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All Journal Dentino: Jurnal Kedokteran Gigi Dentin Prosiding Konferensi Nasional PKM-CSR
Beta Widya Oktiani
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Humanities Control & Systems Engineering Dentistry Economics, Econometrics & Finance Education Environmental Science Nursing Public Health Social Sciences

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ANTIBACTERIAL EFFECTIVITY TEST OF ULIN BARK EXTRACT (Eusideroxylon zwageri) ON THE GROWTH OF Porphyromonas gingivalis Della Isnadya Noor; I Wayan Arya Krishnawan Firdaus; Beta Widya Oktiani
Dentino : Jurnal Kedokteran Gigi Vol 6, No 1 (2021)
Publisher : FKG Unlam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/dentino.v6i1.10638

Abstract

ABSTRACTBackground: Chronic periodontitis is an infectious disease caused by bacterial colonization of dental plaque. Bacteria that play a role in chronic periodontitis is Porphyromonas gingivalis. One of mouthwash that reduce the number of P.gingivalis colonies is chlorhexidine 0.2%. Long term use of chlorhexidine 0.2% can cause some side effects to the oral cavity, so we need an alternative mouthwash from natural ingredients that can reduce the side effects of chlorhexidine 0.2%. Ulin bark extract contains phenolic, flavonoid, tannin, alkaloid, terpenoid, and saponin which can be an alternative base for mouthwash besides chlorhexidine 0.2%. Purpose: It is to analyze antibacterial effectivity of ulin bark extract on the growth of Porphyromonas gingivalis. Methods: This study was using true experimental research and post-test only with control group design, that used 9 treatment groups with 4 replications, that were 5%, 10%, 20%, 40%, 60%, 80%, 100% concentrations, chlorhexidine 0.2%, and sterile aquadest against P. gingivalis. The total samples were as many as 36. Result: One Way ANOVA and Post-Hoc Games Howell tests showed that the average absorbance values has a significant difference, then Kruskall Wallis and Post Hoc Mann Whitney tests showed the number of colonies with significant differences. MIC in this study was at 5% concentration and MBC was at 20% concentration.. Conclusion: Ulin bark extract with 40% concentration has an absorbance value equivalent to 0.2% chlorhexidine and 20% concentration has a bactericidal effects equivalent to 0.2% chlorhexidine against the growth of P.gingivalis.Keywords:Antibacterial, Dilution method,Porphyromonas gingivalis, Ulin Bark Extract.
PERBEDAAN SKOR INDEKS PLAK SEBELUM DAN SESUDAH DILAKUKAN PENYULUHAN DENGAN MEDIA VIDEO DAN MODEL STUDI Tinjauan Pada Siswa Tunarungu di SMPLB dan SMALB B Dharma Wanita Banjarmasin Riznika Riznika; Rosihan Adhani; Beta Widya Oktiani; Isnur Hatta
Dentino : Jurnal Kedokteran Gigi Vol 2, No 1 (2017)
Publisher : FKG Unlam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/dentino.v2i1.2599

Abstract

ABSTRACTBackground: The severity of oral health problem of hearing-impaired people is worse than normal-hearing population (tooth caries prevalence 83,92%). Hearing-impaired people have more untreated dental problems. One of the dental problems is plaque. Plaque can be prevented by brushing teeth correctly, which can be taught through socialization. Purpose: This research was aimed to find out the different plaque index scores before and after the socialization through using video and study model to hearing-impaired students at SMPLB and SMALB B Dharma Wanita Banjarmasin. Methods: This research was the quasi experimental, which used pre and posttest with control group design. The research subjects were 24 hearing-impaired students: 12 SMPLB students and 12 SMALB B students. All the subjects had plaques index scores pre-examination and pre-scoring through O’Leary method. Experimental group was socialized through video and study model, and was instructed to brush teeth two times a day for seven days: after having breakfast and before sleeping, whereas control group was not socialized and instructed. After the seventh day, all the subjects had their plaque index score re-examined and re-scored. Results: Showed decrease on experimental group’s mean score (the first mean 41,35±18,76, the final mean 20,37±8,36), the paired t-test result was significant(p)=0,001 which showed significant difference. The final mean score of experimental group (20,37±8,36) was different from the control group’s mean score (60,91±24,66), the independent t-test was p=0,000, which showed significant difference. Conclusion: There was different plaque index scores before and after the socialization, and also between the experimental and control group.Keywords: plaque, hearing-impaired person, socialization, video, study model
THE EFFECTS OF PANORAMIC RADIOGRAPHY ON GINGIVAL CREVICULAR FLUID VOLUME IN GINGIVITIS Rini Rahmiyati; Didit Aspriyanto; Beta Widya Oktiani
Dentino : Jurnal Kedokteran Gigi Vol 6, No 2 (2021)
Publisher : FKG Unlam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/dentino.v6i2.11995

