Articles
<![CDATA[T cell and B cell reactivities of leprosy patients and their contacts against antigens or epitopes Mycobacterium leprae ]]>
<![CDATA[Hardyanto Soebono, Hardyanto Soebono]]>
<![CDATA[ Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 27, No 03 (1995) ]]>
Publisher : <![CDATA[Journal of the Medical Sciences (Berkala Ilmu Kedokteran) ]]>
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<![CDATA[This study was aimed at evaluating the T cell and B cell reactivity against Mycobacterium leprae antigens or epitopes among leprosy patients and their household contacts in the Indonesian population. Through this study, M. leprae epitopes (either protective or suppresive) will be identified and hopefully proved useful for the development of an effective leprosy vaccine in the future.Fifty-nine leprosy patients consisting of 34 tuberculoid type (TT/BT) and 24 lepromatous type (LUBL) at Dr. Sardjito General Hospital Yogyakarta and 50 household contacts were recruited for this study. After the informed consent was given, 20 ml venous blood was drawn from each subject for assays of the T cell and B cell reactivities. The T cell reactivity was tested by lymphocyte transformation (LTT) and the B cell reactivity was tested serologically by EUSA. M. leprae antigen, PGL-1 and some recombinant proteins (65 kD, 30 kD, 45 kD and 43 kD) were used as antigens in both assays. In addition, Phytohemagglutinin (PHA) and lnterleukin-2 (IL-2) were used as mitogens in the LTT. Statistical analysis was done by using One way ANOVA and Chi-square tests.The results showed that cellular immune deficiency in LUBL patients was found to be specific to the M. leprae antigen, but not to mitogens and other antigens. The T-lymphocyte of the patients (either TT/BT or LUBL) and healthy contacts demonstrated very low reactivities againts all recombinant antigens. On the other hand, the sera of LL/BL leprosy patients reacted significantly against all antigens, most strikingly against PGL-I and 43 recombinant protein of M. leprae. Whereas, the sera of TT/BT patients and healthy contacts showed no or least reactivity against those antigens. These data indicate that although in a small proportion M. leprae recombinant proteins of 65 kD, 30 kD, 45 kD and 43 kD are recognized by T-cell of leprosy patients and healthy contacts. These antigens contain more B-cell epitopes rather than T-cell epitopes. So, these antigens should be eliminated. as soon as a possible candidate in the development of any leprosy vaccine.Key words: leprosy - T-cell and B-cell - Mycobacterium leprae antigen - ELISA - tuberculoid and lepromatouse types]]>
<![CDATA[Association between HLA-DQ alleles and Leprosy in Indonesia Javanese population ]]>
<![CDATA[Hardyanto Soebono, Hardyanto Soebono]]>
<![CDATA[ Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 28, No 01 (1996) ]]>
Publisher : <![CDATA[Universitas Gadjah Mada ]]>
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<![CDATA[Previous studies showed that susceptibility to leprosy and antibody response toward M. leprae in Javanese population was under control of HLA-DR alleles. To investigate whether this susceptibility was also associated with HLA-DQ, the study had been continued with phenotyping of HLA-DQ alleles and antibody assay to the same population which consisted of 79 leprosy patients, 41 tuberculoid (TT/BT) and 38 lepromatous (LL/BL) type, and 50 healthy controls. The HLA-DQ typing had been performed by using a sequence specific oligonucleotyping (SSO). method, while the anti M. leprae antibody had been tested by ELISA and INHIBITION-ELISA.The results show that HLA-DQB501 is associated with leprosy, either tuberculoid or lepromatous type (OR 3.27; 95% Cl 1.42-7.60). When all HLA-DQ1 alleles are analyzed, a significant association is found only with lepromatous leprosy (OR 9.18; 95% Cl 1.89-86.30). IgG antibody anti 36 kD M. leprae is found to be associated with HLA-DQ102. The level of this antibody is higher in HLA-DQ102 positive individuals compared to those negative one (P). No correlation is found between HLA-DQ alleles and the seropositivity of either IgM or IgG.In conclusion, the susceptibility to leprosy in this population is also controlled by genes in HLA-DQ locus. This study also supports the previous findings that HLA-DQ1 is a universal marker for the susceptibility to lepromatous leprosy, while the infection with M. leprae per se is not controlled by HLA genetic factor.Key words : leprosy - M. /eprae - genetic factor - HLA-DQ - HLA-DQ1 - HLA-DQB501]]>
