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PENGARUH KALSIUM DAN VITAMIN D TERHADAP RISIKO TERJADINYA KANKER PROSTAT Sari Setyaningsih
Medika Kartika : Jurnal Kedokteran dan Kesehatan Vol 5 No 2 (2022): Medika Kartika : Jurnal Kedokteran dan Kesehatan
Publisher : Fakultas Kedokteran Universitas Jenderal Achmad Yani

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (282.805 KB)

Kalsium merupakan unsur penting yang mengontrol berbagai aktivitas tubuh. Asupan kalsium yang tinggi, ternyata dapat meningkatkan risiko kanker prostat, khususnya meningkatkan proliferasi sel kanker prostat. Kalsium berperan sebagai regulator 1,25 dihidroksi vitamin D. Selain itu, kalsium juga berperan sebagai regulator Parathyroid Hormone (PTH), kalsium serum yang tinggi akan menurunkan PTH dalam sirkulasi sehingga konversi 25(OH) vitamin D menjadi vitamin D aktif dengan bantuan PTH juga menurun. Kedua hal ini menyebabkan penurunan regulasi 1,25 dihidroksi vitamin D, vitamin D aktif yang diduga berperan penting dalam proses karsinogenesis melalui inhibisi pertumbuhan dan proliferasi sel kanker dan metastasis. Proses ini disebabkan ketika VDR (Vitamin D Receptor) tidak berikatan dengan ligannya dapat meregulasi ekspresi gen yang berfungsi dalam memicu proliferasi, angiogenesis dan menghambat terjadinya apoptosis. Mekanisme kalsium dalam risiko terjadinya kanker prostat juga berkaitan dengan peranan CaSR (Calcium-Sensing Receptor). CaSR merupakan reseptor yang ditemukan di berbagai organ, salah satunya adalah pada kelenjar prostat. Asupan kalsium yang tinggi dapat meningkatkan peningkatan reseptor dan memacu terjadinya proliferasi sel-sel prostat. DOI : 10.35990/mk.v5n2.p195-207

Penapisan fitokimia dan kandungan flavonoid total tanaman Calotropis gigantea: Studi eksperimental laboratoris Pudji Astuti; Zahara Meylawaty; Agustin Wulan Suci Dharmayanti; Sari Setyaningsih
Jurnal Kedokteran Gigi Universitas Padjadjaran Vol 35, No 2 (2023): Agustus 2023
Publisher : Fakultas Kedokteran Gigi Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/jkg.v35i2.47123

