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All Journal HAYATI Journal of Biosciences Jurnal Kedokteran Brawijaya Research Journal of Life Science Jurnal Respirologi Indonesia Narra J
Dian Nugrahenny
Department Of Pharmacology, Faculty Of Medicine, Universitas Brawijaya, Malang, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Health Professions Immunology & microbiology Medicine & Pharmacology Public Health

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Vitamin E Mempertahankan Kemampuan EPC yang Dipapar Glukosa Tinggi dalam Pelepasan NO dan Induksi Migrasi Sel Endotel Nugrahenny, Dian; Widodo, M Aris; Permatasari, Nur
Jurnal Kedokteran Brawijaya Vol 27, No 1 (2012)
Publisher : Fakultas Kedokteran Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1004.234 KB) | DOI: 10.21776/ub.jkb.2012.027.01.2

Abstract

Peran sel progenitor endotel (EPC) dalam angiogenesis terganggu pada diabetes. Penelitian dilakukan untuk mengamati efek  vitamin  E  pada  kemampuan  EPC  yang  dipapar glukosa  tinggi  dalam melepaskan  NO dan  menginduksi  migrasi  sel endotel.  Sel mononuklear diisolasi dari darah perifer subjek sehat.  Pada hari ke-7, kultur EPC diberikan glukosa normal (5 mM) dengan atau tanpa pemberian vitamin E 22 µM atau 50 µM sebagai kontrol, atau diberikan glukosa tinggi (22 mM) dengan  atau  tanpa  pemberian  vitamin  E  22  µM  atau  50  µM  selama  24  jam.  Fungsi  EPC  dinilai  dengan  mengevaluasi  migrasi HUVEC setelah pemberian supernatan EPC. Migrasi HUVEC dinilai dengan uji migrasi wound-healing. Konsentrasi NO dan H O   EPC diukur dengan uji kolorimetrik.  Superoksid EPC dinilai dengan uji NBT .  Pemberian glukosa tinggi mengakibatkan 2 2penurunan kemampuan EPC dalam menginduksi migrasi HUVEC, penurunan NO EPC, serta peningkatan superoksid dan H O   EPC.  Pemberian  vitamin  E  50  µM  dapat  menghambat  penurunan  kemampuan  EPC  dalam  menginduksi  migrasi 2 2HUVEC,  dan  efek  ini  terkait  dengan  konsentrasi  NO,  superoksid  dan  H O   EPC.  Pemberian  vitamin  E  dapat 2 2mempertahankan kemampuan EPC yang dipapar glukosa tinggi dalam melepaskan NO dan menginduksi migrasi HUVECs melalui  hambatan  peningkatan  superoksid  dan  H O   EPC.2 2Kata  Kunci:  EPC,  glukosa  tinggi,  NO,  ROS,  vitamin  E
Subchronic Toxicity of the Physalis minima Leaves Setyawati Soeharto; Dian Nugrahenny; Nur Permatasari; Elly Mayangsari
Research Journal of Life Science Vol 5, No 1 (2018)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (315.343 KB) | DOI: 10.21776/ub.rjls.2018.005.01.8

Abstract

The study was to evaluate the subchronic toxicity of aqueous extract of Physalis minima leaves in female rats. Eighteen female Wistar rats were orally administered with aqueous extract of P. minima leaves at doses of 90, 270 and 450 mg/kg BW, respectively for 90 days. Six female rats without any treatment were provided as a control group. At the end of the experiment, blood samples were collected for hematological and biochemical (aspartate transaminase/AST, alanine transaminase/ALT, creatinine, urea, glucose, cholesterol, and triglyceride) analyses. The results showed that hematological and biochemical biomarkers were not significantly different in treated groups compared to the control group. It is concluded that the aqueous extract of P. minima leaves do not induce hematologic toxicity, hepatotoxicity and renal toxicity in the subchronic toxicity study.
The Differences in Urokinase Plasminogen Activator System in Lung Cancer Patients Before and After Chemotherapy Tri Wahju Astuti; Agustin Iskandar; Mufidatun Hasanah; Lindayanti Sumali; Dian Nugrahenny
Jurnal Respirologi Indonesia Vol 41, No 4 (2021)
Publisher : Perhimpunan Dokter Paru Indonesia (PDPI)/The Indonesian Society of Respirology (ISR)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36497/jri.v41i4.214

