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All Journal ODONTO Dental Journal Dental Journal (Majalah Kedokteran Gigi) Conservative Dentistry Journal Indonesian Journal of Dental Medicine Indian Journal of Forensic Medicine & Toxicology Odonto dental journal

Ira Widjiastuti

Faculty Of Dental Medicine, Airlangga University

Author-ID : 3197038

Dentistry Health Professions Medicine & Pharmacology Public Health

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PREVALENSI KARIES GIGI POSTERIOR BERDASARKAN KEDALAMAN, USIA DAN JENIS KELAMIN DI RSGM FKG UNAIR TAHUN 2014 (Prevalence of Posterior Teeth Caries by The Depth of Cavity, Age and Gender at RSGM FKG UNAIR in 2014) Amelia Kristanti Rahardjo Rahardjo; Ira Widjiastuti; Edhie Arif Prasetyo
Conservative Dentistry Journal Vol. 6 No. 2 (2016): July - December
Publisher : Universitas Airlangga

Background. Dental caries is one of the most prevalent chronic disease of people worldwide, and also Indonesia. Based on Indonesian Basic Research from Health Department, prevalence of dental caries in Indonesia was 90.5%. When dental caries rates are reported by gender, female are found to exhibit higher prevalence rates than male. Dental caries is disease of lifetime because it can spread out more throughout life and will continue until the teeth are destroyed without a proper treatment. Purpose. The aim of this study is to analyze the prevalence of posterior teeth caries by the depth of cavity, age, and gender at RSGM FKG UNAIR. Method. Analytic observational study with cross-sectional and stratified random sampling method. Result. Out of 420 patients with teeth caries, 169 male patients (40.2 %) and 251 female patients (59.8 %). The highest prevalence is deep caries (59.76%) and the lowest prevalence is superficial caries (15.95%). Conclusion. It was concluded that there is correlation between the depth of cavity increase by age and female has higher caries prevalence than male but no significance differences between cavity depth and gender.

Efektivitas Ekstrak Daun Belimbing Wuluh (Averrhoa bilimbi Linn) sebagai Bahan Pembersih Saluran Akar Gigi (The Effectiveness of Wuluh Starfruit (Averrhoa bilimbi Linn) Leaf Extract as Root Canal Cleaner) Amalia Dara N; Ira Widjiastuti; Laksmiari Setyowati
Conservative Dentistry Journal Vol. 6 No. 2 (2016): July - December
Publisher : Universitas Airlangga

Background: Preparation is one of the stages in endodontic treatment that always produce debris as a result of intrumentation on root canal walls. Debris consist of organic and inorganic materials. Debris will impede the penetration of disinfection materials and reduce attachment of the root canal filling material, therefore needed root canal irrigant which can remove the debris. Wuluh starfruit leaf extract (Averrhoa bilimbi Linn) contain various active compound, such as saponin. Saponin act as “surfactants” which can remove the debris. Purpose: This study was to determine the cleaning effectiveness concentration of wuluh starfruit leaf extract which can clean the root canal walls from debris. Method: Twenty five first premolar human teeth with straight single roots were randomized into five groups in which each group consist of five teeth. Those teeth instrumented with K-file and irrigated as follows: Group 1 (control) used aquadest as irrigant, group 2 used extract 5%, group 3 used extract 10%, group 4 used extract 15%, and group 5 used extract 20%. Then, those teeth were split horizontally and longitudinally in 1/3 apex. After that, the apical third of root canal walls were observed by using scanning electron microscope (SEM). Result: There were significant differences between each group (p<0,05). Group 4 and 5 had smallest median score that was 1. Saponin in wuluh star fruit leaf extract 15% has been reach critical micelle concentration (CMC). Conclusion: Wuluh starfruit leaf extract 15% effective for cleaning root canal walls from debris.