ABSTRAKPendahuluan: Efek samping penggunaan obat analgesik anti-inflamasi dapat menyebabkan infeksi saluran cerna serius dan dapat berakibat fatal. Tanaman obat biduri (Calotropis gigantea) mempunyai kandungan flavonoid yang berkhasiat analgesik-antiinflamasi, dimana kualitas tanaman obat ditentukan oleh metabolit sekundernya. Kadar flavonoid dan senyawa fenolik lain di dalam tanaman berbeda–beda di setiap bagian, jaringan, dan umur tanaman. Tujuan penelitian ini untuk menganalisis penapisan fitokimia dan kadar flavonoid total dari berbagai bagian tanaman Calotropis gigantea. Metode: Jenis penelitian eksperimental laboratoris yaitu menggunakan ekstrak etanol dari daun, bunga, getah dan kulit akar tanaman Calotropis gigantea, kemudian dilakukan penapisan fitokimia untuk mengetahui adanya metabolit sekunder, seperti alkaloid, tannin, saponin, fenol, steroid dan terpenoid serta flavonoid. Selanjutnya dilakukan pengukuran kadar flavonoid total menggunakan spektrofotometer uv-vis. Data yang diperoleh dianalisis menggunakan independent T-test dengan nilai p=0,05. Hasil: Daun Calotropis gigantea mengandung tanin, saponin, fenol, steroid dan flavonoid; pada bunga mengandung tanin, fenol, steroid dan flavonoid; pada getah mengandung fenol, tannin, saponin dan steroid; pada kulit akar mengandung fenol, tannin, dan terpenoid. Flavonoid ditemukan pada daun dan bunga. Kadar flavonoid total berbeda signifikan antara daun dan bunga, dengan p=0,000<0,05. Simpulan: penapisan fitokimia pada daun, bunga, getah dan kulit akar Calotropis gigantea mengandung fenol, tannin dan steroid. Saponin terdapat pada daun dan getah, sedangkan flavonoid hanya terdapat pada daun dan bunga. Kadar flavonoid total pada daun lebih banyak daripada bunga.Kata kuncicalotropis gigantea, calotropis gigantea, flavonoid, penapisan, fitokimiaScreening of phytochemical and total flavonoid value of Calotropis gigantea plant: Study eksperimental laboratorisIntroduction: Using anti-inflammatory analgesic drugs may lead to severe gastrointestinal infections that could be life-threatening. The medicinal plant Calotropis gigantea contains flavonoids, which possess analgesic and anti-inflammatory properties. The quality of this medicinal plant is contingent on its secondary metabolites. The levels of flavonoids and other phenolic compounds within the plant vary across different parts, tissues, and developmental stages. This study aimed to conduct a phytochemical screening and assess the total flavonoid content in various components of the Calotropis gigantea plant. Method: This laboratory experimental research involves utilizing ethanol extracts from the leaves, flowers, sap, and root bark of the Calotropis gigantea plant. The objective is to conduct a phytochemical screening to identify the presence of secondary metabolites, including alkaloids, tannins, saponins, phenols, steroids, terpenoids, and flavonoids. Subsequently, the total flavonoid content is measured using a UV-vis spectrophotometer. The collected data undergo analysis using an independent t-test, with a significance level set at p = 0.05. Results: show that Calotropis gigantea leaves contain tannins, saponins, phenols, steroids, and flavonoids; flowers contain tannins, phenols, steroids, and flavonoids; the latex contains phenols, tannins, saponins, and steroids; and the root bark contains phenols, tannins, and terpenoids. Flavonoids are present in both leaves and flowers. Notably, the total flavonoid levels exhibited significant differences between leaves and flowers, with a p-value of 0.00 < 0.05. Conclusion: Phytochemical screening of the leaves, flowers, sap, and root bark of Calotropis gigantea revealed the presence of phenols, tannins, and steroids. Saponins were identified in leaves and sap, while flavonoids were exclusively detected in leaves and flowers. Furthermore, the leaves' total flavonoid content was higher than the flowers.Keywordscalotropis gigantea , calotropis gigantea, flavonoids, phytochemical, screening

MINIMUM INHIBITORY CONCENTRATION OF PURPLE LEAF EXTRACT (GRAPTOPHYLLUM PICTUM L. GRIFF) AGAINST LACTOBACILLUS ACIDOPHILUS ATCC 4356 Indriyani Chusnadia, Shafira; Kurniawati, Atik; Setyaningsih, Sari; Wulandari, Erawati; Astuti, Pudji
Journal of Vocational Health Studies Vol. 7 No. 2 (2023): November 2023 | JOURNAL OF VOCATIONAL HEALTH STUDIES
Publisher : Faculty of Vocational Studies, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jvhs.V7.I2.2023.115-120

Background: Dental caries is an oral cavity disease that affects most Indonesians. Lactobacillus acidophilus (L. acidophilus) is one of the bacteria that causes dental caries. Control of bacteria in the form of antibacterial agents is needed to suppress the growth of L. acidophilus. Purple leaves (Graptophyllum pictum L. Griff) are a medicinal plant with antibacterial compounds, namely flavonoids, alkaloids, saponins, and tannins. Purpose: Determine the Minimum Inhibitory Concentration (MIC) of purple leaves extract on the growth of L. acidophilus. Method: The sample consisted of seven groups, including positive control (chlorhexidine 0.2%), negative control (BHI-B), and purple leaves extract with concentrations of 25%, 12.5%, 6.25%, 3.12%, and 1.56%. The antibacterial activity of purple leaves extract was carried out quantitatively using a spectrophotometer with a wavelength of 600 nm. After that, it was incubated at 37°C for 48 hours, followed by absorbance measurement. The absorbance results were then analyzed using the Paired T-Test (before and after incubation). Result: Purple leaves extract concentrations of 6.25%, 12.5%, and 25% had an inhibitory effect on L. acidophillus. Conclusion: Minimum Inhibitory Concentration (MIC) of purple leaves extract on the growth of L. acidophilus was 6.25%

