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TOXICOLOGICAL SCREENING OF ELLAGIC ACID IN POMEGRANATE FRUIT AND HYDROXYAPATITE COMBINATION AS BONE GRAFT MATERIAL ON BHK-21 FIBROBLAST CELL Agung Satria Wardhana; Isyana Erlita; Intan Nirwana; Hendrik Setiabudi
Dentino : Jurnal Kedokteran Gigi Vol 5, No 1 (2020)
Publisher : FKG Unlam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/dentino.v5i1.8132

Background: Bone graft is an alternative therapy for periodontitis and other bone destructive lesions. Several studies had revealed Ellagic Acid (EA) ability in increasing osteogenesis process. EA contains polyphenols, such as Ellagitannin, Gallotannin, and Anthocyanin, which demonstrate anti-inflammatory and antibacterial activity as well as growth factor stimulating effect. EA combination with bone graft material (hydroxyapatite) is anticipated to enhance bone osteogenesis yet no investigation was performed to identify its toxicity towards fibroblast cell. Objective: To analyze EA toxicity on fibroblast cell in vitro. Methods: This was a true experimental study using post-test only with control group design. Fibroblast cell was exposed with EA in eight different concentrations: 0.1%, 0.2%, 0.5%, 1%, 2%, 3%, 4% and 5%. Control group comprised of cell control and media group. All groups were exposed to MTT Assay test and measured using Elisa Reader. Result: The calculation of cell viability value in EA groups at 0.1%, 0.2%, 0.5%, 1%, 2%, 3%, 4% and 5% concentration were 88.2%, 92.3%, 97.5%, 89.5%, 84.2%, 90.7%, 88.9% and 89.4% respectively. Conclusion: All EA and hydroxyapatite combinations are not toxic towards BHK-21 fibroblast cells.

THE EFFECT OF LACTIC ACID AND ARTIFICIAL SALIVA SOLUTION IMMERSION TO THE RELEASE OF CALCIUM IONS ON BIOACTIVE RESIN Dewi Puspitasari; Nurah Tajjalia; Diana Wibowo; Agung Satria Wardhana
Dentino : Jurnal Kedokteran Gigi Vol 6, No 2 (2021)
Publisher : FKG Unlam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/dentino.v6i2.12004

Background: Bioactive resin can release calcium ions when contact with solution media, even in acid condition. In the oral cavity, pH may change into acid condition due to the metabolic results of Streptococcus mutans. The bacteria metabolize carbohydrates into organic acids, one of which is lactic acid. Purpose: Analyze the effect of lactic acid solution and artificial saliva on the number of the release of calcium ions of bioactive resin. Methods: Forty-two specimens (diameter 15 mm x thickness 1 mm; n= 7/group fabricated with Activa™ Bioactive Restorative (Pulpdent). The specimens that meet the criteria were divided into 6 groups. The specimen was immersed for 1 and 7 days in the incubator at 37oC. The number of calcium ion release is measured using titration method. Results: Two Way Anova test and Post Hoc Bonferonni test showed there were significant differences among all group for lactic acid 1 day (4.040 ± 0.360) µg, artificial saliva 1 day (0.640 ± 0.338) µg, distilled water 1 day (1.040 ± 0.504) µg, lactic acid 7 days (5.400 ± 0.312), artificial saliva 7 days (1.640 ± 0.215) µg, distilled water 7 days (3.520± 0.356 µg). Conclusion: There was an influence of lactic acid and artificial saliva on the number of calcium ion releases of bioactive resin. Immersion of bioactive resin in the lactic acid solution increase the calcium ion releases and artificial saliva decrease the calcium ion release compared to distilled water. Keywords: artificial saliva, bioactive resin, calcium ion release, lactic acid

EFFECT OF KARAMUNTING LEAF EXTRACT (Melastoma malabathricum) ON THE PROLIFERATION OF BABY HAMSTER KIDNEY-21 (BHK-21) FIBROBLAST CELLS Aanisah Ramadamayati; Muhammad Yanuar Ichrom Nahzi; Agung Satria Wardhana
Dentino : Jurnal Kedokteran Gigi Vol 7, No 1 (2022)
Publisher : FKG Unlam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/dentino.v7i1.13102

