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All Journal e-Journal Pustaka Kesehatan STOMATOGNATIC- Jurnal Kedokteran Gigi Jurnal Kedokteran Gigi Universitas Padjadjaran Health Notions Journal of Dentomaxillofacial Science Journal Of Vocational Health Studies Meditory : The Journal of Medical Laboratory Indonesian Journal for Health Sciences (IJHS) Denta: Jurnal Kedokteran Gigi Odonto dental journal
Pudji Astuti
Bagian Biomedik Fakultas Kedokteran Gigi Universitas Jember Jember, Indonesia
Biochemistry, Genetics & Molecular Biology Dentistry Environmental Science Health Professions Library & Information Science Medicine & Pharmacology Nursing Public Health

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Viabilitas neutrofil yangdiinkubasi dalamekstrakrimpangtemulawak(Curcuma xanthorrhiza ) dan dipapar dengan Streptococcus mutans (Viability of neutrophil incubated in temulawak rhizome extract (Curcuma xanthorrhiza) and exposed by Streptococcus mutans) Ika Wahyu Purnamasari; Pudji Astuti; Tantin Ermawati
Journal of Dentomaxillofacial Science Vol. 13 No. 3 (2014): Formerly Jurnal Dentofasial ISSN 1412-8926
Publisher : DiscoverSys Inc.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15562/jdmfs.v13i3.403

Abstract

Streptococcus mutans are bacteria that have important role in caries development. When caries lesion is developing, itmay invade to deeper tissues such as dental pulp and initiate cellular defenses such as neutrophil. Neutrophil is type ofleukocytes which are important in phagocytosis process. If neutrophil fails in phagocytosis, neutrophil will be lysis andcause viability of neutrophil unprotected. Temulawak rhizome extract is predicted to increase viability of neutrophilbecause it contains curcuminoids, volatile oil, saponin, flavonoid, and tannin. This research aimed of this research wasto determine the viability of neutrophil exposed by S.mutans and incubated in temulawak rhizomes extract 2.5%, 5%,7.5%,10%, and 20%. Twenty four samples were divided into 6 groups, consisting of negative control, temulawak rhizomeextract 2.5%, 5%, 7.5%, 10%, and 20%. The viability of neutrophil was observed under an inverted microscope usingtrypan blue staining. This study concluded that temulawak rhizome extract 2.5%, 5%, 7.5%, and 10% could increaseviability of neutrophil exposed by S.mutans. Temulawak rhizome extract 5% has the effective concentration to increase viability of neutrophil.
Uji Antibakteri Ekstrak Daun Kersen (Muntingia Calabura L.) Terhadap Koloni Streptococcus viridans Akhmad Yusuf Sulaiman; Pudji Astuti; Amandia Dewi Permana Shita
Indonesian Journal for Health Sciences Vol 1, No 2 (2017)
Publisher : Universitas Muhammadiyah Ponorogo

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (3021.103 KB) | DOI: 10.24269/ijhs.v1i2.590

Abstract

Latar Belakang: Streptococcus viridans adalah salah satu bakteri patogen yang sering diisolasi dari saluran akar. Streptococcus viridans dapat menyebabkan endokarditis bakterial subakut pada manusia. Dokter gigi harus hati-hati dalam melakukan perawatan saluran akar. Irigasi merupakan salah satu tahapan dalam perawatan sluran akar. Bahan yang biasanya digunakan adalah sodium hipoklorit. Sodium hipoklorit dapat mengiritasi jaringan. Bahan alternatif diperlukan seperti daun kersen (Muntingia calabura L.). Daun kersen memilikikandungan aktif flavonoid, saponin, dan tanin yang berperan sebagai antibakteri. Tujuan penelitian: Untuk mengetahui daya antibakteri ekstrak daun kersen terhadap Streptococcus viridans dan besar konsentrasi ekstrak daun kersen yang memiliki daya hambat terbesar terhadap Streptococcus viridans. Metode: Penelitian ini terdiri dari 5 kelompok, yaitu K+ (sodium hipoklorit), P1 (ekstrak daun kersen 12,5%), P2 (ekstrak daun kersen 25%), P3 (ekstrak daun kersen 50%), P4 (ekstrak daun kersen 75%). Media BHI-A ditanami suspensi Streptococcus viridans. Selanjutnya kertas cakram yang sudah diberi larutan sesuai kelompoknya diletakkan di atas media BHI-A tersebut. Media diinkubasi selama 24 jam, lalu  diameter zona hambat diukur. Hasil dan Simpulan: Zona hambat terbesar yaitu pada kelompok P4 (ekstrak daun kersen 75%). Kesimpulan dari penelitian ini ekstrak daun kersen memiliki daya antibakteri terhadap Streptococcus viridans. Konsentrasi ekstrak daun kersen yang memiliki daya hambat terbesar terhadap Streptococcus viridans adalah sebesar 75%.
The Effect of Purple Leaf Extract (Graptophyllum pictum L. Griff) to The Amount of Fibroblast in Gingiva Rat Wistar induced by Porphyromonas gingivalis Savira Aulia Rachim; Atik Kurniawati; Pudji Astuti
Denta Journal Kedokteran Gigi Vol 14 No 2 (2020): Agustus
Publisher : Fakultas Kedokteran Gigi Universitas Hang Tuah