Abstract

Background: Gingival crevicular fluid (GCF) is a biological fluid derived from the gingival sulcus and can be elevated in the inflammatory state of periodontal tissue, such as gingivitis. In previous studies, the number of GCF could also increase after panoramic radiographic exposure. Increase in GCF due to panoramic radiography is a sign of cell damage. Objective: To analyze the effects of panoramic radiography on the volume of GCF in wistar rats with gingivitis. Method: This type of research was true experimental with post test only and control group design. The sampling technique used was simple random sampling. A total of 25 wistar rats were classified into two control groups without exposure (state without gingivitis and gingivitis) and three groups treatment of exposure (state of gingivitis with 1 time, 2 times, and 3 times the exposure). GCF sampling using filter paper was carried out 10 minutes after panoramic radiographic exposure. The filter paper was stained by 2% ninhydrin solution, after that the GCF volume was calculated. Results: There was a significant difference in the number of GCF (p<0.05) in the group without gingivitis and exposure compared to all other groups, the gingivitis group without exposure compared to the gingivitis group with 3 times exposure, and the gingivitis group with 1 time exposure compared to the gingivitis group 3 times with exposure. Conclusion: Panoramic radiography can cause an increase in the volume of GCF in wistar rats with gingivitis. Keywords: GCF, gingivitis, panoramic radiography
ANTIBACTERIAL EFFECTIVENESS TEST OF RAMANIA LEAVES (Bouea macrophylla Griffith) FLAVONOIDS EXTRACT ON Aggregatibacter actinomycetemcomitans BACTERIA CAUSING AGGRESSIVE PERIODONTITIS Noor Khalishah; Beta Widya Oktiani; Rosihan Adhani
Dentino : Jurnal Kedokteran Gigi Vol 6, No 1 (2021)
Publisher : FKG Unlam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/dentino.v6i1.10636

Abstract

ABSTRACTBackground: Aggressive periodontitis is a disease that is mostly suffered by young patients with minimal local factors. The prevalence of this disease is approximately 8% in Indonesia, where Aggregatibacter actinomycetemcomitans bacteria are presented as dominant bacteria in this disease. Aggregatibacter actinomycetemcomitans is a gram-negative bacterium that is sensitive to flavonoids. Ramania leaves are natural substances which contain flavonoids. Flavonoids in ramania leaves can be applied as an antibacterial substance. Objectives: The objectives of this research are to determine and analyze the minimum inhibitory concentration and the minimum bactericidal concentration of flavonoids extract from ramania leaves at the concentration of 0.1%, 0.3%, and 0.5%, and analyze the antibacterial effectiveness on Aggregatibacter actinomycetemcomitans. Methods: True experimental design with pre and post-test with control group design was applied in this study. Antibacterial test using broth and agar dilution method was performed in a total of 5 treatment groups with 6 repetitions. Results:  All exract at 0.1%, 0.3% and 0.5% concentration demonstrated the ability to inhibit bacterial growth, and the minimum bactericidal concentration was found at 0.5% concentration. Result test for One way ANNOVA on minimum inhibitory concentration and Kruskal wallis test on minimum bactericidal concentration exhibited significant value of less than 0.05 presenting significant differences in some groups. Conclusion:  Minimum inhibitory concentration of ramania leaves flavonoid extract is effective on Aggregatibacter actinomycetemcomitans at the concentration of 0.1% and minimum bactericidal concentration of ramania leaves flavonoid extract is more effective at the concentration of 0.5%.Keywords: Aggregatibacter Actinomycetemcomitans, Aggressive Periodontitis, Dilution Method, Flavonoids, Ramania Leaves.
THE EFFECT OF PANORAMIC RADIOGRAPHY ON THE NUMBER OF MICRONUCLUES IN PERIODONTITIS Khairunnisa Puspita Sari; Didit Aspriyanto; Beta Widya Oktiani
Dentino : Jurnal Kedokteran Gigi Vol 6, No 2 (2021)
Publisher : FKG Unlam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/dentino.v6i2.11996

