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All Journal Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Biogenesis: Jurnal Ilmiah Biologi Jurnal Sumberdaya Alam dan Lingkungan Bioma : Jurnal Biologi dan Pembelajaran Biologi Indonesian Journal of Chemical Analysis (IJCA) Mitra Mahajana: Jurnal Pengabdian Masyarakat Sriwijaya Bioscientia AMMA : Jurnal Pengabdian Masyarakat
Achmad Arifiyanto
Department Of Biology, FMIPA Universitas Lampung
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Decision Sciences, Operations Research & Management Earth & Planetary Sciences Education Environmental Science Immunology & microbiology Industrial & Manufacturing Engineering Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Medicine & Pharmacology Nursing Public Health Social Sciences

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Bioakumulasi dan Aktivitas Resistensi Logam Timbal (Pb) terhadap Streptomyces sp. strain I18 Mutia Dinda Lestari; Mesy Miranda AR; Ulin Ni'mah Setiawati; Nismah Nukmal; Endah Setyaningrum; Achmad Arifiyanto; Titik Nur Aeny
Jurnal Sumberdaya Alam dan Lingkungan Vol 9, No 1 (2022)
Publisher : Brawijaya University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jsal.2022.009.01.1

Abstract

ABSTRAKKegiatan antropogenik secara intensif mengakibatkan pencemaran lingkungan yang disebabkan oleh logam berat. Timbal (Pb) merupakan salah satu logam berat toksik. Tingkat toksisitas dapat diturunkan melalui proses bioakumulasi oleh mikroorganisme. Penelitian ini dilakukan untuk mengetahui kemampuan bakteri Streptomyves sp. strain I18 dalam mengakumulasi logam Pb. Bakteri diidentifikasi morfologi secara makroskopik dan mikroskopik. Daya resistensi bakteri terhadap logam Pb ditentukan dengan menumbuhkan bakteri pada media Muller-Hilton Agar yang disuplementasi Pb pada konsentrasi 5, 50, dan 150 ppm menggunakan metode dilusi pada cawan petri petri. Bioakumulasi bakteri terhadap logam Pb diukur menggunakan Atomic Absorption Spectroscopy (AAS) pada konsentrasi 5, 50, dan 150 ppm. Bakteri Streptomyves sp. strain I18 mampu tumbuh pada media padat agar mengandung logam Pb pada bagai konsentrasi dengan diameter koloni terbesar, yakni 50.33 mm pada konsentrasi Pb 5 ppm. Pertumbuhan koloni terkecil sebesar 33.00 mm terjadi pada konsentrasi 150 ppm. Bakteri ini terbukti mampu mengakumulasi logam Pb dengan akumulasi terbesar pada konsentrasi 5 ppm, sebesar 49%. Peningkatan konsentrasi logam menurunkan kemampuan akumulasi logam Pb. Pada konsentrasi 150 ppm dan 50 ppm, bakteri hanya mampu mengakumulasi Pb sebesar 5% dan 9%. Kemampuan optimal bakteri Streptomyves sp. strain I18 sebagai bioakumulator diperkirakan pada konsentrasi kurang dari 50 ppm.Kata kunci: bioakumulasi, Pb, resistensi, Streptomyces ABSTRACTAntropogenic activity intensively can cause environmental pollution by heavy metals. Lead is one of toxic heavy metal. Level of toxicity can be decreased with bioaccumulation by microorganisms. This research conducted to know the ability of bacteria Streptomyces sp. strain I18 to accumulate lead. The morphological of bacteria was identified by microscopic and macroscopic. Bacterial resistance of lead has been known by growing bacterial in Muller-Hilton Agar supplemented lead in concentration 5, 50, and 150 ppm uses dilution plate method. Biaccumulation of bacteria against lead measured by Atomic Absorption Spectroscopy (AAS). Streptomyces sp. strain I18 can grow in solid agar media contains lead in various concentration. The highest diameter of colony 50.33 mm measured in media combined lead concentration 5 ppm. The lowest growth of colony is 33.00 mm in lead concentration of 1000 ppm. These bacteria are proven that can accumulate lead with the highest accumulation reached 49% in concentration of 5 ppm. The concentration of 150 and 50 ppm can accumulate lead reached 5% and 9%. Optimal ability of bacteria Streptomyces sp. strain I18 as bioaccumulator supposed in concentration of less than 50 ppm.Keywords: bioaccumulation, lead, resistance, Streptomyces
Wawasan Perilaku Hidup Bersih dan Sehat (PHBS) di Masa Kenormalan Baru dalam Upaya Mencegah Penyebaran Covid-19 di Lingkungan Sekolah Rizal Koen Asharo; Achmad Arifiyanto; Firas Khaleyla; Choirina Tamimi Rahmadi
Mitra Mahajana: Jurnal Pengabdian Masyarakat Vol. 2 No. 2 (2021): Volume 2 Nomor 2 Tahun 2021
Publisher : LPPM Universitas Flores

