Claim Missing Document
Check
Articles

Found 25 Documents
Search

TOXICITY TEST OF SMALL WHITE GINGER EXTRACT ON BHK-21 FIBROBLAST CELLS IN VITRO Robiansyah Robiansyah; Debby Saputera; Rahmad Arifin
Dentino : Jurnal Kedokteran Gigi Vol 5, No 1 (2020)
Publisher : FKG Unlam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/dentino.v5i1.8113

Abstract

Background: Denture stomatitis is inflammation of the oral mucosa which supporting the denture that caused by Candida albicans. Candida albicans contamination can be prevented by immersing dentures into denture cleanser solution. One of the alternative ingredients that can be used as denture cleanser is small white ginger rhizome (Zingiber officinale var. Amarum). Objective: This study aimed to analyze whether small white ginger extract (Zingiber officinale var. Amarum) was toxic to BHK-21 fibroblast cells using the MTT assay method. Method: This study was conducted in 7 groups. Five groups consisted of extracts of 30%, 40%, 50%, 60%, 70% and 2 control groups comprised of media control and cell control. Absorbance was read using ELISA reader and cell viability was calculated. Results: The percentage of living cells of all groups of small white ginger extract treatment was 100%. The parametric analysis of One Way Annova showed p = 0.498 (p> 0.05) Conclusion: Small white ginger extract (Zingiber officinale var. Amarum) is not toxic to BHK-21 fibroblast cells using the MTT Assay method because cell viability of all concentration groups is  ≥ 60%.
TOXICITY TEST OF BAY LEAF EXTRACT ON BHK-21 FIBROBLAST CELLS IN VITRO Chintya Dewi Styo Ningrum; Debby Saputera; Rahmad Arifin
Dentino : Jurnal Kedokteran Gigi Vol 4, No 2 (2019)
Publisher : FKG Unlam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/dentino.v4i2.7067

Abstract

Background: Denture stomatitis is an inflammatory reaction in the oral mucosa that support the denture. This lesion is caused by the fungus Candida albicans and can be avoided by always maintain the cleanliness of denture. Bay leaf (Syzygium polyanthum) has flavonoid as the highest content which has antifungal and antioxidant properties so that bay leaf can be used as alternative ingredient for denture cleanser. Toxicity test needs to be done to determine the safety of this material. Objective: to analyze the toxicity of bay leaf extract (Syzygium polyanthum) to BHK-21 fibroblast cells using the MTT assay method. Method: This study is a true experimental study, which using posttest-only with control group design. The group, treated with bay leaf extract, were 25%, 20%, 15%, 10% and 5% concentrations including 2 control groups which were media control and cell control. Absorbance was read using ELISA reader and cell viability was calculated. Results: The percentage of living cells in all groups which treated with bay leaf extract was 100%. The parametric analysis of One Way Annova showed that there was no significant difference. Conclusion: Bay leaf extract (Syzygium polyanthum) does not have any toxic effect to BHK-21 fibroblast cell using the MTT assay method because cell viability in all treatment groups was ≥ 60%.
REHABILITATION OF MASTICATION AND LACK OF SPACE TREATMENT IN NARROW EDENTULOUS WITH FIXED-FIXED BRIDGE Rahmad Arifin; Fitriana Caessar Pertiwi
Dentino : Jurnal Kedokteran Gigi Vol 6, No 2 (2021)
Publisher : FKG Unlam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/dentino.v6i2.12003

Abstract

Background: Loss of posterior teeth can lead to disharmony in the curvature of the jaw. Antagonistic teeth and surrounding teeth will migrate to the edentulous causing reduced mastication function and TMJ disturbance. Several cases of narrow edentulous occurred due to tooth shifting caused by edentulous that was left empty for a long period. Creating a fixed-fixed bridge is aimed to restore the aesthetics, function, and comfort for patients which caused by the missing teeth. Fixed-fixed bridge consist of a retainer that function as a support to the abutment; a pontic that function to replace the missing tooth; and a connector that function to connect the pontic and the retainer. A narrow space for pontics can disrupt the aesthetic and functional aspects. Case Report: A 24 year-old male patient was referred to Prosthodontic Clinic, Gusti Hasan Aman Dental Hospital with a complain of pain on the jaw joint and missing of left lower molar for about 5 years. Patient always masticate on one side after the left lower molar was missing. Extraoral examination found the patient to be in good condition, no enlargement of major salivary glands, the vital signs were good, but upon TMJ examination there was clicking sounds on the left side. Intraoral examination showed space narrowing resulted from mesial and distal drifting of 37 and 35 but not significant, therefore it was still possible to perform rehabilitation using fixed-fixed bridge with abutments on 37 and 35 with modification of pontic shape for 36 to resemble a premolar tooth with occlusal surface resembling a molar tooth. Treatment planning for fixed prosthetic using fixed-fixed bridge with porcelain fused to metal material on 35, 36, and 37 with ridge lap pontic type modification Conclusions: Treatment was successful to provide enough space for the pontic by modification of the shape and the size of the pontic. Keywords: Fixed-fixed bridge. lack of space, mastication.
THE EFFECT OF IRONWOOD STEM BARK EXTRACT (Eusideroxylon zwageri) ON THE GROWTH OF Streptococcus mutans ON ACRYLIC RESIN DENTURE PLATE Amalia Noviyanti; I Wayan Arya Krishnawan Firdaus; Rahmad Arifin
Dentino : Jurnal Kedokteran Gigi Vol 6, No 1 (2021)
Publisher : FKG Unlam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/dentino.v6i1.10634