Abstract

Background: Periodontitis is a periodontal disease that can cause an increase in Reactive Oxygen Species (ROS). The increase in ROS concentration will cause oxidative stress which has a role in DNA damage. In the case of periodontitis, panoramic radiography plays an important role in showing generalized horizontal bone loss. Radiation on panoramic radiography may cause DNA damage. DNA damage that occurs due to periodontitis or panoramic radiographic radiation exposure is characterized by the formation of micronuclei in gingival epithelial cells. Objective: To determine the effect of panoramic radiography on the number of micronuclei in Wistar rats with periodontitis. Method: This study was true experimental with post-test only and control group design. This study used 15 male Wistar rats which divided into 5 groups. Result: The average number of micronuclei in the normal group and 1 time exposures was 4 compared to the periodontitis group without exposure was 1.67, periodontitis and 1 time exposures was 8, periodontitis and 2 times the exposure was 15.67, and in the periodontitis and 3 times the exposure was 42.67. Result of One-Way Annova test and Post Hoc Bonferroni test indicated that signigicant changes in the number of micronuclei was seen between the normal group with 1 time exposure to the periodontitis group with 2 times and 3 times exposure and int the periodontitis group without exposure to the periodontitis group with 2 times and 3 times exposure. Conclusion: Panoramic radiograph X ray radiation and periodontitis can cause changes in the number of micronuclei in wistar rats. Keywords: , Micronucleus, Panoramic Radiography, Periodontitis.
COMPARISON OF INHIBITORY ACTIVITY OF KELAKAI LEAVES EXTRACT WITH Ciprofloxacin AGAINST Aggregatibacter actinomycetemcomitans ATCC® 6514™ Dyah Setyorini; I Wayan Arya K. Firdaus; Beta Widya Oktiani
Dentino : Jurnal Kedokteran Gigi Vol 4, No 2 (2019)
Publisher : FKG Unlam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/dentino.v4i2.7054

Abstract

Background: Aggressive periodontitis is an inflammatory disease that exhibit rapid destruction of periodontal tissue and alveolar bone. The disease is caused by several factors, one of them is Aggregatibacter actinomycetemcomitans. The treatment to reduce the growth of Aggregatibacter actinomycetemcomitans is by giving spesific antibiotic like ciprofloxacin. Frequent and inappropriate use of antibiotics can cause side effects, so it needs alternative medicine that have antibacterial activity like kelakai leaves. Ethanol extract of kelakai leaves contains antibacterial compounds, such are flavonoid, tannin, steroid and alkaloid. Objective: The aim of this study was to compare the inhibitory activity of kelakai leaves extract (Stenochlaena palustris) with ciprofloxacin against Aggregatibacter actinomycetemcomitans. Method: This study was true experimental design with  post-test only design. The inhibitory activity test was performed using diffusion method in 5 treatment groups and 4 repetitions. The treatment groups were kelakai leaf extract with 25%, 50%, 75%, 100% concentrations  and 5µg ciprofloxacin. Inhibitory activity was measured by calculating the diameter of the clear zone (mm) that was formed on MHA. Results: The highest inhibition zone of kelakai leaf extract which found at concentration of 100% was 14.45 mm and the inhibition zone of ciprofloxacin was 26.28 mm. One way Anova test result (P = 0,000) and post hoc LSD test proved that there were significant differences of inhibition zones in each treatment group. Conclusion: Ethanol extract of kelakai leaves with concentration of 25%, 50%, 75% and 100% have inhibitory activity against Aggregatibacter actinomycetemcomitans but it is not as strong as ciprofloxacin.
ANTIOXIDANT ACTIVITY POTENCY OF CHITOSAN FROM HARUAN (CHANNA STRIATA) SCALES Deby Kania Tri Putri; Beta Widya Oktiani; Candra Candra; Rosihan Adhani
Dentino : Jurnal Kedokteran Gigi Vol 5, No 2 (2020)
Publisher : FKG Unlam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/dentino.v5i2.8951

