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All Journal Prosiding Seminar Nasional Multidisiplin Cendekia : Jurnal Pengabdian Masyarakat Jurnal Agriovet Prosiding Conference on Research and Community Services Jumat Pertanian: Jurnal Pengabdian Masyarakat AGARICUS: Advances Agriculture Science & Farming Agrosaintifika: Jurnal Ilmu-Ilmu Pertanian
Umi Kulsum Nur Qomariah
Universitas KH. A. Wahab Hasbullah
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Electrical & Electronics Engineering Environmental Science Languange, Linguistic, Communication & Media Mathematics Social Sciences Other

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Optimization of Fruit DNA Extraction by Kitchen Kit Method with Isopropanol and Absolute Ethanol Umi Kulsum Nur Qomariah
AGARICUS: Advances Agriculture Science & Farming Vol. 3 No. 1 (2023): June
Publisher : LPPM Universitas KH. A. Wahab Hasbullah

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32764/agaricus.v3i1.3879

Abstract

soft fruits are high-value food commodities that are in great demand by the wider community, so they have the potential to be developed. It is necessary to study various scientific disciplines, one of which is the molecular biology approach. DNA is a basic element in molecular biology research. The DNA extraction technique greatly determines the quality and quantity of the DNA produced. The chemical solvent used in the precipitation is an important factor in producing the quality and quantity of DNA, so optimization is required. The purpose of the research to investigate the effect of isopropanol and ethanol on fruit DNA extraction. The DNA extraction method used is the kitchen kit method which is used on 4 types of soft fruit: papaya, guava, banana and strawberry. The principles of DNA extraction are lysis, precipitation and purification. The lysis process was carried out chemically with a solution of detergent and NaCl, and physically by means of a blender, then separation was carried out using filter paper. The aquos phase is collected to be chemically precipitated. Precipitation was carried out with cold absolute ethanol and cold isopropanol at -40C. The results of extraction with isopropanol showed the consistency and quantity of fruit DNA: papaya had rather dense fiber, slightly soft guava, slightly faded thin strawberry and rather dense fibrous banana. The results of absolute ethanol extraction showed the consistency of fruit DNA: papaya fiber rather dense, guava fiber medium, strawberry rather dense and banana fiber moderate. DNA extraction by ethanol precipitation produces more optimal DNA compared to isopropanol precipitation.
EFEKTIVITAS AIR KELAPA MUDA DAN TUA SEBAGAI ZPT ALAMI PADA PERTUMBUHAN JAMUR TIRAM PUTIH (Pleurotus ostreatus) Maria ulfa; Umi Kulsum Nur Qomariah; Anggi Indah Yuliana
Jurnal Agriovet Vol. 6 No. 2 (2024): JURNAL AGRIOVET
Publisher : LPPM UNIVERSITAS KAHURIPAN KEDIRI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51158/agriovet.v6i2.1164

Abstract

Saat ini jamur tiram putih (Pleurotus ostreatus) termasuk salah satu jenis jamur yang sangat terkenal di kalangan khalayak umum. Jamur ini selain mudah dibudidayakan, juga memiliki daya tarik ekonomi yang tinggi sehingga menjadikan keuntungan yang menjanjikan bagi para penggarap. Salah satu cara dari banyaknya cara untuk memajukan penghasilan jamur tiram putih adalah menggunakan zat pengatur tumbuh alami (ZPT) seperti air kelapa muda atau tua. Tujuan penelitian ini untuk mengetahui pengaruh pemberian kedua ZPT alami tersebut terhadap pertambahan bobot basah, diameter tutup jamur, dan jumlah tutup jamur. Penelitian ini dilakukan dengan menggunakan rancangan acak lengkap (RAL) sederhana dimana tindakan yang dilakukan diberikan air kelapa muda sebanyak 3ml dan 6ml serta air kelapa tua sebanyak 3ml dan 6ml setiap 2 hari sekali, serta diberikan air suling sebagai kontrol. Suhu di Kumbung dipertahankan antara 26 dan 30°C dan kelembaban relatif antara 80 dan 90%. Parameter yang diamati meliputi berat basah, diameter tutup jamur, dan jumlah tutup jamur. Hasil penelitian menunjukkan bahwa perlakuan T6 menghasilkan rata-rata jumlah kapsul 13,2, diameter tutup 10,7 cm, dan berat basah jamur 73,4 g, namun perbedaannya tidak nyata dibandingkan perlakuan Tugas akhir lainnya.
Co-Authors . Winarko Anggi Indah Yuliana Anggun Wulandari Darmawan, Moh Faridl Dian Kusuma Wardani Endang Semiarti ima alimah alimah Latifatul Ulla M Arsyad Amrullah Maria ulfa Mar’atul Fahimah Mazidatul Faizah Miftachul Chusna Mis Suhartini Moh Andrik Robil Alamsyah Muhammad Ega Saputro Nanda Risky Ardiana Sindi Yulia Anggraini Siti Aminatuz Zuhria Siti Nur Qomariyah Sri Bayuputra Iwana Iksan Suci Prihatiningtyas Surya Andre Ferdian Causa Vina Ainul Bashiroh