Garuda - Garba Rujukan Digital

TOXICOLOGICAL SCREENING OF ELLAGIC ACID IN POMEGRANATE FRUIT AND HYDROXYAPATITE COMBINATION AS BONE GRAFT MATERIAL ON BHK-21 FIBROBLAST CELL Agung Satria Wardhana; Isyana Erlita; Intan Nirwana; Hendrik Setiabudi
Dentino : Jurnal Kedokteran Gigi Vol 5, No 1 (2020)
Publisher : FKG Unlam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/dentino.v5i1.8132

Background: Bone graft is an alternative therapy for periodontitis and other bone destructive lesions. Several studies had revealed Ellagic Acid (EA) ability in increasing osteogenesis process. EA contains polyphenols, such as Ellagitannin, Gallotannin, and Anthocyanin, which demonstrate anti-inflammatory and antibacterial activity as well as growth factor stimulating effect. EA combination with bone graft material (hydroxyapatite) is anticipated to enhance bone osteogenesis yet no investigation was performed to identify its toxicity towards fibroblast cell. Objective: To analyze EA toxicity on fibroblast cell in vitro. Methods: This was a true experimental study using post-test only with control group design. Fibroblast cell was exposed with EA in eight different concentrations: 0.1%, 0.2%, 0.5%, 1%, 2%, 3%, 4% and 5%. Control group comprised of cell control and media group. All groups were exposed to MTT Assay test and measured using Elisa Reader. Result: The calculation of cell viability value in EA groups at 0.1%, 0.2%, 0.5%, 1%, 2%, 3%, 4% and 5% concentration were 88.2%, 92.3%, 97.5%, 89.5%, 84.2%, 90.7%, 88.9% and 89.4% respectively. Conclusion: All EA and hydroxyapatite combinations are not toxic towards BHK-21 fibroblast cells.

Sitotoksisitas resin akrilik hybrid setelah penambahan glass fiber dengan metode berbeda (Cytotoxicity of the hybrid acrylic resin after glass fiber reinforcement with difference method) Intan Nirwana; R. Helal Soekartono
Dental Journal (Majalah Kedokteran Gigi) Vol. 38 No. 2 (2005): June 2005
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Glass fiber reinforcement of the hybrid acrylic resin with difference method can enhance residual monomer content of the material; it can cause cytotoxic effect on fibroblast cells. The purpose of this study was to know the cytotoxicity of hybrid acrylic resins after glass fiber reinforcement with difference method on the cultured fibroblasts. The squared specimens of 10 mm in length, 10 mm in width and 1.5 mm in thickness were cured for 20 minutes at 100° C. The fibroblast cells were grown in Eagle's Minimum Essential Medium to be 2 × 105 cells/ml, then the cells were added to the samples in the plates and incubated at 37° C. After 48 hours, the cytotoxic effect was determined by direct cell number count using microscope and a hemocytometer. The statistical analyses using one way ANOVA and LSD test showed that there were significant difference in cell viability (p < 0.05) among the groups. The means percentage of cell viability were 90.00%, 99.,11%, 98.66%, it could be concluded that glass fiber reinforcement into hybrid acrylic resin with either first method or second method was not toxic.

Perlekatan koloni Streptococcus mutans pada permukaan resin komposit sinar tampak (The adherence of Streptococcus mutans colony to surface visible light composite resins) Ajeng Anggraeni; Anita Yuliati; Intan Nirwana
Dental Journal (Majalah Kedokteran Gigi) Vol. 38 No. 1 (2005): March 2005
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Visible light composite resins was used to restore anterior and posterior teeth, and it is always covered by saliva pellicle. S. mutans can adhere to all of the surface of oral cavity and visible light composite resins. The aim of this study was to know the amount of S. mutans colony adherence to visible light composite resins surface. The sample of 5 mm diameter and 3 mm in thickness was immersed in saliva for one hour, than the samples were put into bacteria suspension, incubated for 24 hours at 37° C. The amount of S. mutans was determined by direct count using microscope. The data were statistically analyzed by using t test. The result showed a significance difference of S. mutans colony between hybrid and micro fill visible light composite resins. The conclusion was that the amount of S. mutans adherence on the surface of hybrid was higher than the micro fill visible light composite resins.

