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Number of polymorphonuclear leucocytes in the endocervical discharge smear examination as diagnostic test for chlamydial cervicitis Satiti Retno Pudjiati, Satiti Retno Pudjiati
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 29, No 04 (1997)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (155.108 KB)

The chlamydial servicitis is the most prevalent sexually transmitted disease among child-bearing age women and usually asymtomatic in nature, so it is difficult to be diagnosed early and to be eradicated. The laboratory methods for supporting its clinical diagnosis, whether in vitro cultivation or automatic technique, are not only complicated but also expensive. The simplest laboratory testing, especially for primary clinical service with !imitated in laboratory equipment, is achieved by counting the total number of polymorphonuclear leucocytes of endoservical exudate. Unfortunately, the cut off point varied from one country to another. In Indonesia, this cut off point has not determined yet. The purpose of this study was to develop simple and efficient diagnostic test for diagnosing chlamydial cervicitis, by searching the cut off point of a number of polymorphonuclear leucocytes in the endocervical discharge smear examination stained with Gram. The subjects of this study were female sex workers; 44 were from the Clinic of Griya Lentera PKBI Yogyakarta and 36 were from Sanggrahan Resosialisation Yogyakarta. Gynecologic examination, laboratory examination with Gram staining and C. trachomatis antigen detection using Test Pack Chlamydia were done on all subjects. The results indicated that the cut off point of the number of polymorphonuclear leucocyte in diagnosing chlamydial cervicitis is 10 with 60 % sensitivity, 76.67 % specificity, 81 % PPV (positive predictive value) and 53.49 % NPV (negative predictive value).Key words: chlamydial cervicitis - Grams stain - polymorphonuclear leucocyte - test pack chlamydia -female sex workers.

Herpes simplex encephalitis (A review) Satiti Retno Pudjiati, Herning Setijowati
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 40, No 01 (2008)
Publisher : Universitas Gadjah Mada

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Herpes simplex encephalitis (HSEI is an inflammation of the brain caused by herpes simpleks virus (HSVI. HSE includes serious types. HSE can influence all age group, but most common in those under 20 and over 40 years old. Clinical diagnosis based on clinical presentation, laboratory examination of cerebrospinal fluid (CSFI, even by computerized tomography scan (CT-scan)/magnetic resonance imaging (MRII, electro encephalogram (EEGI is difficult that resulted in nonspecific diagnosis. Polymerase chain reaction (PCRI, and brain biopsy are the best diagnostic test. The main treatment of HSE is non spesific i.e. acyclovir. An early management may decrease the risk of mortality from 80% - 25%. While without treatment the mortality.can reach 70-80% and the prognosis might unfavourable.Key words: encephalitis - HSE - CSF - CT-scan - MRI - EEG

Secondary Syphilis, Anal Condylomata Acuminata, and HIV in Bisexual Male Patient: Another Point of View, Time Evaluation Based on CD4 and Management Dewi, Vina Ajeng Puspa; Pudjiati, Satiti Retno
Berkala Ilmu Kesehatan Kulit dan Kelamin Vol 27, No 2 (2015): BIKKK AGUSTUS 2015
Publisher : Faculty Of Medicine Airlangga University

Background: A meta analysis study in China states that male bisexual has higher incidence rate for HIV and syphilis than male-sex-male. Syphilis eases the transmission of HIV, vice versa HIV breaches the integrity of mucosal epithelial barrier, allowing translocation virus and bacterial. Syphilis has higher incidence rates in HIV-infected compare with HIV-uninfected patients. Syphilis influences CD4 of patient. We try discussing a case of secondary syphilis in male bisexual HIV in another point of view, to find which one earlier based on CD4 count. Purpose: to understand another point of view, time evaluation based on CD4 and management in secondary syphilis, anal condyloma accuminata, and HIV in bisexual male patient Case: A 19 years old male bisexual patient complained erythematous rash all over his body, no itch or pain; TPHA 1/2560, VDRL 1/128, positive HIV 3antibody, and CD4 425cell/mm. Case management: HIV onset, considering decrease of CD4 in syphilis or not, are 2-5,8years and 3,08-7,7years, respectively. Therapy was given as single dose benzatin penisilin 2,4billion IU intramuscular. Conclusion:HIV infection occurred before syphilis. No differences in management with HIV-uninfected syphilis patient, more often evaluation interval needed.Key words: syphilis, HIV, bisexual, CD4, onset, management.

