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All Journal Folia Medica Indonesiana Jurnal Biometrika dan Kependudukan (Journal of Biometrics and Population) Biomolecular and Health Science Journal JUXTA: Jurnal Ilmiah Mahasiswa Kedokteran Universitas Airlangga
Faisal Yusuf Ashari
Department Of Medical Biology, Faculty Of Medicine, Universitas Airlangga, Surabaya
Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology Neuroscience Public Health Other

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Human Sperm Motility, Viability, and Morphology Decreased after Cryopreservation Ninik Darsini; Berliana Hamidah; Seso Sulijaya Suyono; Faisal Yusuf Ashari; R Haryanto Aswin; Rina Yudiwati
Folia Medica Indonesiana Vol. 55 No. 3 (2019): September
Publisher : Faculty of Medicine, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (151.624 KB) | DOI: 10.20473/fmi.v55i3.15501

Abstract

The aim of this study was to analyze human sperm motility, viability, and morphology before and after cryopreservation. This true laboratory experimental study had pre and post randomized one group design. The study was conducted at the Embryology, Andrology, and Genetics Laboratory, Department of Medical Biology, Faculty of Medicine, Universitas Airlangga from August to November 2017. The eighteen samples of fresh semen were collected from male volunteers who agreed and signed the informed consent of the study. Samples were analyzed their motility, viability, and morphology before and after cryopreservation. Results of this study indicated differentiation between motility before and after cryopreservation. Cryopreservation process decreased progressive motility (42.22 + 9.46%; 17.83 + 6.24%; p< 0.0001) and increased the number of immotile spermatozoa (35.44 + 10.15%; 60.11 + 12.53%; p< 0.0001). Cryopreservation also decreased human sperm viability (73.78 + 8.91%; 40.83 + 12.89%; p< 0.0001) and morphology (10.94 + 4.96%; 7.39 + 3.90%; p< 0.0001). Cryopreservation of human spermatozoa caused the decreased of motility, viability, and morphology.
The Effect of Antioxidant Vitamin C and E Administration on Spermatozoa Quality of White Rat (Rattus Norvegicus) Strain Wistar Male Against Allethrin Exposure Faisal Yusuf Ashari; Rina Yudiwati; R.Haryanto Aswin
Biomolecular and Health Science Journal Vol. 1 No. 2 (2018): Biomolecular and Health Science Journal
Publisher : Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (379.846 KB) | DOI: 10.20473/bhsj.v1i2.9282

Abstract

Introduction: Allethrin is an active ingredient used in anti-mosquito drugs. Allethrin enters the body through inhalation, allegedly can disrupt the quality of spermatozoa by decreasing its amount, motility, viability and morphology. Many studies suggested that with sufficient intake of vitamin C and E would be able to minimize free radicals caused by allethrin. This study aims to investigate the effect of giving antioxidant vitamin C and E on spermatozoa quality of white rat (Rattus norvegicus) strain Wistar male with allethrin exposure.Methods: This research was a post-test design randomized control group design using Rattus novergicus male strains Wistar exposed to allethrin (LPB IPB veterinary stem cell). A total of 16 white rat strains Wistar male divided into 4 groups, one control group (without any treatment) and 3 treatment groups (given antioxidant vitamin C, vitamin E and both). The exposure of mosquito coils was performed 8 hours per day for 45 days and on the 46th day, spermatozoa was observed. The data were analyzed using ANOVA one way and BNT.Results: The results showed that treatment with vitamins affected the amount, motility and viability of rat spermatozoa exposed to allethrin. The mean amount of control group spermatozoa and treatment was 14,93, the mean spermatozoa motility control and treatment groups were 45,13 and the mean spermatozoa viability of control and treatment groups was 58,88. This is shown by the real difference between the control group and the treatment (p <0,05). Similarly, in the control group found morphological abnormalities.Conclusion: Exposure of mosquito coils 8 hours/day for 45 days decreased the spermatozoa quality. Overall, the admission of antioxidant vitamin C and E are able to maintain the quality of spermatozoa instead of one type of vitamin.
The Antibacterial Activity of Amber Honey and White Honey on Pseudomonas aeruginosa Multi Resistant (PaMR) and Methicillin Resistant Staphylococcus aureus (MRSA) Faisal Yusuf Ashari; Setio Harsono; Manik Retno Wahyunitisari
JUXTA: Jurnal Ilmiah Mahasiswa Kedokteran Universitas Airlangga Vol. 11 No. 2 (2020): Jurnal Ilmiah Mahasiswa Kedokteran Universitas Airlangga
Publisher : Faculty of Medicine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/juxta.V11I22020.74-78

