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All Journal Jurnal Sain Veteriner Bioma : Berkala Ilmiah Biologi JURNAL ANATOMI FISIOLOGI SAINS DAN MATEMATIKA AL KAUNIYAH BIOMA Biosaintifika: Journal of Biology & Biology Education Buletin Anatomi dan Fisiologi (Bulletin of Anatomy and Physiology)
Siti Muflichatun Mardiati
Jurusan Biologi, Fakultas Sains Dan Matematika, Universitas Diponegoro Jl. Prof. Soedarto, SH, Tembalang, Semarang
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Health Professions Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Public Health Veterinary

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HEMATOLOGI KELINCI (Lepus sp.) SETELAH PERLAKUAN IMPLANTASI MATERIAL STAINLESS STEEL AISI 316L SELAMA 2,5 BULAN Indriati, Martha Dian; Tana, Silvana; Mardiati, Siti Muflichatun
BULETIN ANATOMI DAN FISIOLOGI dh SELLULA Vol 23, No 2 (2015): Volume XXIII, Nomor 2, Oktober 2015
Publisher : BULETIN ANATOMI DAN FISIOLOGI dh SELLULA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (377.026 KB) | DOI: 10.14710/baf.v23i2.10020

Abstract

Stainless Steel AISI 316L are one of the implant material used in the manufacture of artificial hip joint. Testing the effect of prior implantation needs to be done in animal models such as rabbits before applied in the human body. Future integration of the implant in the body of an animal model takes 4-6 weeks. This study aimed to analyze the body's physiological response to the implantation of an animal model of AISI 316L Stainless Steel for 2.5 months seen from the aspect of the number of erythrocytes, leukocytes and hemoglobin animal models as part of the basic data for further research in order to figure out orthopedic implants that can be used by the body. This study compares the P0: control rabbits and Ps: rabbits given the implants Stainless Steel AISI 316L. The parameters measured were the number of erythrocytes, leukocyte count and hemoglobin levels, feed intake and body weight were analyzed by Independent Sample T test with SPSS procedures at 95% significance level. The results showed red cell count, leukocyte count and hemoglobin levels did not differ significantly (p> 0.05) between the control treatment. It can be concluded that the use of stainless steel AISI 316L for 2.5 months had no effect on animal physiology and is a material that can be used by the body.   Keywords: Rabbit, stainless steel AISI 316L, hematologic
Perbandingan Struktur Histologi Magnum pada Itik Magelang, Itik Tegal dan Itik Pengging Yuriwati, Febry Nurmala; Mardiati, Siti Muflichatun; Tana, Silvana
BULETIN ANATOMI DAN FISIOLOGI dh SELLULA Vol 24, No 1 (2016): Volume 24, nomor 1, tahun 2016
Publisher : BULETIN ANATOMI DAN FISIOLOGI dh SELLULA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (539.603 KB) | DOI: 10.14710/baf.v24i1.11696

Abstract

Duck is one of comodity in livestock which its productivity needs to be improved. The duck productivity is related to physiological function of the reproductive ogans. Magelang ducks, Tegal ducks and Pengging ducks have the potential to be developed. This research was aimed for comparing magnum hystology structure among Magelang Duck, Tegal Duck, and Pengging Duck. There were six ducks on each breed and at the age of six months old taken from breeding and cultivation ofnon-ruminant livestock, Banyubiru, regency Ambarawa, Used parafin method by Hematoxylin and Eosin staining. The data of magnum histology structure of magelang duck, tegal duck, and pengging duck that descriptively analyzed with captured and microscopic observation at first. Data of long reproductive tract, reproductive tract weight and ovarian weight were analyzed by using Analysis of Varian (ANOVA), by a further test of  Least Significant Differences (LSD) test at the level of 95%. Data analized of long reproductive tract, reproductive tract weight and ovarian weight did not show the significant difference. The histological structure on Magelang duck, Tegal duck and Pengging duck showed epithelial cells differentiated into ciliated epithelial cells and goblet cells and sub epithelial gland that had developed. It was concluded the magnum histological structure its not difference on Magelang ducks, Tegal ducks and Pengging ducks on 6 months old.   Keywords: local duck, histology, magnum
Kondisi Ovarium dan Saluran Reproduksi Setelah Pemberian Cahaya Monokromatik pada Puyuh (Coturnix coturnix japonica) Desly, Desly; Saraswati, Tyas Rini; Mardiati, Siti Muflichatun
BULETIN ANATOMI DAN FISIOLOGI dh SELLULA Vol 24, No 1 (2016): Volume 24, nomor 1, tahun 2016
Publisher : BULETIN ANATOMI DAN FISIOLOGI dh SELLULA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (426.278 KB) | DOI: 10.14710/baf.v24i1.11687

