Adioro Soetojo
Department Of Conservative Dentistry, Faculty Of Dental Medicine, Universitas Airlangga

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Differences in surface roughness of nanohybrid composites immersed in varying concentrations of citric acid Gabriela Kevina Alifen; Adioro Soetojo; Widya Saraswati
Dental Journal (Majalah Kedokteran Gigi) Vol. 50 No. 2 (2017): June 2017
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (326.137 KB) | DOI: 10.20473/j.djmkg.v50.i2.p102-105

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Background: The surface roughness of restoration is important in predicting the length of time it might remain in the mouth. Conditions within the oral cavity can affect the surface roughness of a restoration. Nanohybrid composite is widely used in dentistry because it can be applied to restore anterior and posterior teeth. Athletes routinely consume isotonic drinks which are acidic and even more erosive than the carbonated variety because they contain a range of acids; the highest content of which being citric acid. Purpose: The aim of the study was to analyze the surface roughness of nanohybrid composite after having been subjected to immersion in varying concentrations of citric acid. Methods: Two isotonic drinks (Pocari Sweat and Mizone) were analyzed using high performance liquid chromatography (HPLC) to quantify the respective concentrations of citric acid which they contained. A total of 27 samples of cylindrical nanohybrid composite were prepared before being divided into three groups. In Group 1, samples were immersed in citric acid solution derived from Pocari Sweat. Those of Group 2 were immersed in citric acid solution derived from Mizone; while Group 3, samples were immersed in distilled water as a control. All samples were immersed for 7 days, before their surface roughness was tested by means of a surface roughness tester (Mitutoyo SJ-201). Data was analyzed using a one-way ANOVA test. Results: The results showed that there was no significant difference in surface roughness between Groups 1, 2 and 3 (p=0.985). Conclusion: No difference in surface roughness of nanohybrid composites results from prolonged immersion in varying concentrations of citric acid.
The difference between residual monomer dentin bonding HEMA and UDMA with acetone and ethanol solvent after binding to type I collagen N. Normayanti; Adioro Soetojo; Nirawati Pribadi
Dental Journal (Majalah Kedokteran Gigi) Vol. 51 No. 4 (2018): December 2018
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (208.553 KB) | DOI: 10.20473/j.djmkg.v51.i4.p169-172

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Background: In caries and non-caries lesions involving dentine, it is necessary to provide dentine-bonding material to help improve retention between the composite resin and the tooth surface. Composite resin attachment to dentine is influenced by bonding polymerization reactions. In several studies, researchers found that polymerized monomers will experience volume shrinkage because not all will fully polymerize but, rather, become residual monomers that can cause post-operative pain. Purpose: This study aimed to identify the difference in the amount of residual monomers between HEMA- and UDMA-based dentin bonding materials with acetone and ethanol solvents after binding to type I collagen. Methods: Four groups featured in this study: HEMA with acetone solvent and type I collagen , HEMA with ethanol solvent and type I collagen , UDMA with acetone solvent and type I collagen and UDMA with ethanol solvent and type I collagen . All groups were checked by high performance liquid chromatography (HPLC) to quantify the remaining amount of monomers. Results: The percentage of residual monomers of dentine bonding HEMA with acetone solvent and type I collagen was 10.69%, HEMA with ethanol solvent and type I collagen was 13.93%, UDMA with acetone solvent and type I collagen was 2.89% and UDMA with ethanol solvent and type I collagen was 7.48%. Conclusion: HEMA with ethanol solvent has the highest number of residual monomers, while UDMA with acetone solvent has the lowest.
The effect of UDMA and Bis-GMA irradiation period on residual monomers in resin packable composite Jayanti Rosha; Adioro Soetojo; Putu Dewi Purnama Sari Budha; M. Mudjiono
Dental Journal (Majalah Kedokteran Gigi) Vol. 52 No. 1 (2019): March 2019
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (146.726 KB) | DOI: 10.20473/j.djmkg.v52.i1.p24-26

