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All Journal Dentino: Jurnal Kedokteran Gigi Dentin
I Wayan Arya K. Firdaus
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Dentistry

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COMPARISON OF INHIBITORY ACTIVITY OF KELAKAI LEAF EXTRACT AND 0.2% Chlorhexidine Gluconate AGAINST Streptococcus sanguinis ATCC® 10556™ Fitriana Caessar Pertiwi; I Wayan Arya K. Firdaus; Isyana Erlita
Dentino : Jurnal Kedokteran Gigi Vol 4, No 2 (2019)
Publisher : FKG Unlam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/dentino.v4i2.7066

Abstract

Background: Gingivitis is an early periodontal disease that occurs due to host response and dental plaque. Streptococcus sanguinis is pioneer of dental plaque colonization in oral cavity. Gingivitis treatment use 0,2% Chlorhexidine gluconate mouthwash, but long-term use causes side effects, so an alternative herbal mouthwash which has minimal side effects is needed. Kelakai leaf ethanol extract contains antibacterial compound, such as flavonoid, alkaloid, tannin and steroid, which can be herbal-based mouthwash as an alternative of 0,2% Chlorhexidine gluconate. Purpose: It was to compare the inhibitory zone of kelakai leaf ethanol extract and 0,2% Chlorhexidine gluconate against S. sanguinis. Materials and methods: This study was true experimental research and post-test only with control group design, that used 5 treatment groups with 4 replications of kelakai leaf ethanol extract, that were 25%, 50%, 75%, 100% concentrations and 0,2% Chlorhexidine gluconate against S.sanguinis with 20 total samples. All groups incubated for 24 hours at 37oC, then it measured the diameter of inhibition zone using calliper. Result: One Way ANOVA and Post Hoc LSD tests showed significant difference between all groups after given with kelakai leaf ethanol extract and 0,2% Chlorhexidine gluconate. Kelakai leaf ethanol extract 25%, 50%, 75%, 100% concentrations and 0,2%  Chlorhexidine gluconate has an average inhibition zone, which were 9,00 mm, 11,20 mm, 13,67 mm, 16,32 mm, and 18,12 mm against S.sanguinis. Conclusion: Kelakai leaf ethanol extract 25%, 50%, 75% and 100% concentrations has antibacterial activity against S.sanguinis, but not as strong as 0,2% Chlorhexidine gluconate.
EXFOLIATIVE CHEILITIS (Case report) I Wayan Arya K. Firdaus; Maharani Laillyza Apriasari
Dentino : Jurnal Kedokteran Gigi Vol 4, No 1 (2019)
Publisher : FKG Unlam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/dentino.v4i1.6169

Abstract

Background : Exfoliative Cheilitis is defined as a chronic inflammatory disorder of the vermillion border of the lips, which is characterized by the persistent formation of scales and crusts. Underlying stress or psychiatric conditions may cause or exacerbate exfoliative cheilitis. Purpose : This paper reports the mechanisme of stress as the predisposing factor of exfoliative cheilitis. Case : The patient had been suffering the desquamation, dryness, and tenderness of lips for three months. The disease was not treated by any drugs. She consumed the multivitamin then ate more fruits and vegetables, but it was not getting well. Case Management : The anamnesis results that she had an allergy of some foods and never got serious diseases. There was story of biting and picking of lips, when she was getting stress. She was reffered by oral medicine specialist to get the complete blood count and Ig E test, but all of the results were normal. The final diagnosis was Exfoliative cheilitis, and the differential diagnosis was Allergic Stomatitis. She was prescibed triamcinolone acetonide 0,1% topically on her lips for three times until seven days. The instruction to patient were drinking much water and avoiding her habit of biting and picking of lips. Conclusion : Severe tress will trigger more proinflamation cytokines to increase epidermal growth factor (EGF) of oral mucous, so that caused unremitting desquamation.
COMPARISON OF INHIBITORY ACTIVITY OF KELAKAI LEAVES EXTRACT WITH Ciprofloxacin AGAINST Aggregatibacter actinomycetemcomitans ATCC® 6514™ Dyah Setyorini; I Wayan Arya K. Firdaus; Beta Widya Oktiani
Dentino : Jurnal Kedokteran Gigi Vol 4, No 2 (2019)
Publisher : FKG Unlam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/dentino.v4i2.7054

