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Pengaruh Konsentrasi Yeast Saccharomyces Cerevisiae Dan Waktu Fermentasi Terhadap Pembentukan Bioetanol Dari Mikroalga Chlorella sp. Dewi Kusuma Nurmalasari; Sri Rezeki Muria; Chairul Chairul
Jurnal Online Mahasiswa (JOM) Bidang Teknik dan Sains Vol 7 (2020): Edisi 1 Januari s/d Juni 2020
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Teknik dan Sains

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Bioethanol is an alcohol produced from the fermentation process. Bioethanol is also called ethyl alcohol, which is produced through a microbial process, which is the opposite of ethanol synthesized from petrochemical sources. Microalgae Chlorella sp. can be used as raw material for bioethanol by utilizing microorganisms. Microorganisms used to process microalgae Chlorella sp. to become bioethanol is Saccharomyces cerevisiae. The purpose of this study was to determine the effect of Saccharomyces cerevisiae yeast concentration on bioethanol formation and determine the effect of fermentation time on bioethanol formation. This research phase consisted of hydrolysis, inoculum making, bioethanol fermentation, separation and analysis of bioethanol concentration. The variation of bioethanol fermentation time is 1, 2, 3, 4 and 5 days, while the variation of Saccharomyces cerevisiae concentration is 0.2%; 0.4%; 0.6% and 0.8% (w/v). The optimum fermentation results is bioethanol 2% (v/v) or 15.876 g/L. This optimum bioethanol concentration was obtained on the 5th day of bioethanol fermentation with a yeast Saccharomyces cerevisiae concentration of 0.6% (w/v). Keywords: bioethanol, fermentation, microalgae, yeast saccharomyces cerevisiae
Penurunan Kadar COD Pada Limbah Cair Hotel Dengan Sistem Moving Bed Biofilm Reactor (MBBR) Menggunakan Chlorella sp. Mustika Chairani; Shinta Elystia; Sri Rezeki Muria
Jurnal Online Mahasiswa (JOM) Bidang Teknik dan Sains Vol 7 (2020): Edisi 1 Januari s/d Juni 2020
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Teknik dan Sains

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Organic matter contained in the hotel wastewater can be used as a nutrients of microalgae Chlorella sp. so its reduce COD. There are two treatment process happened in MBBR, suspended growth and attached growth by adding biocarriers in the reactor as a place for the propagation of microalgae. This research describes the study of effect of biocarrier (Kaldnes 1 (K1)) filling volume to decreasing COD in hotel wastewater. The study conducted in batches by varying of Kaldnes 1 (K1) filling volume with 4 different levels, that is 0% (without the addition of Kaldnes 1 (K1)), Kaldnes 1 (K1) filling volume as many as 10%, 20%, and 30% (wastewater volume). This process takes up to 5 days with solar irradiation in the MBBR. The result showed that 20% Kaldnes 1 (K1) filling volume happened in days-5 was able to reduce COD with efficiency of COD is 81.82%.Keyword: Chlorella sp., MBBR, Hotel Wastewater, COD
Pengaruh Laju Alir Terhadap Substrat Pada Fermentasi Reject Nanas Menjadi Bioetanol Secara Kontinu Okky Rizky Sinaga; Adrianto Ahmad; Sri Rezeki Muria
Jurnal Online Mahasiswa (JOM) Bidang Teknik dan Sains Vol 2, No 2 (2015): Wisuda Oktober Tahun 2015
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Teknik dan Sains

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Today, the world's need for energy is increasing while the supply of energy from fossil fuels that have been relied upon a limited number. Therefore, it is necessary that alternative energy sources are able to overcome the energy crisis. One of the alternative energy sources are being developed is bioethanol. Bioethanol can be produced by fermentation of glucose using the yeast Saccharomyces cerevisiae. One of source of glucose is pineapple. This research aims to make bioethanol from pineapple fruit skin using continuous fermentation method with a flow rate variations. Fixed variable used is the 5-liter, pH 4.5, inoculum levels of 0.3%, 0.5% urea, phosphorus levels and temperature fermentation 0.08% used is room temperature. Variable changes used are changes in feed flow rate is 5 L / day; 2.5 L / day; 1.67 L / day; 1:25 L / day and 1L / day. Experimental procedure includes pretreatment, inoculation, fermentation and purification using a vacuum evaporator. Analysis performed is analyzing glucose and ethanol concentration analysis. The results were obtained concentration of ethanol in the steady state condition for the flow rate of 5L / day as much as 5% v / v; 2.5 L / day by 7% v / v; 1.67 L / day as much as 6.5% v / v; 1.25 L / day as much as 6% v / v; and 1 L / day as much as 4% v / v. The highest ethanol concentration was obtained at a flow rate of 2.5L / day as much as 7%. This study suggests that the concentration of ethanol fermentation results increases with the flow rate of the substrate to achieve the optimal flow rate then decreased after reaching the optimum flow rate.Keywords: bioethanol, continuous, pineapple skin, saccharomyces cerevisiae
Isotherm Dan Kinetika Adsorpsi Logam CU Menggunakan Adsorben Serbuk Kulit Udang Dengan Variasi Konsentrasi Adsorbat Dan pH Larutan M. Rafi Yunanda; Yelmida A; Sri Rezeki Muria
Jurnal Online Mahasiswa (JOM) Bidang Teknik dan Sains Vol 7 (2020): Edisi 2 Juli s/d Desember 2020
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Teknik dan Sains

