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All Journal Dentika Dental Journal Dental Journal (Majalah Kedokteran Gigi) Conservative Dentistry Journal Indian Journal of Forensic Medicine & Toxicology

Kun Ismiyatin

Department Of Conservative Dentistry, Faculty Of Dental Medicine, Universitas Airlangga

Author-ID : 3196485

Dentistry Public Health

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EFFECTIVENESS OF TIME IRRADIATION BY LASER DIODE 650 NM AND PHOTOSENSITIZER Methylene Blue ON DECREASING THE NUMBER OF MIXED BACTERIAL COLONIES FROM NECROTIC ROOT CANAL Nadya Ayusandra Larasati; Kun Ismiyatin; Sri Kunarti
Conservative Dentistry Journal Vol. 9 No. 1 (2019): January - June
Publisher : Universitas Airlangga

Background. Pulp necrosis is defined as the irreversible death of pulp tissue. It clinically observed by the destruction of its own tissue. The primary etiology of pulp necrosis is irritation due to bacterial infection. The treatment for pulp necrosis is root canal treatment with a success percentage ranging from 40-93% based on cavities with minimal bacteria that can be sterilized. One method of sterilization using Antibacterial Photodynamic Therapy (aPDT), which uses laser diodes is currently being developed in the field of conservative dentistry, but there are still many differences of opinion regarding the ideal amount of time of laser radiation or photoactivation used to reduce the number of bacteria, especially in root canals. Aim. To determine the effective radiation time of the 650 nm wavelength laser diode with Methylene Blue Photosensitizer in reducing the number of bacterial colonies from necrotic root canals. Method. This research is a laboratory experimental study with 30 samples and 6 groups with different durations of irradiation namely 45, 60, 75 and 90 seconds. Results. It was found that there was a decrease in the number of mixed bacterial colonies within necrotic root canals with the obtained p-value of the ANOVA test results being <0.05. This shows that there is a significant difference between the amounts of mixed bacterial colonies from necrotic root canals in each treatment group. Conclusion. Diode laser radiation with a wavelength of 650 nm with duration of 90 seconds of radiation and Methylene Blue Photosensitizer is an effective time in reducing the number of bacterial colonies from necrotic teeth compared to the duration of radiation of 45, 60 and 75 seconds.

Effect of 405 nm Diode Laser with Varying Irradiation Time on BHK-21 Fibroblast Viability Kun Ismiyatin; Leidy Herlin Rumbiak; Widya Saraswati; Sri Kunarti; Anuj Bhardwaj
Conservative Dentistry Journal Vol. 9 No. 1 (2019): January - June
Publisher : Universitas Airlangga

Background: Laser is a device that emits light through a process of optical amplification based on the stimulated emission of electromagnetic radiation; it can be used for surgery, detoxification, bio stimulation and antibacterial. However, lasers have Biphasic Dose Response (BDR), which is bio stimulation and bio inhibition. To determine if 405 nm laser diode is biocompatible, viability test is necessary before these lasers can be labeled as viable to use in dental therapy. Aim: To prove the variation of radiation time of the 405 nm laser diode radiation can cause bio stimulation and bio inhibition response that affects the viability of BHK-21 fibroblast cells. Method: Viability test was carried out using BHK-21 fibroblast cells which were inserted into 96-well microplate, then radiated with 405 nm laser diode with varying irradiation time of 30s, 60s, 120s, 240s and 480s. After radiation, the cells are then incubated for 24h. Cytotoxicity was observed using MTT assay and ELISA reader. Data was analyzed using the Kolmogorov-Smirnov test, Levene Test, Welch ANOVA, and Tukey HSD. Results: BHK-21 fibroblast cells radiated with 405 nm laser diode with radiation time of 30s, 60s, 120s, and 240s have the same viability as the control cell, while at 480 seconds the viability exceeds that of the control cell. Conclusion: 405 nm laser diode with radiation times of 30s, 60s, 120s, and 240s do not affect the viability of BHK-21 fibroblast cells. Meanwhile, 480s irradiation time of 405 nm laser diode causes bio stimulation response that increases the viability of BHK-21 fibroblast cells.