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37478/mahajana.v2i2.977

Abstract

The Indonesian government has long supported educational institutions to provide clean and healthy living habits through the Ministry of Health. However, clean and healthy living habits in schools seem to be increasingly important nowadays, because in the new normal era of the corona virus pandemic, children will soon return to school. Schools must maintain a clean and healthy lifestyle by involving teachers, students, and the community in the school environment and following certain health-related procedures. The school environment also needs to be made clean and healthy. The benefit of maintaining a clean and healthy lifestyle at school is to create a clean and healthy environment. In this way, the teaching process will run smoothly, and the health of teachers, students and the surrounding community will not be disturbed. Education on clean and healthy living habits is packaged in a national seminar in collaboration with SMA Negeri 19 Surabaya on Sunday, March 28, 2021. This activity is carried out in the form of lectures with the help of power points, through a zoom meeting platform which is also broadcast live on Youtube. The material presented includes three things with the author himself as the resource person and moderator of the event. The three materials are about Covid-19 and its development, the Covid-19 vaccine and its effectiveness, and the application of clean and healthy living habits in an effort to fight Covid-19. As for the participants, students from SD, SMP, and SMA with a total of 78 people and general participants reaching 11 people. This service is carried out independently and is carried out well in accordance with the objectives expected by the executor.
Aktivitas Enzim Hidrolase pada Penapisan Isolat Actinomycetes Kandidat Probiotik Udang Sumardi Sumardi; Vanya Qatrunada; Salman Farisi; Achmad Arifiyanto; Christina Nugroho Ekowati
BIOMA Vol 6, No 1 (2021): BIOMA:JURNAL BIOLOGI DAN PEMBELAJARAN BIOLOGI
Publisher : Universitas Muhammadiyah Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32528/bioma.v6i1.3548

Abstract

Actinomycetesis one of the microorganisms which have the ability of enzymes hydrolases such as cellulose, amylase, protease, and manannase. Manufacturer of enzymes of the hydrolase can be used as a candidate probiotic. A candidate probiotic should have a good resistance in different environmental conditions including pH and salinity.  This research is conducted to filter the candidate’s probiotics from bacteria Actinomycetes that tolerance salinity and has the activity of the enzyme hydrolase. Testing the activity of the enzyme was preformed qualitatively on the variation of pH 4, 7, and 9.8.  Theability of Atinomycetesproduce enzymes hydrolases is indicated by the colony on media containing the substrate.From the test results, cellulase enzyme activity with the largest enzymatic index value is 3.5 AF2 isolates pH 9.8. Amylase enzyme activity with the largest enzymatic index value is 3.66 RH1 isolates pH 7. Protease enzyme activity with the largest enzyme index value is 0.66, AF2 isolates at pH 7. Mananase enzyme activity with the largest enzymatic index value is 0.85, namely AF2 isolates pH 4. In testing the effect of salinity test isolates were able to grow at variations in the concentration of NaCl 0, 3, and 6%.
Aktivitas Biosurfaktan Serratia Marcescens strain MBC1 dalam Mengemulsikan Solar dengan Variasi pH dan Media Cindy Lukyta Ratih Riyanto; Sumardi Sumardi; Salman Farisi; Christina Nugroho Ekowati; Achmad Arifiyanto
Jurnal Sumberdaya Alam dan Lingkungan Vol 8, No 3 (2021)
Publisher : Brawijaya University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jsal.2021.008.03.3