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Adsorption is a process in which solute liquids accumulate on the adsorbent surface and form a thin layer of atoms or molecules. The adsorbent used was shrimp shell powder. Utilization of the adsorptionprocess in the absorption of Cu metal is useful for determining the isotherm model and adsorption kinetics. In this study the adsorption process was carried out by varying the concentration of adsorbate(10, 20 and 30 ppm) and the pH of the Cu metal solution (5, 7 and 9) at room temperature. The largest adsorption capacity occurred at the adsorption pH of 5 and the initial solution concentration of 10 ppmwith an adsorption capacity value of 88.28%. The isotherm model of the Cu metal adsorption process follows the Harkins Jura isotherm equation with R2 value of 0.9990. The kinetics of the Cu adsorptionprocess followed the Elovich kinetics model with a value of R2 0.9914 and a constant adsorption rate of 0.631 at an adsorbate concentration of 10 ppm and a pH of 7 Cu metal solution.Keywords : Adsorbent, Adsorption, Isotherm, Kinetics, pH
Pengaruh Perbandingan Eceng Gondok Dan Air Terhadap Penyisihan COD (Chemical Oxygen Demand) Dan Padatan Pada Produksi Biogas tahap Metanogenesis Nurul Annisa; Sri Rezeki Muria; Adrianto Ahmad
Jurnal Online Mahasiswa (JOM) Bidang Teknik dan Sains Vol 2, No 2 (2015): Wisuda Oktober Tahun 2015
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Teknik dan Sains

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Biomass of water hyacinth (Eicchornia crassipes) is a weed plant that is growing very fast. Components of water hyacinth has a high cellulose that potential to be used as raw material for production of biogas by anaerobic fermentation. The purpose of this study to determine the effect of substrate ratio for Chemical Oxygen Demand (COD) removal and solids removal and the maximum process conditions. This process using a 15 liters of batch bioreactor at pH 7 with variation of water hyacinth and water 1: 2, 1: 3, 1: 4 and 1: 5. Observations biogas production is done by measuring the volume of biogas produced each day, while organic matter and solids content by seeking removal efficiency of COD and solids removal. The results showed that COD removal efficiency of the largest in the ratio of 1:2 is 80,85% and the total solids removal efficiency at a ratio of 1:2 was 63.04%, 1:3 was 54.49%, 1:4 was 36.32%, and 1:5 was 31.25%. The largest biogas production substrate ratio of 1: 2 at 23,300 mL and the results of methane analysis using gas chromatography amounted to 11924.56 ppm (1,19%).Keywords: anaerobic, batch, biogas, gas chromatography, water hyacinth
Pengaruh Laju Aerasi Terhadap Produksi Kitinase Oleh Trichoderma Asperellum TNJ63 Riko Naldo Saputra; Sri Helianty; Sri Rezeki Muria
Jurnal Online Mahasiswa (JOM) Bidang Teknik dan Sains Vol 4, No 2 (2017): Wisuda Oktober Tahun 2017
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Chitinase is enzyme that hydrolyze chitin compound in β-1,4-N-acetyl-glucosamine into monomer N-acetyl-glucosamine, which widely occurs in nature. This enzyme is potentially applicable in the field of pharmaceuticals for the treatment of cancer, tuberculosis, asthma, diabetes, allergies and infections. Chitinase can be produced by microorganisms chitinolytic are numerous in the biosphere, one of the local isolates Trichoderma asperellum TNJ63. In this research, scale up production of 25 mL to 1.5 L medium. Fermentation was carried out in a 2 L stirred tank bioreactor using methods submerged fermentation (SmF) with the substrate chitin from shrimp shells. Chitinase production conducted for 9 days with stirring speed of 40-60 rpm, and the effect of aeration rates (0 vvm; 0.5 vvm; 1 vvm and 1.5 vvm) studied the chitinase activity produced. The highest chitinase activity was obtained at 1 vvm aeration rate and fermentation time of 7 days with 0.0160 U / mL through analysis UV-Vis spectrophotometer with a sugar analysis method, Nelson-Somogyi.Keywords: aeration, chitinase, scale up, shrimp shells, Trichoderma asperellum TNJ63
Pengaruh Pemberian Limbah Cair Tahu Dan Fotoperiod Terhadap Kadar Glukosa Pada Kultivasi Mikroalga Scenedesmus sp. Dian Larasati; Shinta Elystia; Sri Rezeki Muria
Jurnal Online Mahasiswa (JOM) Bidang Teknik dan Sains Vol 6 (2019): Edisi 1 Januari s/d Juni 2019
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More energy needs are met from fossil energy, but that fuel can’t be renewed. Various studies have been conducted to utilize microalgae as raw material for biofuels. The carbohydrate content in microalgae can be used as raw material for bioethanol. Scenedesmus sp. considered a promising microalgae for biofuel production because it has a high level of growth and glucose production, and can utilize organic matter as a nutrient in the form of tofu liquid waste so that the synergy between wastewater treatment and biomass production can run well. The purpose of this study was to determine the optimal conditions for growth of Scenedesmus sp. with the influence of photoperiod and volume of tofu wastewater on glucose levels formed after the cultivation process. In this study variations in the provision of tofu wastewater in the cultivation medium (0%, 20%, 40%, 60%, 80%, 100%) with the ratio of photoperiod light:dark (16:8, 14:10, 12:12, and 24:0) and the time of data collection on days are 0, 1, 3, 5, 7, 9, 11, and 13. Based on the results of the study obtained the highest glucose level of 36,41 mg/l at the added of 20% tofu liquid waste and photoperiod 12:12. Keywords: Glucose, tofu liquid waste, photoperiod, Scenedesmus sp.
Pengaruh Kecepatan Putaran Pada Proses Rotary Alga Biofilm Reactor (RABR) Untuk Penyisihan COD Menggunakan Mikroalga Chlorella sp. Pada Limbah Cair Domestik Mutia Nurrahmadhani; Shinta Elystia; Sri Rezeki Muria
Jurnal Online Mahasiswa (JOM) Bidang Teknik dan Sains Vol 7 (2020): Edisi 2 Juli s/d Desember 2020
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Teknik dan Sains