KEMAMPUAN GEL EKSTRAK BIJI KOPI ROBUSTA (Coffea canephora) DALAM MENURUNKAN TRAP PADA TIKUS MODEL PERIODONTITIS YANG DIINDUKSI KOMBINASI LPS P. gingivalis DAN WIRE NITI Rachmawati, Dessy; Setyaningsih, Sari; Herniyati; Rahmah, Tanzilal Azizir
Jurnal Kesehatan Vol 17 No 2 (2024): JURNAL KESEHATAN
Publisher : Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/kesehatan.v17i2.39781

Periodontitis is an inflammatory disease of the tooth supporting tissues caused by pathogenic microorganisms in dental plaque. P. gingivalis as the main etiology of periodontitis has a virulence factor in the form of lipopolysaccharide (LPS). The use of NiTi wires in initial orthodontic treatment can exacerbate periodontitis. Robusta coffee beans contain active compounds such as chlorogenic acid, caffeic acid, ferulic acid and caffeine as anti-inflammatories and antioxidants. This study aims to analyze the ability of robusta coffee bean extract gel to reduce TRAP in a rat model of periodontitis induced by the combination of LPS P. gingivalis and NiTi wire. The 28 male wistar rats were classified into 7 groups of normal (K), injected with LPS (P1), NiTi wire (P2), combination of LPS and NiTi wire (P3), injected with LPS and 500 mg/ml robusta coffee bean gel (P4), NiTi wire and 500 mg/ml robusta coffee bean gel (P5), combination of LPS and NiTi wire with 500 mg/ml robusta coffee bean gel (P6). The rats were decapitated, and then the tissue was stained with HE and IHC. The expression of TRAP was counted in the area around alveolar bone of the lower-left M1. Data were analyzed using SPSS software by going through several test, such as Saphiro-Wilk, Levene-Test, Kruskal-Wallis, and followed by Mann-Whitney. The results of TRAP expression showed a significant decreased (p<0,05) on combination of LPS and NiTi wire with robusta coffee bean extract gel. Robusta coffee bean extract gel was able to reduce TRAP expression on periodontitis model rats induced by the combination of LPS P. gingivalis and NiTi wire.

Uji aktivitas antibakteri dan cemaran mikroba daun biduri (Calotropis gigantea l.) sesuai standar mutu bahan baku obat herbal: studi eksperimental laboratoris Setyaningsih, Sari; Astuti, Pudji; Meilawaty, Zahara; Dharmayanti, Agustin Wulan Suci; Ratna Dewanti, I Dewa Ayu; Yunianti, Ervisya Nandya
Jurnal Kedokteran Gigi Universitas Padjadjaran Vol 36, No 3 (2024): Desember 2024
Publisher : Fakultas Kedokteran Gigi Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/jkg.v36i3.58528

