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Evaluation of Biological Degraded Keratin for Biogas Production Using Dry Anaerobic Digestion System Sinta Setyaningrum; Regina J Patinvoh; Ronny Purwadi; Mohammad Taherzadeh
Jurnal Internasional Penelitian Teknologi Terapan Vol 2 No 2 (2021): October 2021
Publisher : Bandung State Polytechnic (Politeknik Negeri Bandung)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35313/ijatr.v2i2.49

Anaerobic digestion is a methane gas production process that can be used as sustainable alternative energy. Anaerobic digestion utilized various types of organic waste as substrate for the reaction process. Keratin waste is an organic waste mainly produced from the poultry and farming industry. Pretreatment is usually required to hydrolyzed keratin protein complex as the amino acid is easily used as the substrate in anaerobic digestion reaction. Biological pretreatment was selected because it more energy saver and generating diverse types of amino acid monomers. Three types of keratins used in this research were feathers, wool, and hair. Culture of Bacillus sp. C4 were inoculated into keratins and incubated for 24 hours, 48 hours, and 72 hours. The chicken feathers produce the soluble protein as much as 7.23 mg/ml, 32.59 mg/ml and 45.99 mg/ml respectively, while the sheep wool produce 24.08 mg/ml, 36.73 mg/ml and 38.75 mg/ml respectively according to incubation time. Meanwhile, keratin hair cannot be degraded by Bacillus sp. C4 at all. Free ammonia formed by hydrolysis of proteins is suspected to be an inhibitor in the methanogenesis process, as total methane produced from degraded keratin only 256,6 ml C4/gr VS in 36 days retention time.

Adsorpsi Logam Cu dengan Graphene Oksida Raihan Nurfauziah; Zulfa Rahmah Fadillah; Sinta Setyaningrum; Eko Andrijanto
Jurnal Kimia Fullerene Vol 6 No 2 (2021): Fullerene Journal of Chemistry
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37033/fjc.v6i2.326

The development of industry around the river causes an increase in pollutants flowing in the river. One type of industrial pollutant is heavy metals. Heavy metals are toxic and toxic, so the presence of heavy metals in the aquatic environment can damage aquatic ecosystems. One of the ways to remove heavy metals is by using the isotherm adsorption method. Namely the process where the absorption of heavy metals on the surface of solids, namely the adsorbent. The adsorbent used is a graphene oxide sand composite which is made from carbon and has a high potential to remove heavy metal pollutants. The experiment was carried out by mixing the adsorbent with a solution of Cu2+ metal ions and adsorption was carried out using several parameters, namely variations in contact time and adsorption pH and measurements were made using a visible spectrophotometer. Optimum absorption occurs at the 25th minute contact time with an efficiency of 96.2% and at an adsorption pH of 8 with an efficiency of 99.4%. So it can be concluded that graphene oxide sand composite can be used to remove heavy metal pollutants.

Perancangan Primer untuk Deteksi Kandungan Gen Cytochrome b Babi dengan Metode Polymerase Chain Reaction dan Aplikasinya pada Berbagai Produk Industri Dianti Devi Astari; Sania Gustiani Dewi; Sinta Setyaningrum; Bevi Lidya
Jurnal Kimia Fullerene Vol 6 No 2 (2021): Fullerene Journal of Chemistry
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37033/fjc.v6i2.329

Types of meat from food products that circulating in the market can be detected by Polymerase Chain Reaction with the cytochrome b gene as a marker. The most important and characteristic component for the PCR reaction is the primer, which is an oligonucleotide that acts as an initiator in the PCR reaction. In this study, the primer designed was intended to amplify the pig cytochrome b gene. The design was carried out with the help of the Primer-BLAST application, and manually, three pairs of primers came from BLAST primers, and one pair of primers was designed manually. The primer that has been designed is tested on pork, beef, and food industry products, such as pork sausage, beef sausage, and chicken sausage. The results of PCR amplification were identified qualitatively using 1% Agarose Gel Electrophoresis and visualized using a UV transilluminator. The primer designed manually to produce the whole pig cytochrome b gene with a product size of 1140 bp has succeeded in amplifying the product and is specific because it only produces bands in pork samples, while in chicken and beef samples, it does not produce bands. The three pairs of primers suggested by Primer-BLAST could amplify the porcine cytochrome b gene with band sizes of 121 bp, 272 bp, and 262 bp, but they are not specified yet because it also can amplify the gene in beef and chicken samples. The manual primer design is the best primer and can be further used to detect foodstuffs contains pig genes by PCR.