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Domestic liquid waste has organic matter and high pollutant load. Microalgae Chlorella sp. is able to utilize organic material as a source of nutrition for its growth, hence it may reduce pollutant of domestic liquid waste. Microalgae Chlorella sp. which grows on the surface of the disk is suspended in domestic liquid waste using a Rotary Alga Biofilm Reactor (RABR) is able to absorb high level nutrient, so that they have high cell growth. In this research, it is conducted by using speed rational variations in RABR process (2, 4, dan 6 rpm) and contact time (0, 1, 3, 5, 7 days) to remove COD parameters. Based on the results toward the removal of pollutant parameters by using 4 rpm rotational speed variation is higher than using 2 and 6 rpm. The concentration of COD removal of 210 mg/L with the removal efficiency of 73,08 %.Keywords: Chlorella sp, COD, Rotation Speed, Domestic Liquid Waste, Rotary Alga Biofilm Reactor (RABR)
Peningkatan Kandungan Glukosa Mikroalga Scenedesmus SP. Yang Dikultur Pada Variasi Limbah Cair Tahu Dan Intensitas Cahaya Yang Berbeda Aisyah Sri Lestari; Shinta Elystia; Sri Rezeki Muria
Jurnal Online Mahasiswa (JOM) Bidang Teknik dan Sains Vol 6 (2019): Edisi 1 Januari s/d Juni 2019
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Teknik dan Sains

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Energy use still depends on petroleum energy which is non-renewable energy. As a solution, it is necessary to diversify energy other than petroleum. One of energy diversification is by utilizing microalgae as bioenergy raw material. Scenedesmus sp. is one of the microalgae which has the potential as a bioenergy feedstock. This study aims to determine optimal conditions of growth microalgae Scenedesmus sp. with the effect of the volume of tofu wastewater and different light intensities on glucose. In this research, the observation with a ratio the volume of bold basal medium and tofu liquid waste 0%, 20%, 40%, 60%, 80% and 100% and light intensity of 1000 lux, 2000 lux, 3000 lux and 4000 lux. Based on the research results obtained optimal conditions on the volume of tofu liquid waste 20% and light intensity 4000 lux with glucose 41.9 mg/L.Keywords : Scenedesmus sp., glucose, tofu liquid waste, light intensity.
Pengaruh Kecepatan Pengadukan Pada Sistem High Rate Algae Reactor Terhadap Spesific Growth Rate Mikroalga Dan Mixed Liquor Suspended Solid Dalam Palm Oil Mill Efluent Fauzy Isnandar; Shinta Elystia; Sri Rezeki Muria
Jurnal Online Mahasiswa (JOM) Bidang Teknik dan Sains Vol 7 (2020): Edisi 1 Januari s/d Juni 2020
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Teknik dan Sains

