII CHOISY DARI GUNUNG LAWU TERHADAP ARTEMIA SALINA L. SEBAGAI KANDIDAT ANTIKANKER Noviana, Yudha; Ayudewandari Pranatami, Dwimei
At-Taqaddum Volume 11, Nomor 2, Tahun 2019
Publisher : Quality Assurance Institute (LPM) State Islamic University Walisongo Semarang

Mount Lawu is a biodiversity rich mountain, with the potensial of medicinal plants of various kinds including anticancer plants. Flora on the Mount Lawu that has potential for anticancer is Hypericum leschenaultii Choisy containing compounds hypericin. Hypericum has several biological activities such as a antioxidant, antitumor, and anticancer. The research aims to determine the potential of bioactive compounds and extracts of the leaves of H. leschenaultii from Mount Lawu and toxicity to Artemia salina Leach and profile Thin Layer Chromatography (TLC) it. Ethanol extract of the leaves of H. leschenaultii was tested toxicity with Brine Shrimp Lethality Test (BST) using a concentration of 400 µg/ml, 200 µg/ml, 100 µg/ml, 50 µg/ml, 25 µg/ml. The TLC profiles of bioactive compounds were determined by using spesific spray reagent. The results show the potential and toxicity of leaf extract of H. leschenaultii with LC values 175,77 µg/ml. TLC profile of bioactive compounds leaf extract of H. leschenaultiicontaining flavonoids with Rf values of 0,98 and terpenoid compounds with Rf value of 0,91; 0,95; 0,98. ==================================================Gunung Lawu merupakan gunung yang kaya akan keanekaragaman hayati, dengan potensi tumbuhan obat yang beragam, termasuk potensi tumbuhan sebagai antikanker. Adanya potensi tumbuhan sebagai sumber senyawa antikanker tersebut belum banyak diteliti dan digunakan sebagai pengobatan alternatif. Flora di Gunung Lawu yang potensial sebagai tumbuhan antikanker, yaitu Hypericum leschenaultii Choisy yang mengandung senyawa hypericin dan memiliki status keberadaan yang melimpah di Gunung lawu, sehingga sangat penting untuk diketahui manfaatnya agar dapat dimanfaatkan lebih luas. Hypericin memiliki beberapa aktivitas biologis seperti antioksidan, antitumor, dan antikanker. Penelitian ini bertujuan untuk mengetahui potensi senyawa bioaktif ekstrak daun H. leschenaultii dari Gunung Lawu sebagai antikanker dengan melihat toksisitasnya terhadap Artemia salina Leach serta profil Kromatografi Lapis Tipis (KLT) nya. Untuk mengetahui potensi sebagai tanaman antikanker maka ekstrak etanol daun H. leschenaultii diuji toksisitasnya dengan metode Brine Shrimp Lethality Test (BST). Dalam uji BST tersebut digunakan 5 konsentrasi esktrak etanol daun H. leschenaultii yaitu: 400 µg/ml, 200 µg/ml, 100 µg/ml, 50 µg/ml, 25 µg/ml. Kandungan senyawa bioaktif dideteksi dengan reagen semprot spesifik untuk mengetahui profil KLT-nya. Hasil penelitian menunjukkan potensi dan toksisitas ekstrak H. leschenaultii dengan nilai LC 175,77 µg/ml. Profil KLT senyawa bioaktif ekstrak daun H. leschenaultii mengandung senyawa flavonoid dengan nilai Rf 0,98 serta senyawa terpenoid dengan nilai Rf 0,91; 0,95; 0,98.

PERBANDINGAN JUMLAH TOTAL BAKTERI PADA PENGGUNAAN WADAH PENYIMPANAN AIR SUSU IBU (ASI) YANG BERBEDA Pranatami, Dwimei Ayudewandari
Al-Hayat: Journal of Biology and Applied Biology Vol 3, No 1 (2020)
Publisher : Fakultas Sains dan Teknologi, UIN Walisongo Semarang