Abstract

Background: Residual monomers are non-polymerized monomers which can cause clinical harm, for example inflammation, to oral cavity tissue while the remaining monomers can potentially be carcinogenic. The more residual monomers that remain due to an imperfect polymerization processes, the lower the compressive strength level and the higher the number of micro slits that can cause secondary caries and tooth sensitivity. Urethane dimethacrylate (UDMA) and bisphenol A glycol dimethacrylate (Bis-GMA) constitute two of the resins most frequently used in packable composites. During the short irradiaton period forming part of the polymerization process, UDMA and Bis-GMA have the potential to produce residual monomers. Purpose: This study aimed to compare the number of residual monomers in packable composite resin following irradiation lasting 1x20 seconds and 2x20 seconds. Methods: 28 samples of cylindrical packable composite with a thickness of 2 mm and a diameter of 5 mm were divided into four groups. Groups 1 and 2 were irradiated for 1x20 seconds, and groups 3 and 4 for 2x20 seconds with the composite subsequently being immersed in ethanol solution for 24 hours. The number of residual monomers using high-performance liquid chromatography (HPLC) devices was calculated and the results statistically analyzed using a Mann-Whitney Test. Results: Repeated irradiation had no effect on the amount of residual monomers in packable composite resins. However, there were differences in the number of residual monomers in the material contained in packable composite resins Bis-GMA and UDMA, while the remaining monomers in UDMA outnumbered those in Bis-GMA. Conclusion: The number of residual monomers in Bis-GMA is lower than in the remaining UDMA after 1x20 seconds irradiation, while the number of residual monomers in Bis-GMA and UDMA following 2x20 seconds irradiation was no different to that after irradiation of 1x20 seconds duration.
Microleakage difference between total-etch and self-etch bonding in bulk fill packable composite restoration after carbonic acid immersion Widya Saraswati; Dian Pramita Ayu Kumalasari; Adioro Soetojo
Dental Journal (Majalah Kedokteran Gigi) Vol. 52 No. 4 (2019): December 2019
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/j.djmkg.v52.i4.p192-196

Abstract

Background: Bulk fill packable composite that can be applied to a depth of 4mm in cavities is widely used in posterior teeth restoration. Unfortunately, this composite is subject to potential microleakage which occurs due to erosion resulting from the consumption of carbonated drinks containing carbonic acid. Nevertheless, microleakage can be reduced by bonding applications the etch technique of which is divided into two forms; self-etch bonding and total-etch bonding. Purpose: This study aims to determine the difference in microleakage between total-etch and self-etch bonding in bulk fill packable composite following carbonic acid immersion. Methods: This study constitutes experimental laboratory research utilizing 28 incisors bovine teeth which were cleaned, immersed in 0.01% NaCl, and randomly divided into four groups. The cervical area of the teeth of all groups were prepared through the creation of cylindrical shapes 2mm in diameter and 3 mm deep. Groups I and III used total-etch bonding, while groups II and IV used self-etch bonding. Groups III and IV were control groups, whereas groups I and II were treatment groups (immersed in carbonic acid) for 24 hours. Thermocycling was carried out in all groups which were subsequently immersed in 1% methylene blue for 24 hours after which the teeth were cut in a buccolingual direction using a diamond disc wheel. Microleakage was subsequently evaluated by calculating the amount of methylene blue passing between the restoration wall and cavity using a Stereomicroscope and scanning electron microscope (SEM). The data was then analyzed using Kruskal Wallis and Mann Whitney U tests. Results: There was a significant difference between the control groups and treatment groups (p<0.05). The microleakage in Group 2 was higher than that of other groups. Conclusion: The microleakage of total-etch bonding was lower than self-etch bonding in bulk fill packable composite after carbonic acid immersion.
PERBEDAAN KEKERASAN PERMUKAAN ENAMEL SETELAH APLIKASI FLUORIDE VARNISH DAN CASEIN PHOSPO PEPTIDE-AMORPHOUS CALSIUM PHOSPHATE FLUORIDE (CPP-ACPF) (PENELITIAN IN VITRO) Sinta Puspita; Adioro Soetojo; Sri Kunarti
Conservative Dentistry Journal Vol. 7 No. 2 (2017): July - December
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (311.726 KB) | DOI: 10.20473/cdj.v7i2.2017.130-137

Abstract

Background: Caries is a chronic, slowly progressing disease, with symptoms not detected at the onset of the disease but generally much later. Its initiation is associated with demineralization (calcium and phosphate loss) of subsurface tooth enamel, resulting in the formation of a subsurface lesion. To restore the natural equilibrium, either remineralization must be enhanced or demineralization must be retarded. There are some topical agents that can enhance remineralization such as topical fluor and casein phosphopeptide – amorphous calcium phosphate (CPP-ACP). Purpose: The aim of this study is to analyze the differences of the enamel surface microhardness after application of fluoride varnish and CPP-ACPF. Methode: 27 blocks bovine enamel were devided into 3 groups. Group 1 – control (No surface treatment), group 2 – fluoride varnish and group 3 – CPP-ACPF. Initial surface hardness enamel was measured for all enamel specimens. Artificial enamel carious lesions were created by immersing enamel samples to demineralization solution (pH 4,5) for 72 hours at temperature 370 C. The surface microhardness of demineralized enamel specimens was measured. A caries progression test (pH cycling) was carried out, which consisted of alternative demineralization (3 hours), remineralization with artificial saliva (21 hours) and application topical agent twice a day for 14 days. Then, the last surface enamel microhardness is measured. Result: Group 3 showed significantly highest Vickers hardness number (P<0,05) followed by group 2 and the lowest is group 1. Conclusions: This study proved that enamel surface microhardness after application of CPP-ACPF was higher than fluoride varnish.
PERBEDAAN KEKUATAN IKATAN KIMIA ANTARA BAHAN BONDING HEMA DENGAN PELARUT ASETON DENGAN BAHAN BONDING HEMA DENGAN PELARUT ETANOL PADA KOLAGEN Adioro Soetojo; Ivan Salomo Sumarto; Moch. Mudjiono; Cecilia G.J. Lunardhi
Conservative Dentistry Journal Vol. 8 No. 2 (2018): July - December
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (259.801 KB) | DOI: 10.20473/cdj.v8i2.2018.69-76