Abstract

ABSTRACTBackground: Stimulation of inflammatory cells such as polymorphonuclear cells (PMN) will initiate to release free radicals in destroying bacteria. Excessive free radicals can damage cells in the body. Antioxidants as one of the body's defense systems will neutralize existing free radicals. Chitosan from Haruan scales in addition to being antimicrobial turned out to also be antioxidants. Objective: To know the antioxidant potential of chitosan from haruan (Channa striata) scales. Methods: This study is a pure experimental (true experimental design) with a post-test only with control group design using two treatment groups, with quantitative tests to calculate the amount of antioxidant activity of chitosan from fish scales using DPPH radical reduction method. The first treatment is 4, 6, 8 and 10 ppm standard ascorbic acid concentration with DPPH solution as positive control, and the second treatment is 200, 250, 300 and 350 ppm chitosan from Haruan scales concentration with DPPH solution. Results: The results showed that chitosan from Haruan fish scales have antioxidant activity with 50.513% percentage of inhibition at a maximum concentration of 350 ppm with an IC50 value of 356.98 ppm. The results of the independent T-test showed that there was a significant difference between the inhibition percentage of chitosan from haruan fish scales and the inhibition percentage of ascorbic acid (p = 0,000) (p <0.05). Conclusion: Chitosan from Haruan fish scales proved to have antioxidant activity.Keywords: Antioxidants, Chitosan, Haruan fish scalesABSTRACTBackground: Stimulation of inflammatory cells such as polymorphonuclear cells (PMN) will initiate to release free radicals in destroying bacteria. Excessive free radicals can damage cells in the body. Antioxidants as one of the body's defense systems will neutralize existing free radicals. Chitosan from Haruan scales in addition to being antimicrobial turned out to also be antioxidants. Objective: To know the antioxidant potential of chitosan from haruan (Channa striata) scales. Methods: This study is a pure experimental (true experimental design) with a post-test only with control group design using two treatment groups, with quantitative tests to calculate the amount of antioxidant activity of chitosan from fish scales using DPPH radical reduction method. The first treatment is 4, 6, 8 and 10 ppm standard ascorbic acid concentration with DPPH solution as positive control, and the second treatment is 200, 250, 300 and 350 ppm chitosan from Haruan scales concentration with DPPH solution. Results: The results showed that chitosan from Haruan fish scales have antioxidant activity with 50.513% percentage of inhibition at a maximum concentration of 350 ppm with an IC50 value of 356.98 ppm. The results of the independent T-test showed that there was a significant difference between the inhibition percentage of chitosan from haruan fish scales and the inhibition percentage of ascorbic acid (p = 0,000) (p <0.05). Conclusion: Chitosan from Haruan fish scales proved to have antioxidant activity.    Keywords: Antioxidants, Chitosan, Haruan fish scales.
THE EFFECT OF FLAVONOID PROPOLIS KELULUT (Trigona spp) EXTRACT ON MACROPHAGE CELL NUMBER IN PERIODONTITIS (IN VIVO STUDY IN MALE WISTAR RATE (Rattus novergicus) GINGIVA) Makfiyah Saidah; Beta Widya Oktiani; Irham Taufiqurrahman
Dentino : Jurnal Kedokteran Gigi Vol 5, No 1 (2020)
Publisher : FKG Unlam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/dentino.v5i1.8117

