Article Per Year (5 Year)
p-Index From 2021 - 2026
0.23
P-Index
This Author published in this journals
All Journal Sewagati Folia Medica Indonesiana
Darsini, Ninik
Unknown Affiliation
Arts Humanities Biochemistry, Genetics & Molecular Biology Education Engineering Health Professions Languange, Linguistic, Communication & Media Medicine & Pharmacology Neuroscience Public Health Social Sciences Other

Published : 3 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 3 Documents
Search

 1 
Human Sperm Motility, Viability, and Morphology Decreased after Cryopreservation Darsini, Ninik; Hamidah, Berliana; Suyono, Seso Sulijaya; Ashari, Faisal Yusuf; Aswin, R Haryanto; Yudiwati, Rina
Folia Medica Indonesiana Vol. 55, No. 3
Publisher : Folia Medica Indonesiana

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

The aim of this study was to analyze human sperm motility, viability, and morphology before and after cryopreservation. This true laboratory experimental study had pre and post randomized one group design. The study was conducted at the Embryology, Andrology, and Genetics Laboratory, Department of Medical Biology, Faculty of Medicine, Universitas Airlangga from August to November 2017. The eighteen samples of fresh semen were collected from male volunteers who agreed and signed the informed consent of the study. Samples were analyzed their motility, viability, and morphology before and after cryopreservation. Results of this study indicated differentiation between motility before and after cryopreservation. Cryopreservation process decreased progressive motility (42.22 + 9.46%; 17.83 + 6.24%; p< 0.0001) and increased the number of immotile spermatozoa (35.44 + 10.15%; 60.11 + 12.53%; p< 0.0001). Cryopreservation also decreased human sperm viability (73.78 + 8.91%; 40.83 + 12.89%; p< 0.0001) and morphology (10.94 + 4.96%; 7.39 + 3.90%; p< 0.0001). Cryopreservation of human spermatozoa caused the decreased of motility, viability, and morphology.
Fertilization of bovine oocytes vitrified pre- and post in vitro maturation Faizah, Zakiyatul; Darsini, Ninik; Hinting, Aucky
Folia Medica Indonesiana Vol. 52, No. 2
Publisher : Folia Medica Indonesiana

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

The success rate of fertilization post save frozen oocytes is still very low, because the oocyte has distinctive features, namely the volume ratio and a lower surface to the limited penetration of water and cryoprotectants penetrate cells. Beside mature oocytes have a thread spindles are particularly vulnerable to the drop in temperature. Keep frozen oocytes is needed, especially in women who needed rescue fertility so their oosit can be fertilized. Maturation is done in TC 100 mL medium covered with mineral oil in a petri dish with a diameter of 36 mm. Oocyte vitrification begins with washing in PBS supplemented medium serum 20% for 1-2 minutes, followed by serum in the medium PBS + 20% + 10% ethylene glycol for 10-14 minutes. Then oocyte vitrification medium is transported in PBS + serum 20% + sucrose 0.5M ethylene glycol + 15% + 15% PROH for 25-30 seconds. Thawing oocytes is done by successive immersed in the media: 1). PBS + 20% serum + 0.5M sucrose, 2). PBS + 20% serum + 0.25M sucrose, and 3). PBS + 20% serum + 0.1 M sucrose. Insemination is done in rosset, and the number of fertilization was observed after 48 hours. Fertilization in the control group amounted to 42.97%, while the K1 and K2 there are no fertilization at all. The analysis showed that fertilization in the control and treatment groups significantly different at p <0.05 in both treatment groups K1 or K2 there are no fertilization at all. The conclusions of this study is there is no difference between the amount of fertilization of bovine oocytes were vitrified pre and post-maturation in vitro.
Upaya peningkatan pola pengasuhan guru dan orang tua penyandang disabilitas di Pendidikan Khusus dan Layanan Khusus (PK-LK) Shafa Mojokerto Rimbun, Rimbun; Yuliawati, Tri Hartini; Faizah, Zakiyatul; Hamidah, Berliana; Amanda, Bella; Prasetiowati, Lucky; Sari, Dewi Ratna; Purwantari, Kusuma Eko; Wungu, Citrawati Dyah Kencono; Darsini, Ninik; Fepiosardi, Raihenette Amirasanti; Fathimatuzzahroh; Parmanto, Christian Melka
Sewagati Vol 9 No 5 (2025)
Publisher : Pusat Publikasi ITS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.12962/j26139960.v9i5.8095

Abstract

Anak Berkebutuhan Khusus (ABK) adalah anak yang mengalami keterbatasan ataukeluarbiasaan, baik fisik, mental-intelektual, sosial, maupun emosional. Keberadaanpendamping bagi siswa ABK memiliki makna yang berarti bagi proses perlindungandan tumbuh kembangnya. Kegiatan pengabdian kepada masyarakat ini bertujuanuntuk meningkatkan pengetahuan guru dan orang tua mengenai pola asuh bagi siswapenyandang disabilitas di Sekolah Shafa Mojokerto. Sebanyak 23 siswa berkebutuhankhusus diberikan asesmen kognitif, sosioemosional dan psikomotor lengkapdengan saran bagi stimulasi tumbuh kembangnya, yang dilakukan oleh psikologklinis dari Layanan Psikologi Rumah Kumbang. Diantaranya, sebanyak 4 siswapenyandang tuna daksa (cerebral palsy) diberikan asesmen fisik yang dilakukan olehDokter Spesialis Kedokteran Fisik dan Rehabilitasi dari RSUD Dr. Soetomo. Selainitu juga diberikan penyuluhan mengenai cerebral palsy, pemeriksaan genetik danpola pengasuhan yang tepat bagi guru dan orang tua siswa. Luaran dari kegiatanPkM yang telah tercapai antara lain tersedianya laporan asesmen psikologis siswadan peningkatan pengetahuan guru dan orang tua yang dibuktikan dengan peningkatanrerata nilai posttest test (80, 63 ± 14, 36) dibanding pretest (61, 88 ± 20, 07)secara signifikan ( ? = 0, 010). Kesiapan dan kesiagaan guru dan orang tua siswaABK merupakan kunci sukses penanganan, ditambah dukungan dari masyarakat danpemerintah dalam menyediakan lingkungan dan fasilitas yang ramah terhadap ABK.
Co-Authors Amanda, Bella Aswin, R Haryanto Aucky Hinting Dewi Ratna Sari Faisal Yusuf Ashari Faizah, Zakiyatul Fathimatuzzahroh Fepiosardi, Raihenette Amirasanti Hamidah, Berliana Kusuma Eko Purwantari Lucky Prasetiowati Parmanto, Christian Melka Rimbun, Rimbun RINA YUDIWATI BM, RINA Suyono, Seso Sulijaya Wungu, Citrawati Dyah Kencono Yuliawati, Tri Hartini