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Perubahan Dimensi dan Warna pada Resin Komposit Nanofiller dan Microhybrid Akibat Pemanasan Suhu Tinggi Sebagai Referensi Identifikasi Forensik Rachel Marcelia Hamada; Dwi Kartika Apriyono; Erawati Wulandari
Pustaka Kesehatan Vol 4 No 2 (2016)
Publisher : UPT Percetakan dan Penerbitan Universitas Jember

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Abstract

Backgrounds: Restorative dental material is one of the forensic experts directory assistance to determining the identity of the unknown victim who in burn condition. Determining the identity can be done through changes dimensions. Changes dimensions can be look of reduced weight and discoloration on the restorationat high temperatures. Composite resin restorations is one materials that is mostly used because it has mechanical characteristic, aesthetic and physical well compared to other restorative materials.Objective: To identify the changes ofdimensions such as the weight reduction and the changes ofcolor between nanofiller composite resin with microhybrid composite resin which is heated at high temperatures. Method and Material: The material used is nanofiller composite resin andmicrohybrid composite resin. The stageof thetreatment is done by heating the composite resin at three temperatures: 319oC, 412oC, 800oC then look at the changes ofcolor and the weight reduction.Results and Conclusion: The results of two way ANOVA test and Post Hoc LSD test showed a significant differences with p <0.05. Theconclusion is there are physical changes such as discoloration and weight reductionof the composite resin which is heated at high temperatures.Keywords: Nanofiler composite resin,Microhybrid composite resin,and heated at high temperature.
Hubungan Pengetahuan Kesehatan Gigi dan Mulut dengan Karies Gigi pada Mahasiswa Baru Fakultas Kedokteran Gigi Universitas Jember Tahun Akademik 2016/2017 (Correlation of Oral Health Knowledge with Dental Caries in First Grade Dentistry Students of Jember Galuh Cita Sari Rahtyanti; Hestieyonini Hadnyanawati; Erawati Wulandari
Pustaka Kesehatan Vol 6 No 1 (2018)
Publisher : UPT Percetakan dan Penerbitan Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/pk.v6i1.7153

Abstract

Background: One of major oral health problem found in oral cavity is dental caries. Several factors can induce dental caries such as economical factors, excessive high sugar content foods consumption, and neglect of oral hygiene due to lack of knowledge in how important is teeth and mouth maintenance. Objective: To analyzed correlation between oral health knowledge and dental caries in first grade Dentistry students of Jember University Academic Year 2016/2017. This research used cross sectional approach with total respondents of 119 students. The assessment of oral health knowledge was done by questionnaire and DMF-T index was used to evaluated dental caries in oral cavity. Results and Conclusion: The majority of oral health knowledge distribution in first grade dentistry students of Jember University academic year 2016/2017 was 106 students (89,1%) in good category and DMF-T score of 53 students or 44.5% in very high category.The results of data analyzed by Spearman correlation test could be concluded that there was a significant relation between dental caries and oral health knowledge of first grade Dentistry students Jember University academic year 2016/2017 with moderate correlation strength (-0.460). Keywords : Dental caries, First grade dentistry students, Oral health knowledge
Hubungan antara Pola Sidik Bibir dengan Jenis Kelamin pada Mahasiswa Fakultas Kedokteran Gigi Universitas Jember (The Correlation between Lip Prints Pattern and Sexual Dimorphism on Students of Faculty of Dentistry, The University of Jember) Sitti Nur Qomariah; Masniari Novita; Erawati Wulandari
Pustaka Kesehatan Vol 4 No 2 (2016)
Publisher : UPT Percetakan dan Penerbitan Universitas Jember