Background: Pulpitis is an inflammation of dental pulp due to infection of microorganisms from dental caries. Pulpitis is clinically divided into reversible and irreversible pulpitis. Treatment of reversible pulpitis can be done by applying a pulp capping material. The use of calcium hydroxide as a pulp capping material is still not optimal, so an alternative material is needed, namely karamunting leaf (Melastoma malabathricum). The secondary metabolites in karamunting leaf extract have antibacterial activity. Antibacterial activity is needed in the regeneration of pulp tissue. Purpose: To analyze the effect of karamunting leaf extract (Melastoma malabathricum) on viability and proliferation of Baby Hamster Kidney-21 (BHK-21) fibroblast cells using the MTT Assay method. Methods: This study is a purely experimental study with post test only with control group design which were divided into 8 groups. The group was treated with karamunting leaf extract consisted of 5 groups, were 5%, 10%, 15%, 20%, and 25% concentrations also 3 control groups, were positive control (calcium hydroxide), cell control, and media control. Absorbance value was read using ELISA Reader and the percentage of cell viability was calculated. Results: All treatment groups had cell viability values higher than cell control and cause proliferation of BHK-21 fibroblast cells. The One Way Anova showed a value of sig=0,000 (p<0,05), which means that there was a minimum difference in one treatment group. Conclusion: Karamunting leaf extract and calcium hydroxide can maintain viability and cause proliferation of BHK-21 fibroblast cells. Karamunting leaf extract 25% concentration more effective in causing proliferation of BHK-21 fibroblast cells.

Pengaruh Pasta Kelakai (Stenochlaena palustris) Konsentrasi 30% terhadap Kekasaran Permukaan Heat cured Acrylic Pratama Wicaksana Nugraha; Rahmad Arifin; Agung Satria Wardhana
Dentin Vol 6, No 2 (2022)
Publisher : Universitas Lambung Mangkurat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/dentin.v6i2.6388

ABSTRACTBackground: Tooth loss based on Riskesdas at the age of 35-44 years is 17.5% and increases at the age of 65 years and over (30.6%). Dentures are used to improve physical, psychological and social functions of people who have lost teeth. Denture cleaning is an important aspect of denture care to maintain oral health. Kelakai leaf extract paste can be used as an alternative denture cleaning paste because it has a low abrasive content and also an extract of kelakai leaf (Stenochlaena palustris) at a concentration of 30% has an average inhibition zone of 16.60 mm to Candida albicans. Objective: To determine the effect of 30% concentration of Kelakai extract paste on the surface roughness of heat cured acrylic. Methods: True experimental with pretest and posttest with control group design. The study used 27 samples divided into 3 treatment groups with 2 measurements. Group 1 is a negative control, group 2 is a positive control using toothpaste and group 3 is a paste extract treatment group. Results: The results of the Post Hoc Mann Whitney test analysis showed that there was a significant difference in the 30% concentration of mackerel extract paste with the control group. The results of this study also obtained an average surface roughness value of 0.090 m after giving treatment of extract paste. Conclusion: There was an effect of 30% concentration of Kelakai extract paste on the surface roughness of heat cured acrylic. Keywords: Acrylic resin, Kelakai leaf extract paste, Surface roughness. ABSTRAKLatar Belakang: Kehilangan gigi berdasarkan Riskesdas pada usia 35-44 tahun sebesar 17,5% dan meningkat pada usia 65 tahun ke atas (30,6%). Gigi tiruan digunakan untuk memperbaiki fungsi fisik, psikologis dan sosial penderita kehilangan gigi. Membersihkan gigi tiruan merupakan aspek penting dalam perawatan gigi tiruan untuk menjaga kesehatan mulut. Pasta ekstrak daun kelakai dapat menjadi pasta pembersih gigi tiruan alternatif karena bahan abrasifnya rendah dan juga ekstrak daun kelakai (Stenochlaena palustris) pada konsentrasi 30% memiliki rata-rata zona hambat 16.60 mm terhadap Candida albicans. Tujuan: Mengetahui pengaruh pasta ekstrak Kelakai (Stenochlaena palustris) konsentrasi 30% terhadap kekasaran permukaan heat cured acrylic. Metode: True experimental dengan rancangan pretest and posttest with control group design. Penelitian menggunakan 27 sampel terbagi menjadi 3 kelompok perlakuan dengan 2 kali pengukuran. Kelompok 1 adalah kontrol negatif, kelompok 2 adalah kontrol positif menggunakan pasta gigi dan kelompok 3 adalah kelompok perlakuan pasta ekstrak. Hasil: Hasil analisis uji Post Hoc Mann Whitney menunjukkan terdapat perbedaan signifikan pada pasta ekstrak kelakai konsentrasi 30% dengan kelompok kontrol. Hasil penelitian ini juga didapatkan nilai rata-rata kekasaran permukaan setelah ppemberian pasta ekstrak sebesar 0,090 µm. Kesimpulan: Terdapat pengaruh pasta ekstrak Kelakai konsentrasi 30% terhadap kekasaran permukaan heat cured acrylic. Kata kunci: Kekasaran permukaan, Pasta ekstrak daun kelakai, Resin akrilik