Coaxyl-mask: Masker Ramah Lingkungan dari Sabut Kelapa (Cocos nucifera) dan Acetobacter xylinum Sinta Setyaningrum; Zahra Nur Salsabila; Aisyah Auliya Rahmawati; Anindya Indrita Putri; Dhea Nurul Amalia; Syakira Athiya Tsany
Fluida Vol 15 No 1 (2022): FLUIDA
Publisher : Jurusan Teknik Kimia, Politeknik Negeri Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35313/fluida.v15i1.3481

Penggunaan masker bagi setiap orang dinilai efektif untuk membatasi penyebaran Covid-19. Namun, permasalahan lingkungan muncul akibat dari banyaknya penggunaan masker karena masker sekali pakai sulit untuk terurai secara alami. Maka dari itu, Coaxyl-mask merupakan inovasi masker sekali pakai yang terbuat dari bioselulosa hasil fermentasi Acetobacter xylinum dan sabut Cocos nucifera. Masker ini terdiri dari 3 lapisan yaitu film bioselulosa yang memiliki sifat water repellent, filter sabut kelapa, dan film bioselulosa sehingga mampu terdekomposisi dengan cepat jika dibandingkan dengan masker sekali pakai pada umumnya. Pada gagasan yang diusulkan ini, perancangan teknik implementasi Coaxyl-mask yaitu dengan melibatkan banyak pihak seperti, pedagang kelapa, produsen masker, tim riset perusahaan medis, dan pabrik independen. Diprediksi masker ini akan memiliki manfaat yang panjang sebagai alat pelindung pada saat pandemi dan mampu menjadi solusi dalam menangani pencemaran atau penimbunan masker seiring dengan jumlah pemakai yang banyak. Wearing masks has been proven to be the most effective in preventing and minimising the spread of Covid-19. However, there is an environmental risk along with the growing demand of single-use masks. This is caused as the single use mask waste cannot be degraded naturally. Therefore, Coaxyl-mask is a green innovation of masks that is made from biocellulose produced by fermentation of glucose using Acetobacter xylinum and coconut husk waste. This mask is made of three layers of water repellent biocellulose film, a filter made from coconut husk, and another biocellulose film that is non-irritative to the skin. As the materials were made of natural materials, this mask can degrade naturally faster compared to conventional single use masks, so it is environmentally friendly. To implement this idea, there need to be a lot of party involved such as coconut seller, mask producers, medical equipment research team, and independent factories. Because its biodegradable materials, this mask will be a revolutionary invention in medical industry and will be very useful in preventing diseases while saving the environment.

BACTERIAL CORE FLOODING ON LIMESTONE ARTIFICIAL CORE FOR MICROBIAL ENHANCED OIL RECOVERY (MEOR) APPLICATION Sinta Setyaningrum
PETRO:Jurnal Ilmiah Teknik Perminyakan Vol. 10 No. 4 (2021): DESEMBER
Publisher : Jurusan Teknik Perminyakan Fakultas Teknologi Kebumian dan Energi Universitas Trisakti