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Abstract

Background:Cheiloscopyisaforensicidentificationtechniquesbasedonpatternsofwrinklesandgroovesonthe mucosalsurface ofthelip, calledlipprints. Theunique characteristicsoflipprintsanditsdifferentamonghumanbeingcouldbeusedinidentifypeople.Objective:Theaimofthisstudyistoidentifywhetherthereisacorrelationbetweenlipprintsinsexualdimorphismof studentsofFacultyofDentistry,TheUniversityofJember,ornot.Method:Thedescriptiveobservationalstudyiscrosssectionalapproach.Thesubjectsofthisstudyare80students,comprisingof40malesand40females.Result:Theresultshowedthatthereweredifferenceslipprintspatternbetweenmaleandfemale. Themostdominantlipprint patternin malewastipeIII 16 (40%), whereasthemostdominantlipprintpatterninfemalewastipeI’14(35%).Conclusion:Thedifferencesoflipprintspatternbetweenmaleandfemaleshowedthatlipprintspatterncouldbeused inforensicidentification.Keywords:forensicidentification,lipprintspattern,sexualdimorphism
Analisis Jumlah Sel Monosit yang Mengekspresikan Tnf-Α setelah dipapar Porphyromonas Gingivalis dan Tulang Ikan Kuniran (Upeneus Sulphureus) dengan Teknik Imunositokimia Fiona Budi Amarta Domini; I Dewa Ayu Ratna Dewanti; Erawati Wulandari
Jurnal Kesehatan Gigi Vol 6, No 2 (2019): Desember 2019
Publisher : Jurusan Keperawatan Gigi, Poltekkes Kemenkes Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31983/jkg.v6i2.5480

Abstract

Monocytes play a role in the first defense against microorganisms that harm the body by activating nuclear factor kβ (Nf-kβ) which is the main regulator of inflammatory genes, one of which is TNF-α. TNF-α at excessive levels will cause tissue damage. Therefore an immunomodulator is needed which can regulate the body's immune system. One natural ingredient that acts as an immunomodulator is fish bones. Purpose of research: To analyze the effect of goatfish’s bone extract on TNF-α expression in human monocytes exposed to P. gingivalis. Method: experimental laboratories in vitro. TNF-α expression was tested by imunocytochemical techniques. Results: The results showed that the average number of monocytes expressing TNF-α in group P1 (group of diclofenac sodium) was 42.33 cells per cell. In the P2 group (P. gingivalis group) was 95 cell percent cells and for P3 (fish bone group) was 74.33 cells per cell and the control group was 11.66 cells per hundred cells. Conclusion: goatfish’s bone extract can act as an anti-inflammatory which is effective in reducing TNF-α expression in monocyte exposed to P. gingivalis but less effective than diclofenac sodium.
Cytotoxicity of 5% Tamarindus indica extract and 3% hydrogen peroxide as root canal irrigation Erawati Wulandari
Dental Journal (Majalah Kedokteran Gigi) Vol. 41 No. 3 (2008): September 2008
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (207.53 KB) | DOI: 10.20473/j.djmkg.v41.i3.p107-109

Abstract

Background: Preparation of root canal is an important stage in endodontic treatment. During conducting preparation, it is always be followed with root canal irrigation that has aim to clean root canal from necrotic tissue remains, grind down dentin powder, micro organism, wet the root canal to make preparation process of root canal easier, and solute root canal content at area that can not be reached by equipment. Flesh of Tamarindus indica (pulpa tamarindorum) is used as traditional medicine and it contains vitamin C (antioxidant), protein, fat, glucose, etc. Previous research shows that 5% tamarindus indica extract can clean smear layer but it is more cytotoxicity to cell line BHK–21 than sterilized aquabides. Purpose: This research is to compare cytotoxicity between 5% Tamarindus indica extract with 3% H2O2 as root canal irrigation material. Method: Four teen culture cell line BHK 21 divides into 2 groups. Group 1 is treated with 3% H2O2 and Group 2 is treated with 5% Tamarindus indica extract, for about 2.5 minutes in every group. Then, living and death cell percentage is measured. Data is analyzed with independent t test with significant level of 0.05%. Result: The research showed that death cell in group 1 was 29.3% and in group 2 was 21.1%. There was a significant different (p < 0.05) between group 1 and group 2. Conclusion: Cytotoxicity of 5% Tamarindus indica extract to the cell line BHK–21 is lower than 3% H2O2.
The ability of 5% Tamarindus indica extract as cleaner of the root canal wall smear layer Erawati Wulandari; Latief Mooduto; Theresia Indah Budhy S
Dental Journal (Majalah Kedokteran Gigi) Vol. 40 No. 4 (2007): December 2007
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (261.477 KB) | DOI: 10.20473/j.djmkg.v40.i4.p173-175