Kekuatan transversa resin akrilik hybrid setelah penambahan glass fiber dengan metode berbeda (The transverse strength of the hybrid acrylic resin after glass fiber reinforcement with different method) Intan Nirwana
Dental Journal (Majalah Kedokteran Gigi) Vol. 38 No. 1 (2005): March 2005
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Different types of fibers have been added to acrylic resin materials to improve their mechanical properties. The purpose of this study was to know the transverse strength of the hybrid acrylic resins after glass fiber reinforcement with difference method. This study used rectangular specimens of 65 mm in length, 10 mm in width and 2.5 mm in thickness. There were 3 groups consisting of 6 specimens each, hybrid acrylic resin without glass fiber (control), glass fibers dipped in methyl methacrylate monomer for 15 minutes before being reinforced into hybrid acrylic resin (first method), glass fibers reinforced into a mixture of polymer powder and monomer liquid after the hybrid acrylic resin was mixed directly (second method). All of the specimens were cured for 20 minutes at 100° C. Transverse strength was measured using Autograph. The statistical analyses using one way ANOVA and LSD test showed that there were significant differences in transverse strength (p < 0.05) among the groups. The means of transverse strength were 94,94; 118,27; and 116,34 MPa. It meant that glass fibers reinforcement into hybrid acrylic resin enhanced their transverse strength compared with control. Glass fiber reinforcement into hybrid acrylic resin with differenciate method didn’t enhance their transverse strength.

The decrease of fibroblasts and fibroblast growth factor-2 expressions as a result of X-ray irradiation on the tooth extraction socket in Rattus novergicus Fatma Yasmin Mahdani; Intan Nirwana; Jenny Sunariani
Dental Journal (Majalah Kedokteran Gigi) Vol. 48 No. 2 (2015): June 2015
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Background: Wound healing involves cellular, molecular, physiological, and biochemical processes as responses to tissue damage. For instance, when a failure during tooth extraction occurs, radiographic examination, X-rays, is required. X-rays as an enforcer diagnosis can damage DNA chain, resulting in cell death and inhibition of wound healing process. Purpose: This research aims to analyze fibroblasts cell number and fibroblast growth factor-2 (FGF-2) expressions during wound healing process after tooth extraction as a result of X-ray irradiation. Methods: There were three research groups, each consisting of ten rats. Incisor tooth extraction was performed on the left lower jaw, and then X-ray examination was conducted at certain irradiation doses, namely 0 mSv, 0.08 mSv, and 0.16 mSv. Those animals were sacrificed on day 3, and on day 7 after the extraction, histopathology and immunohistochemistry examinations were conducted to determine fibroblast cell number and FGF-2 expressions. Data obtained were then analyzed by oneway ANOVA and Tukey HSD tests. Results: The number of fibroblasts decreased significantly in the group with the irradiation dose of 0.16 mSv applied on day 7 after the extraction (p <0.05). Similarly, the number of FGF-2 expressions decreased significantly in the group with the irradiation dose of 0.16 mSv applied on days 3 and 7 after the extraction (p <0.05). Conclusion: X-ray irradiation at a dose of 0.16 mSv can inhibit the healing process of tooth extraction wound due to the decreasing of fibroblasts cell number and FGF-2 expressions.

