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All Journal Majalah Obstetri dan Ginekologi Biomolecular and Health Science Journal Indonesian Journal of Clinical Pathology and Medical Laboratory (IJCPML) Majalah Biomorfologi (Biomorphology Journal) Jurnal Kedokteran Mulawarman Indonesian Andrology and Biomedical Journal Folia Medica Indonesiana

RINA YUDIWATI BM, RINA

Department Of Medical Biology, Faculty Of Medicine, Airlangga University, Surabaya, Indonesia

Author-ID : 3289663

Biochemistry, Genetics & Molecular Biology Health Professions Immunology & microbiology Medicine & Pharmacology Neuroscience Public Health

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HIPOSPADIA SUBKORONAL PADA PASIEN SINDROM KLINEFELTER ANAK Rezia Octarina; Tjahjo Djojo Tanojo; Rina Yudiwati
Jurnal Kedokteran Mulawarman Vol 7, No 1 (2020): Jurnal Kedokteran Mulawarman
Publisher : Fakultas Kedokteran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30872/j.ked.mulawarman.v7i1.3860

Sindrom Klinfelter merupakan kelainan kromosom seks yang banyak ditemukan pada laki-laki. Sindrom Klinefelter umumnya muncul pada masa pubertas dan dewasa dengan ciri khasnya: infertilitas, testis kecil, ginekomastia dan mikropenis. Anak-anak dengan Sindrom Klinefelter dapat memiliki manifestasi klinis mikropenis, hipospadia, kriptorkismus atau keterlambatan perkembangan. Namun, hipospadia merupakan manifestasi klinis yang jarang ditemukan pada sindrom ini. Pada laporan kasus ini, kami melaporkan satu kasus seorang anak laki-laki yang memiliki hipospadia dan disertai kelainan kromosom 47, XXY. Laporan kasus ini menggambarkan Sindrom Klinefelter pada anak-anak dengan manifestasi klinis hipospadia, skrotum bifidum, kriptorkismus dan DSD di mana hal ini merupakan manifestasi klinis yang jarang ditemui pada kelainan ini.

The Effect of Antioxidant Vitamin C and E Administration on Spermatozoa Quality of White Rat (Rattus Norvegicus) Strain Wistar Male Against Allethrin Exposure Faisal Yusuf Ashari; Rina Yudiwati; R.Haryanto Aswin
Biomolecular and Health Science Journal Vol. 1 No. 2 (2018): Biomolecular and Health Science Journal
Publisher : Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia

Introduction: Allethrin is an active ingredient used in anti-mosquito drugs. Allethrin enters the body through inhalation, allegedly can disrupt the quality of spermatozoa by decreasing its amount, motility, viability and morphology. Many studies suggested that with sufficient intake of vitamin C and E would be able to minimize free radicals caused by allethrin. This study aims to investigate the effect of giving antioxidant vitamin C and E on spermatozoa quality of white rat (Rattus norvegicus) strain Wistar male with allethrin exposure.Methods: This research was a post-test design randomized control group design using Rattus novergicus male strains Wistar exposed to allethrin (LPB IPB veterinary stem cell). A total of 16 white rat strains Wistar male divided into 4 groups, one control group (without any treatment) and 3 treatment groups (given antioxidant vitamin C, vitamin E and both). The exposure of mosquito coils was performed 8 hours per day for 45 days and on the 46th day, spermatozoa was observed. The data were analyzed using ANOVA one way and BNT.Results: The results showed that treatment with vitamins affected the amount, motility and viability of rat spermatozoa exposed to allethrin. The mean amount of control group spermatozoa and treatment was 14,93, the mean spermatozoa motility control and treatment groups were 45,13 and the mean spermatozoa viability of control and treatment groups was 58,88. This is shown by the real difference between the control group and the treatment (p <0,05). Similarly, in the control group found morphological abnormalities.Conclusion: Exposure of mosquito coils 8 hours/day for 45 days decreased the spermatozoa quality. Overall, the admission of antioxidant vitamin C and E are able to maintain the quality of spermatozoa instead of one type of vitamin.