Abstract

Quail Product (Coturnix coturnix japonica) in the form of meat and eggs is a variation of the source of animal protein for human consumption. Like other poultry cultivation of intensive, maintaining the scale cultivation of quail in need of maintenance and good governance to obtain maximum results and profitable. One of the important things in a maintenance program is a good quail cage lighting. Quail is an organism that is sensitive to stimuli of light, light is required to regulate various biological processes, including the reproductive system. Light energy can be obtained from natural and artificial light. This research aims to use artificial light, which is monochromatic light to obtain basic information about aspects of the reproductive system of quail regarding the condition of the ovaries and reproductive tract. Monochromatic light is a type of visible light with the frequency of a single wavelength. Treatment consists of lighting from above 4 groups, namely the use of monochromatic light LED lights red, green, and blue with 15 lux and the light intensity control using a 15 watt incandescent bulb. All treatments began at 4-week-old quail to 9 weeks, from 5:00 p.m. to 07:00 o'clock. The parameters in this study were ovarian weight, number of hierarchical follicles, length and weight of the reproductive tract. This study using Completely Randomized Design (CRD) with replications is not the same. Data generated in this study were analyzed using the Analysis Of Variance (ANOVA) if there is a real difference then conducted further tests duncan. The results of this study showed that monochromatic light of red, green, and blue can affect ovarian weight and the weight of the reproductive tract, but has not given a real influence on the length of the reproductive tract and the number of follicles. In conclusion, the artificial light in the form of monochromatic light can be used as an alternative to the cultivation of quail to produce an optimal product.
Potensi Bahan Antiaging dari Ekstrak Ikan Gabus (Channa striata) terhadap Perbaikan Histo-Morfologi Hipokampus Sunarno, Sunarno; Mardiati, Siti Muflichatun; Suprihatin, Teguh
BULETIN ANATOMI DAN FISIOLOGI dh SELLULA Vol 23, No 1 (2015): VOLUME XXIII, NOMOR 1, MARET 2015
Publisher : BULETIN ANATOMI DAN FISIOLOGI dh SELLULA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (281.956 KB) | DOI: 10.14710/baf.v23i1.8738