Konsentrasi Efektif Ekstrak Daun Salam (Syzygium polyanthum Wight) terhadap Hambatan Biofilm Enterococcus faecalis (Effective Concentration of Bay Leaf Extract (Syzygium polyanthum Wight) to Inhibit Enterococcus faecalis Biofilm) Shufiyah Nurul Aini; Ruslan Effendy; Ira Widjiastuti
Conservative Dentistry Journal Vol. 6 No. 2 (2016): July - December
Publisher : Universitas Airlangga

Background. Enterococcus faecalis is the most dominant microorganisms found in endodontic secondary infection with prevalence ranging between 24% - 77%. Defense mechanism of Enterococcus faecalis bacteria is forming biofilm,. A study showed that bacteria in mature biofilms can 10-1000 times more resistant to antimicrobials than bacteria in a planktonic form. One of the natural substances that can be used as antibiofilm to irrigation root canals is extract of fresh bay leaf (Syzygium polyanthum Wight). Chemical components in bay leaves include flavonoids, tannins, and essential oils, which have antibacterial capability and damage the membrane biofilm. Purpose. To determine the effective concentration of fresh bay leaf extract (Syzygium polyanthum Wight) that can inhibit biofilm Enterococcus faecalis. Method. This research is in-vitro labolatory experimental with post test only control group design using microtitter plate assay. Samples using Enterococcus faecalis ATCC 29212 cultured in TSB (Trypticase Soy Broth) + glucose.Bay leaf extract (Syzygium polyanthum Wight) concentration in this study was 13%, 12.25%, 11.50%, 10.75%, 10%, 9.25%, 8.50%, 7.75%, 7%, and 6.25%. Results. At the 13% concentration of Syzygium polyanthum Wight, showed 100% inhibition of biofilm, means that the 13% concentration of bay leaf extract (Syzygium polyanthum Wight) can totally inhibit biofilm formation of Enterococcus faecalis. Conclusion. The effective concentration of bay leaf extract (Syzygium polyanthum Wight) which inhibit Enterococcus faecalis biofilm is 13%.

Konsentrasi Bunuh Minimal (KBM) Ekstrak Propolis Lawang terhadap Candida albicans Alvita Wibowo; Ira Widjiastuti; Widya Saraswati
Conservative Dentistry Journal Vol. 7 No. 1 (2017): January - June
Publisher : Universitas Airlangga

Background: Candida albicans is the most resistant fungi in root canals. The existence of Candida albicans after root canal treatment can cause the failure of root canal treatment. The prevalence of this case is 36,7%. Propolis is a resinous substance which has many pharmaceutical and biological effects such as antimicrobial activities. Its active components include flavonoid, saponin, and tannin, have an important role in antimicrobial activities. In this study, antifungal aspect of propolis extract was investigated. Purpose: The aim of this study was to determine the minimum fungicidal concentration (MFC) of propolis extract againts Candida albicans. Methods: This research was a laboratory experimental study. Propolis extract produced by propolis farm from Lawang. The antifungal activity test was performed by colony counting fungi in sabouraud’s dextrose agar (SDA) to determine the value of MFC of propolis extract againts Candida albicans. Result: At the concentration of 72,5%, 73,5%, 74,5%, 75,5%, 76,5%, and 77,5% there are decreases in the number of Candida albicans fungal colonies when compared with positive control group. There are significant differences in each study group (p<0,05). At the concentraion of 76,5% was not revealed any fungal growth of Candida albicans, it was because the antifungal effect of propolis extract has reached MFC. Conclusion: The minimum fungicidal concentration of propolis extract againts Candida albicans is 76,5%.

Antibacterial power effectiveness of calcium hydroxide and propolis mixture on Fusobacterium nucleatum bacteria ira Widjiastuti; S. Sukaton; Agnes Melinda Wong; Nanik Zubaidah
Conservative Dentistry Journal Vol. 9 No. 1 (2019): January - June
Publisher : Universitas Airlangga

Background: Calcium hydroxide is a root canal dressing material that is widely used in dentistry because of its ability to regenerate hard tissue and eliminate bacteria. According to Ferreira et al. (2015), Fusobacterium nucleatum was found in 90% of teeth that had a root canal treatment done using calcium hydroxide as an intracanal medication. Due to this shortcoming of the antibacterial power of calcium hydroxide, additional research on alternative ingredients that can be combined with calcium hydroxide to improve its antibacterial power is necessary. Propolis is a natural material that has high antibacterial power and has long been used in dentistry. The addition of propolis to calcium hydroxide is expected to improve the antibacterial power of calcium hydroxide without eliminating its function in terms of regenerating hard tissue. Purpose: To find out how effective is the antibacterial power of a combination of calcium hydroxide and propolis against Fusobacterium nucleatum. Methods: The research was carried out using 4 treatment groups consisting of 6 samples for each group. Group 1 is given a combination of calcium hydroxide and propolis with a ratio of 1:1, group 2 with a ratio of 1:1.5, group 3 with a ratio of 1:2, and group 4 is a positive control of calcium hydroxide and sterile aquadest suspension. Each sample was put into a test tube containing BHIB and a suspension of Fusobacterium nucleatum, incubated at 37ºC for 24 hours, and vortexed for 1 minute. A total of 0.1 ml of bacterial inoculum was taken from each sample and then put into the MHA and grown for 24 hours. The number of Fusobacterium nucleatum colonies grown on MHA was calculated and expressed using the Colony Forming Unit (CFU). Results: There were fewer colonies of Fusobacterium nucleatum in the treatment group compared to the control group. Conclusion: The combination of calcium hydroxide and propolis has an effective antibacterial power against Fusobacterium nucleatum which the ratio of 1:2 is more effective than ratio of 1:1,5 and 1:1.