Effect of Hydrogel Epigallocatechin-3-Gallate (EGCG) to the Number of Fibroblast Cell Proliferation in the Perforation of Wistar Rat Tooth Pulp Ira Widjiastuti; Setyabudi setyabudi; Kun Ismiyatin; Djoko Agus Purwanto; Tiara Sukmawati
Conservative Dentistry Journal Vol. 9 No. 2 (2019): July - December
Publisher : Universitas Airlangga

Background: Pulpitis can occur because the deep cavity preparation and it causes increasing of NO levels. Perforated teeth require direct pulp capping (DPC) treatment. The current standard DPC material is calcium hydroxide. However, several studies have found weaknesses of calcium hydroxide that can affect the success of DPC treatment and new, more biocompatible materials are needed. Epigallocatechin-3-gallate (EGCG) in green tea has many benefits, including antioxidant, anticolagenase, anticancer, anti-inflammatory and has the ability of radical scavenging to clean NO so that pulp healing can occur better by increasing the number of fibroblast cells that play a role in wound healing. Purpose: To determine the concentration of hydrogel EGCGs that are effective in increasing the number of fibroblast cell proliferation in the dental pulp perforation of Wistar rats. Method: This research is a laboratory experimental study with a randomized post test only control group design. Samples used in the study were 24 male Wistar rats which were divided into four groups, namely the negative control group and the treatment group were given EGCG 60 ppm, 90 ppm, and 120 ppm and were decapitated on the 7th day after treatment. The maxilla and the 1st molar were taken and decalcified, to process the HPA reading with HE staining. Observations were made using a microscope with a magnification of 400x. Results: There were significant differences in the treatment groups with 60 ppm and 90 ppm hydrogel hydrogels on the results of the Oneway ANOVA difference test (p <0.05). Conclusion: The concentration of hydrogel EGCG which is effective in increasing fibroblast cell proliferation is 90 ppm.

Effect of Epigallocatechin-3-Gallate (EGCG) on The Number of Lymphocyte Cells in Inflammation of Pulp with Mechanical Injury Kun Ismiyatin; Latief Mooduto; Putri Dea Amani Faadhilah
Conservative Dentistry Journal Vol. 10 No. 1 (2020): January-June
Publisher : Universitas Airlangga

Background: Pulpitis is an inflammatory pulp that can caused by pulp perforation by mechanical injury. Emergency treatment of pulpitis is using Eugenol. High concentrations of Eugenol have a cytotoxic effect that causes local necrosis and inhibits the healing process. Because of negative effects from eugenol, then it’s necessary to consider a new ingredient with minimal side effects, and it’s epigallocatechin-3-gallate (EGCG) in green tea. As a polyphenol, it has good antioxidant effect and plays a role in shortening the duration of inflammation by radical scavenging against Nitric Oxide so that NO levels rapidly decrease which causes migration of neutrophil cells to the area of injury will decrease and the inflammatory process faster so that the healing process become faster. Lymphocyte is plays a role in tissue repair. Purpose: to explain the effect of EGCG hydrogel on the number of lymphocyte cells in pulp inflammation with mechanical injury. Methods: The study used 24 Wistar rats divided into four groups, namely control (C), and 3 groups of treatment (T1, T2, T3). Each group consisted of 6 rats prepared, then EGCG hydrogel 60 ppm was named T1, EGCG hydrogel 90 ppm was named T2, EGCG hydrogel 120 ppm was named P3. On the 3rd day, Wistar rats were decapitated for HPA preparations. Results: This study showed a significant difference in each group (p < 0.05) using One-Way Anova analysis. Conclusion: EGCG hydrogel 90 ppm is effective in increasing the number of lymphocyte cells in inflammation of the pulp with mechanical injury.