FGF-2 expression and the amount of fibroblast in the incised wounds of Rattus norvegicus rats induced with Mauli banana (Musa acuminata) stem extract Didit Aspriyanto; Intan Nirwana; Hendrik Setia Budi
Dental Journal (Majalah Kedokteran Gigi) Vol. 50 No. 3 (2017): September 2017
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Background: Traditional wound treatment using herbal medicine is thought to maintain the health of families and society in general economically, effectively, and efficiently without inducing side effects. One genus of plant that can be used as a traditional medicine is the Mauli banana, indigenous to South Borneo. Mauli banana stem contains bioactive compounds, most of which are tannins along with ascorbic acid, saponin, β-carotene, flavonoids, lycopene, alkaloids, and flavonoids. Tanin has antibacterial and antioxidant effects at low concentrations, as wells as antifungal ones at high concentrations. Purpose: This study aimed to analyze the effects of Mauli banana stem extract at concentrations of 25%, 37.5%, and 50% on the quality of incised wound healing in male Rattus norvegicus rats by assessing FGF-2 expression and fibroblast concentration on days 3 and 7. Methods: This research represented an experimental laboratory-based investigation involving 32 rats of the Rattus norvegicus strain aged 2-2.5 months old. Sampling was performed using a simple random sampling technique since the research population was considered homogeneous and divided into 8 treatment groups (C3, M3-25, M3-37.5, M3-50, C7, M7-25, M7-37.5, M7-50). The rats in each group were anesthetized before their back was incised with length and width of 15x15mm with a depth of 2mm. Gel hydroxy propyl cellulose medium (HPMC) was applied to the incised wound of each rat in the control group, while stem Mauli banana extract was applied to that of each rat in the treatment groups three times a day at an interval of 6-8 hours. On day 3, four rats from each group were sacrificed, while, in the remaining groups, the same procedure was performed until day 7, at which point they (8 groups) were sacrificed for HE examination in order to assess the amount of fibroblast and for IHC examination to examine FGF-2 expression. Data regarding FGF-2 expression and the amount of fibroblast were analysed by means of One-way Anova and HSD. Results: The results showed that the Mauli banana stem extract could significantly improve the expression of FGF-2 and the amount of fibroblast cells compared to C3 and C7 groups. The highest increase in FGF-2 expression and fibroblast amount were found in all groups on days 3 and 7 treated with the Mauli banana stem extract at a concentration of 50%. Conclusion: There was an increase of FGF-2 expression and the amount of fibroblast cells in the incision wound healing process that induced with Mauli banana stem extract.

Application of pomegranate (Punica granatum Linn.) fruit extract for accelerating post-tooth extraction wound healing Intan Nirwana
Dental Journal (Majalah Kedokteran Gigi) Vol. 51 No. 4 (2018): December 2018
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Background: Trauma occurring during tooth extraction can cause complications such as bleeding, infection, fracture and dry socket and constitutes an inflammatory response trigger. Pomegranate (Punica granatum Linn.) extract, which contains large amounts of punicallagin and ellagic acid, possesses various qualities, including; anti-inflammatory, anti-bacterial and anti-oxidant. Pomegranate extract can inhibit proinflammatory cytokine production, while also suppressing inflammation response thereby accelerating wound healing. Purpose: This study aimed to analyze the effect of pomegranate extract application to the tooth extraction wounds of Cavia cobaya (C. cobaya) on the expression of fibroblast growth factor-2 (FGF-2) and transforming growth factor β (TGF-β) on the fourth day of the wound-healing process. Methods: This study used 12 C. cobaya, divided into two groups, namely; control and treatment. The subjects were anesthetized, before their lower left central incisor was extracted and the entire socket filled with CMC-Na 3% in members of the control group and pomegranate extract in those of the treatment group. The twelve C. cobaya were sacrificed on day 4, their lower jaw subsequently being removed and decalcified for approximately 30 days. The mandibula tissue was stained using a immunohistochemical technique. FGF-2 and TGF-β were used to evaluate the healing process in the extracted tooth socket. Differences in the expression of FGF-2 and TGF-β were evaluated statistically by means of a t-test. Results: This study indicated a significant difference between the control and the treatment groups (p<0.05). The treatment group members whose sockets were filled with pomegranate extract showed high FGF-2 and TGF-β expression. Conclusion: This study confirmed that the administration of pomegranate extract to post-extraction tooth wounds of C. cobaya increases the expression of FGF-2 and TGF-β on day 4, thereby accelerating the wound healing process.

Validitas Lembar Kerja Peserta Didik (LKPD) pada Materi Pertidaksamaan Linier Dua Variabel Siti Hawa Siregar; Siti Ramadhani Putri Zulham; Nurul Intan Nirwana; Kamilah Silalahi; Ibrahim Yusup Nasution
JKPM (Jurnal Kajian Pendidikan Matematika) Vol 7, No 1 (2021): JKPM (Jurnal Kajian Pendidikan Matematika)
Publisher : Universitas Indraprasta PGRI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30998/jkpm.v7i1.11365