Pendahuluan. Daun biduri (Calotropis gigantea) digunakan oleh masyarakat sebagai obat tradisional khususnya mengobati sakit gigi. Ekstrak daun biduri diketahui memiliki kandungan senyawa flavonoid, triterpene glycosides, abrin, dan alkaloids. Salah satu syarat untuk menjadikan tanaman obat sebagai sediaan farmasi diperlukan standarisasi cemaran mikroba dan uji antibakteri. Tujuan penelitian ini untuk menganalisis aktivitas antibakteri dan cemaran mikroba daun biduri. Metode: Uji cemaran mikroba menggunakan metode angka lempeng total bakteri dan kapang. Sedangkan uji aktivitas antibakteri menggunakan metode difusi cakram dengan konsentrasi ekstrak daun biduri 15%, 20%, 25% dan 30% terhadap bakteri Streptococcus mutans dan Porphyromonas gingivalis. Data untuk uji aktivitas antibakteri dianalisis menggunakan Anova One Way sedangkan untuk uji cemaran mikroba menggunakan metode deskriptif kuantitatif dibandingkan dengan standar cemaran mikroba berdasarkan Peraturan Kepala Bidang Pengawas Obat dan Makanan (BPOM). Hasil: Uji antibakteri terhadap S. mutans menunjukkan hasil zona hambat kategori sedang, dengan rata-rata diameter zona hambat dari kontrol negatif (0 mm), konsentrasi 15% (8,94 mm), 20% (9,05 mm), 25% (9,65 mm), 30% (9,79 mm), dan kontrol positif (22,43 mm). Sedangkan pada P. gingivalis tidak ada zona hambat. Hasil uji statistik didapatkan nilai signifikansi p=0,00 (p<0,05). Hasil uji cemaran mikroba menunjukkan hasil perhitungan jumlah bakteri 13.300 cfu/gram dan tidak ditemukan pertumbuhan kapang. Simpulan: Ekstrak daun biduri memiliki daya hambat terhadap Streptococcus mutans, tetapi tidak memiliki daya hambat terhadap Porphyromonas gingivalis. Cemaran ekstrak daun biduri masih memenuhi syarat batas cemaran sesuai standar bahan baku obat herbal.Antibacterial Activity Test and Microbial Contamination of Biduri Leaves (Calotropis gigantea L.) according to Quality Standards for Herbal Medicine: Experimental Laboratory StudiIntroduction. Biduri leaves (Calotropis gigantea) are used by the community as traditional medicine, especially to treat toothache. Biduri leaf extract is known to contain flavonoid, triterpene glycosides, abrin, and alkaloids. One of the requirements for making medicinal plants as pharmaceutical preparations requires standardization of microbial contamination and antibacterial test. Objective: to analyze the antibacterial activity and microbial contamination of biduri leaves. Method: The microbial contamination test uses the total bacterial and fungal plate count method. While the antibacterial activity test uses the disc diffusion method on 15%, 20%, 25% and 30% biduri leaf extract against Streptococcus mutans and Porphyromonas gingivalis. Data for antibacterial activity test were analyzed using Anova One Way while for microbial contamination test using quantitative descriptive method and compared with microbial contamination in accordance with Regulation of Head of Drug and Food Supervisory Division (BPOM). Results: Antibacterial test against S. mutans showed moderate inhibition zone result, with average diameter of inhibition zone from negative control (0 mm), concentration 15% (8,94 mm), 20% (9,05 mm), 25% (9,65 mm), 30% (9,79 mm), and positive control (22,43 mm). Meanwhile, in P. gingivalis there is no inhibition zone. Statistical test result obtained significance value p=0.00 (p<0.05). Microbial contamination test result showed bacterial count result 13,300 cfu/gram and no mold growth was found. Conclusion: Biduri leaf extract has inhibition against S. mutans, but has no inhibition against P. gingivalis. The contamination of Biduri leaf extract still meets the contamination limit according to the standard of herbal medicinal raw materials.

Uji efektivitas antibakteri daun Biduri (Calotropis gigantea) terhadap pertumbuhan Streptococcus mutans: studi eksperimental laboratoris Widodo, Alaya Dwi Salvahira; Setyaningsih, Sari; Astuti, Pudji; Meilawaty, Zahara; Suci Dharmayanti, Agustin Wulan
Jurnal Kedokteran Gigi Universitas Padjadjaran Vol 36, No 3 (2024): Desember 2024
Publisher : Fakultas Kedokteran Gigi Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/jkg.v36i3.56148