THE COMPARISON OF CIGARETTE CONSUMPTION TOWARDS SEMEN ANALYSIS IN ANDROLOGY POLYCLINIC OF DR. SOETOMO GENERAL ACADEMIC HOSPITAL, SURABAYA, INDONESIA IN 2017 Yohanes Widyakusuma Eka Saputra; Kristanti Wanito Wigati; Rina Yudiwati; Ni Wajan Tirthaningsih
Majalah Biomorfologi Vol. 31 No. 2 (2021): MAJALAH BIOMORFOLOGI
Publisher : Universitas Airlangga, Surabaya, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/mbiom.v31i2.2021.31-38

Background: The habit of smoking tobacco is a lifestyle that has become a necessity for some people. Sometimes, social needs is the main reason for someone to have a smoking habit. Based on this problem, the level of cigarette consumption in the community has increased. Nowadays, the total number of couples visiting an infertility clinic is on the rise. This have led to an assumption among the community that smoking tobacco is one of the main factors causing male infertility. Objective: to compare light smokers and heavy smokers towards the results of semen analysis in infertile male patients. Materials and Methods: This study was an observational analytics, with a retrospective-cross sectional. The instrument used in this study was taken from medical records of the patients who visited the Andrology Clinic of Dr. Soetomo General Academic Hospital, Surabaya, Indonesia, for the period from January to December 2017. The observed semen analysis parameters were concentration, motility, and morphology for microscopic examination and liquefaction, pH, and volume for macroscopic examination, by following the inclusion and exclusion criteria. Results: Ninety-two samples of infertile male participants (50 light smokers and 42 heavy smokers) were included in this study. Semen analysis on its concentration, motility, spermatozoa morphology, as well as liquefaction, pH, and volume) towards the number of cigarette consumption showed no significant difference (p>0.05) between light smokers and moderate smokers. Conclusion: There was no significant difference between the light smoker and heavy smoker groups on the results of the semen analysis.

Human Sperm Cells After Purification Using SCLB Can Be Stored at 4o, -20o, or -80oC Before Small RNA Isolation Berliana Hamidah; Ashon Sa'adi; Rina Yudiwati
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 26, No 2 (2020)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v26i2.1530

There have been many studies about pre-analysis for sperm RNA examination which compared sperm purificationmethods, RNA isolation methods, sequencing methods, and semen storage before analysis. However, there is a lack ofstudies that determine the ideal storage temperature after sperm cell purification before RNA analysis, especially small RNAanalysis. The aim of this study was to determine the preferred storage temperature for human sperm cells after spermpurification using Somatic Cell Lysis Buffer (SCLB) before sperm small ribonucleic acid (RNA) isolation and analysis. Thisstudy was a true laboratory experiment using the post-test only control group design. The samples were 13 fresh humansemen that has been purified using SCLB. The sperm cells were then diluted and divided into four aliquots with differenttreatments. The first aliquot that served as a control group was immediately purified while the other three aliquots were0 0 0 stored for seven days at different temperatures as follows: 4 C, -20 , and -80 C. After the small RNA isolation, RNA levelbetween each group was compared. Micro volume spectrophotometer measured RNA level. The median of small RNA6 yields of the control group was 49.8 (5.33-522.46) ng/10 sperm cells. There was no significant difference in median of smallRNA yields of the control group and that of other groups. The median of the other groups with storage temperature0 0 0 6 of 4 C, -20 , and -80 C was 41.09 (7.03-1448.31), 65.95 (7.99-301.16), and 76.42 (10.45-434.25) ng/10 sperm cells,respectively (p-value= 0.314; α=5%). This condition suggested that after purification using SCLB, human sperm cells can be0 0 0 stored at temperatures of 4 C, -20 , or -80 C for seven days, depending on each laboratory facility.

