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All Journal Dental Journal (Majalah Kedokteran Gigi) Conservative Dentistry Journal
Sri Kunarti
Department Of Conservative Dentistry, Faculty Of Dental Medicine, Universitas Airlangga
Dentistry

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Viability Test of Photodynamic Therapy with Diode Laser Waves Length 405 nm on BHK-21 Fibroblast Cells with Various Irradiation Distances Sri Kunarti; Vina Zavira Nizar; Widya Saraswati
Conservative Dentistry Journal Vol. 9 No. 2 (2019): July - December
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (487.74 KB) | DOI: 10.20473/cdj.v9i2.2019.82-86

Abstract

Background: Photodynamic therapy has now become popular, but its cytotoxic effect is still unclear. In order to be considered suitable for oral cavity therapy, the therapy must not be toxic or cause adverse effects on the target tissue. Viability testing for photodynamic therapy is important to do. Fibroblast cells are often used for testing the toxicity of dentistry because they are the most important cells in the components of the pulp, periodontal ligament, and gingiva. Purpose: To prove the effect of irradiation distance on photodynamic therapy on the viability of BHK-21 fibroblast cells. Method: Viability test was performed with BHK-21 fibroblast cells placed on a 96 well microplate which was then irradiated with 405 nm photodynamic therapy with varying irradiation distances of 1, 4, 7, 10, 13, and 16 mm. After irradiation, cell viability was tested by MTT assay and ELISA Reader. Data were analyzed using Kolmogorov-Smirnov, Levene’s test, Kruskall Wallis, and Tukey HSD. Result: Fibroblast cells with 4 mm irradiation distance have viability over control cells, whereas at irradiation distances 1, 7, 10, 13, and 16 mm have less viability than control cells. Conclusion: Photodynamic therapy 405 nm with 4 mm irradiation distance gives a biostimulation response so that the viability of BHK-21 fibroblast cells increases.
The Duration Effectivity of Diode Laser 405 nm with Erythrosine Photosensitizer in Killing Streptococcus Mutans Sri Kunarti; Anin Dita; Widya Saraswati
Conservative Dentistry Journal Vol. 10 No. 1 (2020): January-June
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (441.481 KB) | DOI: 10.20473/cdj.v10i1.2020.1-4

Abstract

Introduction: Dental caries is a multifactorial disease caused by the interaction of pathogenic bacteria, especially Streptococcus mutans. Various caries prevention chemically and mechanically can help reduce the number of bacteria. However, this approach does not cover the entire bacterial population with a photodynamic therapy approach with the addition of photosensitizers and low-intensity light radiation with the right wavelength able to eliminate the number of S. mutans bacteria. Purpose:To determinethe duration of effective irradiation time on a 405 nm diode laser with erythrosine photosensitizer in killing S. mutans bacteria.Methods: This research was an experimental laboratory type with 25 S. mutans samples divided into 5 groups treated with erythrosine photosensitizer 0.1 mg / ml and 405 nm diode laser irradiation with 45 seconds, 60 seconds, 75 seconds duration and no erythrosine photosensitizers and without irradiation. The growth of S. mutans bacteria was calculated then the data were analyzed statistically.Results:the research found that S. mutans bacteria decreased gradually in each group; erythrosine without photosensitizer and without irradiation had the highest average growth rate of 71.6 CFU / ml; without photosensitizer with irradiation of 40.6 CFU / ml; irradiation with photosensitizer 45 seconds at 20.6 CFU / ml; irradiation with 60 seconds photosensitizer at 11 CFU / ml; and irradiation with 75 seconds photosensitizer at 0 CFU / ml. In statistical tests, the data are normally distributed and homogeneous. There are significant differences between groups.Conclusion: The effective duration in this study was found at 75 seconds.
Antibiofilm Activity of Mangosteen (Garcinia mangostana L.) Flavonoids against Streptococcus mutans Bacteria Sri Kunarti; Aulia Ramadhani; Laskmiari Setyowati
Conservative Dentistry Journal Vol. 10 No. 2 (2020): July-December
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/cdj.v10i2.2020.48-50