<![CDATA[The role of Various Factors in the Therapeutic Response of Calcipotriol in Mild to Moderate Plaque Type Psoriasis Patients ]]>
<![CDATA[Hardyanto Soebono, Siti Aminah Tri Susila Estri Sunardi Radiono]]>
<![CDATA[ Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 39, No 02 (2007) ]]>
Publisher : <![CDATA[Universitas Gadjah Mada ]]>
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<![CDATA[Background: Psoriasis is a chronic skin inflammation and proliferation disease. Natural history of psoriasis depends on the genetic, age, sex, history of treatment and psychosocial stressor. Calcipotriol is suitable for long-term therapy with good efficacy and safety and clinical response variability but its activity is influenced by ultraviolet.Objective: To know the factors that may influence treatment response of calcipotriol ointment on mild to moderate plaque type psoriasis.Method: Longitudinal study of two times daily calcipotriol ointment 0.005% treatment in the psoriasis patients. Factors that may influence the natural course (age, sex, history of psoriasis in family, history of therapy, MED, working location and psychological stressor) were identified. Evaluation of therapy was performed in the 8th weeks and based on the PASI and PDI. Correlation between treatment responses with various factors were analyzed by using student t-test, Pearson and Spearman correlations and multiple linear regression.Result: At the end of study, PASI was shown to decrease 38.45%, while PDI was 28.70% (p]]>
<![CDATA[Immunopathogenesis of severe acute respiratory syndromecoronavirus 2 (SARS-CoV-2) infection: a concise update ]]>
<![CDATA[Dewi, Shinta Trilaksmi]]>;
<![CDATA[Soebono, Hardyanto]]>
<![CDATA[ Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 52, No 3 (2020) ]]>
Publisher : <![CDATA[Journal of the Medical Sciences (Berkala Ilmu Kedokteran) ]]>
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DOI: 10.19106/JMedSci005203202009
<![CDATA[Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is a novel coronavirus which has been identified as the cause of the recently emerging coronavirus disease 2019 (COVID-19), a respiratory-related infectious disease, in late 2019. As of May 2020, SARS-CoV-2 has infected millions of people with almost 300.000 deaths worldwide only within few months since its first case was reported. While this infection mostly results in mild diseases, the increasing number of severe cases and deaths cannot be overlooked. Due to its novelty, many facets of SARS-CoV-2 pathogenesis are not well understood. This review presents updated knowledge on the key virus characteristics of SARS-CoV-2 and critical notes in the pathogenesis of this viral infection in human that is currently proposed to largely involve various aspects of the host immune responses. While the immediate impact of viral infection in the target cells contributes to the development of the disease, the ability of the virus to modify the host responses may result in the dysregulation of innate and adaptive immune responses, which commonly manifest in the severe spectrum of the disease. Having deep understanding on this complex process is central for tailoring appropriate management for the infected patients as well as for developing effective preventive measures, most importantly vaccine, which is hoped to occur in the near future.]]>
<![CDATA[The role of Various Factors in the Therapeutic Response of Calcipotriol in Mild to Moderate Plaque Type Psoriasis Patients ]]>
<![CDATA[Siti Aminah Tri Susila Estri Sunardi Radiono Hardyanto Soebono]]>
<![CDATA[ Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 39, No 02 (2007) ]]>