Case Report : A Woman 27 Year Old with Mosaic Turner Syndrome Associate Hypogonadotropic Hypogonadism Cennikon Pakpahan; Bella Amanda; Berliana Hamidah; Rina Yudiwati
Indonesian Andrology and Biomedical Journal Vol. 1 No. 2 (2020): December
Publisher : Universitas Airlangga

Background: Turner syndrome is a chromosomal abnormality found in phenotypically women who have one intact X chromosome and the absence of second sex chromosomes. Case: The patient is a 27-year-old Javanese girl. At the age of 14 years, she came to the gynecology clinic with chief complaints of amenorrhea and then given cycloprogynova by the physcian, the complaint improved but menstruation did not appear in the following month did not return to the gynecologist for evaluation. At the age of 26, she returned to the gynecologist and then was given medicine to stimulate menstruation and was referred to an internist-endocrinologist because of a suspected hypothyroid. Then a few months later the patient returned to the gynecologist and then examined hormones, ultrasound, and karyotyping for evaluation as well as establishing the diagnosis. FT4 hormone examination results 0.87 ng / dl, TSHs 0.708 Uiu / mL, T3 (Total) 0.49 ng / mL, FSH 2.38 Miu / mL, LH 1.3 Miu / mL, prolactin 14.7 ng / ml, progesterone <0.10 ng / ml, estradiol <5 ng / ml indicates hypogonadotropic hypogonadism with hypothyroidism. Ultrasound results showed hypoplasia with uterine axis measuring 2.27 x 2.09 cm. Karyotyping result with 45x / 46xx indicate mosaic turner syndrome. Patients were given progestin, esthero and also thyrax for hormone replacement therapy. Discussion: Turner syndrome with hypogonadotropic hypgonadism is a rare variation of the turner syndrome. The definite cause is still uncertain. Some cases are usually accompanied by other hormone disorders such as thyroid and growth hormone. Conclusion: A 27-year-old woman with hypgonadotrophic hypgonadism associated with mosaic turner syndrome. The diagnosis is done by history taking, hormone examination, imaging, and karyotyping. treatment given progestin, estrogen, and thyrax.

Correlation Between Gender, Physical Activity, Family Obesity History and Consumption of Fruit or Vegetable with Obesity in Medical Students in Surabaya made sukma putra; Lilik Djuari; Rina Yudiwati
Indonesian Andrology and Biomedical Journal Vol. 3 No. 1 (2022): June
Publisher : Universitas Airlangga

The incidence of obesity in the world has increased recently and doubled from 1980 to 2014. Obesity can increase the risk of many diseases. Medical students are a vulnerable group affected by obesity because of stressful conditions that can increase the incidence of obesity. This analytical research uses a cross sectional method approach using primary data. The populations in this study were medical students at the Faculty of Medicine, Airlangga University. In this study, 300 samples were taken from first-fifth semester students. The independent variables that will be studied in this study are Gender, physical activity, family obesity history, and consumption of fruit or vegetables. The dependent variable in this study is nutritional status. Based on this study found a significant association between gender, family history of obesity, eating of fruits or vegetables to nutritional status. Still, there is no significant association between physical activity on the nutritional group of medical students. Three variables in this research correlated with the nutritional status of medical education students at the Faculty of Medicine, Airlangga University, Surabaya, which are gender, family obesity history, and consumption of fruit or vegetables. From this research, it was found that the incidence of over-nutrition was still high among medical education students and there was still a lack of awareness among students to exercise and consume fruit or vegetables in their meals.

Factors in infertility score on referral and non-referral cases who underwent In Vitro Fertilization (IVF) treatment at a fertility clinic in Surabaya, Indonesia Nadhif Rashesa Brahmana; Ashon Sa’adi; Rina Yudiwati
Majalah Obstetri & Ginekologi Vol. 30 No. 2 (2022): August
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/mog.V30I22022.58-65