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30649/denta.v14i2.7

Abstract

Background: The prevalence of periodontal disease ranks second in dental and oral diseases after caries. Periodontitis is inflammation with bacteria infecting the host and involving all parts of the periodontal tissue. If this condition is left untreated, it can lead to fibrosis and irreversible damage. Various types of periodontitis therapy have not been able to provide optimal results in healing periodontitis and that therapy can cause side effects. Because of this background, the researchers wanted to carry out research on alternative treatments for periodontitis with purple leaves as an anti-inflammatory with an indicator of an increase in the number of fibroblast cells in inflamed areas. The use of purple leaves has been used since ancient times for the treatment of wounds and inflammation. Objective: To determine the effect of purple leaf extract (EDU) on increasing the number of fibroblast cells in the gingiva of Wistar rats infected by Porphyromonas gingivalis (Pg). Methods: 30 Wistar rats were divided into 5 groups, namely the normal group (KN), the control group Pg induced (K +) and the treatment group using EDU 2.5% (P1), EDU5% (P2), EDU10% (P3 ). All groups were induced by Pg except KN. EDU administration once a day for 7 days. On the 7th day the rats were decaputed and their gingivae were taken to make preparations and HE staining was carried out. Results readings in 3 different viewpoints were averaged and analyzed by one-way ANOVA. Results: The results showed that the K + group, 2.5% EDU, 5% EDU and 10% EDU groups increased the number of fibroblast significantly when compared to the Pg group (p < 0.05). Conclusion: EDU can increase the number of fibroblasts in Pg-induced mice
Penapisan fitokimia dan kandungan flavonoid total tanaman Calotropis gigantea: Studi eksperimental laboratoris Pudji Astuti; Zahara Meylawaty; Agustin Wulan Suci Dharmayanti; Sari Setyaningsih
Jurnal Kedokteran Gigi Universitas Padjadjaran Vol 35, No 2 (2023): Agustus 2023
Publisher : Fakultas Kedokteran Gigi Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/jkg.v35i2.47123