Effect Of Epigallocatechin-3-Gallate (EGCG) On The Number Of Macrophage Cells In Inflammation Of Pulp With Mechanical Injury Kun Ismiyatin; Devi Eka Juniarti; Djoko Agus Purwanto; Adecha Ekalipta Primazafira
Conservative Dentistry Journal Vol. 10 No. 1 (2020): January-June
Publisher : Universitas Airlangga

Background: Pulpitis is an inflammatory pulp that can occur due to mechanical trauma that causes perforation of the pulp. Treatment of pulpitis Emergency frequently using Eugenol. High concentrations of Eugenol have a cytotoxic effect that causes local necrosis and inhibits the recovery process. An alternative consideration with Epigallocatechin 3-gallate (EGCG) which has good antioxidant properties and increases the complexity of inflammation by inhibiting the production of inducible nitric oxide synthase (iNOS) and nitric oxide so that levels migration of inflammatory cells to the area of injury will decrease and inflammation will occur shorter then initiates the proliferation so the recovery process and tissue repair will be more rapidly occurring. One of the cells that support tissue repair is macrophages. Purpose: this study aims to explain the effect of EGCG on the number of macrophage cells in pulp inflammation with mechanical injury. Methods: The study used 24 Wistar rats teeth divided into four groups, namely control (C), and 3 treatment groups T1, T2, and T3. Each group consisted of 6 rats prepared, then T1 was named EGCG hydrogel 60 ppm, T2 was given EGCG hydrogel 90 ppm, while T3 was given EGCG hydrogel 120 ppm. On the 3rd day, Wistar rats were decapitated to continue making HPA preparations. Results:This study showed a significant difference in each group (p< 0.05) using One-Way Anova analysis. Conclusion: EGCG hydrogel 90 ppm is effectivein increasing the number of macrophage cells.

Antioxidant Potential of Epigallocatechin-3-gallate, Ascorbic Acid, and Sodium Ascorbate in Solution and Gel Forms by 2,2-diphenyl-1-picrylhydrazyl (DPPH) Assay Virdah Dwi Dewaantari; Setyabudi Setyabudi; Kun Ismiyatin
Conservative Dentistry Journal Vol. 11 No. 1 (2021): January-June
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/cdj.v11i1.2021.19-23

Background: Free radicals are molecules without any electron pairs, unstable, and highly reactive. Antioxidants are needed to reduce free radicals. Antioxidants provide various benefits in dentistry as a preventive agent for caries, healing, bone formation, mouthwash, preventive and therapeutic cancer, and reduction of periodontal disease progressions. Additionally, research on antioxidants is still undergone due to the existence of free radical residues on bleached teeth. Epigallocatechin-3-gallate (EGCG), ascorbic acid (AA), and sodium ascorbate (SA) are ingridients that have antioxidant properties. Antioxidants can be in two forms solution and gel. Solutions have a higher substance releasing power than gel. Gel is extremely adhesive, so it does not flow easily. Meanwhile, solutions are rather unstable because it flows easily. Antioxidant activities were evaluated with 2,2-diphenyl-1-picrylhydrazyl (DPPH) Assay method. Purpose: Analyzing through literature reviews the potential antioxidants of EGCG, AA, and SA in solution and gel forms by DPPH Assay. Reviews: There were eight journal articles used in this review. The first article described antioxidant solutions in which EGCG was higher than sodium ascorbate. The second article showed that antioxidant gel EGCG was lower than sodium ascorbate. The third journal explained that ascorbic acid was higher than sodium ascorbate both in solution and gel forms. The other articles provided some information about the antioxidant activity percentages of EGCG, SA, and AA in the forms of gel and solutions by DPPH assay. Conclusion: EGCG has a higher antioxidant activity than SA, but it is lower when compared to AA in both solution and gel forms by DPPH assay.

The Role of Epigallocathecin-3-gallate as an Antioxidant After Dental Bleaching on Shear Bond Strength of Composite Resin Restoration Syarifah Nadhira Assyafira Al-Habsyi; Kun Ismiyatin; Galih Sampoerno
Conservative Dentistry Journal Vol. 11 No. 1 (2021): January-June
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/cdj.v11i1.2021.42-45

Background: Tooth discoloration can be treated with dental bleaching using Hydrogen peroxide (H2O2). Dental bleaching may interfere with the shear bond strength of composite resins because the remaining free radicals can affect bonding polymerization. Epigallocatcehin-3-gallate (EGCG) as an antioxidant can neutralize the free radicals produced during bleaching process. Purpose: Analyze the role of EGCG antioxidants in increasing the shear bond strength of composite resin after bleaching.. Reviews: Of the seven journals included in this literature review, six journals reported significant difference, and one journal noted no significant difference in the shear bond strength of composite resin following the EGCG application. Conclusion: The use of EGCG can increase the shear bond strength value of post-bleaching composite resin restorations.