PENGARUH LARUTAN ASAM LAKTAT DAN SALIVA BUATAN TERHADAP ION FLUOR WATER SETTABLE GIC Yudha Gilang Saputra; Isyana Erlita; Agung Satria Wardhana
Dentin Vol 6, No 3 (2022)
Publisher : Universitas Lambung Mangkurat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/dentin.v6i3.6821

ABSTRACTBackground: Secondary caries is a caries lesion that starts at the margin of the restoration, it is influenced by the salivary flow rate. Conventional GIC materials are easily soluble in salivary flow so thar Water Settable GIC is a solution for dentist in terms of practicality and strength as the latest restorative material. Objectives: To analyze the effect of lactic acid and artificial saliva on the releasw of fluoride ion Water Settable GIC. Methods: Forty-eight specimens (diameter 5 mm x thickness 2 mm; n = 8 / group) were made with Water Settable GIC. Samples that met the criteria were divided into 6 groups. Samples were immersed for 1 and 7 days in an incubator in 37oC temperature. For control group aquadest used to immerse the samples. The levels of fluoride ion release were read using a UV-VIS spectrophotometer. The data obtained were tested using the SPSS application. Results: The One-Way Anova parametric test results of day one and seven (p<0,05). The Bonferonni Post Hoc test showed that there was a significant difference between all treatment groups lactic acid day 1 (10,505 ± 0,285), lactic acid day 7 (11,609 ± 0,202), artificial saliva day 1 (4,639 ± 0,227), artificial saliva day 7 (8,273 ± 0,184), aquadest day 1 (3,286 ± 0,475), aquades day 7 (5,778 ± 0,823). Conclusion: There is an effect of lactic acid and artificial saliva on fluoride ion from Water Settable GIC. Keyword: Aquadest, Artificial saliva, Lactic acid, Release of ion fluoride, Water Settable GIC. ABSTRAK Latar Belakang: Karies sekunder adalah lesi karies yang dimulai dari tepi restorasi. Hal ini dipengaruhi oleh kecepatan aliran saliva. Bahan GIC konvensional mudah larut dalam aliran saliva, sehingga Water Settable GIC menjadi solusi bagi dokter gigi dalam hal kepraktisan dan kekuatan sebagai bahan restorasi terbaru. Tujuan: Menganalisis pengaruh larutan asam laktat dan saliva buatan terhadap jumlah pelepasan ion fluor Water Settable GIC Metode: Empat puluh delapan spesimen (diameter 5 mm x ketebalan 2 mm; n= 8/kelompok dibuat dengan Water Settable GIC. Sampel yang sudah sesuai kriteria dibagi menjadi 6 kelompok. Sampel direndam selama 1 dan 7 hari didalam inkubator 37oC. Jumlah pelepasan ion fluor dibaca menggunakan alat spektrofotometer UV-VIS.Hasil: One-Way Anova hari ke-1 dan 7 menunjukkan bahwa (p<0,05) dan uji Post Hoc Bonferonni menunjukkan terdapat perbedaan yang bermakna antara semua kelompok perlakuan asam laktat hari ke-1 (10,505 ± 0,285), asam laktat hari ke-7 (11,609 ± 0,202), saliva buatan hari ke-1 (4,639 ± 0,227), saliva buatan hari ke-7 (8,723 ± 0,184), akuades hari ke-1 (3,286 ± 0,475), akuades hari ke-7 (5,778 ± 0,823). Kesimpulan: Terdapat pengaruh asam laktat dan saliva buatan terhadap jumlah pelepasan ion fluor Water Settable GIC. Kata kunci: Akuades, Asam laktat, Jumlah pelepasan ion fluor, Saliva buatan, Water Settable GIC.