Association between sexual orientation and sexual contact with the incidence of human immunodeficiency virus (HIV) infection in Dr. Sardjito General Hospital, Yogyakarta Imtihani, Hajar; Pudjiati, Angela Satiti Retno; Luthfiandi, Mochammad Rifky; Susetiati, Devi Artami
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 51, No 1 (2019)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

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Anal intercourse has been known to have a high risk of human immunodeficiency virus (HIV) transmission. The objective of this study was to evaluate the relationship between sexual orientation and the mode of sexual contact with the incidence of HIV infection. This was observational analytic study with cross sectional design. Subjects were new male patient who visited sexually transmitted infection (IMS) clinics at Dr. Sardjito General Hospital, Yogyakarta during 2016. Data were obtained from medical records. The HIV status was established from HIV rapid test and enzyme-linked fluorescent assay (ELFA). Data were analyzed using descriptive test and Pearson’s chi-squared test with significance level of p< 0.05. Among 167 subjects, 47.91% were in the age group 17 - 25 years old. Forty subjects (24%) had HIV positive status. The majority of subjects were heterosexuals (111 subjects (66.47%)), 43 subjects (25.75%) were homosexuals, and 13 subjects (7.8%) were bisexuals. HIV positive subjects were more common in homosexual groups than were heterosexual and bisexual (p

Erupsi Obat Fikstum Estri, Siti Aminah Tri Susilo; Pudjiati, Satiti Retno
Mutiara Medika: Jurnal Kedokteran dan Kesehatan Vol 3, No 1 (2003)
Publisher : Universitas Muhammadiyah Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18196/mmjkk.v3i1.1550

The fixed drug eruption is one of drug reactions most commonly found.. Clinical pattern of fixed drug eruption (FDE) is characterized by some lessions in the same region (site) for each of the same drug administration. FDE can be diagnosed only based on clinical patterns, from frequent history of drug administration that followed by lessions in the same region. The lessions can vary, including erytema macule or patch followed by an inflammation process and vesicula or bullae in the central lession. To confirm the causa of FDE, it is suggested to conduct a patch test or oral profocation test in several weeks after the lesion resolved. This paper reports the case of the pigmented tipe of FDE with suspected cause of paracetamol or difenhydramin HCl. The lession resolved with metil-prednisolon, mebhidrolin napadisilat and Na Cl compress. The confirmation of the cause of FDE could not be determined because the patch or oral profocation test could not be performed.Erupsi obat fikstum (EOF) merupakan salah satu bentuk reaksi terhadap obat.yang paling sering teijadi. Gambaran klinisnya mempunyai pola khusus, biasanya terjadi pada tempat yang sama setiap kali penderita terpapar obat yang sama dengan bentuk lesi yang sama. Penegakkan diagnosisnya berdasar gambaran klinis, berupa riwayat penggunaan obat yang berulang dan diikuti timbulnya lesi kulit pada daerah yang sama. Lesi kulit dapat bervariasi, mulai makula/patch eritem yang diikuti proses inflamasi sampai terbentuk bula. Untuk memastikan penyebab EOF sebaiknya dilakukan tes tempel atau tes provokasi oral beberapa minggu setelah erupsi obat membaik. Pada makalah ini dilaporkan kasus erupsi obat fikstum (tipe pigmented) dengan kemungkinan penyebab parasetamol atau difenhidramin-HCl. Lesi kulit membaik dengan pemberian metilprednisolon, mebhidrolin napadisilat serta kompres Na Cl. Kepastian penyebab EOF tidak dapat ditemukan karena tidak dapat dikeijakan tes tempel atau profokasi oral.