At the beginning of its life, breast milk is the best food for babies because breast milk contains all the nutrients that can be used for optimal growth and development of the baby. Therefore, the government has promoted exclusive breastfeeding programs for infants 0-4 months or up to 6 months if possible. However, the current phenomenon especially among working mothers, there are regulatory difficulties in giving milk to children. Breast milk can still be given by a working mother the pumping method and stored first. To facilitate the storage of breast milk, three types of containers are widely used, namely plastic clips, plastic bottles, and glass bottles. In the period of storage, it must be ensured that breast milk can be maintained with good quality and integrity. This study aims to compare the total number of bacteria that can grow in breast milk after being stored in three different types of containers. The sampling technique used in this study is total sampling. The treatment in this study was by placing breast milk in three different containers, namely plastic bottles (A), glass bottles (B), and plastic bags for storing breast milk (C) stored at room temperature (16-260C) with three replications. Breast milk is obtained from volunteers who have been predetermined and then accommodated in sterile containers. Statistical test results showed that the variation of the container did not affect the total bacterial colony with a p-value of 0.246 (P> 0.05) with the highest number of bacteria in plastic bag storage with an average of 5.4x103 CFU/ml.

PENGARUH PEMBERIAN DOSIS DAN FREKUENSI BIOFERTILIZER TERHADAP KADAR KLOROFIL DAUN BIBIT SENGON (Paraserianthes falcataria (L.) Nielsen) Dwimei Ayudewandari Pranatami; Sekar Arum
Indonesian Journal of Applied Sciences Vol 7, No 3 (2017)
Publisher : Universitas Padjadjaran

Klorofil merupakan komponen penting yang dibutuhkan dalam fotosintesis dimana pembentukanya diperlukan suplai zat hara seperti nitrogen dan fosfat. Suplai zat hara tersebut dapat dibantu dengan pemberian biofertilizer yang mengandung bakteri fiksasi nitrogen, bakteri pelarut fosfat dan mikroba dekomposer. Penelitian ini bertujuan untuk mengetahui pengaruh dosis dan frekuensi pemberian biofertilizer terhadap kadar klorofil bibit sengon (Paraserianthes falcataria (L.) Nielsen). Penelitian terdiri atas 2 perlakuan kontrol dan 8 perlakuan uji. Seri dosis biofertilizer yaitu 20, 40, 60, dan 80 mL/tanaman dengan frekuensi pemberian 1 minggu sekali dan 2 minggu sekali. Mikroba dalam biofertilizer terdiri atas Azotobacter chroococum, Azospirillum brasilense, Rhizobium leguminosarum, Bacillus subtilis, B. megaterium, B. licheniformis, Pseudomonas fluorescens, P. putida, Cellvibrio mixtus, Cellulomonas cellulans, Cytophaga saccharophila, Lactobacillus plantarum, dan Saccharomyces cerevisiae. Penelitian ini menggunakan rancangan acak lengkap (RAL) dan data dianalisis menggunakan uji ANAVA satu arah dengan uji lanjutan yaitu uji Duncan dan uji Brown –Forsythe dengan uji lanjutan Gomes Howell pada taraf 5%. Hasil penelitian menunjukkan bahwa pemberian dosis dan frekuensi biofertilizer berpengaruh nyata dalam peningkatan kadar klorofil daun.

UJI TOKSISITAS EKSTRAK DAUN HYPERICUM LESCHENAULTII CHOISY DARI GUNUNG LAWU TERHADAP ARTEMIA SALINA L. SEBAGAI KANDIDAT ANTIKANKER Yudha Noviana; Dwimei Ayudewandari Pranatami
At-Taqaddum Volume 11, Nomor 2, Tahun 2019
Publisher : Quality Assurance Institute (LPM) State Islamic University Walisongo Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21580/at.v11i2.4448