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Latar belakang: Resin komposit berikatan dengan struktur gigi melalui bahan adesif, yang dikenal dengan sebutan bahan bonding. Bahan bonding biasanya mengandung monomer fungsional yang spesifik pada umumnya adalah ester yang berasal dari reaksi alkohol bivalen dengan asam metakrilat dan phosphor atau derivate asam karboksilat.dalam studi terbaru terdapat ikatan bahan bonding secara kimia. Tujuan: Untuk menganalisis perbedaan kekuatan ikatan kimia antara bahan resin bonding berbahan dasar HEMA berpelarut etanol dan HEMA berpelarut aseton dengan kolagen dentin. Metode: Terdapat 3 kelompok pada penelitian ini. Kelompok pertama HEMA dan kolagen dicampur dengan KBr, kelompok ini sebagai kontrol positif. Kelompok kedua HEMA, etanol, dan kolagen dicampur KBr. Kelompok ketiga HEMA, aseton, dan kolagen dicampur KBr. Ketiga kelompok kemudian dijadikan pellet bening dan dianalisa dengan FTIR,untuk kemudian dihitung nilai puncak pita serapan karbonil dari masing-masing kelompok penelitian. Data dianalisa menggunakan Kruskall Wallis dan Tukey HSD test (p<0.05). Hasil: Nilai Peak (P) pita serapan karbonil kelompok I: 71.36, kelompok II : 46.76, kelompok III : 27.2. Semakin kecil nilai Peak (P) pita serapan karbonil maka diamsusikan semakin besar kekuatan ikatan secara kimia. Kesimpulan: Kekuatan ikatan kimia pada bahan bonding HEMA berpelarut aseton lebih besar dibandingkan dengan berpelarut etanol pada kolagen dentin.
Ekspresi MMP-1 dan TGF-β1 setelah aplikasi dentin bonding berbasis HEMA Adioro Soetojo; Mulia Rahmah; Ari Subiyanto; Moch. Mudjiono
Conservative Dentistry Journal Vol. 8 No. 2 (2018): July - December
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (472.424 KB) | DOI: 10.20473/cdj.v8i2.2018.77-84

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Background: Dentin bonding adalah bahan untuk melekatkan resin komposit terhadap dentin. Komponen utama penyusun dentin bonding adalah 2-hydroxyethyl methacrilate (HEMA). Setelah polimerisasi, terdapat monomer sisa yang akan berdifusi ke dalam tubuli dentin sampai ke dalam pulpa yang dapat mempengaruhi sel odontoblas. Objective: Menganalisis ekspresi MMP-1 dan TGF-β1 pada dentin gigi tikus yang diberikan dentin bonding HEMA dan HEMA murni. Methods: Gigi molar pertama rahang atas tikus wistar dipreparasi klas 1 tanpa aplikasi bahan selama 3 hari (kelompok 1), kemudian diaplikasikan HEMA murni selama 3 hari (kelompok 2), dentin bonding HEMA selama 3 hari (kelompok 3). Kemudian gigi ditumpat dengan Fuji II LC. Tikus wistar di dekaputasi dan rahangnya direseksi pada hari ke 3 kemudian diproses untuk pemeriksaan histokimia. Result: Ekspresi MMP-1 pada penggunaan dentin bonding HEMA lebih sedikit dibandingkan dengan HEMA murni,  ekspresi  TGF-β1 pada penggunaan dentin bonding lebih banyak dibandingkan dengan HEMA murni. Conclusion : Ada perbedaan bermakna pada ekspresi MMP-1 dan TGF-β1 antara penggunaan dentin bonding HEMA dibandingkan dengan HEMA murni
Sitotoksisitas Ekstrak Kulit Kakao (Theobroma cacao) terhadap Kultur Sel Fibroblas BHK-21 Fajariana Fitriani; Adioro Soetojo; Agus Subiwahjudi; tamara yuanita
Conservative Dentistry Journal Vol. 9 No. 1 (2019): January - June
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (621.928 KB) | DOI: 10.20473/cdj.v9i1.2019.54-65

