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Gambaran histopatologi penyembuhkan luka pencabutan gigi pada makrofag dan neovaskular dengan pemberian getah batang pisang ambon Hendrik Setia Budi; Pratiwi Soesilowati; Zhafirah Imanina
Majalah Kedokteran Gigi Indonesia Vol 3, No 3 (2017): December
Publisher : Faculty of Dentistry, Universitas Gadjah Mada

Tooth extraction healing on macrophages and neovascular histopathology induced by ambonese banana stem sap gel. Blood clotting is very important in the early wound healing process. Blood clots will soon be filled with a connective tissue due to the induction of growth factors as granulation tissue. The expression of platelet derived growth factors (PDGF)-BB using the Ambonese banana stem sap gel (Musa paradisiaca var. sapientum) can increase the proliferation of fibroblasts. Macrophages and neovascular support in the early phase of healing. The purpose of this research was to demonstrate that Ambonese banana stem sap gel (GEGPA) can improve the macrophages and neovascular in the socket wound healing. Thirty six wistar rats were used and divided into 3 groups. The mandibular left incisor was extracted. The first group as control was given HPMC 4% gel on socket, the second group was given GEGPA 60%, and third group was given gelatin sponge. Rats were sacrificed on day 3 and 5 for histopathology examination of neovaskular and macrophages on the socket. The results showed that there was significantly different in the control group on a number of neovascular and macrophages in socket on day 3 (p<0.05). No significant difference on day 5 in all groups of neovascular and macrophages number (p>0.05). It was concluded that the use of GEGPA 60% acceleratedthe wound healing of tooth extraction socket by means of macrophages and neovascular increasing.ABSTRAKPembekuan darah sangat penting pada proses awal penyembuhan luka. Bekuan darah akan segera diisi oleh jaringan granulasi yang berasal dari induksi faktor pertumbuhan. Ekspresi platelet derived growth factors (PDGF)-BB yang diinduksi oleh pemberian gel ekstrak getah batang pisang ambon (Musa paradisiaca var. sapientum) dapat menunjukkan terjadinya peningkatan proliferasi fibroblas. Makrofag dan neovaskular sangat menunjang fase awal penyembuhan. Tujuan penelitian ini untuk membuktikan bahwa pemberian gel ekstrak getah batang pisang ambon (GEGPA) dapat meningkatkan jumlah makrofag dan neovaskular pada penyembuhan luka soket. Tikus strain Wistar sebanyak 36 ekor digunakan pada penelitian ini yang terbagi menjadi 3 kelompok. Semua tikus dilakukan pencabutan gigi pada insisive kirimandibula. Kelompok pertama sebagai kontrol, pada soket diberi gel hidroksipropil metil selulosa (HPMC) 4%, kelompok kedua diberi GEGPA 60%, dan kelompok ketiga diberi gelatin sponge. Tikus dikorbankan pada hari ke-3 dan 5 untuk pemeriksaan histopatologi neovaskular dan makrofag pada soket. Hasil penelitian menunjukkan terdapat perbedaan yang bermakna terhadap kelompok kontrol pada jumlah neovaskular dan makrofag soket pada hari ke-3 (p<0,05). Tidak ditemukan perbedaan yang bermakna pada jumlah neovaskular dan makrofag hari ke-5 (p>0,05). Disimpulkan bahwa pemberian GEGPA 60% dapat mempercepat penyembukan luka pencabutan gigi melalui peningkatan jumlah makrofag dan neovaskular.

TOXICOLOGICAL SCREENING OF ELLAGIC ACID IN POMEGRANATE FRUIT AND HYDROXYAPATITE COMBINATION AS BONE GRAFT MATERIAL ON BHK-21 FIBROBLAST CELL Agung Satria Wardhana; Isyana Erlita; Intan Nirwana; Hendrik Setiabudi
Dentino : Jurnal Kedokteran Gigi Vol 5, No 1 (2020)
Publisher : FKG Unlam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/dentino.v5i1.8132