Abstract

ABSTRACTBackground: Streptococcus mutans is plaque-forming initiator bacteria. Plaque on the surface base of denture can cause color changing, bad breath, inflammation, and infection  called denture stomatitis. Denture hygiene must be maintained by denture soaking in 0.2% chlorhexidine gluconate. However, 0.2% chlorhexidine gluconatethat used incessantly can cause side effects such as the changing of denture color and the fading of denture base pigmentation. The ironwood stem bark extract can be an alternative for denture cleanser material. Objectives: To analyze the effect of ironwood stem bark extract on the growth of Streptococcus mutans on heat cured type acrylic resin denture plate. Methods: True experimental laboratories research was used with post test only control group design using 9 treatment groups which are 5%, 10%, 20%, 40%, 60%, 80%, 100% concentration of ironwood stem bark extract, 0.2% chlorhexidine gluconate and aquadest. The treatment was performed with 3 times repetition resulting in 27 total samples. Antibacterial activity was measured by calculating the bacteria colony number. Results: The 5% and 10% concentration of ironwood stem bark extract were effective in reducing Streptococcus mutans with30.3 CFU/ml and 10.3 CFU/ml average colony number. The ironwood stem bark extracts with 20%, 40%, 60%, 80%, 100% concentration are effective and equivalent to 0.2% chlorhexidine gluconate against Streptococcus mutans with 0 CFU/ml average value. Conclusion: The 20%, 40%, 60%, 80%, 100% concentration of ironwood stem bark extract is equally effective as 0.2% chlorhexidine gluconatein killing Streptococcus mutans,exhibiting potential to be an alternative for denture cleanser material.Keyword: 0.2% Chlorhexidine gluconate, ironwood stem bark extract, acrylic resin denture plate heat cured type, Streptococcus mutans.
THE SOAKING EFFECT OF 100% SMALL WHITE GINGER EXTRACT ON THE VALUE OF THE FLEXURAL STRENGTH OF ACRYLIC BASE (Flexural Strength Value of Acrylic Base Using Acrylic Resin Heat Cured Type) Nadya Islami; Debby Saputera; Rahmad Arifin
Dentino : Jurnal Kedokteran Gigi Vol 5, No 1 (2020)
Publisher : FKG Unlam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/dentino.v5i1.8114

Abstract

Background: The denture base material that is widely used is heat cured acrylic resin. One of mechanical properties of acrylic resin that must be considered in the selection of the basic denture material is flexural strength. Flexural strength is needed, among others, to resist the chewing power that must be received by dentures. The commonly used denture cleanser is Alkaline peroxide. 100% small white ginger extract can be used as natural denture cleanser. Objective:  This study aimed to compare the flexural strength of heat cured acrylic base which in soaked 100% small white ginger extract with Alkaline peroxide solution as artificial denture cleanser. Method: This study was a pure laboratory experimental study with post test only with control group design, using simple random sampling. The sample was rectangular with a size of 65x10x2,5 mm. The number of samples used was 24 heat cured resin acrylic which was divided into 3 immersion groups, 100% small ginger extract, Alkaline peroxide, and aquades. The immersion carried out for 3 days 19 hours 25 minutes, obtained from 5 minutes denture cleanser presentation every day for 3 years. Flexural strength was tested using a Universal Testing Machine 3 Point Bending. Result: The everage flexural strength value of heat cured acrylic resin after soaked in 100% small white ginger extract was 70,98, in Alkaline peroxide 87,37, and 91,05 in aquades. The data was annalyzed using parametric One Way ANOVA test and Post Hoc Bonferroni test. Conclusion: The flexural strength of heat cured acrylic resin that soaked in 100% small white ginger extract is smaller than Alkaline peroxide after immersed for 3 days 19 hours 25 minutes.
CORRELATION BETWEEN STRESS AND TEMPOROMANDIBULAR DISORDER IN ORPHANED ADOLESCENT IN BANJARMASIN Fellbyan Fellbyan; Rahmad Arifin; Galuh Dwinta Sari
Dentino : Jurnal Kedokteran Gigi Vol 5, No 2 (2020)
Publisher : FKG Unlam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/dentino.v5i2.8949