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Tamarindus indica is one of traditional medicines. Pulpa tamaridorum consist of organic acid that is usually used as irrigant and to remove root canal wall smear layer. The aimed of this study was to elucidate the ability of 5% Tamarindus indica extract as a root canal irrigant to remove root canal wall smear layer. Eighteen tooth samples were cut on cervical line and divided into 2 then groups were prepared with K file and irrigated. Group 1 was irrigated by sterile aquabidest and group 2 was irrigated by 5% Tamarindus indica extract. Samples were cut longitudinally and formed 7 × 2× 2 mm specimen. Each specimen was photographed by scanning electron microscope, scored and summed. The total score obtained is used as the hygiene value of root canal wall. The collected data were statistically analyzed by using independent t test at 0.05 level. The result of the study showed there was a significant difference between 5% Tamarindus indica extract and sterile aquabidest (p < 0.05), the hygiene value of 5% Tamarindus indica extract was higher than sterile aquabidest. The conclusion of this investigation showed that 5% Tamarindus indica extract remove root canal wall smear layer.
The role of Kuniran (U. moluccensis) and Gurami (O. goramy) fish thorns and scales in increasing salivary leukocyte and monocyte cells viability against Streptococcus mutans I Dewa Ayu Ratna Dewanti; I Dewa Ayu Susilawati; P. Purwanto; Pujiana Endah Lestari; Roedy Budirahardjo; Dyah Setyorini; Ristya Widi Endah Yani; Erawati Wulandari; Melok Aris Wahyukundari
Dental Journal (Majalah Kedokteran Gigi) Vol. 52 No. 1 (2019): March 2019
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1168.806 KB) | DOI: 10.20473/j.djmkg.v52.i1.p45-50

Abstract

Background: Kuniran thorns and Gurami fish scales are rich in protein and minerals such as dentin believed to increase cell viability against Streptococcus mutans (S. mutans) that causes dental caries. These, in turn, can cause systemic diseases if left untreated. Purpose: This study aims to analyze the influence of Kuniran thorns and Gurami fishes scales on the viability of monocytes and salivary leukocytes against S. mutans. Methods: Monocytes and leukocytes salivary cells were placed on a microtiter plate and treated according to the nature of each group. This study comprised the following groups: control group: untreated; S. mutans group: induced by S. Mutans; Gurami thorn group: thorns + S. Mutans; Gurami scales group: scales + S. Mutans; Kuniran thorn group: thorns + S. Mutans; Kuniran scales group: scales + S. mutans. Viability analysis involved staining with Tripan Blue. Furthermore, the number of viable cells (white) was calculated under an inverted microscope at 200 times magnification from five fields of view. Data was analyzed by means of an ANOVA test followed by LSD test. Results: The ANOVA and LSD tests confirmed significant differences (0.01<P). Kuniran thorns and Gurami fish scales increased the viability of monocytes and salivary leukocytes, but not significantly. The content of flavonoids, amino acids, omega 3, omega 6 and antioxidants increased leukocyte metabolism, thereby increasing cell resistance to S. mutans infection. Conclusion: Kuniran thorns (U. moluccensis) and Gurami (O. goramy) fish scales increase the viability of salivary leukocyte and monocyte cells against Streptococcus mutans.
Potential of 5% tamarind extract gel as an etching agent: tensile strength and scanning electron microscope (SEM) evaluation Erawati Wulandari; Faiqatin Cahya Ramadhani; Nadie Fatimatuzzahro
Dental Journal (Majalah Kedokteran Gigi) Vol. 53 No. 1 (2020): March 2020
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/j.djmkg.v53.i1.p16-19