Pendahuluan: Karies merupakan penyakit jaringan keras pada gigi yang disebabkan oleh asam hasil bakteri plak yang menumpuk pada permukaan gigi. Salah satu bakteri plak adalah Streptococcus mutans. Penggunaan obat kumur Chlorhexidine gluconate 0,2% terbukti mampu menghambat S.mutans. Namun penggunaan Chlorhexidine gluconate 0,2% dalam jangka panjang dapat menimbulkan efek samping. Oleh karena itu, diperlukan bahan alami seperti tanaman biduri yang memiliki efek antibakteri untuk menghindari efek samping tersebut. Penelitian ini bertujuan menganalisis ekstrak daun biduri (Calotropis gigantea) dapat menghambat pertumbuhan S.mutans. Metode: Jenis penelitian eksperimental laboratorium. Penelitian ini menggunakan 6 kelompok perlakuan yaitu kelompok perlakuan ekstrak daun biduri dengan konsentrasi 15%, 20%, 25%, 30%, K(-) akuades, dan K(+)Chlorhexidine gluconate 0,2%. Kelompok perlakuan dan kelompok kontrol dipaparkan S.mutans untuk melihat efek antibakterinya dan menggunakan metode spektrofotometri UV-Vis dan hitung koloni bakteri (total plate count). Hasil: Hasil penelitian menunjukkan, masing-masing konsentrasi menunjukkan hasil laju penghambatan sebesar 0 pada kelompok K(-), 45,78% pada kelompok 15%, 34,93% pada kelompok 20%, 76,5% pada kelompok 25%, 64,45% pada kelompok 30%, dan 89,45% pada kelompok K(+). Hasil hitung koloni bakteri penghambatan bakteri terbesar yaitu pada konsentrasi 25%. Hasil yang diperoleh dilakukan analisis data. Data sebelum dan sesudah inkubasi dilakukan uji Paired T Test dan menunjukkan perbedaan yang signifikan. Analisis data laju penghambatan dilakukan dengan uji Kruskal Wallis dilanjutkan uji Mann Whitney Ekstrak daun biduri konsentrasi 15% dibandingkan dengan konsentrasi 20% dan 30% dibandingkan dengan 25% memiliki nilai p>0,05 sehingga tidak memiliki perbedaan yang bermakna. Simpulan: Daun biduri efektif menghambat pertumbuhan S.mutans.Antibacterial effectiveness test of biduri leaf (Calotropis gigantea) against Streptococcus mutans: experimental laboratory studyIntroduction: Caries is a disease that affects the hard tissue of the teeth, caused by acid deposits produced by plaque bacteria that accumulate on the tooth surface. One of the plaque bacteria is Streptococcus mutans. Chlorhexidine gluconate 0.2% mouthwash has been shown to inhibit S. mutans. However, prolonged use of Chlorhexidine gluconate 0.2% mouthwash can cause side effects. To avoid these side effects, natural ingredients like biduri plant that have antibacterial effects are needed. This research aims to analyze the inhibitory effects of biduri (Calotropis gigantea) leaf extract on the growth of S. mutans. Methods: This research is experimental laboratory study. Six treatment groups were used, four groups treated with biduri leaf extract at concentrations of 15%, 20%, 25%, 30%; the K(-) using distilled water,; and K(+) using Chlorhexidine gluconate 0.2%. The treatment and control group were exposed to S. mutans to evaluate its antibacterial effect and assessed using the UV-Vis spectrophotometric and total plate count method. Results: In the research conducted, each concentration showed varying inhibition rates. The inhibition rates observed were as follows: 0% for the K(-), 45,78% for the 15% group, 34,93% for the 20% group, 76,5% for the 25% group, 64,45% for the 30% group, and 89,45% for the K(+). In bacterial colony count, the greatest bacterial inhibition was observed at concentration of 25%. Statistical analysis was performed on the obtained data. A Paired T-Test demonstrated a significant difference between the data before and after incubation. Further analysis using the Kruskal Wallis and Mann Whitney tests revealed no significant difference in inhibition rates between 15% and 25% concentrations, or between 25% and 30%. Conclusion: Biduri leaf extract effectively inhibits the growth of S. mutans.

Examining the Impact of Porphyromonas gingivalis Infection on Ovarian Health: A Reduction in Primordial Follicles in a Female Periodontitis Model Meilawaty, Zahara; Galau, Argha Sirna; Permatasari, Sabania Dinar; Setyaningsih, Sari; Astuti, Pudji; Setiawatie, Ernie Maduratna; Hendarto, Hendy; Suci Dharmayanti, Agustin Wulan
Jurnal Kesehatan Gigi Vol 12, No 1 (2025): Juni 2025
Publisher : Jurusan Kesehatan Gigi, Poltekkes Kemenkes Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31983/jkg.v12i1.12547