Abstract

Background: Aggressive periodontitis is an inflammatory disease that exhibit rapid destruction of periodontal tissue and alveolar bone. The disease is caused by several factors, one of them is Aggregatibacter actinomycetemcomitans. The treatment to reduce the growth of Aggregatibacter actinomycetemcomitans is by giving spesific antibiotic like ciprofloxacin. Frequent and inappropriate use of antibiotics can cause side effects, so it needs alternative medicine that have antibacterial activity like kelakai leaves. Ethanol extract of kelakai leaves contains antibacterial compounds, such are flavonoid, tannin, steroid and alkaloid. Objective: The aim of this study was to compare the inhibitory activity of kelakai leaves extract (Stenochlaena palustris) with ciprofloxacin against Aggregatibacter actinomycetemcomitans. Method: This study was true experimental design with  post-test only design. The inhibitory activity test was performed using diffusion method in 5 treatment groups and 4 repetitions. The treatment groups were kelakai leaf extract with 25%, 50%, 75%, 100% concentrations  and 5µg ciprofloxacin. Inhibitory activity was measured by calculating the diameter of the clear zone (mm) that was formed on MHA. Results: The highest inhibition zone of kelakai leaf extract which found at concentration of 100% was 14.45 mm and the inhibition zone of ciprofloxacin was 26.28 mm. One way Anova test result (P = 0,000) and post hoc LSD test proved that there were significant differences of inhibition zones in each treatment group. Conclusion: Ethanol extract of kelakai leaves with concentration of 25%, 50%, 75% and 100% have inhibitory activity against Aggregatibacter actinomycetemcomitans but it is not as strong as ciprofloxacin.
TOXICITY TEST OF THE MIXED MOUTHWASH OF MAULI BANANA STEM AND BASIL LEAF AGAINST FIBROBLAST CELL STUDY IN VITRO Martha Vania Saputri; Amy Nindia Carabelly; I Wayan Arya K. Firdaus
Dentino : Jurnal Kedokteran Gigi Vol 4, No 2 (2019)
Publisher : FKG Unlam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/dentino.v4i2.7043

Abstract

Background: Mauli banana stem and basil leaf are herbal plants which can be used as an herbal mouthwash. People in South Kalimantan often use mauli banana stem to accelerate wound healing because of its antibacterial activity. Basil leaf are proven to inhibit bacterial growth. Mauli banana stem has a bitter taste, so to minimize that bitter taste, basil leaf can be used as a mixture. Basil leaf give fresh and aromatic effect. Extract of mauli banana stem is not toxic against fibroblast cell BHK-21 with 25% concentrate, but there is no research about the toxicity of a mixed mouthwash of mauli banana stem and basil leaf against fibroblast cell BHK-21 yet. Purpose: To analyze IC50 after treatment with a mixed mouthwash of mauli banana stem and basil leaf into the fibroblast cell BHK-21. Method: True experimental using the posttest only with control group design which consisted of 10 treatment groups with several concentrations. Those were 31,25 μg/mL, 62,5 μg/mL, 125 μg/mL, 250 μg/mL, 500 μg/mL, 1000 μg/mL, 2000 μg/mL and 4000 μg/mL. The toxicity test used MTT Assay as a method. This research was formed by 2 stages, which were preparation of fibroblast cell BHK-21 and the toxicity test. The cell viability counted with Freshney formula. Result: The result showed that the IC50 was 2183,43 μg/mL. Conclusion: The mixed mouthwash of mauli banana stem and basil leaf is not toxic against fibroblast cell BHK-21 because IC50 is 2183,43 μg/mL.
PENGARUH PERENDAMAN EKSTRAK DAUN KEMANGI 12,5% DAN BATANG PISANG MAULI 25% TERHADAP KEKERASAN PERMUKAAN RESIN AKRILIK Raudatul Izzah; I Wayan Arya K. Firdaus; Bayu Indra Sukmana
Dentin Vol 3, No 3 (2019)
Publisher : Universitas Lambung Mangkurat

