Nirawati Pribadi
Faculty Of Dental Medicine, Universitas Airlangga, Surabaya

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Improving teeth appearance from the impact of unhealthy lifestyle: a case report Pribadi, Nirawati; Linawati; Rachmawati, Atika Nisaa; Ramadhan, Aqila Shabrina Dwi; Fadhillah, Revita Rizki
Conservative Dentistry Journal Vol. 14 No. 2 (2024): July-December
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/cdj.v14i2.2024.75-78

Abstract

Background: Tooth discoloration in one or more anterior teeth can cause significant aesthetic problem for patients because it affects the smile that relates to a person’s self-confidence. Thus, external bleaching is a viable treatment alternative. Discoloration stains on teeth are caused by smoking and the consumption of pigmented beverage such as coffee. Purpose: This case reports an external bleaching caused by an unhealthy lifestyle. Case: A 26- year-old female television presenter wanted to treat yellowish dark shade of her teeth and this made her feel less confident due to the yellowish dark shade teeth. Case Management: Teeth discoloration was managed with external bleaching using hydrogen peroxide 40%. After the treatment, the teeth shade increased from yellow-brownish to A2 with Vita Classic shade guide. Conclusion: The case report shows that external bleaching treatment produces brighter shade and increase self-confidence of the patient so she feels very satisfied.
Antibiofilm activity of probiotic bacteria Lactobacillus plantarum FNCC 0020 against Streptococcus mutans serotype c Pribadi, Nirawati; Sampoerno, Galih; Juniarti, Devi Eka; Goenharto, Setyabudi; Zubaidah, Nanik; Ramadhini Arrianti, Tyas; Ramadhani, Aqila Shabrina Dwi; Fadhillah, Revita Rizki
Conservative Dentistry Journal Vol. 15 No. 1 (2025): January-June
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/cdj.v15i1.2025.33-37

Abstract

Background: Streptococcus mutans (S. mutans) is a Gram-positive bacterium that plays a role in dental caries. Plaque and biofilm formation can be chemically cleaned using mouthwash, such as chlorhexidine gluconates (CHX) 0.2%, which is the gold standard. Another alternative can be developed to inhibit S. mutans biofilm formation by using Lactobacillus plantarum probiotic, which has antibiofilm potency by producing antimicrobial substances. Purpose: This study tested the biofilm inhibition to determine the Inhibitory Concentration that can inhibit 50% of biofilm formation. Methods: This research was conducted as an in vitro experimental laboratory study. Biofilm inhibition testing was performed by using the microdilution method on biofilms formed in 96 well microplates with 0,1% crystal violet staining. Lactobacillus plantarum FNCC 0020 was diluted to several concentrations: 12.5%, 25%, and 50% against Streptococcus mutans biofilm induced by 3% sucrose. The test results in optical density were read using a spectrophotometry with a wavelength of 650 nm. were determined using the GraphPad Prism sigmoidal dose-response method. Results: There is antibiofilm activity of Lactobacillus plantarum FNCC 0020 against Streptococcus mutans, and the inhibitory effect against Streptococcus mutans biofilm showed of 42.43%. Conclusion: Lactobacillus plantarum FNCC 0020 at 50% has biofilm inhibitory activity compared to other concentrations but is less effective than 0.2% CHX.
Epigallocatechin-3-gallate (EGCG) and tricalcium silicate (C_3 S) combination as an antibacterial agent against Enterococcus faecalis Ismiyatin, Kun; Cahyani, Febriastuti; Soetojo, Adioro; Widjiastuti, Ira; Pribadi, Nirawati; Nurkhalidah, Binar Najwa; Raftiani, Ardelia Sabrina; Pramesty, Azzahra Kinaya; Anindya, Cinitra
Conservative Dentistry Journal Vol. 15 No. 1 (2025): January-June
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/cdj.v15i1.2025.46-48

Abstract

Background: Dental caries affects 57.6% of the Indonesian population, with Enterococcus faecalis found in 80% of deep caries cases. Conventional materials used in vital pulp therapy (VPT), such as calcium hydroxide, have limitations including transient antibacterial action and poor sealing capacity. This prompts the exploration of alternative materials like epigallocatechin-3-gallate (EGCG), a polyphenol from green tea with antimicrobial activity, and tricalcium silicate (C₃S), a bioactive cement known for dentin regeneration potential. Purpose: This review evaluates the synergistic antibacterial potential of EGCG and C₃S combinations against E. faecalis, and assesses their feasibility as an alternative in VPT. Review: EGCG disrupts bacterial membranes, inhibits biofilm formation, and suppresses virulence factors, but lacks remineralization capability. Conversely, C₃S promotes dentin repair and creates an alkaline antibacterial environment, though its efficacy diminishes over time. Their combination compensates for each other’s limitations: EGCG enhances antimicrobial potency while C₃S provides long-term alkalinity and regenerative support. Conclusion: The EGCG–C₃S combination exhibits synergistic antibacterial and regenerative effects, offering a promising alternative to current VPT materials.