THE EFFECT OF IMMERSION IN 50% CONCENTRATION OF KASTURI LEAF EXTRACT (MANGIFERA CASTURI) ON THE DISCOLORATION OF HEAT CURED ACRYLIC RESIN Agung Satria Wardhana; Mikael Manggala Silaen; Rahmad Arifin
Dentino : Jurnal Kedokteran Gigi Vol 8, No 1 (2023)
Publisher : FKG Unlam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/dentino.v8i1.16082

ABSTRACTBackground: Heat cured acrylic resin and alkaline proxide are the most used material for denture base and denture cleaner. However, the cost of denture cleanser and its discoloration effect after long term use became the major disadvantages of this material. Kasturi leaf extract, in 50% concentration, had been proven to have antibacterial and antifungal effect, such as a denture cleanser properties.. Objective: This study was to determine the discoloration effect of immersing heat cured resin acrylic in to 50% Kasturi leaf extract solution compared to alkaline peroxide and distilled water. Material and Methods: This study used 24 samples of heat cured acrylic following ADA specification no 17, with 15 milimeters diameter and 2 millimeters of thickness. The 50% Kasturi leaf extract treatment group and the control group alkaline peroxide and aquades. Each group consisted of 8 samples which measured the discoloration before and after being immersed for 5 days. The discoloration of the sample was tested using a series of digital analysis tools. The results obtained then converted into the equation of ΔE= [(ΔL)2 + (Δa)2 + (Δb)2]1/2. Results: One Way ANOVA and Post Hoct Bonferroni statistical tests showed that there was a significant difference (p <0.05) between the immersion groups in 50% Kasturi leaf extract (6.75 ± 1.31), alkaline peroxide (3.29 ± 0, 57) and distilled water (2.04 ± 0.60). Conclusion: A 50% solution of Kasturi leaf extract has an effect on the discoloration of heat cured type acrylic resin. Keywords: Discoloration, Heat cured acrylic resin, Kasturi leaves

DIFFERENCES OF BIOACTIVE COMPOSITE RESIN COLOR ON IMMERSION IN ALCOHOL AND NON-ALCOHOL MOUTHWASH Rosyaningsi Rosyaningsi; Isyana Erlita; Agung Satria Wardhana
Dentin Vol 7, No 2 (2023)
Publisher : Universitas Lambung Mangkurat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/dentin.v7i2.9719

Background: Composite resin is the most used material for caries restoration and highly chosen by dental patients regarding it’s high aesthetic value. People often use mouthwash to maintain dental and oral hygiene, both those containing alcohol and non-alcohol. According to several studies, the alcohol in mouthwash will cause a decrease in the physical properties of the composite resin, such as surface roughness, compressive strength, hardness, and also the aesthetic value of the composite resin. Objective: To determine the difference of bioactive composite resin color on immersion in alcohol and non alcohol mouthwash. Methods: Experimental laboratory with a pre-test and post-test with control group design using 27 samples of composite resin with a diameter of 15 mm and 1 mm. The samples were divided into 3 groups, namely the immersion group in alcoholic mouthwash, non-alcohol, and aquades. Each group consisted of 9 samples to be measured the value of color change before and after immersion for 12 hours. Results: The results of the One Way ANOVA statistical test showed that there was an effect of soaking mouthwash on the color of the bioactive composite resin. The Post Hoc LSD test showed between the alcohol and non-alcohol mouthwash group, as well as alcohol and distilled water group had a significant difference, but not in the non-alcohol mouthwash and distilled water group. Conclusion: The immersion in both alcohol and non- alcohol mouthwash are significantly affecting the color changing in bioactive composite resin. The value of color changing between mouthwash goups are significantly different. Keywords: Bioactive composite resin, discoloration, liquid absorption, mouthwash.

THE RELATIONSHIP OF TOOTH BRUSHING BEHAVIOR, DIETARY PATTERNS, ECONOMIC STATUS ON CARIES OF SOUTH DAHA POPULATION Maulida Arifa Yasmin; Raden Harry Dharmawan Setyawardhana; Agung Satria Wardhana
Dentin Vol 7, No 2 (2023)
Publisher : Universitas Lambung Mangkurat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/dentin.v7i2.9720

Background: Based on Riskesdas 2018, prevalence of caries between age of 55-64 is 96,8%. The DMT-T index in Samuda Village, South Daha is 8,2. Caries is the destruction of hard surface of the teeth caused by multifactorial etiology, such as host, substrate, time, microorganisms and predisposing factors like tooth brushing behavior, dietary patterns and economic status. Objectives: Analyzed the relationship between tooth brushing behavior, dietary patterns, economic status and dental caries of South Daha population. Methods: This study was an observational analytic research with cross sectional research design. The population was 2,559 people of South Daha aged between 55-64 and 108 people was involved in this study based on simple random sampling technique. Results: Correlation test result, using Somers'd test to analyze the relationship between tooth brushing behavior and dental caries, is 0.000 (p <0.050), which means there is a relationship between tooth brushing behavior and dental caries with correlation strength is 0.582 (moderate). The correlation result between dietary patterns and dental caries is 0.000 (p <0.050) with correlation strength is 0.239 (weak), which means there is a weak relationship between dietary patterns and dental caries. The correlation between economic status and dental caries is 0.000 (p <0.050) with correlation strength is 0.353 (weak), which means there is a weak relationship between economic status and caries. Conclusion: There is a relationship between tooth brushing behavior and dental caries, there is a relationship between dietary patterns and dental caries and there is a relationship between economic status and dental caries. Keywords: Caries, dietary patterns, economic status, tooth brushing behavior,