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Abstract

ABSTRACTBackground: The most used denture base is heat cured acrylic resin. The use of dentures can cause denture stomatitis. The action taken to prevent denture stomatitis is by cleaning the denture. Chemical denture cleaners can reduce surface hardness of acrylic resin surfaces. Alternative natural denture cleanser is used to minimize the effects of chemical denture cleanser. The natural denture cleanser is using 12,5% Basil leaves and 25% Mauli banana stem extracts. Objective: The aim of this study was to analyze the comparison in surface hardness of heat cured acrylic resin which immersed in 12.5% Basil leaves and 25% Mauli banana stem extracts with alkaline peroxide and distilled water. Methods: This study was a true experimental study with pre and posttest with control group design, using 24 cylindrical type heat cured acrylic resin samples with a diameter of 30 mm and 5 mm thickness. The study used 3 treatment groups: 12.5% Basil leaves and 25% Mauli banana stem extracts, alkaline peroxide, and distilled water. Hardness testing was done using Vickers Hardness Testing. Results: The mean of the decrease in surface hardness value of heat cured acrylic resin which was immersed in extract solution, alkaline peroxide, distilled water are 1.40, 1.38, and 0.60 respectively. Data were analyzed statistically using One-Way ANOVA parametric test and Bonferroni Post Hoc test. Conclusion: The decrease in surface hardness value of heat cured type acrylic resin immersed in 12.5% Basil leaves and 25% Mauli banana stem extracts is higher than those immersed in alkaline peroxide and distilled water.Keywords: Basil leaves and Mauli banana stem extracts, surface hardness, heat cured acrylic resin ABSTRAKLatar belakang: Basis gigi tiruan yang banyak digunakan adalah resin akrilik tipe heat cured. Penggunaan gigi tiruan dapat menyebabkan denture stomatitis. Tindakan yang dilakukan untuk mencegah denture stomatitis yaitu dengan melakukan pembersihan gigi tiruan. Pembersih gigi tiruan berbahan kimia dapat menurunkan kekerasan permukaan resin akrilik. Alternatif pembersih gigi tiruan alami digunakan untuk meminimalisir efek yang ditimbulkan oleh pembersih gigi tiruan berbahan kimia. Bahan pembersih gigi tiruan alami yang dipakai yaitu ekstrak daun kemangi 12,5% dan batang pisang mauli 25%. Tujuan: penelitian ini bertujuan untuk menganalisis perbandingan kekerasan basis resin akrilik tipe heat cured yang direndam pada ekstrak daun kemangi 12,5% dan batang pisang mauli 25% dengan alkalin peroksida dan akuades. Metode: penelitian ini merupakan penelitian true experimental dengan pre and posttest with control group design, menggunakan 24 sampel resin akrilik tipe heat cured berbentuk silindris dengan diameter 30 mm dan tebal 5 mm. Penelitian menggunakan 3 kelompok perlakuan: ekstrak daun kemangi 12,5% dan batang pisang mauli 25%, alkalin peroksida dan akuades. Uji kekerasan dilakukan menggunakan Vickers Hardness Testing. Hasil penelitian: Rerata nilai penurunan kekerasan permukaan resin akrilik tipe heat cured yang direndam pada larutan ekstrak daun kemangi 12,5% dan batang pisang mauli 25% sebesar 1,40, alkalin peroksida sebesar 1,38 dan akuades sebesar 0,60. Data dianalisis secara statistik menggunakan uji parametrik One Way ANOVA dan uji Post Hoc Bonferroni. Kesimpulan: Nilai penurunan kekerasan permukaan resin akrilik tipe heat cured yang direndam pada ekstrak daun kemangi 12,5% dan batang pisang mauli 25% lebih tinggi dibandingkan dengan resin akrilik tipe heat cured yang direndam pada alkalin peroksida dan akuades.Kata-kata kunci: Ekstrak daun kemangi dan batang pisang mauli, kekerasan permukaan, resin akrilik tipe heat cured
PENGARUH PERENDAMAN EKSTRAK BATANG PISANG MAULI 25% DAN DAUN KEMANGI 12,5% TERHADAP NILAI KEKASARAN PERMUKAAN (Nilai kekasaran permukaan basis akrilik menggunakan resin akrilik tipe heat cured) Ghina Ulya Rifdayanti; I Wayan Arya K. Firdaus; Bayu Indra Sukmana
Dentin Vol 3, No 3 (2019)
Publisher : Universitas Lambung Mangkurat