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Latar Belakang: Irigasi saluran akar merupakan salah satu tahapan penting untuk menunjang keberhasilan perawatan. Sodium hipoklorit (NaOCl) merupakan larutan irigasi utama yang sering digunakan namun memiliki sejumlah kekurangan yakni bersifat toksik jika diirigasi sampai ke jaringan periradikular. Kakao (Theobroma cacao) merupakan tumbuhan suku Sterculiaeae yang kulit buahnya mengandung senyawa alkaloid, flavonoid, tanin, saponin dan terpenoid. Senyawa-senyawa tersebut terbukti dapat menghambat pembentukan biofilm pada bakteri Enterococcus faecalis yang merupakan bakteri paling resisten pada saluran akar. Ekstrak kulit kakao diharapkan dapat menjadi bahan alternatif irigasi saluran akar yang ideal, sehingga perlu dilakukan penelitian mengenai efek sitotoksisitasnya terhadap jaringan. Tujuan: Menentukan konsentrasi dari ekstrak kulit kakao yang memberikan efek sitotoksik pada sel fibroblas BHK-21. Metode: Jenis penelitian yang dilakukan adalah eksperimental laboratoris dengan menggunakan kultur sel fibroblas BHK-21. Ekstrak kulit kakao diperoleh melalui maserasi menggunakan etanol 70% dan dilakukan pengenceran dengan konsentrasi 1,56%, 3,125%, 6,125%, 12,5%, 25%, 50%, dan 100%. Sel BHK-21 dalam microplate 96 well dipaparkan dengan ekstrak kulit kakao. Uji sitotoksisitas menggunakan MTT assay dan absorbansi warna dibaca menggunakan Elisa reader. Nilai absorbansi dihitung dengan rumus sehingga didapatkan hasil akhir berupa persentase kematian sel. Hasil: Peningkatan konsentrasi ekstrak kulit kakao berbanding lurus dengan kenaikan persentase sel fibroblas BHK-21 yang mati. Kesimpulan: Konsentrasi minimum ekstrak kulit kakao yang dapat memberikan efek sitotoksik pada sel fibroblas BHK-21 adalah 6,25%.
Management Of White Spot And Central Diastema On Anterior Teeth With Direct Composites Restoration (A Case Report) Adioro Soetojo; Bintang Adiguna Widjaja
Conservative Dentistry Journal Vol. 9 No. 2 (2019): July - December
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (586.267 KB) | DOI: 10.20473/cdj.v9i2.2019.66-69

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Background: One of the most common aesthetic problems is discoloration and central diastema of the anterior teeth. This can make the patient's appearance disturbed and can lead to confidence in influential patients in the complex interaction of social, cultural and psychological disorders. Purpose: The purpose of this case report is to explain the management of white spot on central incisive with central diastema. Case: A 22 years old woman came to Airlangga Dental Hospital with a chief complaint of shyness and lack of confidence due to some white spot and gap on the two central front teeth. Management: The treatment of white spot and central diastema were done using a partial direct veneer technique. Conclusion: White spot and central diastema treated with partial direct veneer restoration is able to produce good aesthetic results.
The effectivity of cavity cleanser chlorhexidinegluconate 2% and saponin 0.78% of mangoosteen peel Maretha Siwinata; Nanik Zubaidah; Adioro Soetojo
Conservative Dentistry Journal Vol. 10 No. 1 (2020): January-June
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (438.248 KB) | DOI: 10.20473/cdj.v10i1.2020.19-22

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Background. Use of cavity cleanser is important before restoration the teeth to clean debris, residue of prepared dentine, blood, bacteria, collagen denaturized by teeth preparation. Nowadays, the cavity cleanser that people used still having shortcoming, one of which is the lack of ability to clean the root canal of the smear layer. Purpose. The purpose of this study examines the difference of cavity cleaner between saponin of mangoosteen peel (GarciniaMangostana L.) and chlorhexidinegluconate 2%. Method. Eighteen upper first premolar divided into 3 groups, each of them consist three tooth. Forming the preparation tooth cavity then group 1 using aquadest for control group, group 2 using chlorhexidinegluconate 2%, and group 3 using saponin of mangoosteen peel (GarciniaMangostana L.). For rating cleanliness of the tooth cavity using a scale of cleanliness conducted under Scanning Electron Microscope. Result. There was a significant difference (p < 0.05) in One-Way ANOVA parametric test andpos hoc test between chlorhexidinegluconate 2 % and saponin of mangoosteen peel to the cleanliness of the tooth cavity. Conclusion.According to the result of the study, it can be concluded that saponin of mangoosteen peel (GarciniaMangostana L.) less effective for cleaning the tooth cavity than chlorhexidinegluconate 2%.