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Setyaningrum, Sinta
Jurusan Teknik Kimia, Politeknik Negeri Bandung
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Journal : Jurnal Teknik Kimia dan Lingkungan

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The Comparison of Free Cell and Immobilization Cell Fermentation on Bioethanol Production from Sorghum Stem by SSF and SHF Method Permanasari, Ayu Ratna; Syamfitri, Arum; Maharani, Devina; Setyaningrum, Sinta; Wibisono, Wahyu
Jurnal Teknik Kimia dan Lingkungan Vol. 8 No. 2 (2024): October 2024
Publisher : Politeknik Negeri Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33795/jtkl.v8i2.5755

Abstract

Bioethanol is a new and renewable energy source that can be produced from plants or crops containing sugars, starch, and lignocellulose. Sorghum stem contain a significant amount of sugar and lignocellulose. This study utilized sorghum stem in bioethanol production using Separated Hydrolysis and Fermentation (SHF) and Simultaneous Saccharification and Fermentation (SSF) methods. These two processes are generally used in the prouction of bioethanol with raw materials containing lignocellulose. However, raw materials derived from sweet sorghum stems has not been widely used in the previous studies. This study aimed to determine the effect of fermentation using free cell and cell immobilization techniques on various pH, as well as to identify the most optimal fermentation method (SHF or SSF) for producing the highest ethanol content in sorghum stem fermentation.  The fermentation was conducted at pH levels of 3, 4, and 5. Sorghum stem were processed into powder and followed by delingnification process by 3% of NaOH solution to degrade the lignin content. The hydrolysis process of sorghum stem used cellulase enzymes as the biocatalyst. Fermentation was carried out using Saacharomyce in term of dry yeast for 72 h. The results showed that the increasing within the range pH of 3-5 will increase the ethanol concentration. Freecell technique gave the better result over the immobilized. The best result reached out the ethanol concentration of 13.04 % by the SSF.
Co-Authors Aisyah Auliya Rahmawati Almarnugraha, Aditya Andrijanto, Eko Anindya Indrita Putri Annisaa Fitri Wulandari Asri Dwi Saffanah Bachti Alisjahbana Bevi Lidya Budi Santoso Dhea Nurul Amalia Dianti Devi Astari Heru Hermawan Iskandar, Aulia Syalwa Lili Indrawati Maharani, Devina Mohammad Taherzadeh Muhamad Zulchoir Rasyid Muhammad Yusuf Nancy Siti Djenar Ngatin, Agustinus Nurcahyo, Nurcahyo Paramitha, Tifa Permanasari, Ayu Ratna Raihan Nurfauziah Ramadhani, Laily Isna Ratna Yulistya Ningrum Regina J Patinvoh Riniati Ronny Purwadi Safitri, Dina Sania Gustiani Dewi Sudrajat Harris Abdulloh Suminar, Dian Ratna Syakira Athiya Tsany Syamfitri, Arum Toto Subroto WAHYU WIBISONO Wardana Putra, Adzikri Widyabudiningsih, Dewi Wirawan, Feri Zahra Nur Salsabila Zulfa Rahmah Fadillah