Background: Bone graft is an alternative therapy for periodontitis and other bone destructive lesions. Several studies had revealed Ellagic Acid (EA) ability in increasing osteogenesis process. EA contains polyphenols, such as Ellagitannin, Gallotannin, and Anthocyanin, which demonstrate anti-inflammatory and antibacterial activity as well as growth factor stimulating effect. EA combination with bone graft material (hydroxyapatite) is anticipated to enhance bone osteogenesis yet no investigation was performed to identify its toxicity towards fibroblast cell. Objective: To analyze EA toxicity on fibroblast cell in vitro. Methods: This was a true experimental study using post-test only with control group design. Fibroblast cell was exposed with EA in eight different concentrations: 0.1%, 0.2%, 0.5%, 1%, 2%, 3%, 4% and 5%. Control group comprised of cell control and media group. All groups were exposed to MTT Assay test and measured using Elisa Reader. Result: The calculation of cell viability value in EA groups at 0.1%, 0.2%, 0.5%, 1%, 2%, 3%, 4% and 5% concentration were 88.2%, 92.3%, 97.5%, 89.5%, 84.2%, 90.7%, 88.9% and 89.4% respectively. Conclusion: All EA and hydroxyapatite combinations are not toxic towards BHK-21 fibroblast cells.

Antioxidant activity test on ambonese banana stem sap (Musa parasidiaca var. sapientum) Hendrik Setia Budi; Indah Lisatiana Kriswandini; Aditya Dana Iswara
Dental Journal (Majalah Kedokteran Gigi) Vol. 48 No. 4 (2015): December 2015
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Background: Polymorphonuclear cells (PMN) release oxygen free radicals or reactive oxygen species (ROS) during inflammation. As a result, ROS level is higher than antioxidant level in our body during oxidative stress leading to prolong inflammation or continuous tissue damage. Indonesia, on the other hand, is a country with various herbal medicines. For instance, ambonese banana (Musa parasidiaca var. sapientum) is often used as herbal medicine. Ambonese banana, moreover, has flavonoid, polyphenol, tannin, and saponin as antioxidants to reduce free radicals by transferring their hydrogen atom. Medicine used to reduce the impact of free radicals is known as antioxidant. Antioxidant is proved to accelerate wound healing. Purpose: This research aims to analyze the effects of the antioxidant activity of Ambonese banana stem sap extract. Method: Antioxidant activities in this research were examined with 1,1-Diphenyl-2-picryl-hidrazyl (DPPH) method by reacting with stable radical compounds. Spectrophotometry with a wavelength of 517 nm was used to measure absorption results shown in purple. The absorption results then were calculated by IC50 reduction activity. Result: There were significant differences of Ambonese banana stem sap antioxidant activity (p<0.05) at the concentrations of 15%, 30%, and 60 %. All concentrations have greater absorbance scores than IC50 (>50%). Conclusion: Ambonese banana stem sap extract has antioxidant activities.

Antibacterial effects of 70% ethanol and water extract of cacao beans (Theobroma cacao L.) on Aggregatibacter actinomycetemcomitans Ayu Rafania Atikah; Hendrik Setia Budi; Tuti Kusumaningsih
Dental Journal (Majalah Kedokteran Gigi) Vol. 49 No. 2 (2016): June 2016
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Background: Aggregatibacter actinomycetemcomitans (A. actinomycetemcomitans) is a Gram negative bacteria that form a subgingival plaque causing periodontitis. Nowadays, many natural resources can be used as a basic ingredient of drugs. One of the resources used as an antibacterial material is cacao bean. It contains of polyphenol flavonoids, such as catechin, epicatechin, anthocyanin, and proanthocyanidin. Chemical compounds contained in ethanol extract and water extract are different in quantity from those in cocoa beans. Purpose: This research aimed to find out difference in antibacterial activity between the 70% ethanol and water extract of cacao beans (Theobroma cacao L.) on A. actinomycetemcomitans. Method: This research was an in vitro laboratory experiment. The serial dilutions was performed on the 70% ethanol and water extract of cacao beans a concentration of 100% to 3.125%. At each concentration, the 70% ethanol and water extract of cacao beans were added with grown bacterial suspension of A. actinomycetemcomitans. After they were incubated for 24 hours, the bacteria grown on Luria Berthani media were observed. Bacteria colonies then were measured in CFU/ml. Result: There were significant differences in bacterial colonies grown at the concentrations of 6.25% and 3.125% between the 70% ethanol extract of cacao beans and the water extract of cacao beans as p-value = 0.000 (p<0.05). Conclusion: 70% Ethanol beans and water extract of cacao beans have antibacterial activity against A. Actinomycetemcomitans. The concentrations of MIC and MBC extracts were 6.25% and 12.5% respectively.