Abstract

ABSTRAK Resiko pencemaran lingkungan akibat tumpahan solar meningkat tiap tahunnya, oleh karena itu diperlukan upaya ramah lingkungan dengan biaya produksi rendah. Penelitian ini menggunakan bakteri Serratia marcescens strain MBC1 dengan tujuan menguji aktivitas biosurfaktan yang dihasilkan dalam melarutkan solar. Bakteri ini ditumbuhkan pada media produksi Trypton Water, limbah cair produksi tepung tapioka, dan limbah cair produksi  tepung maizena yang masing-masing telah diberi variasi pH yaitu 6, 7, dan 8 kemudian diinkubasi selama 7 hari. Biosurfaktan dari media produksi dipanen dengan sentrifuse dan diuji aktivitas biosurfaktan dengan 3 parameter uji yaitu uji drop collapse, uji oil displacement, dan uji emulsifikasi. Hasil penelitian menunjukkan bahwa biosurfaktan yang dihasilkan dari ketiga jenis media produksi mampu melarutkan solar. Biosurfaktan hasil produksi dari media limbah cair produksi tepung maizena pH 7 menunjukkan aktivitas melarutkan solar paling optimum dengan indeks emulsifikasi sebesar 63.88%.Kata kunci:  biosurfaktan, Serratia marcescens strain MBC1, solarABSTRACTThe risk of environmentall pollution due to diesel fuel spills increases every year therefore it takes an effort that is safe for the environment with low production costs. These bacteria are grown in the media productions that are Tryptone Water, cornstarch wastewater and tapioca wastewater each of them had been given pH variation that are 6, 7, and 8 then incubated for 7 days. Biosurfactants from media productions were harvested by centrifuge and tested for biosurfactant activity with 3 test parameter there are drop collapse test, oil displacement test, and emulsification test. The result showed that the biosurfactants produced from the three types of media production were able to dissolve diesel fuel. Biosurfactant produced from cornstarch wastewater pH 7 showed the most optimum dissolving of diesel fuel with emulsification index is 63.88%.Keywords:  biosurfactant, Serratia marcescens strain MBC1, diesel fuel
Analisis Senyawa Bioaktif Ekstrak Metabolit Sekunder Serratia marcescens strain MBC1 Yusifa Arsy Variani; Endah Setyaningrum; Kusuma Handayani; Nismah Nukmal; Achmad Arifiyanto
Indonesian Journal of Chemical Analysis (IJCA) Vol. 4 No. 2 (2021): Indonesian Journal of Chemical Analysis
Publisher : Universitas Islam Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20885/ijca.vol4.iss2.art3

Abstract

Serratia marcescens strain MBC1 merupakan bakteri gram negatif yang dapat menghasilkan beberapa senyawa bioaktif. Berdasarkan penelitian sebelumnya, diketahui bahwa bakteri ini mampu mendegradasi berbagai macam enzim dan memiliki berbagai macam aktivitas biologis seperti antibakteri, antikanker, biosurfaktan dan sebagai bahan obat-obatan. Tujuan penelitian ini adalah untuk mengetahui kandungan senyawa bioaktif yang terdapat pada ekstrak metabolit sekunder Serratia marcescens strain MBC1. Pada penelitian ini dilakukan beberapa uji yaitu, uji fitokomia dan uji FT-IR. Hasil uji fitokimia menunjukkan bahwa ekstrak Serratia marcescens strain MBC1 mengandung senyawa golongan alkaloid dan saponin. Hasil uji FT-IR menunjukkan adanya kemiripan gugus fungsi yang dimiliki oleh ekstrak S.marcescens strain MBC1 dengan senyawa golongan alkaloid. Namun, perlu adanya penelitian lebih lanjut mengenai kandungan senyawa metabolit yang terkandung dalam ekstrak S. marcescens dan aktivitas biologisnya sebagai antimalaria, antibakteri, antifungi dan sebagai bahan obat-obatan. 
Pengaruh Media Pertumbuhan dan pH Terhadap Aktivitas Biosurfaktan dari Bakteri Serratia marcescens strain MBC 1 pada Minyak Jelantah Berliana Damayanti; Sumardi Sumardi; Achmad Arifiyanto; Kusuma Handayani; M. Kanedi; Meishy Handerlin Putri; Cindy Lukyta Ratih Riyanto
Indonesian Journal of Chemical Analysis (IJCA) Vol. 5 No. 1 (2022): Indonesian Journal of Chemical Analysis
Publisher : Universitas Islam Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20885/ijca.vol5.iss1.art1