Abstract

ABSTRACTBackground: Temporomandibular disorder is a temporomandibular joint disturbance characterized with the pain in masticatory muscle and TMJ; clicking in TMJ; and deflection, deviation pattern with limitation in opening of the mouth. TMD can be found in adolescents with prevalence and severity increased along with the age. TMD in adolescent was caused by the increased of the masticatory muscle activity. In adolescent, it was caused by the increase of the emotional state especially stress. Stress is a condition that often experienced by every people including orphaned adolescent, who has more problems than the other adolescent. Purpose: The aim of this study was to analyze the correlation between stress and temporomandibular disorder in orphaned adolescent in Banjarmasin. Method: This study is an analytical observational research with cross sectional design. The study was involving 13-18 years old orphaned adolescents in Banjarmasin with purposive sampling. This study is using PSS for stress examination and RDC/TMD for TMD examination. The correlation between stress and TMD were analyzed with Spearman analysis test. Results: This research showed that 61% of the subject had moderate stress level and TMD was found in 68.3% of the subject, which consists of 58.5% had myofascial pain and 9.8% had disc displacement. Spearman analysis test showed that there is statistical correlation between stress and temporomandibular disorder in orphaned adolescent in Banjarmasin (p<0.05). Conclusion: There is a correlation between stress and temporomandibular disorder in orphaned adolescent in Banjarmasin. Keywords: Myofascial Pain, Stress, Temporomandibular disorder (TMD).
THE EFFECT OF SPRAYING MAULI BANANA (Musa acuminata) STEM EXTRACT 25% CONCENTRATION ON THE DIMENSIONAL STABILITY OF ALGINATE IMPRESSION Syaiful Haq Prasetya; Rahmad Arifin; Riky Hamdani
Dentino : Jurnal Kedokteran Gigi Vol 7, No 1 (2022)
Publisher : FKG Unlam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/dentino.v7i1.13099

Abstract

Background: Alginate is an ingredient derived from brown seaweed extract which is anhydro-P-d-mannuronic acid. Alginate is often used as an impression material. A factor that must be considered when using impression materials is to prevent the infection. Prevention of infection transmission is done by administering disinfectants by spraying. However, this material has imbibition properties and expansion can occur in the model. 25% Mauli banana extract solution can be used as a disinfectant in alginate impressions and does not affect the dimensional stability of the alginate impressions. Objective: To analyze the effect of spraying mauli banana (Musa acuminata) stem extract 25% concentration on the dimensional stability of alginat impression. Methods: This research was pure experimental research using pretest-posttest and control group design. The research sample consisted of 15 samples that divided into 3 groups; aquades, 1% sodium hypochlorite, and 25% Mauli banana stem extract. The research data were analyzed using One Way Anova. Results: The average value calculation of the difference before and after spraying the aquades was 0.504  mm, the 1% sodium hypochlorite group was 0.502 mm and the 25% Mauli banana stem extract was 0.328 mm. Statistical tests showed no significant differences among all treatment groups on alginate dimensional stability or there was no effect on spraying 25% Mauli banana stem extract in the dimensional stability of alginate impression with sig. 0,401 (p > 0.05). Conclusion: There was no significant effect on spraying Mauli banana (Musa acuminata) stem extract 25% on the dimensional stability of the alginate impression.
DESCRIPTION OF PARTIALLY EDENTULOUS PATTERN AMONG PATIENTS AT RSGMP GUSTI HASAN AMAN IN BANJARMASIN Huda Ainani; Rahmad Arifin; Ika Kusuma Wardani
Dentino : Jurnal Kedokteran Gigi Vol 6, No 1 (2021)
Publisher : FKG Unlam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/dentino.v6i1.10649