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The stabilization pond system are used in almost all of palm oil mills in Indonesia. One of it disadvantages is that it disable to exploit the potential of microalgae. HRAR is a technology that is able to increase microalgae biomass, and with a consortium of microalgae-bacteria, the elimination of organic matter is increasing. This research aim by determined the effect of mixing velocity in the HRAR system towards the spesific growth rate of microalgae and mixed liquor suspended solid in POME. The research was carried out in batches for 7 days by using solar energy as a source of light. Mixing were operated 24 hours per day with by variations of mixing velocity was 16, 20, and 24 cm/sec. Based on the research results, the mixing velocity of 20 cm/sec was the best velocity for indigenous microalgae. The highest spesific growth rate of microalgae was 0,402/day and mixed liquor suspended solid values was 3625 mg/L .Keywords: High Rate Algae Reactor (HRAR), Mixing, Microalgae, Palm Oil Mill Effluents (POME).
Co-Authors Adella, Lufya Adli Satria Sandika Adrianto Ahmad Adrianto Ahmad Aisyah Sri Lestari Andhika Chandra Artha, Ulfa Dwi Aulia Masta Bahruddin Bahruddin Bahruddin Bonita Esther FS Boy Jansen Roberto Manik Chairul Chairul Chairul Chairul Darmayanti, Indah David Andrio Devita Ulfa Sari, Devita Ulfa Dewi Kusuma Nurmalasari Dian Larasati Dian Larasati Diana Aprila Dina Citra Naomi Dina Citra Naomi Harianja Doni Fozla Dwi Laura Pramita Elfina Noviarni Chandra Elgina May Lestari Elvi Yenie, Elvi Erlangga, Herta Furaida EVELYN EVELYN Evelyn Evelyn Fajril Akbar Fanani, Adita Suri Fauzani, Rhanti Fauzy Isnandar Febrina Adelina Sigalingging Fikri Miftahul Shiddiq Firmanto, Firmanto fitriyanti fitriyanti Gita Aziza Salis, Gita Aziza Hamzah, Nirwana Hasti, Fucy Adilla Hasti, Fucy Adilla Hengky Darma Satria, Hengky Darma Hilmiyati Hilmiyati Ida Zahrina Ikhsan ' Fajrin Indah Darmayanti Darmayanti Indah Kartika Zulfa Indah Kartika Zulfa Indra Purnama Insani, Suciati Dwi Irdoni HS Irma Damayanti Isabella Amalia Denisa Putri Jeckson, Edie Jelizanur Jelizanur Jessar Hendrianto Juarsa, Tri Khairat Komalasari Komalasari Larasati, Dian Lely Rahmawati Saragih Lidya Anggraini Lita Darmayanti M. Rafi Yunanda Masriza, Maya Masroah Tuljannah Mastiar Krisdayanti Sinaga Maya Ratna Sari Mayang Sari Meilano Ashari Akbar Meisy Dhyta Amelia Meisy Dhyta Amelia Meliana Dewi Mesy Susri Darsi Muhammad Rivai Mustika Chairani Mustika Chairani Mutia Nurrahmadhani Niken Triani Klaway Reza Nova Diyana Nurhanifah Nova Ramadhani Farnas Novebriantika Novebriantika Novira, Tria Bela Novriandi, Rizky Nur Irfana Mardiyah Nurul Annisa Nuryanti, Lidya Oding Sitorus Okky Rizky Sinaga Padil Padil Purwoko, Agus Putri, Aulia Dewi Putri, Bella Nadiah Rahani Rahani Rendy Hidayat Resna Rauda Pratiwi Resna Rauda Putri Resti Ossi P Riko Naldo Saputra Rizkya Ramadhani Rohaya, Rohaya Roin Nuretha Roy Ronald Siburian, Roy Ronald Rozanna Sri Irianty Sakinah Rhajani Sally Mandari Sandy Juliana Sari Santoso Nugroho Sari, Nurriya Mayang Sheilviana Angela Shindi Unada Shinta Elystia Silvia Reni Yenti Sitinjak, Jufri Suanto Sri Armita Sari Sri Helianty Sri Indira Puspa Pertiwi Sri Indira Puspa Pertiwi SUSANTO, RENO Syelvia Putri Utami Tantri Wilinda Julia Tasbieh, Hayatrie Tri Lusi Lisa Dila Vonny Meidina Rizani Vonny Meidina Rizani Wenny Andini Wulandari, Zarah Ayu Yelmida Azis Yolanda Amalia Yuliyana Rahmawati Zahra Nadia Elysahaq Zuchra Helwani Zul Fadly Khaira Zuqni Meldha