Publisher : <![CDATA[Journal of the Medical Sciences (Berkala Ilmu Kedokteran) ]]>
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<![CDATA[Background: Psoriasis is a chronic skin inflammation and proliferation disease. Natural history of psoriasis depends on the genetic, age, sex, history of treatment and psychosocial stressor. Calcipotriol is suitable for long-term therapy with good efficacy and safety and clinical response variability but its activity is influenced by ultraviolet.Objective: To know the factors that may influence treatment response of calcipotriol ointment on mild to moderate plaque type psoriasis.Method: Longitudinal study of two times daily calcipotriol ointment 0.005% treatment in the psoriasis patients. Factors that may influence the natural course (age, sex, history of psoriasis in family, history of therapy, MED, working location and psychological stressor) were identified. Evaluation of therapy was performed in the 8th weeks and based on the PASI and PDI. Correlation between treatment responses with various factors were analyzed by using student t-test, Pearson and Spearman correlations and multiple linear regression.Result: At the end of study, PASI was shown to decrease 38.45%, while PDI was 28.70% (p]]>
<![CDATA[Association between atopy and allergic contact dermatitis in Dr. Sardjito General Hospital Yogyakarta ]]>
<![CDATA[. Fitria]]>;
<![CDATA[Retno Danarti]]>;
<![CDATA[Hardyanto Soebono]]>
<![CDATA[ Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 45, No 02 (2013) ]]>
Publisher : <![CDATA[Journal of the Medical Sciences (Berkala Ilmu Kedokteran) ]]>
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DOI: 10.19106/JMedScie004502201305
<![CDATA[Association between atopy and development of allergic contact dermatitis (ACD) remains controversial. T cell disfunctions in a patient with atopy complicate the process of nickel sensitization. On the other, the decrease of the skin barrier function and overexpression of Langerhans cells in the patient facilitate the sensitization. This study aimed to evaluate the association between atopy and incidence of nickel ACD. A case-control study was carried out in Allergic and Immunology Sub Department of Dermato-Venereology Policlinic, Dr. Sardjito General Hospital, Yogyakarta, involving 54 nickel ACD patients as case group and 74 healthy subjects as control group. All subjects underwent prick test allergens i.e. house dust, dust mite, cockroach, mixed fungi, nuts and egg white. The skin reaction was considered as a positive result if a wheal diameter of at least 3 mm larger than the negative control or a minimum of half of the positive control. The relationship between atopy and the nickel ACD incidence was analyzed using Chi-Square test with confidence interval (CI) of 95%. A significant association between atopy and the nickel ACD incidence was observed in this study. Subjects with atopy to ≥1 allergen had risk of nickel ACD 3.74 higher than subjects without atopy (odds ratio/OR=3.74; 95%CI = 1.64-8.53). Furtheremore, subjects with atopy to ≥2 allergens had risk of nickel ACD 3.74 higher than subjects without atopy (OR=2.08; 95%CI = 1.01-4.29). In conclusion, atopy is a risk factor of nickel ACD.]]>
<![CDATA[Association between environmental allergen sensitization with severity of atopic dermatitis in children and young adult at Dr. Sardjito General Hospital, Yogyakarta ]]>
<![CDATA[Herwinda Brahmanti]]>;
<![CDATA[Niken Trisnowati]]>;
<![CDATA[Retno Danarti]]>;
<![CDATA[Hardyanto Soebono]]>
<![CDATA[ Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 43, No 01 (2011) ]]>
Publisher : <![CDATA[Journal of the Medical Sciences (Berkala Ilmu Kedokteran) ]]>