Abstract

ABSTRACTBackground: Partial edentulism is the process when single or multiple teeth is missing from the alveolar socket and it may become an indicator for oral health. Partial edentulism can occur since the age of six and the aging factor increases the risk. Partial edentulism can be caused by some factors including: ages, gender, economic level, education level, knowledge level, and the etiology of the tooth loss. Purpose: To describe the distribution of partially edentulous pattern from RSGMP Gusti Hasan Aman’s patients in Banjarmasin. Method:  This study was including patients from RSGMP Gusti Hasan Aman between the age of 17-45 years old in Banjarmasin using purposive sampling. Questionnaires were distributed to determined patient knowledge on oral health with Kennedy classification for the partially edentulous pattern. Result: Kennedy Class II was the most frequent edentulous pattern observed on the upper (37%) and the lower jaw (39%). In particular, the adolescent group had the highest percentage (43%) of partial edentulism based on age. Women had the highest percentage (57%) of partial eduntulism based on gender. Respondents with moderate economic status had the highest percentage (35%) of partial edentulism. Respondents that have high school or above education level were the highest percentage (87%) identified with partial edentulous.  The most common etiology that identified with partial edentulous was the caries (52%). Conclusion: It can be concluded that Kennedy class III is the most confronted partially edentulous pattern among patients at RSGMP Gusti Hasan Aman in Banjarmasin.Keywords: Partially Teeth Loss Pattern, Kennedy, RSGMP Gusti Hasan Aman
IN SILICO STUDY OF Stachytarpheta jamaicensis ACTIVE COMPOUNDS AS ANTIBACTERIAL MATERIAL Juliyatin Putri Utami; Sherli Diana; Rahmad Arifin
Dentino : Jurnal Kedokteran Gigi Vol 7, No 1 (2022)
Publisher : FKG Unlam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/dentino.v7i1.13105

Abstract

Background: Stachytarpheta jamaicensis is a wild plant from the Verbenanceae family that grows in tropical areas such as Indonesia. S. jamaecensis extract was proven to contain secondary metabolite compounds such as flavonoids, phenols, saponins, tannins and terpenoids. The active compound of S. jamaecensis can be used as a drug candidate in the medicine field, especially as an antibacterial compound. One of the first steps in predicting the effectiveness of these compounds can be done through in-Silico studies with molecular docking. Objective: This study aims to evaluate the interaction of active compound S. jamaecensis with bacterial proteins through an in silico study. Methods: Using in silico method with computational docking analysis on seven active compounds of S. jamaecensis namely apigenin, luteolin, chlorogenic acid, gamma butyric acid, dopamine, ipolamide and geraniol, as well as two antibacterial drugs (metronidazole and chlorhexidine) as comparisons bound with bacterial cell wall protein, namely Glucosamine-6-phosphate synthase (G1mS). Docking in silico uses Autodock Vina, which is integrated with PyRx 8.0 and visualized using Discovery Studio Visualizer v19.1.0.18287 (2019 version) with data presentation based on docking scores. Conclusion: The best binding affinity score has been the luteolin-G1mS complex with a binding affinity value of -10.8 kcal/mol and was the highest value compared to the comparison ligand binding and the binding of other active compounds of S. jamaecensis.
THE EFFECT OF ULIN (Eusideroxylon zwageri) STEM BARK EXTRACT ON THE GROWTH OF Candida albicans ON ACRYLIC RESIN DENTURE PLATES Annisa Fitri Yuniar; I Wayan Arya Krishnawan Firdaus; Rahmad Arifin
Dentino : Jurnal Kedokteran Gigi Vol 6, No 1 (2021)
Publisher : FKG Unlam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/dentino.v6i1.10637

Abstract

ABSTRACTBackground: Candida albicans is the main microorganism that causes denture stomatitis, thus denture soaking in cleansing solution is needed to protect them from Candida albicans contamination. The 0.2% Chlorhexidine gluconate is one of denture cleansers that induce side effects at prolonged use. An alternative ingredient that can be used as a denture cleanser is ulin stem bark extract. Objective: To determine the effect of ulin stem bark extract at 20%, 40%, 60%, 80%, 100% concentration on the growth of Candida albicans on acrylic resin denture plates. Method: True Experimental with post-test only with control group design was employed under 7 treatment groups consisting ulin stem bark extract at 20%, 40%, 60%, 80%, 100% concentration, 0.2% Chlorhexidine gluconate, and aquadest in a total of 28 samples. Acrylic resin samples that had been exposed to Candida albicans weresoaked in respective treatment for 15 minutes. Results: The average of Candida albicans colonies on acrylic resin denture plates after soaking in ulin bark extract at 20%, 40%, 60%, 80%, 100% concentration, 0.2% Chlorhexidine gluconate, and aquadest were 29.5 CFU/ml, 13.0 CFU/ml, 0 CFU/ml, 0 CFU/ml, 0 CFU/ml, 0 CFU/ml, and 155 CFU/ml. Based on Mann Whitney test, there was no significant difference when ulin stem bark extract at 60% concentration was compared to 0.2% Chlorhexidine gluoconate. Conclusion: Ulin stem bark extract at 20%, 40%, 60%, 80%, and 100% concentration have been proven to reduce Candida albicans colonies on acrylic resin denture plates, and the 60% concentration is equivalent to 0.2% Chlorhexidine gluconate.Keywords: Candida albicans,Eusideroxylon zwageri, Heat cured acrylic resin. Ulin stem bark extract.