Compressive Strength Test on Calcium Hydroxide with Propolis Combination Ira Widjiastuti; S. Setyabudi; M. Mudjiono; Erika Setyowati
Conservative Dentistry Journal Vol. 9 No. 1 (2019): January - June
Publisher : Universitas Airlangga

Background: Calcium hydroxide is a dental material used as a gold standard for pulp capping materials. However, calcium hydroxide has several weaknesses which cause many researchers to look for alternative ingredients that come from nature. Propolis in the field of dentistry has long been used because of its ability as an anti-inflammatory, anti-microbial, anti-fungal, and can cure scars. The combination of calcium hydroxide and propolis is proven to have good biocompatibility and anti-bacterial properties. One of the requirements of pulp capping material is to have sufficient compressive strength. Therefore, a research to test the compressive strength value of the combination of calcium hydroxide with propolis is needed. Purpose: To find out the difference in compressive strength of the combination of calcium hydroxide-propolis with a ratio of 1: 1, 1: 1.5, and 1: 2. Methods: The study used 4 treatment groups with each group consisting of 6 replications. Group 1 is a combination of calcium hydroxide-propolis with a ratio of 1: 1, group 2 with a ratio of 1: 1.5, group 3 with a ratio of 1: 2, and a positive control group using calcium hydroxide - sterile aquadest. Calcium hydroxide powder and propolis extract liquid is mixed according to comparison and printed on a cylindrical mold with the size of 4 mm x 6 mm. Then, the compressive strength was tested using an Autograph test instrument. Result: Compressive strength was smaller in group 3 compared to group 2, group 2 compared to group 1, and group 1 compared to the control group. Conclusion: In calcium hydroxide-propolis combination, the more propolis extract used in the combination the lower the compressive strength of the combination will be.

Effect of Hydrogel Epigallocatechin-3-Gallate (EGCG) to the Number of Fibroblast Cell Proliferation in the Perforation of Wistar Rat Tooth Pulp Ira Widjiastuti; Setyabudi setyabudi; Kun Ismiyatin; Djoko Agus Purwanto; Tiara Sukmawati
Conservative Dentistry Journal Vol. 9 No. 2 (2019): July - December
Publisher : Universitas Airlangga

Background: Pulpitis can occur because the deep cavity preparation and it causes increasing of NO levels. Perforated teeth require direct pulp capping (DPC) treatment. The current standard DPC material is calcium hydroxide. However, several studies have found weaknesses of calcium hydroxide that can affect the success of DPC treatment and new, more biocompatible materials are needed. Epigallocatechin-3-gallate (EGCG) in green tea has many benefits, including antioxidant, anticolagenase, anticancer, anti-inflammatory and has the ability of radical scavenging to clean NO so that pulp healing can occur better by increasing the number of fibroblast cells that play a role in wound healing. Purpose: To determine the concentration of hydrogel EGCGs that are effective in increasing the number of fibroblast cell proliferation in the dental pulp perforation of Wistar rats. Method: This research is a laboratory experimental study with a randomized post test only control group design. Samples used in the study were 24 male Wistar rats which were divided into four groups, namely the negative control group and the treatment group were given EGCG 60 ppm, 90 ppm, and 120 ppm and were decapitated on the 7th day after treatment. The maxilla and the 1st molar were taken and decalcified, to process the HPA reading with HE staining. Observations were made using a microscope with a magnification of 400x. Results: There were significant differences in the treatment groups with 60 ppm and 90 ppm hydrogel hydrogels on the results of the Oneway ANOVA difference test (p <0.05). Conclusion: The concentration of hydrogel EGCG which is effective in increasing fibroblast cell proliferation is 90 ppm.