Abstract

Background: Dental caries is one of the most common infectious diseases and often occurs in the community caused by bacteria. Attached bacteria in the tooth surface for a long time will form a biofilm and will lead to demineralization characterized by damage in the structure of the tooth enamel. The bacteria that cause dental caries and can form biofilms is Streptococcus mutans. The bacteria inside biofilms are more resistant to antibacterial agents. Flavonoids in mangosteen pericarp extract can be a cleaner alternative for the anti-biofilm cavity that has properties against Streptococcus mutans. Purpose: To determine the activity of flavonoids in mangosteen pericarp extract at a certain concentration against Streptococcus mutans bacteria. Methods: This study was a laboratory experimental study with a post-test only control group design. Streptococcus mutans were diluted according to the Mc Farland dilution standard 106 in Tryptic Soy Broth (TSB) medium and put in a flexible U-bottom microtiter plate. Then it was incubated for 5x24 hours and checked using crystal violet simple staining to see the formation of biofilms. Flavonoid extract of mangosteen pericarp performed serial dilution in a concentration of 100%, 50%, 25%, 12.5%, 6.25%, 3.125%, 1.56%, and 0.78% was added, and the incubation process were conducted for 1x24 hours. OD (Optical Density) readings were done with a wavelength of 595 nm. Results: There was a significant difference between the test groups and the positive control group. The concentration of 100% had the anti-biofilm activity and showed the value of the highest percentage of inhibition, whilst the concentration of 0.78% showed a minimum biofilm inhibition concentration. The results were demonstrated by a statistical analysis test. Conclusion: Flavonoid extract of mangosteen pericarp at a certain concentration has anti-biofilm activity against Streptococcus mutans biofilm.
Photodynamic Therapy 405 nm Diode Laser as Antibacterial for Cavity and Root Canal Sterilization Tasya Nafatila Chalisha; Nadira Nurin Febrianti; Sri Kunarti; Setyabudi Setyabudi
Conservative Dentistry Journal Vol. 11 No. 2 (2021): July - December
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/cdj.v11i2.2021.62-66

Abstract

Background: The goals of caries restoration and endodontic treatment are to repair and prevent the infection fromgetting worse and if possible, heal the damaged tissue. To achieve this goal, it is necessary to control the presence ofmicrobes in the cavity or root canals with chemo mechanics prior to filling or obturation of the root canals. Disinfectionmethods using disinfectants with effective bactericidal activity are mostly used at subtoxic levels and at concentrationswhere their toxicity is a significant factor. In addition, the disinfection method is considered unable to achieve thoroughcavity cleaning and causes secondary infection. A new method to provide better disinfection without cytotoxic effectshas recently been discovered using the photodynamic method of 405 nm diode laser therapy. Research continues and isprogressing with the existence of various factors that affect the effectiveness of the 405nm diode laser as an antibacterial.Purpose: To evaluate the results of research on photodynamic diode laser therapy with a wavelength of 405 nm as acombination antibacterial therapy in cavity and root canal sterilization techniques. Review(s): Literature study in the formof narrative review using libraries obtained through the PubMed and Google Scholar databases. The optimal bacterialmortality was influenced by the form factor of the target bacteria, the energy dose and duration of laser exposure, and thetype of photosensitizer used. Conclusion: The use of a 405 nm diode laser with an energy power of 50 mW with a distanceof 20 mm can degrade biofilms Streptococcus mutans up to 100% using erythrosine photosensitizer, for 75 seconds. Andwith the same power and distance, it can degrade the biofilm of bacteria Enterococcus fecalis up to 97.51%, using aphotosensitizer chlorophyll, for 120 seconds.
Management Of Multidiciplinary Aesthetic Treatment In Rotation And Crossbite Tooth With Avulsion Due To Trauma Injuries Nanik Zubaidah; Nadira Nurin Febrianti; Ivan Nur Fadela; Cindy Ramadhan Putri; Muhammad Dimas Aditya Ari Ari; Nirawati Pribadi; Sri Kunarti
Conservative Dentistry Journal Vol. 11 No. 2 (2021): July - December
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/cdj.v11i2.2021.98-102