Microbial Enhanced Oil Recovery (MEOR) is a tertiary methods to increase oil production using bioproduct of microorganisms form acidic compounds, gases, polymers, and surfactants, as an agent that can alter the characteristics of the reservoir rock and oil. Previous laboratory research indicated that bacterial isolates indigen from petroleum reservoir, MSD4 and MSD5, with the amount of inoculum ratio 1:1 in recovery medium can reduce the viscosity value and interfacial tension of oil. The aim of this research was to determine the amount of oil recovery from low saturated oil in limestone artificial core by using bacterial core flooding method, also to determine the physical character and chemical character changes in limestone rocks and petroleum. Each bacteria, MSD4 and MSD5, were adapted to the medium SMSS that have been added by 5% oil. Inoculum of indigen bacterial cultures to be injected into the limestone core was bred in the SMSS culture medium added molasses with a varied amount, as much as 1%, 2%, and 3%. From the those variation, the amount of molasses added as much as 1% in the medium SMSS, is known that MSD4 and MSD5 cultures can grow to the highest μ respectively at the age of 16-hour and 24-hour with a value at 0.9992 hours-1 and 0, 9993 hours-1. Inoculum MSD4 and MSD5 cultures with the amount of 1:1 ratio and each bacterial cell density 107-108 cells/mL, were injected into artificial limestone cores with oil saturated less than 15%. The value of rock porosity range used for the simulation of bacterial flooding is 37% -42%, while permeability range used low permeability value (1mD-10mD) and fair permeability value (10mD-50mD). High oil recovery factor (RF%) were obtained from bacterial flooding simulation and incubated for 7 days in a temperature of 55°C on medium SMSS for cores with low permeability values and fair, has a range of 16,3% - 32% and 12% -15,3%. The result of microorganisms activity in limestone rocks from a change on physical character, have a range at -41,8% - 698% on permeability value and a range at -63,85% - 1,03% on porosity value. Indicator of bacterial attachment on limestone cores can be determined by photomicrographs analyzing Scanning Electron Microscope and Energy Dispersive X-ray Spectroscopy.

Pemanfaatan Ekstrak Daun Jambu Biji Sebagai Inhibitur Korosi Baja Paduan dalam Medium Larutan NaCl Agustinus Ngatin; Annisaa Fitri Wulandari; Asri Dwi Saffanah; Dian Ratna Suminar; Sinta Setyaningrum
Fluida Vol 15 No 2 (2022): FLUIDA
Publisher : Department of Chemical Engineering, Politeknik Negeri Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35313/fluida.v15i2.3923

Korosi merupakan masalah yang tak dapat dihindari oleh industri. Salah satu upaya untuk menekan laju korosi adalah dengan menggunakan inhibitor korosi. Daun jambu biji (Psidium guajava L.) mengandung antioksidan berupa tanin yang dapat dimanfaatkan sebagai inhibitor korosi organik ramah lingkungan pada baja paduan tinggi dalam medium larutan NaCl 3,56% (b/v). Ekstrak daun jambu biji didapatkan dengan metode maserasi, sedangkan pengkorosian dengan metode perendaman. Penelitian ini bertujuan untuk mengkaji pengaruh konsentrasi inhibitor ekstrak daun jambu biji dan waktu pengkorosian terhadap laju korosi dengan efisiensi inhibisinya pada baja paduan tinggi dalam medium larutan NaCl 3,56%. Hasil penelitian menujukkan bahwa laju korosi terendah dicapai pada konsentrasi inhibitor ekstrak 400 ppm sebesar 0,0244 mm/y dengan efisiensi inhibisi 53,03%. Pengaruh waktu pengkorosian baja paduan tinggi pada konsentrasi inhibitor ekstrak 400 dan 800 ppm menghasilkan laju korosi terendah pada waktu 192 jam, yaitu 0,0301 dan 0,0282 mm/y. Penggunaan ekstrak daun jambu biji sebagai inhibitor korosi berhasil menurunkan laju korosi baja paduan tinggi dalam medium larutan NaCl 3,56%.