Mount Lawu is a biodiversity rich mountain, with the potensial of medicinal plants of various kinds including anticancer plants. Flora on the Mount Lawu that has potential for anticancer is Hypericum leschenaultii Choisy containing compounds hypericin. Hypericum has several biological activities such as a antioxidant, antitumor, and anticancer. The research aims to determine the potential of bioactive compounds and extracts of the leaves of H. leschenaultii from Mount Lawu and toxicity to Artemia salina Leach and profile Thin Layer Chromatography (TLC) it. Ethanol extract of the leaves of H. leschenaultii was tested toxicity with Brine Shrimp Lethality Test (BST) using a concentration of 400 µg/ml, 200 µg/ml, 100 µg/ml, 50 µg/ml, 25 µg/ml. The TLC profiles of bioactive compounds were determined by using spesific spray reagent. The results show the potential and toxicity of leaf extract of H. leschenaultii with LC values 175,77 µg/ml. TLC profile of bioactive compounds leaf extract of H. leschenaultiicontaining flavonoids with Rf values of 0,98 and terpenoid compounds with Rf value of 0,91; 0,95; 0,98. ==================================================Gunung Lawu merupakan gunung yang kaya akan keanekaragaman hayati, dengan potensi tumbuhan obat yang beragam, termasuk potensi tumbuhan sebagai antikanker. Adanya potensi tumbuhan sebagai sumber senyawa antikanker tersebut belum banyak diteliti dan digunakan sebagai pengobatan alternatif. Flora di Gunung Lawu yang potensial sebagai tumbuhan antikanker, yaitu Hypericum leschenaultii Choisy yang mengandung senyawa hypericin dan memiliki status keberadaan yang melimpah di Gunung lawu, sehingga sangat penting untuk diketahui manfaatnya agar dapat dimanfaatkan lebih luas. Hypericin memiliki beberapa aktivitas biologis seperti antioksidan, antitumor, dan antikanker. Penelitian ini bertujuan untuk mengetahui potensi senyawa bioaktif ekstrak daun H. leschenaultii dari Gunung Lawu sebagai antikanker dengan melihat toksisitasnya terhadap Artemia salina Leach serta profil Kromatografi Lapis Tipis (KLT) nya. Untuk mengetahui potensi sebagai tanaman antikanker maka ekstrak etanol daun H. leschenaultii diuji toksisitasnya dengan metode Brine Shrimp Lethality Test (BST). Dalam uji BST tersebut digunakan 5 konsentrasi esktrak etanol daun H. leschenaultii yaitu: 400 µg/ml, 200 µg/ml, 100 µg/ml, 50 µg/ml, 25 µg/ml. Kandungan senyawa bioaktif dideteksi dengan reagen semprot spesifik untuk mengetahui profil KLT-nya. Hasil penelitian menunjukkan potensi dan toksisitas ekstrak H. leschenaultii dengan nilai LC 175,77 µg/ml. Profil KLT senyawa bioaktif ekstrak daun H. leschenaultii mengandung senyawa flavonoid dengan nilai Rf 0,98 serta senyawa terpenoid dengan nilai Rf 0,91; 0,95; 0,98.

Edukasi Pola Hidup Bersih dan Sehat Pada Anak-anak di Masa Pandemi Dwimei Ayudewandari Pranatami; Hafidha Asni Akmalia; Ndzani Latifatur Rofiah
Bubungan Tinggi: Jurnal Pengabdian Masyarakat Vol 3, No 4 (2021)
Publisher : Universitas Lambung Mangkurat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/btjpm.v3i4.3643

Kemunculan virus Corona yang diawali dari tahun 2020 lalu membuat semua segi kehidupan terdampak. Penerapan protokol kesehatan menjadi salah satu cara yang paling ampuh untuk menekan angka kasus covid-19 di Indonesia. Tujuan dilaksanakan edukasi pola hidup bersih dan sehat pada anak-anak adalah untuk membimbing agar anak-anak lebih sadar dan berhati-hati dalam melakukan kegiatan di tengah pandemi sebagai bentuk pencegahan terhadap virus Corona. Pada kegiatan ini dilakukan edukasi terhadap anak-anak dengan metode pemberian materi, games dan praktik mencuci tangan dengan benar. Hasil kegiatan menunjukkan bahwa 90% anak dapat menjawab pertanyaan dan menyusun kata terkait pola hidup bersih dan sehat sehingga anak-anak mengetahui cara cuci tangan dengan baik, memilah sampah dan menjaga perilaku hidup sehat di rumah, di lingkungan serta di sekolah. Kegiatan ini juga diharapkan dapat mendorong kesadaran masyarakat secara umum untuk menerapkan Pola Hidup Bersih dan Sehat demi meminimasilasi dan mencegah penularan virus Corona; membantu pemerintah dalam menggalakkan program pola hidup bersih dan sehat di masa pandemi; dan menginisiasi keberlanjutan program pola hidup bersih dan sehat di masyarakat. The emergence of the Coronavirus, which began in 2020, has affected all aspects of life. Implementing health protocols is one of the most effective ways to reduce the number of COVID-19 cases in Indonesia. The purpose of implementing clean and healthy lifestyle education for children is to guide them so that they are more aware and careful in carrying out activities during a pandemic as a form of prevention against the Coronavirus. In this activity, education was carried out for children to provide materials, games, and direct practice. After attending the education, the children become aware of washing their hands, sorting waste, and maintaining a healthy lifestyle at home, in the neighbourhood, and at school. This activity is also expected to encourage public awareness, in general, to implement a Clean and Healthy Lifestyle to minimize and prevent the transmission of the Coronavirus; assist the government in promoting the Clean and Healthy Lifestyle program during the pandemic, and initiate the sustainability of the Clean and Healthy Lifestyle program in the community.