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<![CDATA[The important role of aeroallergens and food allergens as the most common environmental allergens in exacerbationof atopic dermatitis (AD) iswell known. Sensitization is an essential processwhich correlateswith clinicalmanifestationof AD. The study of AD in Indonesia, especially in Yogyakarta, has not been reported, yet. The aim of study is toevaluate the association between sensitization of environmental allergens with clinical severity of AD in children andyoung adult at Dr. Sardjito General Hospital, Yogyakarta. An analytic observational cross sectional study wasperformed on 33 children and young adult with AD. The severity of disease was determined by SCORing AtopicDermatitis (SCORAD). Assessment of sensitization was performed using specific IgE serum, atopy patch test, andprick test. Data were analyzed using chi-square or Fisher exact test and prevalence ratio (PR) with significancevalue of p<0.05 and 95%confidence interval (CI). The results showed that specific IgE positivity was associatedwith severity of AD. Percentage of specific IgE positivity to house dust mite was significantly higher in subject withmoderate/severe AD compared to those of mild AD (p=0.049; PR 1.13; 95% CI 1.01-1.59). The result was alsosimilar for cat dander (p=0.041; PR 1.1; 95%CI 1.09-4.98), cow’s milk (p=0.038; PR 1.21; 95% CI 1.02-2.2),and egg white (p=0.027; PR 1.23; 95% CI 1.15-2.97). Whereas specific IgE positivity to fish allergen was notstatistically different in subject with moderate/severe AD compared to those with mild AD (p=0.061; PR 0.8; 95%CI 0.76-2.8). According to atopy patch test and prick test result, no association was found between allergensensitization and severity of AD. If allmethodswere combined to increase the sensitivity of sensitizationmeasurement,then the association was found for all allergens. It could be concluded that environmental allergens sensitization isassociated with severity of AD in children and young adult at Dr. Sardjito General Hospital, Yogyakarta.Key words: atopic dermatitis-disease severity-sensitization-aeroallergen-food allergen]]>
<![CDATA[Association between HLA-DQ alleles and Leprosy in Indonesia Javanese population ]]>
<![CDATA[Hardyanto Soebono Hardyanto Soebono]]>
<![CDATA[ Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 28, No 01 (1996) ]]>
Publisher : <![CDATA[Journal of the Medical Sciences (Berkala Ilmu Kedokteran) ]]>
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<![CDATA[Previous studies showed that susceptibility to leprosy and antibody response toward M. leprae in Javanese population was under control of HLA-DR alleles. To investigate whether this susceptibility was also associated with HLA-DQ, the study had been continued with phenotyping of HLA-DQ alleles and antibody assay to the same population which consisted of 79 leprosy patients, 41 tuberculoid (TT/BT) and 38 lepromatous (LL/BL) type, and 50 healthy controls. The HLA-DQ typing had been performed by using a sequence specific oligonucleotyping (SSO). method, while the anti M. leprae antibody had been tested by ELISA and INHIBITION-ELISA.The results show that HLA-DQB501 is associated with leprosy, either tuberculoid or lepromatous type (OR 3.27; 95% Cl 1.42-7.60). When all HLA-DQ1 alleles are analyzed, a significant association is found only with lepromatous leprosy (OR 9.18; 95% Cl 1.89-86.30). IgG antibody anti 36 kD M. leprae is found to be associated with HLA-DQ102. The level of this antibody is higher in HLA-DQ102 positive individuals compared to those negative one (P). No correlation is found between HLA-DQ alleles and the seropositivity of either IgM or IgG.In conclusion, the susceptibility to leprosy in this population is also controlled by genes in HLA-DQ locus. This study also supports the previous findings that HLA-DQ1 is a universal marker for the susceptibility to lepromatous leprosy, while the infection with M. leprae per se is not controlled by HLA genetic factor.Key words : leprosy - M. /eprae - genetic factor - HLA-DQ - HLA-DQ1 - HLA-DQB501]]>
<![CDATA[T cell and B cell reactivities of leprosy patients and their contacts against antigens or epitopes Mycobacterium leprae ]]>
<![CDATA[Hardyanto Soebono Hardyanto Soebono]]>
<![CDATA[ Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 27, No 03 (1995) ]]>
Publisher : <![CDATA[Journal of the Medical Sciences (Berkala Ilmu Kedokteran) ]]>