EFFECT OF CALCIUM HYDROXIDE-PROPOLIS COMBINATION ON THE NUMBER OF FIBROBLAS CELLS AND ANGIOGENESIS IN WISTAR RATS PULP Nirawati Pribadi; Ira Widjiastuti; Agata Nadia
Conservative Dentistry Journal Vol. 10 No. 1 (2020): January-June
Publisher : Universitas Airlangga

Background: The vitality of the pulp must be protected because the pulp contains fibers, cells, and various structures. Pulp vitality can be maintained by pulp capping to stimulate the formation of reparative dentin. The material commonly used for pulp capping is calcium hydroxide. Calcium hydroxide has the disadvantage of causing inflammation and necrosis of the pulp surface. To compensate that, propolis is added because it has the ability as an anti-inflammatory. Purpose: To analyze the increase in the number of fibroblast cells and angiogenesis in the pulp after the application of combination of Calcium hydroxide-propolis compared to the application of Calcium hydroxide.. Methods: This study used 30 samples of wistar rats which were preparated until perforation and then divided into 3 groups. The first group continued with filling with cention, the second group continued with application of calcium hydroxide and filled with cention, and the third group continued with the application of combination of calcium hydroxide and propolis and then being filledwith cention. fibroblast cells and angiogenesis is calculated histopathologically by using compound light microscopeon the 7th day with 400x magnification.Results:Based on the results of the study found a significant difference between the calcium hydroxide group and the calcium hydroxide-propolis group. Increased numbers of fibroblasts and angiogenesis indicate a faster inflammatory process that affects the acceleration of wound healingConclusion: More number of fibroblasts and angiognesis cells found in the pulp after the application of a combination of Calcium hydroxide-propolis compared to the application of Calcium hydroxide

Biocompatibility of 0,78% tannin of garciniamangostanalinnpericarp extract and 0,2% chlorhexidinegluconateagainst BHK-21 fibroblast cells culture Monika Werdiningsih; Ira Widjiastuti; Febriastuti Cahyani; Dian Agustin Wahjuningrum
Conservative Dentistry Journal Vol. 10 No. 1 (2020): January-June
Publisher : Universitas Airlangga

Background.Chlorhexidinegluconate is one of endodontic irrigants potential due to its antibacterial activity. Although it is an effective antibacterial agent, chlorhexidinegluconate cannotdissolve organic substances and necrotic tissue present in the root canal. In addition, same as other chemicals, chlorhexidinegluconate also cause a cytotoxic effect. Tannin extracts of mangosteenpericarp (Garciniamangostana Linn.) demonstrated various biological activities including antibacterial, antioxidant, anticancer and anti-inflammatory. Tannin extracts of mangosteenpericarp can be considered as an alternative endodontic irrigation for dental application.Purpose.The aim of this study was to compare the biocompatibility between 0,78% tannin extracts of mangosteen pericarp and 0,2% chlorhexidinegluconate to BHK-21 fibroblass cell. Method.Tannins obtained from extracts of mangosteenpericarp. Preliminary test was conducted in the biocompatibility of tannin extracts of mangosteenpericarp in various concentrations of the BHK-21 fibroblass. Tannins extracts of mangosteenpericarp was at concentrations of 0,78% less toxic than the other concentrations. Then biocompatibility of 0,78% tannin extracts of mangosteenpericarp compare with 0.2% chlorhexidinegluconate using cytotoxicity test on BHK-21 fibroblass cells with MTT method and observed by ELISA reader. Results.0,78% tannins extracts of mangosteenpericarp showed good biocompatibility with fibroblass BHK-21 than 0,2% chlorhexidinegluconate. There was a significant differences between the results of treatment with 0,78% tannin extracts of mangosteenpericarp and 0.2% chlorhexidinegluconate. Conclusion. 0,78% tannin extracts of mangosteenpericarp has better biocompatibility than 0,2% chlorhexidinegluconate as an endodontic irrigants.