Abstract

Campuran minyak dan bahan kimia pada surfaktan yang dibuang langsung ke lingkungan akan mengakibatkan penurunan kesuburan tanah sertamenghambat proses degradasi oleh mikroorganisme. Oleh karena itu dibutuhkan senyawa alami yang mampu mampu meningkatkan kelarutan minyak jelantah dalam air seperti biosurfaktan. Salah satu bakteri penghasil biosurfaktan yaitu Serratia marcescens. Penelitian ini bertujuan untuk mengetahui aktivitas biosurfaktan bakteri dari Serratia marcescens strain MBC 1 yang ditumbuhkan di media fermentasi tryptone water, limbah cair jagung dan limbah cair singkong dengan pH 6,7 dan 8. Uji yang dilakukan diantaranya uji emulsifikasi, oil displacement dan drop collapse. Hasil penelitian menunjukan biosurfaktan Serratia marcescens strain MBC 1 mampu meningkatkan kelarutan minyak jelantah dalam air. Hasil produksi pada media limbah jagung dengan pH 7 menunjukan aktivitas emulsifikasi paling optimum yaitu sebesar 49,26%.
Kajian daya hambat ekstrak antibakteri dari isolat Actinomycetes terhadap Vibrio sp. resisten Ampisilin dan Tetrasiklin Ratna Claudya Naomi Hutagalung; Christina Nugroho Ekowati; Salman Farisi; Sumardi Sumardi; Achmad Arifiyanto
Sriwijaya Bioscientia Vol 2 No 1 (2021)
Publisher : Biology Department, Faculty of Mathematics and Natural Sciences, Sriwijaya University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1383.688 KB) | DOI: 10.24233/sribios.2.1.2021.184

Abstract

Actinomycetes dengan kode isolat RH dan AF diisolasi dari tanah mangrove Hanura, Lampung Timur dan tanah rizosfer Sidoarjo. Kedua isolat diidentifikasi secara mikroskopis dan makroskopis. Isolat diseleksi berdasarkan aktivitas antibakteri, uji tantang terhadap Vibrio sp., dan didukung dengan uji patogenitas untuk mengetahui sifat patogen dari isolat tersebut.  Zat antibakteri dari isolat diperoleh melalui ekstraksi dengan pelarut metanol dan etil asetat.  Hasil penelitian menunjukkan bahwa isolat RH dan AF mampu menghambat pertumbuhan Vibrio sp. resisten antibiotik. Pada uji tantang terhadap Vibrio sp. resisten antibiotik terbentuk zona jernih diantara kedua isolat yang menunjukkan bahwa isolat RH memiliki kemampuan untuk menghambat pertumbuhan Vibrio sp. resisten antibiotik. Pada uji patogenitas kedua isolat Actinomycetes RH tidak menunjukan kemampuan hemolisis apapun yang dibuktikan dengan tidak terbentuknya zona jernih pada medium agar darah, namun pada isolat AF menunjukan kemampuan β-hemolisis yang dibuktikan dengan terbentuknya zona jernih pada medium agar darah.
Penapisan Enzim Hidrolase pada Bakteri Streptomyces sp. strain I18 Ulin Ni'mah Setiawati; Mesy Miranda AR; Mutia Dinda Lestari; Nismah Nukmal; Endah Setyaningrum; Titik Nur Aeny; Achmad Arifiyanto
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 7, No 3 (2022): October 2022
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v7i3.5179

Abstract

Enzim hidrolase memiliki peranan penting dalam proses industri salah satunya sebagai biokatalisator. Bakteri sebagai produsen enzim hidrolase dapat dimanfaatkan potensinya dalam proses industri. Penelitian ini bertujuan untuk mengetahui potensi enzim hidrolase dari Streptomyces sp. strain I18 koleksi Laboratorium Mikrobiologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Lampung. Pengujian dilakukan secara kualitatif dengan mengukur zona bening yang terbentuk disekitar isolat. Diameter zona bening diukur dan dibagi dengan diameter koloni isolat yang tumbuh untuk mendapatkan nilai indeks enzimatis. Zona jernih merupakan indikator adanya aktivitas enzimatik pada mikroorganisme tersebut.  Pada penelitian ini didapatkan bahwa Streptomyces sp. strain I18 memiliki potensi yang tinggi dalam mendegradasi pati dengan enzim amilase yang dihasilkannya dengan nilai indeks enzimatis yang diperoleh sebesar 2,94. Selain itu juga memiliki potensi menghasilkan enzim protease, kitinase, mananase, selulase dan lipase dengan nilai indeks enzimatisnya secara berurutan, yaitu 1,89, 0,25, 0,36, 1,00, dan 2,26. Kesimpulannya, enzim hidrolase yang dihasilkan oleh Streptomyces sp. strain I18 memiliki potensi untuk digunakan sebagai kandidat biokatalisator di berbagai bidang, seperti industri, biomedis, dan agen biokontrol. 
Screening of the bio-decolorization ability of synthetic dyes and the degradation of hydrocarbon bacteria Serratia marcescens MBC1 Achmad Arifiyanto; Berliana Damayanti; Meishy Handerlin Putri; Cindy Lukita Ratih Riyanto
Biogenesis: Jurnal Ilmiah Biologi Vol 10 No 2 (2022)
Publisher : Department of Biology, Faculty of Sci and Tech, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v10i2.32095