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<![CDATA[This study was aimed at evaluating the T cell and B cell reactivity against Mycobacterium leprae antigens or epitopes among leprosy patients and their household contacts in the Indonesian population. Through this study, M. leprae epitopes (either protective or suppresive) will be identified and hopefully proved useful for the development of an effective leprosy vaccine in the future.Fifty-nine leprosy patients consisting of 34 tuberculoid type (TT/BT) and 24 lepromatous type (LUBL) at Dr. Sardjito General Hospital Yogyakarta and 50 household contacts were recruited for this study. After the informed consent was given, 20 ml venous blood was drawn from each subject for assays of the T cell and B cell reactivities. The T cell reactivity was tested by lymphocyte transformation (LTT) and the B cell reactivity was tested serologically by EUSA. M. leprae antigen, PGL-1 and some recombinant proteins (65 kD, 30 kD, 45 kD and 43 kD) were used as antigens in both assays. In addition, Phytohemagglutinin (PHA) and lnterleukin-2 (IL-2) were used as mitogens in the LTT. Statistical analysis was done by using One way ANOVA and Chi-square tests.The results showed that cellular immune deficiency in LUBL patients was found to be specific to the M. leprae antigen, but not to mitogens and other antigens. The T-lymphocyte of the patients (either TT/BT or LUBL) and healthy contacts demonstrated very low reactivities againts all recombinant antigens. On the other hand, the sera of LL/BL leprosy patients reacted significantly against all antigens, most strikingly against PGL-I and 43 recombinant protein of M. leprae. Whereas, the sera of TT/BT patients and healthy contacts showed no or least reactivity against those antigens. These data indicate that although in a small proportion M. leprae recombinant proteins of 65 kD, 30 kD, 45 kD and 43 kD are recognized by T-cell of leprosy patients and healthy contacts. These antigens contain more B-cell epitopes rather than T-cell epitopes. So, these antigens should be eliminated. as soon as a possible candidate in the development of any leprosy vaccine.Key words: leprosy - T-cell and B-cell - Mycobacterium leprae antigen - ELISA - tuberculoid and lepromatouse types]]>
<![CDATA[The effect of mitomycin-c in keloid fibroblast cultures ]]>
<![CDATA[Ishandono Dachlan]]>;
<![CDATA[Teguh Aryandono]]>;
<![CDATA[Mae Sri Hartati Wahyuningsih]]>;
<![CDATA[Hardyanto Soebono]]>;
<![CDATA[Yohanes Widodo Wirohadidjojo]]>
<![CDATA[ Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 48, No 3 (2016) ]]>
Publisher : <![CDATA[Journal of the Medical Sciences (Berkala Ilmu Kedokteran) ]]>
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DOI: 10.19106/JMedSci004803201605
<![CDATA[ABSTRACTKeloid occurs due to hyperactivity of keloid fibroblast (KF) in proliferation, migration, collagen deposition, together with low rates of collagen degradation. These are under the responsibility of TGF-b. Mitomycin C (MC) is used for treating keloid by a topical application during surgery at the level of 0.02% to 0.08%. Unfortunately, the lowest effective level of MC for keloid has not been determined yet. We aimed to determine the lowest effective level of MC in the suppression of KF activities. Various levels of MC diluted in growth medium were administered on KF that were isolated from six patients. After 24 hours and 72 hours of incubation, cellular proliferation, collagen deposition, cellular migration and level of TGF-b, were analyzed. Application of 120 uM MC on KF culture for 24 hours could significantly reduce TGF-b production from 1265.74 ± 274.81 pg/mL to 265.17 ± 12.20 pg/mL; proliferation index from 100% to 84.01 ± 12.91%; inhibit cellular migration to 64.38 ± 3.66%; but reduce collagen depositions from 100% to only 91.13 ± 10.19%. The lowest MC level is on 30 uM or equal with 0.001%. In conclusion, the lowest level of MC can suppress the activities of KF is 0.001%. Moreover, due to low activity in inhibiting collagen deposition, MC would be better as an adjuvant drug for keloid surgery.]]>