Abstract

Background : Periodontitis is a condition where there is an increase in the number of inflammatory cells, namely macrophages in periodontal tissue. Macrophag cell is 12-15μm in oval shape cell with purplish blue cytoplasm and this cell’s function is to phagocytes bacteria and infiltrate gingival tissue. Propolis kelulut contains flavonoid that have an anti-inflammatory effect by suppressing the signal pathway p38 MAPK, JNK 1/2 and NF-kB that it can reduce the number of macrophage cells in inflammatory periodontal tissues. Objective: The purpose of this study was to determine the effect of 0.5 mg dose flavonoid propolis extract on the number of macrophage cells in gingiva wistar rats that have been made into a periodontitis condition. Method: This study used a pure experimental method with a post test only with control group design. There were 9 treatment groups, including flavonoid propolis extract on 1,3,5 days, ibuprofen gel on 1,3,5 days and negative control on 1,3 dan 5 days. Results: There was an effect of giving 0.5 mg flavonoids propolis kelulut extract to the number of macrophage cells in periodontitis. Conclusion: Flavonoid propolis kelulut extract has an effect in increasing the number of macrophage cells on day 3 and decreasing the number of macrophage cells on the 5th day.
PENGARUH EKSTRAK FLAVONOID PROPOLIS KELULUT (G.thoracica) TERHADAP JUMLAH SEL NETROFIL PADA PERIODONTITIS (Studi In Vivo Pada Tikus Wistar (Rattus norvegicus) Jantan) Adela Tamara; Beta Widya Oktiani; Irham Taufiqurrahman
Dentin Vol 3, No 1 (2019)
Publisher : Universitas Lambung Mangkurat

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Latar Belakang:Periodontitis adalah inflamasidestruktifpadajaringan periodontal yang disebabkanoleh bakteri anaerob seperti Actinobacillus actinomycetemcomitans (Aa), Porphyromonas gingivalis (Pg), dan Prevotellaintermedia yang ditandai dengan terbentuknyapoket, kegoyangan gigi, hilangnya perlekatan dan resesi gingiva. Periodontitis dapat ditangani dengan pemberian terapi scaling dan root planning serta ditunjang pemberian obat-obatan anti-inflamasi  seperti ibuprofen. Pemberian ibuprofen dalam jangka panjang dapat menimbulkan efek samping sehingga memerlukan alternatif lain seperti penggunaan bahan alam. Bahan alam yang dapat dijadikan alternatif adalah propolis kelulut G.thoracica. Propolis kelulut G.thoracica memiliki kandungan flavonoid yang berperan sebagai zat anti-inflamasi sehingga akan menurunkan jumlah sel netrofil.  Tujuan: Untuk membuktikan pengaruh ekstrak flavonoid propolis kelulut (G.thoracica) terhadap jumlah sel netrofil pada tikus wistar (Rattus norvegicus) jantan yang diinduksi periodontitis.Metode:Penelitian ini menggunakan 36 ekor tikus yang dibagi menjadi 9 kelompok: kelompok kontrol negatif yang tidak diberi perlakuan sama sekali,  kelompok perlakuan yang diberikan ekstrak flavonoid propolis kelulut dengan konsentrasi 0,5 mg, dan kelompok kontrol positif ibuprofen.Hasil:Hasil uji statistik One-way ANOVA menunjukkan terdapat perbedaan bermakna pada setiap kelompok perlakuan di hari ke-1 (p=0.000), hari ke-3 (p=0.001), dan hari ke-5 (p=0.002). Uji Post-Hoc Bonferroni untuk rerata jumlah sel netrofil di hari 1, 3, dan 5 menunjukkan terdapat perbedaan bermakna (p<0.05) pada tiap kelompok. Kesimpulan:Terdapat pengaruh ekstrak flavonoid propolis kelulut terhadap peningkatan jumlah sel netrofil di hari ke-1 dan penurunan jumlah sel netrofil di hari ke-3 dan ke-5 pada tikus wistar yang diinduksi periodontitis.Kata Kunci:  Ekstrak flavonoid propolis kelulut, Jumlah sel netrofil, Periodontitis.   (Studi In VivoPada Tikus Wistar (Rattus norvegicus) Jantan)
PREVALENSI DAN ANALISIS FAKTOR RISIKO KEJADIAN GINGIVITIS DAN PERIODONTITIS PADA PASIEN DIABETES MELITUS (Literature Review) Miftakhu Rizkiyah; Beta Widya Oktiani; Ika Kusuma Wardani
Dentin Vol 5, No 1 (2021)
Publisher : Universitas Lambung Mangkurat