Abstract

Background: Anterior teeth that experience rotation and crossbite also lose avulsed teeth due to trauma but can be treated with endo restoration treatment. Loss of anterior teeth due to trauma can impact the appearance and decrease self-confidence due to aesthetic factors and phonetic disorders. Objective: to provide information about the management of anterior teeth that undergo rotation and crossbite accompanied by avulsion due to traumatic injury by correcting the position of the teeth through a multidisciplinary approach. Case Report: A 21-year-old woman with 12 teeth had distobuccal rotation and 22 crossbite, while her front teeth 11 and 21 were avulsed due to trauma from falling from a motorcycle. Patients feel embarrassed and lack confidence when laughing and experience phonetic disturbances when speaking. According to the stomatognathic system, the patient comes to the clinic to treat his front teeth to restore their shape, esthetics, and function. Case management: Endo restoration treatment was carried out on 12 rotated teeth and 22 crossbites through root canal treatment, insertion of posts, and cores which will be used as abutments in the manufacture of fixed-fixed bridges avulsed teeth to rehabilitate function, anatomical and aesthetic forms. Conclusion: Teeth that experienced rotation, crossbite, and avulsion due to trauma can be treated with multidisciplinary treatment through endo restoration and fixed-fixed bridge to rehabilitate stomatognathic function.
Degradation of Fusobacterium nucleatum biofilm and quantity of reactive oxygen species due to a combination of photodynamic therapy and 2.5% sodium hypochlorite Nanik Zubaidah; Sukaton; Sri Kunarti; Meidi Kurnia Ariani; Dawailatur Rahman Setiady; Dur Muhammad Lashari
Dental Journal Vol. 56 No. 2 (2023): June 2023
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/j.djmkg.v56.i2.p132-138

Abstract

Background: The persistence of microorganisms in the root canal system is one of the leading causes of root canal treatment failure. Biofilms of putative pathogens hidden inside dentin tubules and other root canal ramifications may limit current disinfection protocols. Photodynamic therapy (PDT) with a wavelength of 628 nm can be used as an antimicrobial strategy that uses low-power laser energy to activate a non-toxic photosensitizer to produce singlet oxygen with the ability to kill microorganisms in root canals. Fusobacterium nucleatum was used because this bacterium is one of the bacteria involved in root canal infection. Purpose: The aim of this study was to compare the bactericidal efficacy of sodium hypochlorite (NaOCl) 2.5%, PDT, and a combination of PDT and NaOCl 2.5% against Fusobacterium nucleatum. Methods: Mature biofilm Fusobacterium nucleatum was divided into four groups according to the protocol of decontamination: K1 (negative control – biofilm), K2 (NaOCl 2.5%), K3 (PDT), and K4 (NaOCl 2.5% + PDT). Biofilm degradation was observed using optical density (OD) at 570 nm using a microplate reader. A reactive oxygen species quantity check was carried out using a nitroblue tetrazolium test, and OD observation was done with a microplate reader at 540 nm. Results: Group 4 (NaOCl 2.5% + PDT) showed more biofilm bacteria elimination than the other groups. Conclusion: A combination of PDT and NaOCl 2.5% can be considered an effective protocol for the elimination of Fusobacterium nucleatum. There is a potentiation relationship between NaOCl 2.5% and PDT FotoSan. Biofilm degradation occurs because of the effect of antibacterial NaOCl 2.5% and the irradiation effect of the Toluidine blue O photosensitizer.
Co-Authors A. Amanda Adioro Soetojo Agus Subiwahjudi Ajeng Hayyuning Citrasari Amellia Tjandra Ananta Tantri Budi Andrie Handy Kusuma Anin Dita Anuj Bhardwaj Ari Subiyanto Aulia Ramadhani Ceples Dian Kartika W.P Cindy Ramadhan Putri Dawailatur Rahman Setiady Debby Fauziah Suryani Dur Muhammad Lashari Dur Muhammad Lashari Edhie Arief P Edhie Arif Prasetyo Elsandra Novita Halim Erin Imaniar B Evri Kusumah Ningtyas Fauziah Diajeng Retnaningsih Ira Widjiastuti Irfan Dwiandhono Ivan Nur Fadela Karlina Samadi Kun Ismiyatin Laksmiari Setyowatie Laskmiari Setyowati Leidy Herlin Rumbiak Meidi Kurnia Ariani Muhammad Dimas Aditya Ari Nadhifa Salma Nadira Nurin Febrianti Nadira Nurin Febrianti Nadya Ayusandra Larasati Nadya Nathania Nanik Zubaidah Nirawati Pribadi Nur Ariska Nugrahani Raditya Nugroho Riski Setyo Avianti Ruslan Effendy Setyabudi Setyabudi Sinta Puspita Sukaton, Sukaton Tasya Nafatila Tasya Nafatila Chalisha Vina Zavira Nizar Widya Saraswati