Peningkatan Mutu Produk Serbuk Herbal Melalui Ekstraksi dan Kristalisasi Simplisia oleh KWT Rengganis Desa Arjasari Riniati Riniati; Nancy Siti Djenar; Lili Indrawati; Sudrajat Harris Abdulloh; Sinta Setyaningrum; Nurcahyo Nurcahyo
Wikrama Parahita : Jurnal Pengabdian Masyarakat Vol. 7 No. 1 (2023): Mei 2023
Publisher : Universitas Serang Raya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30656/jpmwp.v7i1.5371

Kelompok Wanita Tani (KWT) Rengganis di Desa Arjasari Kabupaten Bandung merupakan kelompok yang bergerak dibidang pengolahan hasil panen dari petani setempat menjadi produk yang bernilai jual lebih ekonomis. Salah satu kegiatan yang telah dilakukannya adalah mengolah tanaman seperti jahe, kunyit, dan rempah lainnya menjadi serbuk dan diolah menjadi minuman herbal. Kegiatan tersebut sangat bermanfaat mengingat pada saat ini minuman herbal sebagai bahan aditif yang dapat meningkatkan daya tahan tubuh semakin diminati oleh masyarakat luas. Tujuan PkM ini adalah meningkatkan masa simpan bahan baku herbal dengan cara mengubahnya menjadi simplisia, selanjutnya dijadikan serbuk dan diekstraksi untuk dijadikan serbuk melalui proses kristalisasi pada suhu 90°C selama kurang lebih 1 jam. Program PkM Polban ini diawali dengan memberikan beberapa materi tentang peningkatan mutu produk yang dimulai dari persiapan bahan, pencucian, pengeringan, ekstraksi dan kristalisasi. Selanjutnya mempraktikkan cara menggunakan alat perajang, grinding dan kristalisasi. Untuk meningkatkan baik jumlah dan mutu dari serbuk herbal siap minum ini juga diperkenalkan tentang metode lain yaitu maserasi bertahap. Pasca penyuluhan, metode ini telah diterapkan oleh KWT dalam produksi jahe merah dan terbukti dapat meningkatkan rasa dan jumlah produknya. Seiring dengan itu permintaan pasar saat ini sudah mulai meningkat dengan indikasi naiknya jumlah penjualan per bulannya hingga hampir 200%. Target ke depan diharapkan KWT Rengganis dapat menampung hasil tanaman herbal dari petani setempat lebih banyak lagi dengan mengeringkan menjadi simplisia terlebih dahulu sebelum diolah menjadi produk. Dengan demikian produksi dapat menjadi lebih efisien dan ekonomis. Selain itu diharapkan kedepan KWT Rengganis memiliki rumah produksi yang memenuhi standar SNI.

Coaxyl-mask: Masker Ramah Lingkungan dari Sabut Kelapa (Cocos nucifera) dan Acetobacter xylinum Sinta Setyaningrum; Zahra Nur Salsabila; Aisyah Auliya Rahmawati; Anindya Indrita Putri; Dhea Nurul Amalia; Syakira Athiya Tsany
Fluida Vol 15 No 1 (2022): FLUIDA
Publisher : Department of Chemical Engineering, Politeknik Negeri Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35313/fluida.v15i1.3481

Penggunaan masker bagi setiap orang dinilai efektif untuk membatasi penyebaran Covid-19. Namun, permasalahan lingkungan muncul akibat dari banyaknya penggunaan masker karena masker sekali pakai sulit untuk terurai secara alami. Maka dari itu, Coaxyl-mask merupakan inovasi masker sekali pakai yang terbuat dari bioselulosa hasil fermentasi Acetobacter xylinum dan sabut Cocos nucifera. Masker ini terdiri dari 3 lapisan yaitu film bioselulosa yang memiliki sifat water repellent, filter sabut kelapa, dan film bioselulosa sehingga mampu terdekomposisi dengan cepat jika dibandingkan dengan masker sekali pakai pada umumnya. Pada gagasan yang diusulkan ini, perancangan teknik implementasi Coaxyl-mask yaitu dengan melibatkan banyak pihak seperti, pedagang kelapa, produsen masker, tim riset perusahaan medis, dan pabrik independen. Diprediksi masker ini akan memiliki manfaat yang panjang sebagai alat pelindung pada saat pandemi dan mampu menjadi solusi dalam menangani pencemaran atau penimbunan masker seiring dengan jumlah pemakai yang banyak. Wearing masks has been proven to be the most effective in preventing and minimising the spread of Covid-19. However, there is an environmental risk along with the growing demand of single-use masks. This is caused as the single use mask waste cannot be degraded naturally. Therefore, Coaxyl-mask is a green innovation of masks that is made from biocellulose produced by fermentation of glucose using Acetobacter xylinum and coconut husk waste. This mask is made of three layers of water repellent biocellulose film, a filter made from coconut husk, and another biocellulose film that is non-irritative to the skin. As the materials were made of natural materials, this mask can degrade naturally faster compared to conventional single use masks, so it is environmentally friendly. To implement this idea, there need to be a lot of party involved such as coconut seller, mask producers, medical equipment research team, and independent factories. Because its biodegradable materials, this mask will be a revolutionary invention in medical industry and will be very useful in preventing diseases while saving the environment.