Abstract

Humans are inseparable from using hydrocarbons, including fossil fuels, dyes, and oleochemical products. This has frequently resulted in a high possibility of waste spills polluting the environment. A biological approach using microbes was used to facilitate many of these countermeasures. This study aimed to determine the ability of the bacterium Serratia marcescens MBC 1 isolate as a biodegradation agent for various hydrocarbons and a bio-decolorizing agent for synthetic dyes. The bacterial growth media used were solid and tryptic soy broth. Methylene blue, congo red, methyl red, methyl orange, and crystal violet were synthetic dyes at a concentration per million. Within 7 days, several synthetic dyes tested showed decolorization. Methylene blue has the fastest decolorization time, taking just 24 h. The lipase index method was used to assess the propensity of hydrocarbons to degrade qualitatively. Kerosene had the highest lipolytic index at 6.31, followed by used cooking oil at 5.48 index, used lubricant at 5.37 and diesel at 3.63 index. Quantitative and comprehensive in-depth testing of the potential achievements of this initial test will be used in solving environmental problems that may occur further, especially related to the impact of the use of synthetic dyes.
Production of Resistant Starch from Avocado Seeds (Persea americana) through Streptomyces sp. AB8 Fermentation and Autoclaving-Cooling Variations Achmad Arifiyanto; Siti Inah; Indah Sukma Ningsih; Christina Nugroho Ekowati; Tundjung Tripeni Handayani
Indonesian Journal of Chemical Analysis (IJCA) Vol. 6 No. 1 (2023): Indonesian Journal of Chemical Analysis
Publisher : Universitas Islam Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20885/ijca.vol6.iss1.art3

Abstract

Agricultural waste with a high starch content potentially serves as a source of resistant starch. The avocado seed has not been explored much and is often thrown away as waste in consuming avocados. Resistant starch has properties and functions as dietary fiber and has advantages as a prebiotic. This study aims to determine the effect of fermentation treatment using Streptomyces sp. AB 8 and variations in the number of pressure-cooling heating cycles to increase avocado seed-resistant starch. The research phase consisted of pre-treating the avocado seeds and fermentation by Streptomyces sp. AB 8 followed by a pressure heating-cooling stage with 0, 1, 2, and 3 cycles. Concerning modified starch, this study also measured levels of starch, amylose, amylopectin, and resistant starch. Digestion test using Lactobacillus sp. by viewing the clear zone around the colonies after 48 hours to evaluate the digestibility of resistant starch. The results of this study indicate that the fermentation treatment by Streptomyces sp. AB 8 and pressure-cooling heating can increase the levels of resistant starch in avocado seeds. Fermentation treatment with two cycles of heating-pressure-cooling (S1) produced the best resistant starch content of 7.51% and had the highest enzymatic index of 2.48 ± 0.45.
Co-Authors Berliana Damayanti Berliana Damayanti Choirina Tamimi Rahmadi Christina Nugroho Ekowati Christina Nugroho Ekowati Christina Nugroho Ekowati Christina Nugroho Ekowati Cindy Lukita Ratih Riyanto Cindy Lukyta Ratih Riyanto Cindy Lukyta Ratih Riyanto Dzul Fithria Mumtazah Emantis Rosa Endah Setyaningrum Endah Setyaningrum Endah Setyaningrum Endah Setyaningrum Indah Sukma Ningsih Jihan Fikra Angelia Khaleyla, Firas Kusuma Handayani M Kanedi M. Kanedi Meishy Handerlin Putri Meishy Handerlin Putri Mesy Miranda AR Mesy Miranda AR Mutia Dinda Lestari Mutia Dinda Lestari Nismah Nukmal Nismah Nukmal Nismah Nukmal Ratna Claudya Naomi Hutagalung Rizal Koen Asharo Salman Farisi Salman Farisi Siti Inah Sri Wahyuningsih Sumardi Sumardi Sumardi, Sumardi Titik Nur Aeny Titik Nur Aeny Tundjung Tripeni Handayani Ulin Ni'mah Setiawati Ulin Ni'mah Setiawati Vanya Qatrunada Yulianty Yusifa Arsy Variani