Periodontitis is a chronic inflammation of the periodontal tissues caused by periodontal pathogens, such as Porphyromonas gingivalis (P. gingivalis). P. gingivalis possesses several virulence factors that enable it to invade and spread through the bloodstream to other organs, including the ovaries. It triggers inflammation, which may impact the number of primordial follicles. The aim of this study was to assess the effects of P. gingivalis infection on the number of primordial follicles in the ovaries. This study was a laboratory experiment with a post-test control group design. The rats were divided into control and periodontitis groups. The periodontitis group consisted of rats induced with P. gingivalis in their sulcus gingiva of the first mandible molar every three days for 19 days. Therefore, control groups were without P. gingivalis induction. Seven days after the last induction of P. gingivalis, the rats were euthanized, and their ovarian tissues were collected for histological preparation. The primordial follicles were counted under 400x magnification. The results indicated that the treatment group had a significantly lower number of primordial follicles (2.2 ± 2.61) compared to the control group (12.4 ± 2.41). The study concludes that P. gingivalis infection led to a reduction in the number of ovarian primordial follicles in periodontitis models.

Ekstrak daun biduri (Calotropis gigantea) sebagai anti inflamasi dalam menurunkan jumlah neutrofil pada model tikus inflamasi: studi eksperimental Permata, Dea; Meilawaty, Zahara; Astuti, Pudji; Dharmayanti, Agustin Wulan Suci; Setyaningsih, Sari; Prasetya, Rendra Chriestedy
Jurnal Kedokteran Gigi Universitas Padjadjaran Vol 37, No 2 (2025): Agustus 2025
Publisher : Fakultas Kedokteran Gigi Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/jkg.v37i2.61210

Pendahuluan: Inflamasi adalah respons imun terhadap rangsangan berbahaya yang bertujuan untuk menghilangkan kerusakan dan memulai penyembuhan. Proses inflamasi dimulai dengan pelepasan neutrofil yang mengatur dan memperkuat respon inflamasi namun. Obat Anti-Inflamasi Non-Steroid (NSAID), seperti aspirin, sering digunakan sebagai anti inflamasi tetapi memiliki efek samping. Ekstrak daun biduri (Calotropis gigantea) yang kaya akan flavonoid, tanin, dan saponin, yang memiliki sifat anti-inflamasi dan penyembuhan luka dengan efek samping yang lebih sedikit dapat digunakan sebagai obat alami alternatif. Penelitian ini bertujuan untuk menganalisis perbedaan kemampuan ekstrak daun biduri (Calotropis gigantea) dalam menurunkan jumlah neutrofil pada inflamasi akut dan subakut pada model tikus inflamasi. Metode: Penelitian ini adalah studi eksperimental in vivo menggunakan desain serial group design. Daun biduri diekstrak menggunakan metode maserasi dengan pelarut etanol 70%. Dosis ekstrak daun biduri yang digunakan adalah 100 mg/kg BB, 200 mg/kg BB, dan 300 mg/kg BB. Tikus wistar diinjeksi dengan karagenan 2% pada punggungnya secara subkutan sebagai model inflamasi. Sampel darah diambil pada interval tertentu untuk membuat hapusan darah, selanjutnya dilakukan penghitungan jumlah neutrofil. Analisis menggunakan Two-way Anova yang dilanjutkan dengan uji LSD. Hasil: Ekstrak daun Calotropis gigantea pada dosis 100 mg/Kg BB, 200 mg/Kg BB, dan 300 mg/Kg BB terbukti dapat mengurangi jumlah neutrofil, terutama pada hari ke-3, dengan dosis 300 mg/Kg BB memberikan hasil terbaik (P=0,001). Simpulan: Pemberian ekstrak daun biduri (Calotropis gigantea) dosis 300 mg/Kg BB memberikan hasil terbaik dalam menurunkan jumlah sel neutrofil pada inflamasi akut dan subakut pada model tikus inflamasi.Calotropis gigantea leaf extract as an anti-inflammatory agent in reducing neutrophil count in an inflammatory rat model: an experimental studyIntroduction: Inflammation is an immune response to harmful stimuli that serves to eliminate tissue damage and initiate healing. The inflammatory process begins with the release of neutrophils, which regulate and amplify the inflammatory response. Non-steroidal anti-inflammatory drugs (NSAIDs), such as aspirin, are commonly used to treat inflammation but may cause side effects. Biduri leaf (Calotropis gigantea)extract which is rich in flavonoids, tannins, and saponins, possesses anti-inflammatory and wound-healing properties with fewer side effects, making it a potential natural alternative medicine. This study aims to evaluate the effectiveness of biduri leaf extract (Calotropis gigantea) in reducing the number of neutrophils during acute and subacute inflammation in an experimental rat model. Methods: This study is an in vivo experimental study using a serial group design. Biduri leaves were extracted using the maceration method with 70% ethanol as the solvent. The biduri leaf extract was administered at doses of 100 mg/kg BW, 200 mg/kg BW, and 300 mg/kg BW. Wistar rats were injected subcutaneously with 2% carrageenan on the dorsal region to induce inflammation. Blood samples were collected at predetermined intervals to prepare blood smears, followed by neutrophil counting. Data were analyzed using two-way ANOVA followed by the LSD post-hoc test. Results: Calotropis gigantea leaf extract at doses of 100 mg/Kg BW, 200 mg/Kg BW, and 300 mg/Kg BW significantly reduced neutrophil counts, particularly on day 3, with the 300 mg/Kg BW dose showing the most pronounced effect (p=0.001). Conclusion: Administration of biduri leaf (Calotropis gigantea) extract at a dose of 300 mg/Kg BW demonstrated the highest effectiveness in reducing neutrophil count during acute and subacute inflammation in an inflammation rat model.