Application of Gold Nanoparticles Synthesized with Malva Nut Gum as Reducing Agent for scFv Conjugates in a Colorimetric Chikungunya Virus Detection Muhamad Zulchoir Rasyid; Ratna Yulistya Ningrum; Sinta Setyaningrum; Budi Santoso; Muhammad Yusuf; Bachti Alisjahbana; Toto Subroto; Bevi Lidya
Fluida Vol 16 No 1 (2023): FLUIDA
Publisher : Department of Chemical Engineering, Politeknik Negeri Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35313/fluida.v16i1.4493

Gold nanoparticles have good surface plasmon properties in the presence of free electrons on their surface. The presence of free electrons on the surface of the plasmon causes gold nanoparticles to be able to bind to various kinds of biomolecules and proteins, including single-chain variable fragments (scFv). In this study, gold nanoparticle conjugates with Chikungunya antiviral scFv have been successfully prepared with the aim of developing a colorimetric method for rapid detection of envelope protein Chikungunya virus (E2 CHIKV). The manufacture of gold nanoparticles was carried out using malva nut gum and trisodium citrate as reducing agents. The results showed the formation of the AuNP-scFv conjugate which was characterized by a shift in the maximum wavelength for gold nanoparticles synthesized using malva nut gum as a reducing agent from 532.89 nm to 536.62 nm. Similarly, for gold nanoparticles synthesized using trisodium citrate as a reducing agent, the maximum wavelength shifted from 528.99 nm to 531.95 nm. The detection result of E2 CHIKV protein using the AuNP-scFv conjugate has not shown a positive result

Effect of Water Hyacinth’s Particle Size as Additional Substrate to the Leachate Anaerobic Bioreactor Laily Isna Ramadhani; Dewi Widyabudiningsih; Feri Wirawan; Aditya Almarnugraha; Sinta Setyaningrum
Fluida Vol 17 No 1 (2024): FLUIDA
Publisher : Department of Chemical Engineering, Politeknik Negeri Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35313/fluida.v17i1.5503

Municipal solid waste leachate contains landfill-produced organic contaminants. Leachate with the high organic content pottentially pollute nearby waterways. The high content of pollutants in leachate is represented by the measured Chemical Oxygen Demand (COD) content. Therefore, leachate was carried out using the anaerobic method. The anaerobic approach was chosen because it can process high-organic waste and produce biogas, in which methane in biogas may be used as renewable energy. Leachate from old landfills is heavy in nitrogen, thus to optimize the anaerobic process, it must be mixed with high-carbon substrates. Water hyacinth, a fast-growing plant with a high carbon content, is a weed because it pottentially damage the ecosystem. This research begins by comparing the anaerobic reactor with a single substrate of leachate and a mixed substrate (leachate and water hyacinth). Water hyacinth was added with size variations of 1 mm; 5 mm; 10 mm; and 15 mm. From the four variations, the mixed substrate reactor had a higher COD reduction efficiency than the single substrate in all water hyacinth sizes. The optimum particle size of water hyacinth is 1 mm, resulted the highest COD reduction efficiency of 81% and the highest biogas cummulative volume of 4,230 mL. The addition of water hyacinth as an additional substrate statisticaly proven has a strong correlation to the increasing efficiency of COD removal compared to the biogas production.

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