HIGHLIGHTS 1. Infertility score should be identified to obtain successful outcome of In Vitro Fertilization (IVF).2. Factors considered in determining the infertility score were examined.3. Each factor in infertility score (e.g, age, duration of infertility, menstrual cycle, pelvic pain etc.) has its own different degrees of severity that influence the outcome of IVF. ABSTRACT Objectives: To examine the factors used in infertility score on referral and non-referral cases who underwent IVF treatment at Fertility Clinic of Graha Amerta, Dr. Soetomo General Academic Hospital, Surabaya, Indonesia, in 2014-2016 Materials and Methods: This research was a descriptive analytic retrospective study using secondary data from medical records at Fertility Clinic of Graha Amerta in 2014-2016. With a total sampling technique, a total of 342 referral and non-referral infertility cases were obtained. The variables in this study were the factors considered in infertility score. Results: From a total of 342 cases, 44% had the wife's age in the range of 30-35 years, 80.1% had an infertile duration of > 3 years, and 54.7% had one type of pelvic pain, 68.4% still had regular menstrual cycles, and about 76% did not have a history of pelvic infection. On sperm analysis factor, there were 27.5% of men who fell into the severe category according to infertility score. There was a significant difference in the duration of infertility and sperm morphology in referral and non-referral cases. Conclusion: Infertility score can be useful because each case has its own infertility factors with different degrees of severity, which leads to infertility.

Human Sperm Cells After Purification Using SCLB Can Be Stored at 4o, -20o, or -80oC Before Small RNA Isolation Berliana Hamidah; Ashon Sa'adi; Rina Yudiwati
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol. 26 No. 2 (2020)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v26i2.1530

There have been many studies about pre-analysis for sperm RNA examination which compared sperm purification methods, RNA isolation methods, sequencing methods, and semen storage before analysis. However, there is a lack of studies that determine the ideal storage temperature after sperm cell purification before RNA analysis, especially small RNA analysis. The aim of this study was to determine the preferred storage temperature for human sperm cells after sperm purification using Somatic Cell Lysis Buffer (SCLB) before sperm small ribonucleic acid (RNA) isolation and analysis. Thisstudy was a true laboratory experiment using the post-test only control group design. The samples were 13 fresh human semen that has been purified using SCLB. The sperm cells were then diluted and divided into four aliquots with different treatments. The first aliquot that served as a control group was immediately purified while the other three aliquots were 0 0 0 stored for seven days at different temperatures as follows: 4 C, -20 , and -80 C. After the small RNA isolation, RNA level between each group was compared. Micro volume spectrophotometer measured RNA level. The median of small RNA6 yields of the control group was 49.8 (5.33-522.46) ng/10 sperm cells. There was no significant difference in median of small RNA yields of the control group and that of other groups. The median of the other groups with storage temperature 0 0 0 6 of 4 C, -20 , and -80 C was 41.09 (7.03-1448.31), 65.95 (7.99-301.16), and 76.42 (10.45-434.25) ng/10 sperm cells, respectively (p-value= 0.314; Î±=5%). This condition suggested that after purification using SCLB, human sperm cells can be 0 0 0 stored at temperatures of 4 C, -20 , or -80 C for seven days, depending on each laboratory facility.

Radiation effect of wireless fidelity (wi-fi) on oocyte number of oocyte stimulation in mice (mus musculus) Nurbayatin, Anita; Widjiati, Widjiati; Primariawan, Relly Yanuari; Poernomo, Bambang; Sulistiawati, Sulistiawati; Yudiwati, Rina
Folia Medica Indonesiana Vol. 53, No. 3
Publisher : Folia Medica Indonesiana