Antibacterial ability of arabica (Coffea arabica) and robusta (Coffea canephora) coffee extract on Lactobacillus acidophilus Willy Wijaya; Rini Devijanti Ridwan; Hendrik Setia Budi
Dental Journal (Majalah Kedokteran Gigi) Vol. 49 No. 2 (2016): June 2016
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Background: Dental caries is the most commonly dental health problem found in Indonesia. Lactobacillus acidophilus (L. acidophilus) is bacteria playing a role in the development and continuation of caries. Some researches in Dentistry Faculty show that many plants are efficacious for oral health. One of them is coffee bean. Coffee bean containing caffeine, phenolic, trigonelline, and chlorogenic acid is reported to have antimicrobial activity. Purpose: This research aimed to determine the differences in the inhibition of Arabica and Robusta coffee extract to L. acidophilus. Method: This research was an laboratory experimental research. The method used was well diffusion method using seven samples for each treatment group. BHI-A and inoculated L.acidophilus bacteria was poured into each petri dish, and then 8 pitted holes were made with a diameter of 5mm and a depth of 3mm using a ring. Next, Arabica or Robusta coffee extracts at a concentration of 100%, 75%, 50%, 12.5%, 6.25%, and 3.125% were put into each of the pitted hole until it was full, and a negative control was also prepared. They then were put in an incubator at a temperature of 37 °C for 24 hours. Afterwards, measurements and observations were conducted on inhibition zone area. Result: Robusta coffee extract at the concentrations of 100% and 75% had greater inhibitory than Arabica coffee extract (p<0.05). Meanwhile, Arabica and Robusta coffee extracts at the concentrations of 50% and 25% had no significant inhibitory difference (p>0.05). Conclusion: In conclusion, Robusta and Arabica coffee extracts have inhibitory effects on L.acidophilus. Robusta coffee bean extract, nevertheless, has better inhibitory effects than Arabica coffee bean extract.

FGF-2 expression and the amount of fibroblast in the incised wounds of Rattus norvegicus rats induced with Mauli banana (Musa acuminata) stem extract Didit Aspriyanto; Intan Nirwana; Hendrik Setia Budi
Dental Journal (Majalah Kedokteran Gigi) Vol. 50 No. 3 (2017): September 2017
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Background: Traditional wound treatment using herbal medicine is thought to maintain the health of families and society in general economically, effectively, and efficiently without inducing side effects. One genus of plant that can be used as a traditional medicine is the Mauli banana, indigenous to South Borneo. Mauli banana stem contains bioactive compounds, most of which are tannins along with ascorbic acid, saponin, β-carotene, flavonoids, lycopene, alkaloids, and flavonoids. Tanin has antibacterial and antioxidant effects at low concentrations, as wells as antifungal ones at high concentrations. Purpose: This study aimed to analyze the effects of Mauli banana stem extract at concentrations of 25%, 37.5%, and 50% on the quality of incised wound healing in male Rattus norvegicus rats by assessing FGF-2 expression and fibroblast concentration on days 3 and 7. Methods: This research represented an experimental laboratory-based investigation involving 32 rats of the Rattus norvegicus strain aged 2-2.5 months old. Sampling was performed using a simple random sampling technique since the research population was considered homogeneous and divided into 8 treatment groups (C3, M3-25, M3-37.5, M3-50, C7, M7-25, M7-37.5, M7-50). The rats in each group were anesthetized before their back was incised with length and width of 15x15mm with a depth of 2mm. Gel hydroxy propyl cellulose medium (HPMC) was applied to the incised wound of each rat in the control group, while stem Mauli banana extract was applied to that of each rat in the treatment groups three times a day at an interval of 6-8 hours. On day 3, four rats from each group were sacrificed, while, in the remaining groups, the same procedure was performed until day 7, at which point they (8 groups) were sacrificed for HE examination in order to assess the amount of fibroblast and for IHC examination to examine FGF-2 expression. Data regarding FGF-2 expression and the amount of fibroblast were analysed by means of One-way Anova and HSD. Results: The results showed that the Mauli banana stem extract could significantly improve the expression of FGF-2 and the amount of fibroblast cells compared to C3 and C7 groups. The highest increase in FGF-2 expression and fibroblast amount were found in all groups on days 3 and 7 treated with the Mauli banana stem extract at a concentration of 50%. Conclusion: There was an increase of FGF-2 expression and the amount of fibroblast cells in the incision wound healing process that induced with Mauli banana stem extract.

