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All Journal Jurnal Veteriner Majalah Kedokteran Bandung Majalah Obstetri dan Ginekologi Syntax Literate: Jurnal Ilmiah Indonesia ISM (Intisari Sains Medis) : Jurnal Kedokteran Berkala Penelitian Hayati Folia Medica Indonesiana
Hamdani Lunardhi
Department Of Andrology, Faculty Of Medicine, Universitas Airlangga, Dr. Soetomo Hospital, Surabaya
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Education Environmental Science Health Professions Immunology & microbiology Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Social Sciences Veterinary Other

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In vitro fertility test of human spermatozoa membrane protein fertilin beta antibody in mice (mus musculus balb/c) as immunocontraceptive candidate I'tishom, Reny; Soebadi, Doddy M; Hinting, Aucky; Lunardhi, Hamdani; Yudiwati, Rina
Folia Medica Indonesiana Vol. 52, No. 3
Publisher : Folia Medica Indonesiana

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Abstract

One of the materials as potential candidates immunocontraception material is spermatozoa. Fertilin beta is spermatozoa membrane protein and is found only in mature spermatozoa and ejaculate, which serves as an adhesion molecule. Spermatozoa membrane protein that is used as an ingredient immunocontraception candidate, must have specific criteria that the specificity of spermatozoa, the role of antigen in the fertilization process, which includes the formation of immunogenicity sufficient antibody response has the potential to block fertilization. Antibodies against spermatozoa affect the stages before fertilization of the reproductive process and can hinder the development of the embryo after fertilization. Until now very little research data spermatozoa membrane protein as an ingredient immunocontraception are up to the test of experimental animals. The research objective is to prove the role of the resulting antibody induction of antibodies fertilin beta protein in the membrane of human spermatozoa induce agglutination and reduce motility thus reducing the number of in vitro fertilization. Research conducted at the IVF Laboratory, Department of Biology of Medicine, Faculty of Medicine, University of Airlangga. This research includes: Test the potential of antibody protein beta fertilin membrane of human spermatozoa and inhibit the role of antibodies in vitro fertilization in mice (Mus musculus Balb/c). In vitro studies have resulted in fertilization figure of 25% is smaller than the number that is equal to control fertilization of 58.7%, whereas previously the spermatozoa were incubated first with a beta membrane protein antibody fertilin human spermatozoa. While the percentage of inhibition of sperm to fertilize an oocyte by 33.75%. Potential imunokontraseptif considered effective if it decreased significantly (P <0.05) than the numbers fertilization in the treatment group compared with the control group. This shows fertilin beta membrane protein antibody has the ability to inhibit human spermatozoa to fertilize oocytes that reduce the number of fertilization.
Effect of In-Vitro Alpha Lipoic Acid Addition on Spermatozoa Motility in Sperm Preparation Process Buanayuda, Gede Wira; Lunardhi, Hamdani; Mansur, Indra Gusti
Folia Medica Indonesiana Vol. 55, No. 4
Publisher : Folia Medica Indonesiana

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Infertility is a problem for husband and wife, in the last 20 years the number of infertile couples has tended to increase by around 6.5 million pairs. The infertile couple can use the intrauterine insemination method to obtain offspring if a conventional method approach cannot be performed. Insemination requires a sperm preparation stage in which there are centrifugation and resuspension procedures that tend to produce excess reactive oxygen species (ROS). Excessive ROS will damage the motility of the spermatozoa. This study aims to prove the addition of alpha lipoic acid (ALA) as an antioxidant in the process of sperm preparation to improve and maintain better sperm motility. This research is a laboratory study with an experimental research design. The sample consisted of 10 infertile men who visited the Andrology section of the Sayyidah Jakarta Mother and Child Hospital (RSIA), where each ejaculate from the patient would be divided into 3 groups namely (k1) fresh semen as a control group, (k2) sperm preparation group without ALA, (k3) group of sperm preparation with the addition of ALA. The motility of spermatozoa was observed with the WHO 1999 method for 4 hours in units of percent. Progressive motility in k3 (47.95 ± 3.617) was higher than in k2 (38.05 ± 3.278) statistically significantly different after 3 hours of observation (p<0.0001). Progressive motility in k3 (78.8 ± 5.841) was higher than k1 (56.55 ± 7.511) from the initial observation (p <0.0001). The progressive motility of k2 (76.05 ± 6.768) was higher than k1 (56.55 ± 7.511) from the start of the observation (0.0001). It can be concluded that the addition of ALA in the sperm preparation process increases and maintains progressive motility that is better than sperm preparation without ALA addition after 3 hours of observation.
Co-Authors Amarudin Amarudin Ashon Sa’ad Ashon Sa’adi Aswin, R. Haryanto Aucky Hinting Audia Mumtaz Rifasky Ayang Halim Budi Santoso Chitra Devi Paramita Prasetyawati Doddy M Soebadi Faizah, Zakiyatul Fayka Putri Poempida Gede Wira Buanayuda Ginting, Aucky Hendy Herdarto I'tishom, Reny Indra Gusti Mansur Jimmy Yanuar Lutfi Hardiyanto Mansur, Indra Gusti Ninik Darsini Puspa Wardhani Ramadhona, Nur Hafiz Relly Y. Primariawan RINA YUDIWATI BM, RINA Samsulhadi Samsulhadi Sukarjati Sukarjati Supardi Supardi Supardi Supardi Suyono, Seso Sulijaya Wahyuning Ramelan Widjiati Widjiati