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Abstract

ABSTRACTBackground: Denture cleanser that are often used are in the form of chemical solvent. Chemicals have side effects which may increase the degree of surface roughness in acrylic resin-based dentures. Surface roughness causes plaque formation and accumulation of Candida albicans which is the cause of denture stomatitis. Alternative can be used as denture cleanser such as 25% mauli banana stem extract and 12.5% basil leave. The combination of these two extracts is expected to have better effectiveness against Candida albicans by not changing the nature of the acrylic resin base is surface roughness. Objective: The aim of this study was to determine the effect of value surface roughness heat cured acrylic resin immersed with extract of 25% mauli banana stem and 12.5% basil leave. Methods: This research was a true experimental laboratory pretest and postest with control group design, using a simple random sampling technique. Sample of acrylic resin was rectangular 65 x 10 x 3.3 mm. The samples were 24 in number and divided into 3 treatment groups, 25% mauli banana stem and 12.5% basil leave extract, alkaline peroxide, and aquadest. Surface roughness measurement was performed using surface roughness tester. Result: The mean results of surface roughness increase in 25% banana mauli stem and 12.5% basil leave extract, alkaline peroxide, and aquadest group were 0.0613 µm, 0.0663 µm, and 0.0425 µm respectively. Data were analyzed using One Way ANOVA and Bonferroni Post Hoc test. Conclusion: An increase in surface roughness of acrylic resin on alkaline peroxide immersion is higher than 25% mauli banana stem and 12.5% basil leave extract, and aquadest.Keywords: Heat cured acrylic resin, mauli banana stem and basil leaves extract, surface roughness  ABSTRAKLatar belakang: Pembersih gigi tiruan yang sering digunakan adalah perendaman secara kimiawi. Bahan kimia memiliki efek samping dapat menyebabkan kekasaran permukaan pada basis gigi tiruan resin akrilik. Kekasaran permukaan menyebabkan penumpukan plak dan akumulasi Candida albicans yang merupakan penyebab denture stomatitis. Bahan alternatif yang dapat digunakan sebagai pembersih gigi tiruan yaitu ekstrak batang pisang mauli 25% dan daun kemangi 12,5%. Kombinasi antara kedua eksrtrak tersebut diharapkan memiliki efektifitas yang lebih baik terhadap Candida albicans dengan tidak merubah sifat basis resin akrilik yaitu kekasaran permukaan. Tujuan penelitian: Penelitian ini bertujuan untuk mengetahui pengaruh nilai kekasaran permukaan resin akrilik tipe heat cured yang direndam dengan ekstrak batang pisang mauli 25% dan daun kemangi 12,5%. Metode: Penelitian eksperimental laboratorium murni dengan Pretest and Postest with Control Group Design, pengambilan sampel menggunakan teknik simple random sampling, bentuk sampel resin akrilik tipe heat cured persegi panjang 65 x 10 x 3,3 mm berjumlah 24 sampel yang dibagi menjadi 3 kelompok perlakuan yaitu ekstrak batang pisang mauli 25% dan daun kemangi 12,5%, alkalin peroksida, dan akuades. Pengukuran kekasaran permukaan menggunakan alat surface roughness tester. Hasil penelitian: Hasil rerata peningkatan kekasaran permukaan yang dihasilkan pada ekstrak batang pisang mauli 25% dan daun kemangi 12,5%, alkalin peroksida, dan akuades sebesar (0,0613 µm, 0,0663 µm dan 0,0425 µm). Data dianalisis menggunakan uji statistik One Way ANOVA dan dilanjutkan dengan uji Pos Hoc Bonferroni. Kesimpulan: Peningkatan kekasaran permukaan resin akrilik pada perendaman alkalin peroksida lebih tinggi dibandingkan dengan ekstrak batang pisang mauli 25% dan daun kemangi 12,5%, dan akuades.Kata-kata kunci: Ekstrak batang pisang mauli dan daun kemangi, kekasaran permukaan, resin akrilik tipe heat cured.
Co-Authors Amy Nindia Carabelly Bayu Indra Sukmana Beta Widya Oktiani Dyah Setyorini Fitriana Caessar Pertiwi Ghina Ulya Rifdayanti Maharani Laillyza Apriasari Martha Vania Saputri Raudatul Izzah