Ujitoksisitasakut ekstrakbatangpisangambon(Musa paradisiacavar sapientum) terhadap hati mencit (Mus musculus) dengan parameter LD (Acute toxicity test of ambonese banana (Musa paradisiaca var sapientum) stem extract in liver of mice (Mus musculus) with LD 50 parameters 50 Hendrik Setia Budi; Ira Arundina; Retno Indrawati; Leonita Widyana Mahardikasari
Journal of Dentomaxillofacial Science Vol. 13 No. 2 (2014): Formerly Jurnal Dentofasial ISSN 1412-8926
Publisher : DiscoverSys Inc.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15562/jdmfs.v13i2.394

The sap of ambonese banana (Musa paradisiaca var sapientum) contain flavonoids, saponins, tannins which have beenwidely used by people in Trunyan Bali as traditional medicine on wound healing, and it has been reported as apotential wound healing after tooth extraction. The aim of this research was to determine the level of safety of usingherbal medicine, ambonese banana stem extract on histopatology liver of mice with LDparameters. This experimentalstudy was performed bythe post test only controlled group design. The sample were 28 mice (Mus musculus) randomlydivided into 4 groups. K group as control group was given aquadest. P1, P2, and P3, as treatment groups were givenambonese banana stem extract with dose 0.42g/20gbw, 2.1g/20gbw, 4.2g/20gbw. The extract was given per-oral withsonde on the first day. On day 3, the mice were terminated, and the livers were microscopically histopathological observed. The observed at 3th50 day, there were no deaths in every groups of mice (K, P1, P2, and P3) on the third day observation. Kruskal Wallis test showed there was not significant difference in histopathological appearance on liverof mice (p=0.771). It wasconcluded that the maximumsafety dose that can still be administered is 0.42g/20gbw followingthe LDparameter. Theacute toxicity test of ambonese banana stem extract did not show necrosis on liver but it showedthe highest simple degeneration than all groups which were given 0.42g/20gbw dose.

Mewujudkan Masyarakat Sadar Dental Record melalui Pencatatan Gigi Keluarga secara Mandiri di Trawas Mojokerto Aqsa Sjuhada Oki; Hendrik Setia Budi; Yuliati Yuliati; Oki Fadhila
Jurnal Abdi Masyarakat Indonesia Vol 3 No 5 (2023): JAMSI - September 2023
Publisher : CV Firmos

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.54082/jamsi.861

Terletak di tiga lempeng tektonik besar yang bertemu, yaitu lempeng Indo-Australia, lempeng Eurasia, dan lempeng Pasifik, Indonesia merupakan salah satu negara yang paling rawan bencana alam. Negara kita telah mengalami banyak bencana alam, beberapa di antaranya tercatat sebagai bencana alam yang paling merusak dengan kekuatan besar. Gigi merupakan bagian dari identitas seseorang yang dapat terekam dan teridentifikasi jika menjadi korban kecelakaan transportasi atau bencana alam karena gigi merupakan bagian dari tubuh manusia yang dapat bertahan hidup meskipun tubuhnya rusak akibat kebakaran atau pembusukan. Dengan adanya dental record atau rekam gigi, identifikasi seseorang dapat dilakukan jauh lebih cepat dan akurat. Oleh karena itu, setiap keluarga diharapkan memiliki rekam gigi sebagai identitas untuk identifikasi jika diperlukan. Tujuan program ini adalah melatih masyarakat agar mampu membuat dental record keluarga secara mandiri, yang dapat dipergunakan sebagai data ante-mortem. Kami memperkenalkan metode sederhana untuk membina masyarakat pedesaan di Kecamatan Trawas, Jawa Timur, dengan harapan mereka mampu merekam struktur rongga mulut keluarganya secara mandiri. Dengan menggunakan model gigi, 50 subyek belajar tentang anatomi gigi, perubahan struktur gigi, dan diproyeksikan ke lembaran odontogram sederhana. Kami juga mengadakan pretest dan post-test untuk melihat kemajuan setelah pelatihan. Setelah beberapa sesi pelatihan intensif, post-test menunjukkan peningkatan pengetahuan subyek yang ditunjukkan dengan peningkatan skor post-test sebesar 96,56%. Bagaimanapun juga, ini adalah proyek pendahuluan yang harus diikuti dengan pendampingan berkelanjutan.

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