Show Abstract | Download Original | Original Source | Check in Google Scholar

Infertility is a problem experienced by some women and men around the world. Most infertility problems in women is caused by impaired reproductive organs or disrupted ovulation.. One factor that causes impaired oocyte maturation is wireless fidelity (Wi-Fi) radiation which has a radio frequency field of 2.45 GHz. The radiation may increase the activity of free radical cells through the fenton reaction pathways that cause infertility because of the disrupted oocyte development. This study aimed to determine the effect of Wi-Fi radiation on the number of oocytes. This was an experimental study using control group design. The subjects were 32 female mice selected through complete random sampling and divided into two groups: control (R0) and exposure (R1). Each group consisted of 16 mice. The Wi-Fi radio frequency used was 2.5 GHz. Mice (R1) were placed closer to Wi-Fi source (± 15cm), and there were two types of laptop PCs and 3G mobile phones connected to internet placed next to mice for 15 hours/28 days. The mice underwent a simultaneous cycle with intraperitoneal injection of PMSG and HCG. Furthermore, the mice were mated with vasectomized male monomatingly to induce ovulation. The fertilization pouch in both oviducts were observed for oocyte collection. The number of oocytes was calculated using an inverted microscope. There was a difference in the number of oocytes between control and exposure group. Statistical tests were analyzed using Mann Whitney U and resulted in significant values (p value = 0.00). No oocytes count in exposure group. In other words, the group underwent anovulation. In conclusion, Wi-Fi radiation affected the number of oocyte stimulation in mice. Therefore, it was important to minimize the risk factors that trigger electromagnetic radiation on reproductive health.

Impact of preparation using conventional and modified density gradient centrifugation methods on sperm concentration, motility and number of normal motile sperm recovery (nmsr) Yudiwati, Rina; Pramesti, MPBD; Agustinus, Agustinus; Pradana, E; Purwanto, Bambang
Folia Medica Indonesiana Vol. 53, No. 3
Publisher : Folia Medica Indonesiana

Show Abstract | Download Original | Original Source | Check in Google Scholar

Some preparation techniques, either conventional or advanced, have been provided. Advanced technique may overcome the limitations of conventional techniques. Recently, not all fertility clinics in Indonesia are able to provide advanced preparation techniques. Some techniques require expensive equipments and can only be used for intracytoplasmic sperm injection (ICSI). Some fertility clinics use a modified procedure, namely the combination of density gradient centrifugation with a swim-up method for the preparation of the sperm to be used in ART. This study aimed to determine whether the modified density gradient centrifugation, which is density gradient centrifugation followed by a swim-up, is able to yield better results than conventional density gradient centrifugation. This study was a laboratory experimental pre and pos-test control group design. Population was all adult men aged 21-40 years old and the sampling unit was the man donor's semen which fulfilled inclusion criterias, collected during the periode of the study. Sample size was eight. Sperm analysis were done before and after preparation in conventional and modified group. Descriptive comparation analysis have been used. This study obtained NMSR 7.9+5.5 million/ejaculate and recovery rate (RR) 27.66+11.8 %. RR was lower compared to RR obtained conventional DGC method. RR might be lower because in modified DGC samples undergo two steps selection while conventional DGC only one step selection. But conventional DGC samples should be centrifuged twice, therefore sperms might experience more trauma. Lower RR sperm does not exclude the possibility to be used for ART, because still within the required number for all TRBs. In conclusion, modified DGC preparation method obtained lower NMSR and RR, nevertheless harvested sperms can still be used in all kind of ART.

Co-Authors Agustinus Agustinus Ashon Sa'adi Ashon Sa’adi Ashon Sa’adi Aswin, R Haryanto Aswin, R. Haryanto Aucky Hinting Bambang Poernomo Bambang Purwanto Bella Amanda Bella Amanda Berliana Hamidah Berliana Hamidah Darsini, Ninik Doddy M Soebadi Faisal Yusuf Ashari Hamdani Lunardhi Hamidah, Berliana I'tishom, Reny Lilik Djuari made sukma putra Nadhif Rashesa Brahmana Ninik Darsini Nurbayatin, Anita Pakpahan, Cennikon Pradana, E Pramesti, MPBD Relly Yanuari Primariawan, Relly Yanuari Rezia Octarina Seso Sulijaya Suyono Sulistiawati Sulistiawati Suyono, Seso Sulijaya Tanojo, Tjahjo Djojo Tirthaningsih, Ni Wajan Widjiati Widjiati, Widjiati Wigati, Kristanti Wanito Yohanes Widyakusuma Eka Saputra

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