Penelitian ini bertujuan untuk mengembangkan lembar kerja peserta didik (LKPD) pada materi pertidaksamaan linier dua variabel yang layak untuk digunakan dalam kegiatan pembelajaran. Jenis penelitian ini adalah penelitian dan pengembangan atau Research and Development dengan menggunakan model Plomp. Model plomp ini terdiri atas lima tahapan dalam pelaksanaan pengembangannya, yaitu: 1. Tahap investigasi; 2. Tahap desain; 3. Tahap realisasi; 4. Tahap tes, evaluasi dan revisi; dan 5. Tahap implementasi. Data yang diperoleh kemudian dianalisis secara deskriptif kuantitatif dan juga kualitatif. Berdasarkan pada hasil penelitian dan pengembangan LKPD diperoleh hasil validasi 77,8 % pada validasi materi dengan kategori valid dan hasil 80% pada validasi media dengan kategori valid. Sehingga disimpulkan bahwa pengembangan LKPD pada materi pertidaksamaan linier dua variable yang dikembangan dengan menggunakan model Plomp valid untuk digunakan dalam kegiatan pembelajaran.

Proses Pengambilan Keputusan dalam Manajemen Ahmad Muktamar; Trisna Safitri; Intan Nirwana; Nurwahyudi Nurdin
Journal of International Multidisciplinary Research Vol. 2 No. 1 (2024): Januari 2024
Publisher : PT. Banjarese Pacific Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62504/t4z8gx04

Pengambilan keputusan manajemen yang akurat sangat penting bagi keberhasilan perusahaan di tengah seluk-beluk dan cepatnya perubahan lingkungan bisnis kontemporer. Proses pengambilan keputusan manajerial memerlukan langkah-langkah metodis yang bertujuan untuk menyelesaikan masalah dan mencapai tujuan organisasi. Penelitian ini bertujuan untuk mendalami seluk-beluk proses pengambilan keputusan dalam manajemen. Dengan menggunakan metodologi tinjauan literatur, khususnya metode deskriptif kualitatif dan penelitian perpustakaan, penelitian ini mengambil wawasan dari perpustakaan online dan platform akademis seperti Google Cendekia, Mendeley, DOAJ, serta literatur dan peneliti penelitian sebelumnya. Hasil temuan ini menyoroti pentingnya mempertimbangkan nuansa pengambilan keputusan, pengambilan keputusan manajerial, tahapan prosedural, gaya yang diadopsi, dan faktor-faktor yang mempengaruhi dalam proses pengambilan keputusan manajemen. Pengambilan keputusan yang efektif dan mewujudkan tujuan organisasi memerlukan penerapan model pengambilan keputusan yang sesuai, penggunaan teknik yang tepat, dan memperhitungkan potensi bias.

The Effect of Addition of Bambusa Arundinacea (Retz.) Willd. Fibers to Heat-Cured Acrylic Resin on Impact Strength Elonianty, Chanita; Agustantina, Titien Hary; nirwana, intan
Indonesian Journal of Dental Medicine Vol. 1 No. 2 (2018): Indonesian Journal of Dental Medicine
Publisher : Faculty of Dental Medicine Universitas Airlangga

Background: The prevalence of acrylic resin dentures fracture due to accidental drop is relatively high especially when it is being cleaned by patient. The effort to strengthen acrylic resin denture was done by adding Bambusa arundinacea (Retz.) Willd fibers, which was based on previous research. It has been proven that the addition of bamboo fiber to self-cured acrylic resin can increase the tensile strength. It has acceptable tensile properties, relatively low cost, high toughness, and good thermal properties. Purpose: To determine the impact strength of heat-cured acrylic resin after the addition of Bambusa arundinacea (Retz.) Willd. fibers at various lengths and weights. Methods: Bambusa arundinacea (Retz.) Willd. fibers with lengths of 2 mm, 6 mm, 12 mm and weights of 3wt%, 6wt%, 9wt% were added to heat-cured acrylic resin. The impact strength of heat-cured acrylic resin was tested using the Charpy method with mini impact tester KRY type. The test results were calculated using the impact strength formula. The research data were analyzed using one-way anova test and Tukey HSD test. Results: The data analysis showed that there was a significant difference in impact strength of heat-cured acrylic resin after the addition of Bambusa arundinacea (Retz.) Willd fibers. Conclusion: Addition of Bambusa arudinacea (Retz.) Willd. fibers increased the impact strength of heat-cured acrylic resin respective to the increment of length and weight of Bambusa arudinacea (Retz.) Willd. fibers.

Title

Found 11 Documents
Search

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Title Search

Found 11 Documents Search

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Title

Found 11 Documents
Search