Zirconia Inlay on Vital Teeth with Profunda Caries Sandra Kartika Sari; Kun Ismiyatin; Bagus Aji Wibowo; Rara Amorita Miranda
Conservative Dentistry Journal Vol. 11 No. 2 (2021): July - December
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/cdj.v11i2.2021.72-78

Background: Dental caries has historically been considered the most critical component of the global burden of oral disease. Health facilities and dental health education counselling have been conducted, but public knowledge about dental caries is still low. The increasing number of dental caries is currently influenced by one of the factors of community behaviour. Most people do not realize the importance of taking care of oral and dental health. The ignorance of the community results in a decrease in productivity due to the influence of the perceived illness. Advances in dentistry since the last decade has allowed the use of conservative dental care. Modern restorative dentistry offers many methods for restoring teeth, both direct and indirect. The need for restoration of posterior teeth is related to aesthetic purposes and functional, biocompatibility and biomechanical aspects of the remaining tooth structure. Some materials that are widely used as tooth-coloured indirect restorations in posterior teeth are zirconia. Zirconia has its characteristics, especially in terms of functionality, such as mechanical strength, physical strength and aesthetics. Purpose: This study aims to determine the management of indirect restoration treatment using zirconia inlay on upper premolar. Case(s): A 46-yearold male patient complained that the filling of his upper left tooth was often loose and uncomfortable when used for eating because the food was stuck in it. The patient wants his teeth treated. The history of treatment on the tooth in question has been patched two times, but it often comes off partially. Case Management: From the examination that has been carried out, a clinical diagnosis of reversible pulpitis was established. The treatment plan that will be carried out is indirect pulpcapping using MTA and resin-modified glass ionomer cement as the base material. The planned restoration treatment is a fixed inlay restoration made of monolithic zirconia. Conclusion: Recently, zirconia has also been developed staining with improved translucency so that it becomes more aesthetic. Zirconia has a higher level of material resistance than otherrestorative materials such as composites. This is what makes zirconia the choice, especially for use as a framework for all-ceramic and partially-fixed crowns dental prosthesis.

The aesthetic management of diastema closure treatment with indirect veneer using lithium disilicate: A case report Kun Ismiyatin; Olivia Vivian Widjaja; Singgih Harseno; Ahmad Afif Dzulfiqar; Caeleb Fabrizio Sudarsono; Indira Moza Azzaria
Conservative Dentistry Journal Vol. 12 No. 1 (2022): January-June
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/cdj.v12i1.2022.1-5

Background: Diastema is a space between two or more adjacent teeth that can interfere with the patient's aesthetic appearance. Various aesthetic treatments can be performed for diastema closure on anterior teeth, one of which is through the installation of veneers. The lithium disilicate ceramic material produces the thinnest veneer and has better properties than other materials, and is able to cover the anterior diastema with a fairly large width. Purpose: To describe the aesthetic procedure for closing the diastema of anterior teeth using indirect lithium disilicate ceramic veneer. Case(s): A 29-years-old male patient came with a complaint of dissatisfaction with her smile because of a gap between teeth 12 and 13. The teeth are in vital condition with normal overjet and overbite, and there are no systemic health problems. Case Management: The management of diastema closure on teeth 12 and 13 was carried out with indirect lithium disilicate veneer treatment. Lithium disilicate veneer has promising aesthetic results due to its enamel-like nature, minimally invasive because it requires minimal tooth reduction, good strength, and did not cause gingival irritation. As a result, this treatment could increase self-confidence of the patient. Conclusion: Diastema closure treatment using indirect lithium disilicate veneer is an effective treatment, gives satisfactory results and can increase the patient's confidence.