BIOVIABILITY OF BIDURI LEAF EXTRACT (Calotropis Gigantea L.) ON FIBROBLAST CELL CULTURE Setyaningsih, Sari; Astuti, Pudji; Meilawaty, Zahara; Dharmayanti, Agustin Wulan Suci
Meditory : The Journal of Medical Laboratory Vol 13, No 1 (2025): Meditory, Volume 13 No. 1 Tahun 2025
Publisher : Jurusan Teknologi Laboratorium Medis, Poltekkes Kemenkes Denpasar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33992/meditory.v13i1.4049

Background: Biduri is recognized for its medicinal properties, particularly in wound healing. The active compounds influence the number of fibroblast cells, which play a role in wound healing. Research on the bioviability of biduri leaf extract has never been conducted, so in vitro testing is necessary to determine the ability of biduri leaf extract to influence fibroblast cells.Objective: To assess the effect of Biduri leaf extract on the viability of BHK-21 fibroblast cells in vitro. Methods: This experiment used a posttest-only group Design with an MTT assay to assess the viability of BHK-21 fibroblast cells after exposure to different concentrations of Biduri leaf extract. The study employed four treatment groups with varying extract concentrations and used one-way ANOVA followed by an LSD test to analyze the proportion of living cells statistically.Results: The viability percentages of fibroblast cells varied with the concentration of Biduri leaf extract. The highest viability was observed at 5% concentration (80.82%), while the lowest was at 20% concentration (60.25%). A one-way ANOVA test was used to analyze the data, and differences were considered statistically significant at p 0.05..Conclusions: The highest viability was observed at 5%, while the lowest was at 20% concentration. All treatment groups had cell viability above 60%, indicating that the Biduri extract is generally biocompatible with fibroblast cell culture.

Toothbrush design appropriate for pre-elderly: The relationship of toothbrush bristles number to oral hygiene Suhartini, Suhartini; Hamzah, Zahreni; Setyaningsih, Sari; Prijatmoko, Dwi; Harmono, Happy; Arina, Yuliana Mahdiyah Daat; Prihatiningrum, Berlian
Odonto : Dental Journal Vol 11, No 1 (2024): July 2024
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30659/odj.11.1.%p

Background: The increase in the elderly population leads to a decline in their health and affects the productivity and quality of life. Preventive measures must be taken to improve oral hygiene for the elderly starting from the pre-elderly group so that later it will form healthy and productive elderly. One of the efforts to improve dental hygiene and health is through mechanical tooth cleaning with a toothbrush. However, currently, there is no toothbrush design that is suitable for the condition of the pre-elderly oral cavity. Therefore, it is necessary to analyze the design of the number of toothbrush bristles as initial research to obtain an appropriate toothbrush design for the elderly. Method:The study was conducted on 30 participants using a toothbrush with a number of toothbrush bristles paths: with 4 paths, 3 paths, and 2 paths. The effectiveness of tooth brushing hygiene was measured through the debris index before using a toothbrush and after brushing. Result: The results of the Kruskal Wallis test showed a significance value of 0.01 (p

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