Abstract

Penuaan kronologis dan penuaan yang dipicu stres oksidatif menjadi faktor utama penyebab percepatan penuaan hipokampus. Penuaan bagian wilayah otak ini ditandai dengan penurunan fungsi neuron yang mempunyai keterkaitan dengan deplesi glutation, perubahan morfologi dan struktur jaringan. Penuaan hipokampus juga mempunyai keterkaitan dengan penurunan kemampuan belajar-mengingat dan perilaku motorik. Berdasarkan hal tersebut, penelitian ini dilakukan untuk mendapatkan informasi penting tentang mekanisme antipenuaan otak, terutama tentang perbaikan histo-morfologi hipokampus dengan pemberian ekstrak ikan gabus (C. striata). Perbaikan wilayah otak ini secara otomatis akan memperbaiki kemampuan belajar-mengingat dan perilaku motorik. Ikan gabus yang digunakan dalam penelitian ini berasal dari Rawa Pening Kabupaten Semarang. Tikus-tikus percobaan didesain menggunakan rancangan pembandingan populasi melalui 2 tahap penelitian. Hewan uji yang digunakan adalah tikus Sprague dawley. Tahap pertama, populasi tikus percobaan yang berumur 4 bulan dengan penuaan fisiologis (tanpa perlakuan stres oksidatif) dibandingkan dengan populasi tikus percobaan yang mendapat perlakuan stres oksidatif setelah dua kelompok populasi tersebut diberi ekstrak ikan gabus. Tahap kedua, pembandingan kedua populasi dilakukan tanpa pemberian ekstrak ikan gabus. Ekstrak ikan gabus yang diberikan dengan dosis 4 ml/200 mg bb/hari. Hasil penelitian menunjukkan bahwa tikus dengan penuaan fisiologis memiliki profil histo-morfologi lebih baik dibanding tikus yang mengalami penuaan akibat stres oksidatif. Pemberian ekstrak ikan gabus terbukti dapat memperbaiki profil histo-morfologi hipokampus tikus pada kedua jenis penuaan dibanding tikus-tikus yang tidak mendapat perlakuan ekstrak ikan gabus. Simpulan penelitian ini adalah bahwa pemberian ekstrak ikan gabus (Channa striata) memiliki potensi sebagai antipenuaan dan mampu memperbaiki profil histo-morfologi hipokampus, baik pada penuaan fisiologis atau penuaan akibat stres oksidatif.   Keyword: Channia striata, antiaging, hipokampus, glutation, mitokondria, kemampuan  perilaku motorik
Efek Penambahan Virgin Coconut Oil (VCO) terhadap Perkembangan Jengger dan Bobot Testis Ayam (Gallus sp.) Santoso, Mohammad Sigit; Tana, Silvana; Mardiati, Siti Muflichatun
BULETIN ANATOMI DAN FISIOLOGI dh SELLULA Vol 18, No 1 (2010): Vol. XVIII, No. 1, Maret 2010
Publisher : BULETIN ANATOMI DAN FISIOLOGI dh SELLULA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (350.459 KB) | DOI: 10.14710/baf.v18i1.2601

Abstract

Chicken is one of food sources that useful for human as a source of animal’s protein. Chicken has to produce good quality of meat and egg. One way to increase the product of chick is through the management of chicken food by adding Virgin Coconut Oil (VCO). The advantage of VCO is known as a safe product for human, therefore it will be safe for chicken too, there no doubt to consume the meat.The purpose of this research is to know about the effect of VCO related to the development of comb and testis weight. The sample of this research are           20 leghorn rooster. The method of this research is random sampling with four treatment and five repetition (Po: 0 cc/kg,   P1 : 1,5 cc/kg, P2 : 3 cc/kg, and P3 : 6 cc/kg). The data is obtained by using ANOVA with 95% significance. The result of data showed that there are not real different about the development of comb and testis weight’s chicken. It shows that the concentration of using VCO as additif feed to increase the productivity not successful yet.
Respons Biologis Puyuh setelah Pemberian Cahaya Monokromatik: suatu Kajian Kualitas Telur Mardiati, Siti Muflichatun; Kasiyati, Kasiyati; Irawati, Fika; Silalahi, Adonia B
BULETIN ANATOMI DAN FISIOLOGI dh SELLULA Vol 19, No 1 (2011) : Volume XIX, Nomor 1, Maret 2011
Publisher : BULETIN ANATOMI DAN FISIOLOGI dh SELLULA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (372.715 KB) | DOI: 10.14710/baf.v19i1.2582