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Background: Acid etching is a stage in obtaining bonds between composites and enamel. The application of acid to the enamel surface, however, can cause dissolution of hydroxyapatite and demineralisation of the enamel surface. Phosphoric acid, a strong acid, is an etching material that can reduce enamel hardness. Excessively reducing hardness can interfere with attachment to the restorative material. One medicinal plant that can be used as an alternative material in acid etching is tamarind. Purpose: This study aims to determine the effect of 5% tamarind extract gel on the tensile strength of composite resins. Methods: This is an experimental research study with a post-test-only control-group design. The study used 14 mandibular incisors. The labial part of the incisor was prepared using a diamond fissure bur with a diameter of 4 mm and a depth of 2 mm. The control group was then etched with 37% phosphoric acid gel, while the experimental group was etched with 5% tamarind extract gel. Bonding resins and micro-hybrid composite resins were applied, based on the manufacturers’ instructions. Next, a tensile strength test and seeing formation resin tags by scanning electron microscope (SEM) were performed. Data were analysed using an independent t-test (p < 0.05). Results: The average tensile strength of composite resins in the group etched with 5% tamarind extract gel was the same as in the 37% phosphoric acid group (p > 0.05). SEM images also show that enamel etched with 5% tamarind extract gel produced a tag similar to that etched with 37% phosphoric acid gel. Conclusion: 5% tamarind extract as an etching material can generate tensile strength of composite resin and trigger formation of resin tags in the same way as 37% phosphoric acid.
Addition of gourami (Osphronemus goramy) fish scale powder on porosity of glass ionomer cement Erawati Wulandari; Farah Rachmah Aulia Wardani; Nadie Fatimattuzahro; I Dewa Ayu Ratna Dewanti
Dental Journal (Majalah Kedokteran Gigi) Vol. 55 No. 1 (2022): March 2022
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/j.djmkg.v55.i1.p33-37

Abstract

Background: Porosity is one of the disadvantages of glass ionomer cement (GIC) restorative materials, as it causes a reduction in strength and durability; the greater the porosity, the lower the strength of the restorative material and vice versa. As gourami fish scales contain calcium and phosphate, they have the potential to reduce the porosity of GIC. Purpose: This study aimed to analyse the effect of adding gourami fish scale powder (GFSP) on the pore size and porosity level of the GIC. Methods: This experimental research included a post-test-only control. The GFSP was fabricated using the freeze-drying method. Sixteen Fuji IX Extra sample cylinders with a diameter of 5 mm and a height of 3 mm were divided into four groups: K0, which comprised GIC without the addition of GFSP; K1, which contained GIC powder + 2.5% GFSP (by weight); K2, which comprised GIC powder + 5% GFSP (by weight), and K3, which contained GIC powder + 10% GFSP (by weight). The samples were observed using scanning electron microscopy and measured using ImageJ software. Data were analysed using a one-way analysis of variance (ANOVA) test. Results: The addition of 2.5% GFSP (by weight) produced the smallest pore size and lowest porosity, while the one-way ANOVA test results were significant among all groups at p = 0.000. There was no significant difference in pore sizes between K0 and K1 (p = 0.359), but a significant difference was found in the level of porosity (p = 0.024). Conclusion: The addition of GFSP affected the porosity of the GIC; the pore size and porosity level of the GIC were reduced by the addition of 2.5% GFSP.
Robusta Coffee (Coffeacanephora) Decreasing IL-1α (Interleukin-1α) Expression and Increasing the Number of Fibroblasts in Healing Process in Dental Pulp in Wistar Rats I Dewa Ayu Ratna Dewanti; I Dewa Ayu Susilawati; Pujiana Endah Lestari; Ristya Widi Endah Lestari; Erawati Wulandari; Roedy Budirahardjo; Dyah Setyorini; Sunlip Wibisono
Journal of Mathematical and Fundamental Sciences Vol. 51 No. 1 (2019)
Publisher : Institute for Research and Community Services (LPPM) ITB

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5614/j.math.fund.sci.2019.51.1.6

Abstract

Fibroblasts and IL-1α are important components of inflammation and healing in dental caries. This healing allegedly could be enhanced by robusta coffee. The aim of this study was to investigate the effect of varying concentrations of robusta coffee on IL-1α expression and the number of fibroblasts. Wistar rats were used, divided into 4 groups and 3 subgroups (7-, 14"‘, and 21-day rats) with each group containing 4 animals: Control group: untreated group; 25% Coffee Group: cavities + treated with 25% coffee bean paste; 50% Coffee Group: cavities + treated with 50% coffee bean paste; 75% Coffee Group: cavities + treated with 75% coffee bean paste. The 7-, 14-, and 21-day rats were sacrificed serially for analysis of the number of dental pulp fibroblasts by staining with HE and IL-1α expression by immunohistochemistry. The resulting data were analyzed descriptively and using ANOVA, followed by an LSD test. The higher the concentration of robusta coffee, the more the expression of IL-1α decreased and the more the number of fibroblasts increased. It is suspected that immunomodulatory proteins inhibit IL-1α expression. It was concluded that robusta coffee could decrease IL-1α expression and increase the number of fibroblasts during the healing process in the dental pulp of Wistar rats.