Abstract

ABSTRAKPendahuluan: Efek samping penggunaan obat analgesik anti-inflamasi dapat menyebabkan infeksi saluran cerna serius dan dapat berakibat fatal. Tanaman obat biduri (Calotropis gigantea) mempunyai kandungan flavonoid yang berkhasiat analgesik-antiinflamasi, dimana kualitas tanaman obat ditentukan oleh metabolit sekundernya. Kadar flavonoid dan senyawa fenolik lain di dalam tanaman berbeda–beda di setiap bagian, jaringan, dan umur tanaman. Tujuan penelitian ini untuk menganalisis penapisan fitokimia dan kadar flavonoid total dari berbagai bagian tanaman Calotropis gigantea. Metode: Jenis penelitian eksperimental laboratoris yaitu menggunakan ekstrak etanol dari daun, bunga, getah dan kulit akar tanaman Calotropis gigantea, kemudian dilakukan penapisan fitokimia untuk mengetahui adanya metabolit sekunder, seperti alkaloid, tannin, saponin, fenol, steroid dan terpenoid serta flavonoid. Selanjutnya dilakukan pengukuran kadar flavonoid total menggunakan spektrofotometer uv-vis. Data yang diperoleh dianalisis menggunakan independent T-test dengan nilai p=0,05. Hasil: Daun Calotropis gigantea mengandung tanin, saponin, fenol, steroid dan flavonoid; pada bunga mengandung tanin, fenol, steroid dan flavonoid; pada getah mengandung fenol, tannin, saponin dan steroid; pada kulit akar mengandung fenol, tannin, dan terpenoid. Flavonoid ditemukan pada daun dan bunga. Kadar flavonoid total berbeda signifikan antara daun dan bunga, dengan p=0,000<0,05. Simpulan: penapisan fitokimia pada daun, bunga, getah dan kulit akar Calotropis gigantea mengandung fenol, tannin dan steroid. Saponin terdapat pada daun dan getah, sedangkan flavonoid hanya terdapat pada daun dan bunga. Kadar flavonoid total pada daun lebih banyak daripada bunga.Kata kuncicalotropis gigantea, calotropis gigantea, flavonoid, penapisan, fitokimiaScreening of phytochemical and total flavonoid value of Calotropis gigantea  plant: Study eksperimental laboratorisIntroduction: Using anti-inflammatory analgesic drugs may lead to severe gastrointestinal infections that could be life-threatening. The medicinal plant Calotropis gigantea contains flavonoids, which possess analgesic and anti-inflammatory properties. The quality of this medicinal plant is contingent on its secondary metabolites. The levels of flavonoids and other phenolic compounds within the plant vary across different parts, tissues, and developmental stages. This study aimed to conduct a phytochemical screening and assess the total flavonoid content in various components of the Calotropis gigantea plant. Method: This laboratory experimental research involves utilizing ethanol extracts from the leaves, flowers, sap, and root bark of the Calotropis gigantea plant. The objective is to conduct a phytochemical screening to identify the presence of secondary metabolites, including alkaloids, tannins, saponins, phenols, steroids, terpenoids, and flavonoids. Subsequently, the total flavonoid content is measured using a UV-vis spectrophotometer. The collected data undergo analysis using an independent t-test, with a significance level set at p = 0.05. Results: show that Calotropis gigantea leaves contain tannins, saponins, phenols, steroids, and flavonoids; flowers contain tannins, phenols, steroids, and flavonoids; the latex contains phenols, tannins, saponins, and steroids; and the root bark contains phenols, tannins, and terpenoids. Flavonoids are present in both leaves and flowers. Notably, the total flavonoid levels exhibited significant differences between leaves and flowers, with a p-value of 0.00 < 0.05. Conclusion: Phytochemical screening of the leaves, flowers, sap, and root bark of Calotropis gigantea revealed the presence of phenols, tannins, and steroids. Saponins were identified in leaves and sap, while flavonoids were exclusively detected in leaves and flowers. Furthermore, the leaves' total flavonoid content was higher than the flowers.Keywordscalotropis gigantea , calotropis gigantea, flavonoids, phytochemical, screening
Median rhomboid glossitis as a sign of undiagnosed diabetes mellitus - a case report Erna Sulistyani; Iin Eliana Triwahyuni; Pudji Astuti; Rindang Swandari
Odonto : Dental Journal Vol 10, No 1 (2023): July 2023
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30659/odj.10.1.7-13

Abstract

Background: Median Rhomboid Glossitis (MRG) is included in the group of nodular-plaque-like candidiasis, a subtype of chronic hyperplastic candidiasis. A lot of literature suggests that MRG is a manifestation of Diabetes Mellitus (DM), but the association between DM and MRG is unclear until now. This article reports an MRG found in an undiagnosed diabetes mellitus patient. Case: In October 2019, A 71-year-old man came to RSGM Universitas Jember to fix his loose denture. In the oral examination, we found a well-bordering, atrophic oval area surrounded by thick- soft white plaque. In his leg, we found itchy papule, ulcer, and crust multiple lesions. The microscopic examination of the white plaque swab showed that the spore and hyphae were found to fill all fields of view. The direct sugar blood level test showed that the sugar blood level was 390 mg/dL. We diagnosed the patient as having an MRG associated with suspected DM. Case Management: The patient was given miconazole oral gel and recommended to use it after cleaning the tongue three times a day. For diabetes management, the patient was referred to the Unit Medical Center University of Jember. The patient was diagnosed with diabetes mellitus and given oral-antidiabetics. After two weeks, the atrophic area had improved, and the white plaque had disappeared. Conclusion: General practitioners must be aware if they find the MRG in their patients caused a probability of underlying undiagnosed systemic disease, particularly diabetes mellitus on them.
MINIMUM INHIBITORY CONCENTRATION OF PURPLE LEAF EXTRACT (GRAPTOPHYLLUM PICTUM L. GRIFF) AGAINST LACTOBACILLUS ACIDOPHILUS ATCC 4356 Indriyani Chusnadia, Shafira; Kurniawati, Atik; Setyaningsih, Sari; Wulandari, Erawati; Astuti, Pudji
Journal of Vocational Health Studies Vol. 7 No. 2 (2023): November 2023 | JOURNAL OF VOCATIONAL HEALTH STUDIES
Publisher : Faculty of Vocational Studies, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jvhs.V7.I2.2023.115-120