Erupsi Obat Fikstum Estri, Siti Aminah Tri Susilo; Pudjiati, Satiti Retno
Mutiara Medika: Jurnal Kedokteran dan Kesehatan Vol 3, No 1 (2003)
Publisher : Universitas Muhammadiyah Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18196/mmjkk.v3i1.1550

The fixed drug eruption is one of drug reactions most commonly found.. Clinical pattern of fixed drug eruption (FDE) is characterized by some lessions in the same region (site) for each of the same drug administration. FDE can be diagnosed only based on clinical patterns, from frequent history of drug administration that followed by lessions in the same region. The lessions can vary, including erytema macule or patch followed by an inflammation process and vesicula or bullae in the central lession. To confirm the causa of FDE, it is suggested to conduct a patch test or oral profocation test in several weeks after the lesion resolved. This paper reports the case of the pigmented tipe of FDE with suspected cause of paracetamol or difenhydramin HCl. The lession resolved with metil-prednisolon, mebhidrolin napadisilat and Na Cl compress. The confirmation of the cause of FDE could not be determined because the patch or oral profocation test could not be performed.Erupsi obat fikstum (EOF) merupakan salah satu bentuk reaksi terhadap obat.yang paling sering teijadi. Gambaran klinisnya mempunyai pola khusus, biasanya terjadi pada tempat yang sama setiap kali penderita terpapar obat yang sama dengan bentuk lesi yang sama. Penegakkan diagnosisnya berdasar gambaran klinis, berupa riwayat penggunaan obat yang berulang dan diikuti timbulnya lesi kulit pada daerah yang sama. Lesi kulit dapat bervariasi, mulai makula/patch eritem yang diikuti proses inflamasi sampai terbentuk bula. Untuk memastikan penyebab EOF sebaiknya dilakukan tes tempel atau tes provokasi oral beberapa minggu setelah erupsi obat membaik. Pada makalah ini dilaporkan kasus erupsi obat fikstum (tipe pigmented) dengan kemungkinan penyebab parasetamol atau difenhidramin-HCl. Lesi kulit membaik dengan pemberian metilprednisolon, mebhidrolin napadisilat serta kompres Na Cl. Kepastian penyebab EOF tidak dapat ditemukan karena tidak dapat dikeijakan tes tempel atau profokasi oral.

The combination effect of triamcinolone acetonide and tamoxifen citrate on fibroblast populated collagen lattice contractions Agung Pranoto; Satiti Retno Pudjiati; Yohanes Widodo Wirohadidjojo
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 40, No 02 (2008)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (89.814 KB)

Background: Keloid is caused by fibroblast hyperproliferation stimulated by transforming growth factor-IH ITGF-131 I, and it is usually treated with triamcinolone acetonide (TAl, which has the ability to inhibit TGF131 synthesis. However, the clinical results is still unsatisfied. Another drug that may inhibit keloid fibroblast TGF-131 synthesis is tamoxifen citrate (TCI, but the effect of the combination on keloid fibroblast activities has never been published.Objective: To find out the effect of combined triamcinolone acetonide and tamoxifen citrate on fibroblast keloid activities in vitro.Methods: It was a parallel post-test only study. The third passage keloid fibroblasts were isolated from a patient with keloid, cultivated in collagen lattice, and treated with several combinations of 5, 10, and 20 pM TA and 10, and 20 pM TC. Lattice contractions were measured based on digital image using scion image.Results: Among TA groups, the best inhibition of lattice contraction was found among 20 pM treated group and among TC groups. The best inhibition of lattice contraction was found among 20 pM TC. The best combination was found in the combination of 20 pM TA plus 20 pM TC.Conclusion: The result indicated that a combination of triamcinolone acetonide and tamoxifen citrate had a significant role in suppressing fibroblast activity, better than triamcinolone acetonid or tamoxifen citrate alone.Key words: tamoxifen - triamcinolone - collagen lattice - keloid fibroblast.