Single visit root canal treatment with post crown restoration Ismiyatin, Kun; Saraswati, Widya; Cahyani, Febriastuti; Vivian Widjaya, Olivia; Ghozali Pangestu, Dede; Arshila, Dea
Conservative Dentistry Journal Vol. 12 No. 2 (2022): July-December
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/cdj.v12i2.2022.57-62

Background: Single visit endodontics has been shown to be an effective treatment modality when compared with multiple visit therapy and it does not deviate from achieving the objectives of proper biomechanical preparation, debridement, shaping, disinfection and 3 dimensional obturation of root canal system and is more beneficial to the patient and dentist provided there is careful case selection and strict follow of standard endodontic protocols. Symptomatic teeth can also be managed by single visit after controlling the abscess infection with antibiotic, provided the root canal is dry without any discharge.Purpose: This study aims to perform single visit root canals on teeth with irreversible pulpitis and restoration with post core and crown. Case(s): A 48-years-old female comes with dental complaints of upper left rear spontaneous pain at night. The tooth has had cavities since about two years ago and has never been previously treated. The patient said had not any systematic health disorder. There is a spontaneous pain that the patient feels. Case Management: From the examination that has been carried out, a clinical diagnosis of tooth 25 were symptomatic pulpitis irreversible with normal apical tissue. The restoration endodontic treatment plan was a single visit endodontic and the restoration is post core and crown. Conclusion: Symptomatic pulpitis irreversible with normal apical tissue treatment can be carried out single visit root canal treatment. Restoration of post endodontic treatment in this case need fiber post and core using composite and finally restoration using crown that made of zirconia. Zirconia has a higher level of material resistance than lithium disilicate. This is what makes zirconia the choice, especially for posterior teeth.

Co-Authors Adecha Ekalipta Primazafira Adioro Soetojo Agustina Restu Nurkhotimah Ahmad Afif Dzulfikar Ahmad Afif Dzulfiqar Anuj Bhardwaj Ari Subiyanto Arshila, Dea Azzaria, Indira Moza Bagus Aji Wibowo binti Tengku Ahmad Noor, Tengku Natasha Eleena Caeleb Fabrizio Sudarsono Chang, Nicole Ava Cinitra Anindya Daradjat, Kinanti Naianuha Devi Eka Juniarti Dian Agustin Wahjuningrum Dian Dwi Pratiwi Djoko Agus Purwanto Dzulfiqar, Ahmad Afif Eric Priyo Prasetyo Febriastuti Cahyani Galih Sampoerno Galih Sampoerno Ghozali Pangestu, Dede Goenharto, Setyabudi Gusniarti, Haninda Dhipta Halima, Siti Noorraida Handoko, Jessica Cristy Harseno, Singgih Hendri Eko Wahyudi Hendy Jaya Kurniawan Ika Tangdan Indira Moza Azzaria Ira Widjiastuti Karina Awanis Adla Karina Erda Saninggar Kezia Sepdwiningtyas Santoso Latief Mooduto, Latief Leidy Herlin Rumbiak Michelle Suhartono Nadya Ayusandra Larasati Nanik Zubaidah Nindhira Puspita Sari Nirawati Pribadi Nirawati Pribadi Nurkhalidah, Binar Najwa Olivia Vivian Widjaja Olivia Vivian Widjaja Paramita Tanjung Sari Pramesty, Azzahra Kinaya Pramita Tanjung Sari Putri Dea Amani Faadhilah Raftiani, Ardelia Sabrina Rahmi Nawawi Rara Amorita Miranda Reinold C. Lina Ria Puspita Sari Ria Puspita Sari Rizky Ernawati Salicha, Ramadhani Putri Sandra Kartika Sari Sandra Kartika Sari Saptaningrum, Mardikaning Riasta Setyabudi Setyabudi Setyabudi Singgih Harseno Singgih Harseno Sri Kunarti Sri Kunarti Sri Kunarti, Sri Sudarsono, Caeleb Fabrizio Supandji, Rafaelle Raditya Syarifah Nadhira Assyafira Al-Habsyi Sylvia Tiara Sukmawati Virdah Dwi Dewaantari Vivian Widjaya, Olivia Widjaja, Olivia Vivian Widya Saraswati

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Journal : Conservative Dentistry Journal