Abstract

Background: Dental caries is an oral cavity disease that affects most Indonesians. Lactobacillus acidophilus (L. acidophilus) is one of the bacteria that causes dental caries. Control of bacteria in the form of antibacterial agents is needed to suppress the growth of L. acidophilus. Purple leaves (Graptophyllum pictum L. Griff) are a medicinal plant with antibacterial compounds, namely flavonoids, alkaloids, saponins, and tannins. Purpose: Determine the Minimum Inhibitory Concentration (MIC) of purple leaves extract on the growth of L. acidophilus. Method: The sample consisted of seven groups, including positive control (chlorhexidine 0.2%), negative control (BHI-B), and purple leaves extract with concentrations of 25%, 12.5%, 6.25%, 3.12%, and 1.56%. The antibacterial activity of purple leaves extract was carried out quantitatively using a spectrophotometer with a wavelength of 600 nm. After that, it was incubated at 37°C for 48 hours, followed by absorbance measurement. The absorbance results were then analyzed using the Paired T-Test (before and after incubation). Result: Purple leaves extract concentrations of 6.25%, 12.5%, and 25% had an inhibitory effect on L. acidophillus. Conclusion: Minimum Inhibitory Concentration (MIC) of purple leaves extract on the growth of L. acidophilus was 6.25%
BIOVIABILITY OF BIDURI LEAF EXTRACT (Calotropis Gigantea L.) ON FIBROBLAST CELL CULTURE Sari Setyaningsih; Pudji Astuti; Zahara Meilawaty; Agustin Wulan Suci Dharmayanti
Meditory : The Journal of Medical Laboratory Vol 13, No 1 (2025): Meditory, Volume 13 No. 1 Tahun 2025
Publisher : Jurusan Teknologi Laboratorium Medis, Poltekkes Kemenkes Denpasar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33992/meditory.v13i1.4049

Abstract

Background: Biduri is recognized for its medicinal properties, particularly in wound healing. The active compounds influence the number of fibroblast cells, which play a role in wound healing. Research on the bioviability of biduri leaf extract has never been conducted, so in vitro testing is necessary to determine the ability of biduri leaf extract to influence fibroblast cells.Objective:  To assess the effect of Biduri leaf extract on the viability of BHK-21 fibroblast cells in vitro. Methods: This experiment used a posttest-only group Design with an MTT assay to assess the viability of BHK-21 fibroblast cells after exposure to different concentrations of Biduri leaf extract. The study employed four treatment groups with varying extract concentrations and used one-way ANOVA followed by an LSD test to analyze the proportion of living cells statistically.Results:  The viability percentages of fibroblast cells varied with the concentration of Biduri leaf extract. The highest viability was observed at 5% concentration (80.82%), while the lowest was at 20% concentration (60.25%). A one-way ANOVA test was used to analyze the data, and differences were considered statistically significant at p 0.05..Conclusions: The highest viability was observed at 5%, while the lowest was at 20% concentration. All treatment groups had cell viability above 60%, indicating that the Biduri extract is generally biocompatible with fibroblast cell culture.
Co-Authors Abdul Rochim Abdul Rochim Agustin Wulan Suci Dharmayanti Akhmad Yusuf Sulaiman Amandia Dewi Permana Shita Amandia Dewi Permana Shita Atik Kurniawati Berlian Prihatiningrum Danang Dewantara Ananda Putra Dwi Warna Aju Fatmawati Erawati Wulandari Erna Sulistyani Hardhika Oktarianda Fachri Hestieyonini Hadnyanawati I Dewa Ayu Ratna Dewanti Iin Eliana Triwahyuni Ika Wahyu Purnamasari Indah Widyanti Indriyani Chusnadia, Shafira Julia Eka Putri Ayuningtyas Lutfi Meiga Sari Melok Aris Wahyukundari Narita Ajeng Loviana Rendra Chriestedy Prasetya Resti Ayu Indriana Rindang Swandari Ristya Widi Endah Yani Rosanita Firdausi Oktaviani Rr. Nektara Titan Dianastri Sari Setyaningsih Savira Aulia Rachim Sri Lestari Tantin Ermawati Winny Adriatmoko Zahara Meilawaty Zahara Meylawaty Zahreni Hamzah