EFEKTIVITAS ANTIBAKTERI EKSTRAK DAUN KECAPI (Sandoricum koetjape Merr) TERHADAP PERTUMBUHAN BAKTERI Enterococcus faecalis Yusrinie Wasiaturrahmah; Muhammad Rayhan; Deby Kania Tri Putri; Agung Satria Wardhana; Maharani Laillyza Apriasari
Jurnal Insan Farmasi Indonesia 2023: JIFI: Webinar & call for paper
Publisher : Sekolah Tinggi Ilmu Kesehatan ISFI Banjarmasin

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36387/jifi.v6i3.1699

Penyebab terbesar kegagalan perawatan saluran akar sebesar 63% disebabkan oleh bakteri Enterococcus faecalis. Bakteri Enterococcus faecalis dapat dieliminasi dengan melakukan irigasi saluran akar. Kandungan zat aktif seperti saponin yang terdapat di dalam daun kecapi (Sandoricum koetjape Merr) terbukti memiliki efek antibakteri. Tujuan mengukur dan menganalisis efektivitas antibakteri ekstrak daun kecapi (Sandoricum koetjape Merr) terhadap pertumbuhan bakteri Enterococcus faecalis. Metode penelitian ini merupakan eksperimen murni (true experimental) dengan post test only with control group design. Penelitian ini terdiri dari 9 kelompok perlakuan, yaitu ekstrak daun kecapi (Sandoricum koetjape Merr) konsentrasi 10%, 20%, 30%, 40%, 50%, 60%, 70%, sodium hipoklorit (NaOCl), dan aquadest dengan 3 kali pengulangan. Data KHM didapatkan dari pengukuran delta Optical Density (OD) dan data uji KBM didapatkan berdasarkan perhitungan jumlah koloni. Berdasarkan nilai rata-rata dan analisis data diketahui KHM terdapat pada konsentrasi ekstrak daun kecapi 10% dan KBM pada konsentrasi 20%. Ekstrak daun kecapi (Sandoricum koetjape Merr) konsentrasi 10%, 20%, 30%, 40%, 50%, 60%, dan 70% memiliki efektivitas antibakteri terhadap pertumbuhan bakteri Enterococcus faecalis.

Efficacy of Hyperbaric Oxygen Therapy in Systemic Lupus Erythematosus Patients Undergoing Maxillofacial Surgery Eko Mukti Wibowo; Ganendra Anugraha; Agung Satria Wardhana
Majalah Biomorfologi Vol. 35 No. 1 (2025): MAJALAH BIOMORFOLOGI
Publisher : Universitas Airlangga, Surabaya, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/mbiom.v35i1.2025.69-80

Highlights SLE is the most common autoimmune disease that requires management after maxillofacial surgery. Hyperbaric oxygen therapy is a treatment that can help accelerate recovery in patients. Abstract Background: Systemic Lupus Erythematosus (SLE) is a common multifactorial autoimmune disease that carries a high risk of osteoporosis and fractures. The delivery of oxygen to the body's tissues through hyperbaric oxygen treatment (HBOT) promotes the healing of wounds and shortens the typical recovery time for patients. The way tissues react to illnesses and injuries can be altered by HBOT. Objective: This article aimed to determine the use of hyperbaric oxygen therapy (HBOT) post-maxillofacial surgery in SLE patients. Materials and Method: This research used a scoping review approach, accessing electronic databases like PubMed, Scopus, Science Direct, Elsevier, and Google Scholar. Discussion: SLE is characterized by producing various autoantibodies that interact with endogenous antigens, favoring widespread inflammatory injury. SLE impacts the immune system, diminishing its ability to defend against infections. Hyperbaric oxygen therapy refers to administering 100% oxygen to a patient inside a pressurized chamber with a pressure higher than one atmosphere at sea level. The surgical placement of the implant into the mandible or maxilla allows it to be retained during functional loading, as the bone integrates with the implant during growth. Conclusion: HBOT can improve the host response by activating inflammatory cells and ensuring optimal oxygen tension in people with SLE. This can help with osteogenesis and neovascularization, which fill empty spaces with new blood vessels or bone tissue.

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