Abstract

Background: Lung cancer is still the leading cause of death for malignancies worldwide. Urokinase plasminogen activator (uPA), its soluble receptor (suPAR), and its inhibitor (PAI-1) play an important role in tumor invasion and metastasis. This study aimed to evaluate the differences in the urokinase plasminogen activator system (uPA, suPAR, and PAI-1) in lung cancer patients before and after chemotherapy.Methods: This research was an observational analytical study with a cross-sectional design. The subjects were 30, consisting of 17 lung cancer patients before chemotherapy and 13 lung cancer patients after chemotherapy for 4 or 6 cycles. The levels of serum uPA, suPAR, and PAI-1 were measured by enzyme-linked immunosorbent assay (ELISA). Results: In lung cancer patients before chemotherapy, there were no significant (p>0.05) differences in levels of serum uPA, suPAR, and PAI-1 between patients with stage III and IV. The highest serum uPA and suPAR levels were found in adenocarcinoma cell types and the highest serum PAI-1 level in adenoepidermoid cell types. After chemotherapy, serum suPAR and PAI-1 were significantly (p < 0.05) decreased in lung cancer patients. However, there were no significant (p>0.05) differences in the levels of serum uPA, suPAR, and PAI-1 between patients with chemotherapy responses for stable and progressive diseases. Conclusion: This study revealed that suPAR and PAI-1 levels were decreased in lung cancer patients who had received chemotherapy. This can occur due to decreased tumor cells activity. 
Physalis angulata Leaf Ethanol Extract Reduces Oxidative Stress and Improves Endothelial Progenitor Cells in L-NAME-Induced Hypertensive Rats Dian Nugrahenny; Nur Permatasari; Setyawati Soeharto; Indriati Dwi Rahayu; Edwin Widodo; Karyono Mintaroem; Hidayat Sujuti; Retty Ratnawati; Elly Mayangsari; Dicky Faizal Irnandi; Lavina Sofia Ardani; Tika Ardhini Wardoyo; Nathania Bella Claresta; Nura Fattah Cantika Yoga; Adinda Chika Anindita; Niarti Ulan Sari Siarnu; Fardizia Putri Alia
HAYATI Journal of Biosciences Vol. 30 No. 1 (2023): January 2023
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.30.1.81-87

Abstract

This study aimed to evaluate the effects of ciplukan (Physalis angulata L.) leaf ethanol extract on L-NG-nitroarginine methyl ester (L-NAME)-induced hypertensive rats. We randomly divided twenty-five Wistar rats into five groups. The sham group was given a PBS injection. The hypertensive group was injected with L-NAME on days 1 to 28. Three groups of hypertensive rats were given the extract on days 4 to 28. Blood pressure was measured using the tail-cuff method on days 0, 4, 10, and 27. The endothelial progenitor cells (EPCs) in the blood were measured by flow cytometry as a percentage of circulating angiogenic cells (CACs, CD34+/CD309+/CD45+) and endothelial colony-forming cells (ECFCs, CD34+/CD309+/CD45-). Serum NO and MDA levels, as well as serum SOD activity, were measured colorimetrically. Serum TNF-α levels were measured by the ELISA method. The ciplukan leaf extract reduced systolic and diastolic blood pressure, reduced the percentage of EPCs in the blood, increased serum NO levels, reduced MDA levels, increased serum SOD activity, and reduced serum TNF-α levels in L-NAME-induced hypertensive rats. It is concluded that ciplukan ethanol leaf extract exerts protective effects on L-NAME-induced hypertensive rats. These study results can strengthen the scientific basis of using ciplukan leaf ethanol extract to treat hypertension.
Inhibition of B-cell activating factor activity using active compounds from Physalis angulata in the mechanism of nephrotic syndrome improvement: A computational approach Kardani, Astrid K.; Fitri, Loeki E.; Samsu , Nur; Subandiyah , Krisni; Endharti, Agustina T.; Nugrahenny, Dian; Wibowo, Syahputra
Narra J Vol. 4 No. 3 (2024): December 2024
Publisher : Narra Sains Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52225/narra.v4i3.859

Abstract

Nephrotic syndrome, a multifaceted medical condition characterized by significant proteinuria, has recently prompted a reorientation of research efforts toward B-cell-mediated mechanisms. This shift underscores the pivotal role played by B-cells in its pathogenesis. The aim of this study was to explore potential therapeutic pathways, with specific attention given to compounds found in Physalis angulata, including withanolides, such as physalins, which constitute one of the five distinct withanolide subgroups identified in Physalis angulata. Furthermore, the study assessed the monoclonal antibody belimumab, designed to target B-cell activating factor (BAFF) and its associated receptors (TACI, BCMA, and BAFF-R). Various research techniques were employed, encompassing data mining, bioactivity analysis, Absorption, Distribution, Metabolism, Excretion, and Toxicity (ADMET) profiling, molecular modeling, and docking studies. Withanolide was demonstrated as a potential inhibitor for the protein BAFF, showing a binding energy of -7.1 kcal/mol. Physalin F emerged as the leading candidate inhibitor for the protein TACI, with a binding energy of -8.3 kcal/mol. Similarly, withanolide was identified as the top inhibitor candidate for the protein BCMA, exhibiting a binding energy of -7.0 kcal/mol. The most favorable interaction with BAFF-R was physalin F, which displayed a binding energy of -8.0 kcal/mol. Moreover, molecular dynamic simulation suggested that physalin F was able to maintain protein stability, hence being a good inhibitor candidate for BAFF-R and TACI proteins. The results of this investigation demonstrated substantial promise, indicating that these withanolides and withaphysalin A compounds derived from Physalis angulata offer alternative avenues for B-cell targeting. Consequently, this study presents opportunities for pioneering treatments in the management of nephrotic syndrome.
Co-Authors Adinda Chika Anindita Agustin Iskandar Dicky Faizal Irnandi Edwin Widodo Endharti, Agustina T. Fardizia Putri Alia Hidayat Sujuti Indriati Dwi Rahayu Kardani, Astrid K. Karyono Mintaroem Lavina Sofia Ardani Lindayanti Sumali Loeki E. Fitri M Aris Widodo Mayangsari, Elly Mufidatun Hasanah Nathania Bella Claresta Niarti Ulan Sari Siarnu Nur Permatasari Nura Fattah Cantika Yoga Retty Ratnawati Samsu , Nur Setyawati Soeharto Setyawati Soeharto Subandiyah , Krisni Tika Ardhini Wardoyo Triwahju Astuti Wibowo, Syahputra