THE EFFECT OF CALCIUM HYDROXIDE-PROPOLIS ON THE NUMBER OF MACROFAG CELLS ON THE WISTAR RAT DENTAL WITH PULPA PERFORMANCE Setyabudi Setyabudi; Devi Eka Juniarti; Ira Widjiastuti; Maughfirah Shintya Fathori
Conservative Dentistry Journal Vol. 10 No. 2 (2020): July-December
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/cdj.v10i2.2020.58-61

Background: Cellular response of dental pulp to existing lesions, caused by infiltration of inflammatory cells that migrate from blood vessels to the site of injury. Macrophages are the cells that most appear when inflammation occurs. Treatment for maintaining pulp tissue vitality is called pulp capping. Calcium hydroxide as a pulp capping material has the ability to trigger the growth of dentin bridges or remineralization, but calcium hydroxide can induce migration and proliferation of inflammatory cells. Currently the field of dentistry is developing propolis as an alternative pulp capping material. That is because propolis has anti-inflammatory properties. Objective: To analyze the effect of the combination of calcium hydroxide-propolis on the number of macrophage cells in the teeth of wistar rats with pulp perforation. Method: This study used 30 samples of Wistar rats which were preparated until perforation and then divided into 3 groups. The first group continued with filling with cention, the second group continued with application of calcium hydroxide and filled with cention, and the third group continued with the application of combination of calcium hydroxide and propolis and then being filledwithcention. Macrophage cell is calculated histopathologically by using compound light microscope on the 3rd day with 400x magnification. Results: The Kruskal-Wallis test results showed significant differences in macrophages after the combination of calcium hydroxide and propolis (p = 0,000). The combined application of calcium hydroxide and propolis showed higher macrophages than the application of calcium hydroxide and the control group. Conclusion: Application of calcium hydroxide - propolis combination was proven increase the number of macrophage cells in Wistar Rats (Rattus Norvegicus) with pulp perforation

Co-Authors Adeline Jovita Tambayong Adioro Soetojo Aga Satria Nurrachman Agata Nadia Agnes Melinda Wong Agus Subiwahjudi Agus Subiwahjudi Alhidayati Asymal Alvita Wibowo Amalia Dara N Amelia Kristanti Rahardjo Rahardjo Andrie Handy Kusuma Annisa Rahma Ari Subiyanto Aulia, Zahra Maulida Cecilia G. J. Lunardhi Chatarina Umbul Wahyuni Chonny Salsabilla Zamrutizahra Christian, Bernardus Daniel Cinitra Anindya Daniyal Lazuardi Ramadhan Debby Fauziah Suryani Derice Putri Nourah Serena Devi Eka Juniarti Devi Puspitasari Dian Agustin Wahjuningrum Djoko Agus Purwanto Dwina Rahmawati Junaedi Edhie Arif Prasetyo Eric Priyo Prasetyo Erika Setyowati Evri Kusumah Ningtyas Fauziah Diajeng Retnaningsih Febriastuti Cahyani Febriastuti Cahyani Galih Sampoerno Grace Angelina Samuel Hanun, Hafshah Zhafirah Siri Irwantoro, Edward Ivan Nur Fadela Ketut Suardita, Ketut Kharisna, Deaniddo Kun Ismiyatin Kuntaman Kuntaman Laksmiari Setyowati M. Aditya Syahputra M. Mudjiono Mandojo Rukmo Maughfirah Shintya Fathori Michael Golden Kurniawan Moch. Mudjiono Mochammad Raidjffan Zulkarnaen Tabona Monika Werdiningsih Nabila, Rizka Affan Nadia Irnatari Nancy Cynthia Sudiartha Nanik Zubaidah Nastiti Faradilla Ramadhani Ni Putu Sawitri Mega Nia Nur Haliza Nirawati Pribadi Nur Dianawati Nurkhalidah, Binar Najwa Pramesty, Azzahra Kinaya Putra, Mohammad Gerald Athallah Rafania Atikah, Ayu Raftiani, Ardelia Sabrina Rendy Popyandra Rini Devijanti Ridwan Riyan Iman Marsetyo Ruslan Effendy Ruslan Effendy Sagita Putri Andyningtyas Salsabilla Zamrutizahra, Chonny Serena, Derice Putri Nourah Setyabudi Setyabudi Setyabudi Shufiyah Nurul Aini Sri Kunarti Sri Kunarti, Sri Sri Wigati Mardi Mulyani Sukaton, Sukaton Syahria, Hania Dana Tiara Dyah Iswari Tiara Sukmawati Valensia, Theodora Wahyu Setyarini Widya Saraswati Wijanarko, Christina Immee Witjaksana, Annisa Salsabila Yunita Savitri

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