Perbandingan Jumlah Total Bakteri pada Penggunaan Wadah Penyimpanan Air Susu Ibu (ASI) yang Berbeda Dwimei Ayudewandari Pranatami
Al-Hayat: Journal of Biology and Applied Biology Vol 3, No 1 (2020)
Publisher : Fakultas Sains dan Teknologi, UIN Walisongo Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21580/ah.v3i1.6066

At the beginning of its life, breast milk is the best food for babies because breast milk contains all the nutrients that can be used for optimal growth and development of the baby. Therefore, the government has promoted exclusive breastfeeding programs for infants 0-4 months or up to 6 months if possible. However, the current phenomenon especially among working mothers, there are regulatory difficulties in giving milk to children. Breast milk can still be given by a working mother the pumping method and stored first. To facilitate the storage of breast milk, three types of containers are widely used, namely plastic clips, plastic bottles, and glass bottles. In the period of storage, it must be ensured that breast milk can be maintained with good quality and integrity. This study aims to compare the total number of bacteria that can grow in breast milk after being stored in three different types of containers. The sampling technique used in this study is total sampling. The treatment in this study was by placing breast milk in three different containers, namely plastic bottles (A), glass bottles (B), and plastic bags for storing breast milk (C) stored at room temperature (16-260C) with three replications. Breast milk is obtained from volunteers who have been predetermined and then accommodated in sterile containers. Statistical test results showed that the variation of the container did not affect the total bacterial colony with a p-value of 0.246 (P 0.05) with the highest number of bacteria in plastic bag storage with an average of 5.4x103 CFU/ml.

PERANAN ORGAN VESTIGIAL PADA MAMALIA dwimei ayudewandari pranatami
STIGMA: Jurnal Matematika dan Ilmu Pengetahuan Alam Unipa Vol 15 No 02 (2022)
Publisher : Universitas PGRI Adi Buana Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36456/stigma.15.02.5995.67-72

Living things undergo very complex growth and development. Mammals as a class in the kingdom animalia have organ growth, some of which leave residual organs or vestigial organs that still need to be studied more deeply about their role. Vestigial organs are often referred to as evidence of evolution and can be used as markers in the kinship of living things. The existence of vestigial organs in the mammalian body can be found in several places. The purpose of writing this article is to determine the role of vestigial organs in mammals. Many vestigial organs are considered to have no function, even though some of them actually support the survival of these living things. The vestigial organs that can still be found in mammals are: coecum, appendix, third eyelid, auricle muscle, wisdom teeth, splint bone, nails and spine and pelvic girdleThe vestigial organs still have a role even though not in a large part of them; become part of the lymphatic system, move muscles in mechanisms to maintain homeostasis, support other organs in the body and organ development to more complex stages. In addition to the role in this regard, the vestigial organs are still important to study because they are an important part of the growth and development of the body where its existence must have a meaning either to stop during the developmental period or to continue to have a role as long as the vestigial organs are present in the body.

INDEKS KEPUASAN MAHASISWA TERHADAP LAYANAN AKADEMIK DAN NON AKADEMIK UIN WALISONGO SEMARANG Anila Umriana; Dwimei Ayudewandari Pranatami
Jurnal Penjaminan Mutu Vol 8 No 1 (2022)
Publisher : UHN IGB Sugriwa Denpasar