Abstract

Introduction: Infection of burn wound is a chronical disturbance to the skin, which is caused by Staphylococcus aureus and Pseudomonas aeruginosa, but lately many cases found that both bacteria have been resistant to the antibiotic. To overcome the infection caused by those bacteria, it is important to search for natural substance that has antibacterial activity to both bacteria. The power of natural substance is underestimated. Studies reveal that honey can be more effective for healing burns than modern medicine. The previous research revealed that amber honey and white honey were used as an alternative treatment against Pseudomonas aeruginosa Multi Resistant (PaMR) and Methicillin Resistant Staphylococcus aureus (MRSA). The aim of this study was to evaluate the antibacterial activity of amber honey and white honey on PaMR and MRSA.Methods: This research was conducted by performing antibacterial test phase, determining Minimum Inhibitory Concentration (MIC), and determining comparison value of antibacterial activity from amber honey and white honey to tetracycline.Results: The result of this research showed that both amber honey and white honey have antibacterial activity to both bacteria, by way of stronger antibacterial activity from white honey than amber honey. Amber honey and white honey have the same PaMR activity. MIC of white honey was 7.1% (to MRSA) and 12.3% (to PaMR), while MIC of amber honey was 10% (to MRSA) and 12.5% (to PaMR). The comparison value of antibacterial activity between amber honey and white honey to tetracycline for MRSA was equal to 1:1.08 x 10-4 and 1:1.62 x 10-4. The comparison value of antibacterial activity between amber honey and white honey to tetracycline for PaMR was equal to 1:5.62 x 10-6 and 1:1.03 x 10-5 .Conclusion: Amber honey and white honey have antibacterial activity against MRSA and PaMR. 
MODELING OF MATERNAL MORTALITY (MMR) IN EAST JAVA 2017-2019 USING PANEL REGRESSION APPROACH Febriyanto, Eka Cahya; Indawati, Rachmah; Mahmudah, Mahmudah; Ama, Fuad; Ashari, Faisal Yusuf
Jurnal Biometrika dan Kependudukan (Journal of Biometrics and Population) Vol. 12 No. 2 (2023): JURNAL BIOMETRIKA DAN KEPENDUDUKAN
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbk.v12i2.2023.177-185

Abstract

Maternal Mortality Rate (MMR), is a country's welfare indicator especially about the women's health. It is included in the development index and the quality of life index. The determinants of maternal mortality are elements that collectively cause maternal mortality. This quantitative research used secondary data on MMR and its influencing factors in East Java Province during the 2017-2019 period and the analysis of the panel data used regression. The trend of Maternal mortality in East Java Province has decreased from year to year; in 2019, it was 89.81 per 100,000 live births. The variable pregnant women using blood-added pills (X1) with a probability value of 0.020 (p<0.05) is found to significantly affected the maternal mortality in the panel regression analysis utilizing the Random Effect Model. The Random Effect Model, which this study found to be the best, contains the model equation Yit = 225.7721 - 1.919457 X1it and the administration of blood-added pills is the variable that significantly affects maternal mortality. The results of this study indicate the need for educational efforts, especially regarding to the benefits of blood-added pills for pregnant women and to correct the misunderstanding about the benefits of blood-added pills in the community, so that the coverage of blood supplement consumption in pregnant women can be increased. Together with the primary health care system and obstetricians, an integrative approach and counseling among pregnant women and their partners will increase awareness of maternity health and birth management.
Co-Authors Ama, Fuad Aswin, R. Haryanto Berliana Hamidah Eka Cahya Febriyanto Mahmudah Mahmudah ManikRetno Wahyunitisari Ninik Darsini Rachmah Indawati RINA YUDIWATI BM, RINA Seso Sulijaya Suyono Setio Harsono