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Abstract

ABSTRACTBackground: Diabetes mellitus is associated with periodontal disease and considered as a risk factor for periodontal disease. Periodontal disease can be divided into gingivitis and periodontitis. Age, gender, blood glucose level, and duration of diabetes mellitus were considered as risk factors of gingivitis and periodontitis. Objective: To determine the prevalence and relationship between age, sex, blood glucose level, and duration of diabetes mellitus with gingivitis and periodontitis. Method: This study used a literature review research type. Data search was performed using PubMed, Google Scholar, and Science Direct. Literature screening based on the inclusions and continued with article selection. The selected literature is then extracted, analyzed, and synthesized. Results: A total of 38 articles selected because they match inclusion and exclusion criteria which using the keywords diabetes mellitus, risk factors, gingivitis, periodontal disease, periodontitis, and prevalence; published in 2011-2020; written in Indonesian or English; available in full text; and not duplication. Conclusion: Patients with diabetes mellitus experience more periodontitis. Age, sex, and duration of diabetes mellitus were associated with gingivitis and periodontitis. Keywords : Diabetes mellitus, Gingivitis, Periodontal Disease, Periodontitis, Prevalence, dan Risk factors.ABSTRAKLatar belakang: Diabetes melitus berhubungan dengan penyakit periodontal dan dianggap sebagai faktor risiko penyakit periodontal. Penyakit periodontal secara umum dapat dibedakan menjadi gingivitis dan periodontitis. Usia, jenis kelamin, kadar glukosa darah, dan durasi menderita diabetes melitus dianggap sebagai faktor risiko kejadian gingivitis dan periodontitis. Tujuan: Mengetahui prevalensi dan hubungan usia, jenis kelamin, kadar glukosa darah, dan durasi menderita diabetes melitus dengan kejadian gingivitis dan periodontitis. Metode: Penelitian ini menggunakan jenis penelitian literature review. Pencarian sumber data dilakukan menggunakan PubMed, Google Scholar,  dan Science Direct. Screening literature berdasarkan inklusi dan dilanjutkan dengan seleksi artikel. Literature yang telah dipilih kemudian diekstraksi, dianalisis, dan disintesis. Hasil: Terdapat 38 artikel yang dipilih sesuai kriteria inklusi dan eksklusi yaitu menggunakan kata kunci diabetes melitus, faktor risiko, gingivitis, penyakit periodontal, periodontitis, dan prevalensi; dipublikasi tahun 2011-2020; ditulis dalam Bahasa Indonesia atau Inggris; tersedia dalam bentuk full text; dan bukan duplikasi. Kesimpulan: Pasien diabetes melitus lebih banyak mengalami periodontitis. Faktor risiko seperti usia, jenis kelamin, dan durasi menderita diabetes melitus berhubungan dengan kejadian gingivitis dan periodontitis. Kata kunci : Diabetes melitus, Faktor risiko, Gingivitis, Penyakit periodontal, Periodontitis, dan Prevalensi.
Co-Authors Adela Tamara Anisa Novia Farrasti Anugrah Qotrunnada Hakim Aspriyanto, Didit Bimo Gondo Arum Brachmedio Barito Syech Erlangga Candra Candra Debby Saputera, Debby Della Isnadya Noor Dewi Nurdiana Duhan Kanzu Balad Dyah Setyorini Elsa Ayu Amanda Fransiska U.A Panjaitan Hatta, Isnur Hilyatul Auliya I Wayan Arya K. Firdaus I Wayan Arya Krishnawan Firdaus Ika Kusuma Wardani Juli Harnida Purwaningayu Khairunnisa Puspita Sari Laili Nurul Islami Makfiyah Saidah Melati Ainun Nisa Miftakhu Rizkiyah Nabila Nabila Noor Khalishah Nur Alam Prilly Sonya Puteri Rahmad Arifin Renie Kumala Dewi Rini Rahmiyati Riznika Riznika Rosihan Adhani, Rosihan Sarifah, Norlaila Sherli Diana Siti Khairiah Tri Putri, Deby Kania Utami, Juliyatin Putri Veren Yosi Erinda Yusrinie Wasiaturrahmah