Herpes simplex encephalitis (A review) Herning Setijowati Satiti Retno Pudjiati
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 40, No 01 (2008)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

Show Abstract | Download Original | Original Source | Check in Google Scholar

Herpes simplex encephalitis (HSEI is an inflammation of the brain caused by herpes simpleks virus (HSVI. HSE includes serious types. HSE can influence all age group, but most common in those under 20 and over 40 years old. Clinical diagnosis based on clinical presentation, laboratory examination of cerebrospinal fluid (CSFI, even by computerized tomography scan (CT-scan)/magnetic resonance imaging (MRII, electro encephalogram (EEGI is difficult that resulted in nonspecific diagnosis. Polymerase chain reaction (PCRI, and brain biopsy are the best diagnostic test. The main treatment of HSE is non spesific i.e. acyclovir. An early management may decrease the risk of mortality from 80% - 25%. While without treatment the mortality.can reach 70-80% and the prognosis might unfavourable.Key words: encephalitis - HSE - CSF - CT-scan - MRI - EEG

Number of polymorphonuclear leucocytes in the endocervical discharge smear examination as diagnostic test for chlamydial cervicitis Satiti Retno Pudjiati Satiti Retno Pudjiati
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 29, No 04 (1997)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (155.108 KB)

The chlamydial servicitis is the most prevalent sexually transmitted disease among child-bearing age women and usually asymtomatic in nature, so it is difficult to be diagnosed early and to be eradicated. The laboratory methods for supporting its clinical diagnosis, whether in vitro cultivation or automatic technique, are not only complicated but also expensive. The simplest laboratory testing, especially for primary clinical service with !imitated in laboratory equipment, is achieved by counting the total number of polymorphonuclear leucocytes of endoservical exudate. Unfortunately, the cut off point varied from one country to another. In Indonesia, this cut off point has not determined yet. The purpose of this study was to develop simple and efficient diagnostic test for diagnosing chlamydial cervicitis, by searching the cut off point of a number of polymorphonuclear leucocytes in the endocervical discharge smear examination stained with Gram. The subjects of this study were female sex workers; 44 were from the Clinic of Griya Lentera PKBI Yogyakarta and 36 were from Sanggrahan Resosialisation Yogyakarta. Gynecologic examination, laboratory examination with Gram staining and C. trachomatis antigen detection using Test Pack Chlamydia were done on all subjects. The results indicated that the cut off point of the number of polymorphonuclear leucocyte in diagnosing chlamydial cervicitis is 10 with 60 % sensitivity, 76.67 % specificity, 81 % PPV (positive predictive value) and 53.49 % NPV (negative predictive value).Key words: chlamydial cervicitis - Gram's stain - polymorphonuclear leucocyte - test pack chlamydia -female sex workers.

Low CD4+ T cell counts are not risk factor for Malassezia species infection in HIV/AIDS patients Epi Panjaitan; Satiti Retno Pudjiati; Agnes Sri Siswati
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 46, No 04 (2014)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

Human immunodefiiency virus (HIV) infection and aquired immunodeficiency syndrome (AIDS)cause a progressive depletion of CD4+ T cell populations accompanied by progressive impairmentof cellular immunity and increasing susceptibility to opportunistic infections. Seborrheic dermatitisis one of the most common skin opportunistic infections on HIV/AIDS patients. Malasseziaspecies is bilieved as the causative of seborrheic dermatitis. The aim of the study was to evaluatelow CD4+ T cell counts as risk factor for Malassezia sp. infection in HIV/AIDS patients. This wasan observational study with cross-sectional design conducted on HIV/AIDS patients who attendedin Department of Dermatology and Venereology, Faculty of Medicine Universitas Gadjah Mada/Dr Sardjito General Hospital, Yogyakarta and met the inclusion and exclusion criteria. Culture ofMalassezia sp. was conducted in Department of Microbiology and classified as high (>100 CFU/tape) and low (<100 CFU/tape) density colonies. CD4+ T cell counts were measured in Departmentof Clinical Pathology and classified as high (>200 cells/mm3) and low (<200 cells/mm3) CD4+ Tcell counts. A total of 83 subjects with HIV/AIDS comprising 54 (65.1%) males and 29 (34.9%)females aged 20 - >60 years were involved in the study. The number of Malassezia sp. colonyon subjects with high and low CD4+ T cell counts were 31.55 ± 26.21 and 25.2 ± 33.89 CFU/tape, respectively. No significantly relationship between between CD4+ T cell count and Malasseziasp. colony number was observed in the study (p=0.607; 95%CI=0.04-5.19; RP=0.452). Inconclusion, low CD4+ T cell counts is not risk factor for Malassezia sp. infection in HIV/AIDSpatients.

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