Abstract

Light schedule is one of improvement in avian culture. The objective of the research was evaluate the potential of monochromatic light used to source artificial light for increased the egg quality. One hundred and twenty quails (Coturnix coturnix japonica) were divided into four treatments of lights, with thirty quails in each treatments. The treatments were red, green, and blue lights with intensity of 15 lux. Control treatment used yellow incandescent bulb of 15 watt. The red, green, and blue lights were provided by light emitting diodes (LED). All lights treatment were given for 14 h daily, started from 17.00 to 07.00. The result showed that quails exposed to monochromatic light significant (P<005) in abdominal fat, the body weight, feed consumption, triglyseride concentration, and the fat content in egg, and the egg weight, without significant effect in the egg protein. Blue light could be used to alternative in quail culture for improve the egg quality
DIAMETER DAN TEBAL LAPISAN EPITEL TUBULUS SEMINIFERUS SERTA BOBOT TESTIS MENCIT (Mus musculus) SETELAH PEMBERIAN TAUGE KACANG HIJAU (Vigna radiata) Anindita, Reza; Djaelani, M. Anwar; Mardiati, Siti Muflichatun
BULETIN ANATOMI DAN FISIOLOGI dh SELLULA Vol 17, No 2 (2009): Volume XVII, Nomor 2, Oktober 2009
Publisher : BULETIN ANATOMI DAN FISIOLOGI dh SELLULA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (52.234 KB) | DOI: 10.14710/baf.v17i2.2562

Abstract

Young mung bean (Vigna radiata) believed as foodstuffs which fertility to defend potent on male. It reliable connected with to be vitamin E antioxidant essence which dominant by the side of other antioxidant as selenium and vitamin C which function in cell to protect from free radical reaction attack on spermatogenesis moment. Spermatogenesis activity on to occur testes inside at to direct seminiferous tubules. This experiment to aim learn tauge kacang hijau supply effect to be seminiferous tubules diameter, seminiferous tubules epithelium thick of layer and testes weight. Trial of animal which used is male rat to amount 10 tail, to divide appoint 2 group, Po as control and P1 is treatment which tauge kacang hijau to receive with oral dose 0,8 g/tail/day as long as 48 day. Observed which variable is seminiferous tubules diameter, seminiferous tubules epithelium thick of layer and testes weight. Acquired which data and then t-test using analyses with 95 % belief of level. Data analyses of result young mung bean supply effect to present not significanceof different to be seminiferous tubules diameter, seminiferous tubules epithelium thick of layer and testes weight treatment the mouse than control (P>0,05). Fact to present that young mung bean treatmen with most dominant vitamin E essence which antioksidant as function impotent spermatogenesis effect reliable in way.
Perbandingan Kadar Garam Natrium dan Kalium pada Tes Ferning Lendir Mulut Mardiati, Siti Muflichatun
JURNAL SAINS DAN MATEMATIKA Volume 15 Issue 1 Year 2007
Publisher : JURNAL SAINS DAN MATEMATIKA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (337.52 KB)

Abstract

ABSTRACT---The objective of this study was to know natrium and potassium levels of oral mucus ferning test. The object were 30 women, that were taken their oral mucus once within the 6th to 19th day of menstruation. The ferning test of oral mucus were examined with light microscope. The data distribution was analized by Kolmogorf – Smirnov, followed by non – parametric statistics. The mean, median and standard deviation were calculated with descriptive analysis. The Wilcoxon signed rank test was used to analyze the differentiation between the natrium and potassium levels of oral mucus in groups. The result of this study shows that the oral mucus potassium level is higher than natrium level. It means that potassium more important than natrium in the oral mucus ferning test.   Keywords : potassium, natrium, ferning score, oral mucus
Blood-Glucose Levels of Rats Given High-Fat Diets after Administration of Neem Leaf Ethanolic Extract Isdadiyanto, Sri; Mardiati, Siti Muflichatun; Sitasiwi, Agung Janika
Biosaintifika: Journal of Biology & Biology Education Vol 13, No 2 (2021): August 2021
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v13i2.29516