Higher education as one of the places where education is held is expected to always maintain its quality and quality. To see the quality or quality of higher education management can be assessed from two indicators, namely the fulfillment of quality standards and the achievement of customer satisfaction. Universities must be able to manage their service quality system and administration because if not, the trust of higher education users will decrease as well as the quality of students as one of the university's users, so that student satisfaction measurements need to be carried out regularly to be input and improvement for the university. Student satisfaction survey was conducted on aspects of academic and non-academic services at UIN Walisongo Semarang. Academic and academic service aspects are each measured by 8 indicators. This study aims to determine the level of student satisfaction with service quality after participating in the learning process at UIN Walisongo Semarang. This study uses a quantitative approach and survey methods. The population of this survey is all students of UIN Walisongo Semarang. The results of the assessment of the student satisfaction index on academic services are as follows: the highest satisfaction is on the online guardianship indicator with an index of 2.99 and the lowest satisfaction is on the online lecture system (E-learning) indicator with an index of 2.27. Meanwhile, the results of the satisfaction index assessment on non-academic services are as follows: the highest indicators are; Religious guidance services for students (3.01), while the lowest index is; Career guidance and job information services (Walisongo Career Center) (2.69). All satisfaction results show high satisfaction criteria.

Education Power Satisfaction Index on UIN Walisongo Semarang Services Dwimei Ayudewandari Pranatami; Anila Umriana Umriana; Eka Vasia Anggis
Journal of Quality Assurance in Islamic Education (JQAIE) Vol 1 No 1 (2021): January - June
Publisher : Institut Agama Islam Negeri Sorong

Quality assurance is one of the benchmarks that can be seen in assessing the quality of a university. All efforts that can be made related to the quality assurance process must be carried out starting from the level of study programs to tertiary institutions and a thorough evaluation not only related to the learning curriculum but also the human resources involved in it. One of the essential human resources involved in the learning process is educators. The optimal performance of educators will affect the whole teaching and learning process, and the implementation of these educators is strongly influenced by satisfaction with campus services. So, this study aims to analyze the satisfaction of education personnel with the services provided by the campus. Surveying educators carried out the data search process in the UIN Walisongo Semarang. Nine aspects are analyzed by the level of satisfaction by educators, namely 1) Recruitment and Selection, 2) Employee Orientation and Placement, 3) Training, Learning and Development Tasks, 4) Job Evaluation 5) Career Paths 6) Welfare 7) Attendance and Assignment System, 8) Termination and 9) Campus Facilities. The results of the analysis obtained are that the satisfaction of education personnel is at a moderate to a very high level with the aspect that shows the highest satisfaction in the achievement and assignment system variable (3.50) and the element that is at the lowest level of satisfaction is the welfare variable (3.14).

PENGUATAN KARAKTER ANAK: PERILAKU MEMILAH SAMPAH DAN KETELADANAN AKHLAK NABI Ndzani Latifatur Rofi'ah; Dwimei Ayudewandari Pranatami; Hafidha Asni Akmalia; Dian Tauhidah
Martabe : Jurnal Pengabdian Kepada Masyarakat Vol 7, No 3 (2024): MARTABE : JURNAL PENGABDIAN KEPADA MASYARAKAT
Publisher : Universitas Muhammadiyah Tapanuli Selatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31604/jpm.v7i3.1152-1160

Sampah masih menjadi masalah bagi masyarakat di Indonesia. Perilaku memilah sampah menjadi salah satu alternatif solusi yang dapat diterapkan untuk mengurangi dampak masalah sampah terutama sampah plastik. Usia anak menjadi usia optimal untuk melatihkan karakter menjaga lingkungan, melalui perilaku memilah sampah. Penguatan karatkter dapat didukung dengan meneladani akhlak nabi dalam kehidupan sehari-hari. Pengabdian ini dilakukan dengan tujuan untuk menguatkan karakter anak melalui perilaku memilah sampah dan keteladanan sifat nabi. Kegiatan dilakukan dengan metode bermain dan belajar dengan teknik ceramah (penyampaian materi), menonton video, dan permainan. Hasil kegiatan dapat memberikan wawasan kepada anak-anak terkait dengan kegiatan pelestarian lingkungan melalui pemilahan sampah dan pembentukan karakter meneladani sifat nabi. Kegiatan berjalan dengan lancar, peserta mengikuti dengan antusias dan lebih memahami pentingnya menerapkan perilaku memilah sampah dengan diimbangi meneladani sifat nabi. Kegiatan serupa perlu dilakukan kembali hingga anak-anak dapat menerapkan pemahaman mereka dalam kehidupan sehari-hari.

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