Abstract

Azadirachta indica A. Juss (Neem) is a medicinal plant that contains antioxidants, antihyperlipidemia, and has antidiabetic activity. This research aimed to examine the effect of neem leaf ethanolic extract on blood glucose levels of white rats given high-fat rations. The male white rats (Rattus norvegicus L.) with two months of age were used. The treatment of test animals consisted of six treatment groups and four replications, namely: the control group (P0); given high fat rations + duck egg yolk 2.5 ml/200gBW (P1); given high-fat rations + 8 mg/200gBW simvastatin (P2); and P1+ the dose of neem leaf ethanolic extract of 75; 100; and 125 mg/200gBW (P3; P4; and P5). Blood-glucose levels were analyzed by colorimetric enzymatic method using Roche Diagnostics Cobas C reagents kits. Blood-glucose level was determined using Roche / Hitachi cobas c systems automatically. Homogeneous and normally distributed data of blood-glucose levels, body weight and feed consumption were analyzed statistically using ANOVA followed by Duncan's test with a significance level of 5% using SPSS 16.0 software. The results indicated that giving the neem leaf ethanolic extract of could reduce the blood glucose levels. In this study, it was concluded that the neem leaf ethanolic extract of could potentially be used as an alternative to cure diabetes mellitus.
Efek Ekstrak Air Biji Pepaya (Carica papaya L.) terhadap Fertilitas Mencit (Mus musculus L.) Betina Muhammad Feraldi Firdaus; Agung Janika Sitasiwi; Siti Muflichatun Mardiati
Jurnal Sain Veteriner Vol 35, No 2 (2017): Desember
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (8099.065 KB) | DOI: 10.22146/jsv.34688

Abstract

Papaya seeds were known as medicinal plant which have anti-fertility potencies. So it could be used as a herbal contraceptive. The purpose of this study was to test the anti-fertility effect of Papaya seed water extract on mice (Mus musculus L.) fertility which proved by pregnancy rate, litters number and sex ratio of litters. This study was designed by Completely Randomized Design, using 15 female Swiss Webster mice that were divided into 5 treatment groups with triplicates. The K(-) group were treated by distilled water; K(+) group were treated by contraceptive pills; P1, P2, P3 groups that were treated by papaya seeds extract with 1.4, 3.5 and 7 mg/animal/day respectively. Each treatment was given orally with 0.5 ml in volume for 21 days. At the end of treatment, mice was mated. The observed parameters were pregnancies occurence, litters number and sex ratio of the litters. The litters number data were analyzed by Analysis of Variance. The results showed there was differences in pregnancy time and sex ratio between control and treatment groups, but there was not significantly different in litters number. It could be concluded that the fertility of mice was inihibited by water extract of papaya seeds.
Co-Authors Adonia B Silalahi Agung Janika Sitasiwi Agung Janika Sitasiwi Alyda Aliyah Rahmah Anggi Widyaningsih Anis Alfiyanti Beta Cahyaning Nugraheni Budianto, Emilia Yosephine Chatarina Umbul Wahyuni Desly Desly, Desly Dyah Ayu Maharani Putri, Dyah Ayu Maharani Eka Wahyu Cahya Ningrum Endang Nawarikita Erik Setiawan Fani Fahriyansyah Febry Nurmala Yuriwati, Febry Nurmala Fika Irawati Ibnu Thoriq Hidayat Isabella Vanny Primadiani, Isabella Vanny Kasiyati Kasiati Koen Praseno Koen Praseno Lia Lestari M. Anwar Djaelani Maharani, Wulandari Putri Martha Dian Indriati, Martha Dian Melati, Alfriyan Krisna Mohammad Sigit Santoso Muhammad Anwar Djaelani Muhammad Feraldi Firdaus Nunuk Shofiati Nur Azizah Rahita Dayurani Rani Wahyuninghasti Rohman, Jihadul Hanif Fadlur Rully Rahadian Silvana Tana Silvana Tana Sri Isdadiyanto Sri Isdadiyanto SRI ISDADIYANTO Sunarno Sunarno Sunarno Sunarno Teguh Suprihatin Tyas Rini Saraswati Tyas Rini Saraswati Vina Aviati Wicaksono, Wisnu Adi Wuri Widyastuti Yeni Alfiyah, Yeni Yuli Tri Utami, Yuli Tri Zuni Novika