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All Journal Microbiology Indonesia Prosiding Seminar Nasional Sains Dan Teknologi Fakultas Teknik Jurnal Ilmu Farmasi dan Farmasi Klinik (Journal of Pharmaceutical Science and Clinical Pharmacy) INDONESIAN JOURNAL OF PHARMACY PHARMACY: Jurnal Farmasi Indonesia (Pharmaceutical Journal of Indonesia) Jurnal Inovasi Teknik Kimia Mediagro Media Farmasi Indonesia

Maulita Cut Nuria

Universitas Wahid Hasyim

Author-ID : 334988

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Engineering Environmental Science Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Other

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Articles

Title

Antibacterial Activities From Jangkang (Homalocladium platycladum (F. Muell) Bailey) Leaves Maulita Cut Nuria
MEDIAGRO Vol 6, No 2 (2010)
Publisher : Universitas Wahid Hasyim

Jangkang (Homalocladium platycladum (F. Muell) Bailey) is ashrub under the Polygonaceae family and grows throughout Indonesia. Thisplant has been traditionally used to relief pain, to cure skin infection and hasantiinflamatory activity. Phytochemical studies showed that this speciescontains saponin, flavonoid and tannin. The aim of this research is to findout antibacterial activities of several extract from jangkang leaves againstGram positive bacteria (Staphylococcus aureus dan Bacillus subtilis) andGram negative bacteria (Escherichia coli dan Salmonella typhi).The dried ground leaves (300.04 gram) were macerated withpetroleum ether, chloroform and methanol respectively. Petroleum ether,chloroform and methanol extracts were obtained after evaporation of thesolvents. The extracts tested for their antibacterial activities using agardiffusion method aided by paper disks in order to find out the inhibitory areadiameters.The result showed that petroleum ether extract did not inhibited thegrowth of bacteria but chloroform and metanol extracts show antibacterialactivity except at E. coli. Chloroform extract shows inhibition zone againstbacteria at concentration of 1000 μg/disk while methanol extract atconcentration of 500 μg/disk.Keywords : Jangkang (Homalocladium platycladum (F. Muell) Bailey)leaves, Antibacterial activity

UJI AKTIVITAS ANTIBAKTERI EKSTRAK ETANOL DAUN JARAK PAGAR (Jatropha curcas L) TERHADAP BAKTERI Staphylococcus aureus ATCC 25923, Escherichia coli ATCC 25922, Dan Salmonella typhi ATCC 1408 Maulita Cut Nuria; Arvin Faizatun; Sumantri .
MEDIAGRO Vol 5, No 2 (2009)
Publisher : Universitas Wahid Hasyim

Jatropha curcas L is a plant species that can be used for curing, since it contains flavonoid, saponin, and tanin. Jatropha multifida L is proved to have antibacterial activities (Sisunandar, et.al., 2002). This research was intended to find out the antibacterial activities of ethanol extracts of Jatropha curcas L leaves on bacteria Staphylococus aureus, Escherichia coli, and Salmonella typhi, find out the effectiveness of those antibacterial activities and identify the compound groups contained in the ethanol extracts, the result of antibacterial activity of ethanol extracts of Jatropha curcas L was analized by statistic. Employing a maceration method, Jatropha curcas L leaves were extracted using ethanol 70% for five days. Employing an agar-diffusion method aided by disk papers and in order to find out the inhibitory area diameters of the extracts, the extracts were then tested for their antibacterial activities. Qualitative analysis on the chemical contents of the extracts were conducted employing. TLC method aided by silica-gel stationary phases for saponin and tanin and cellulose phases for flavonoid. The motion phases used ethyl acetat-acidformiat-acid acetat-water for flavonoid, chloroform-methanol for saponin and buthanol-acid acetat-water for tanin. The result of the test on antibacterial activities were analyzed statistically using Kruskall Wallis and Mann & Whitney tests. The analysis showed that ethanol extracts of the Jatropha curcas L leaves inhibited the growth of Staphylococcus aureus but not the growth of Eschericia coli and Salmonella typhi. The testing concentrations of 20%, 40%, 60%, 80%, and 100% b/v produed inhibitory area diameters averaging 8.25, 9.25, 11.00, 13.25, and 19.00 mm respectively. The positive control of Ampicillin produced an average inhibitory area diameters of 40.00 mm while the solvent control did not produce any inhibitory area diameters. Statistical test showed that there were significant differences among the different concentrations of the extracts.Thin Layer Chromatography tests produced yellow color showing the existence of flavonoid, bluish violet color showing the existence of saponin, and grayish green color showing the existence of tanin. Keyword : ethanol extracts of Jatropha curcas L leaves, inhibitory area diameters, Staphylococcus aureus, Escherichia coli, and Salmonella typhi

Antibacterial Activities of Ethyl Acetate Fraction of Methanol Extract From Sosor Bebek Leaves (Kalanchoe pinnata Pers.) Maulita Cut Nuria; Enny Puji Astuti; Sumantri Sumantri
MEDIAGRO Vol 6, No 2 (2010)
Publisher : Universitas Wahid Hasyim

Kalanchoe pinnata Pers. is one of the famous herbal in Indonesiathat has been used traditionally to treat diarrhea and dysentery. Akinsulire etal., (2007) proved the antibacterial effects of metanolic extract fromKalanchoe pinnata Pers. leaves, and its sensitive to Gram positive andnegative bacteria. Aims of this research are to find out antibacterialactivities of ethyl acetate fraction of methanolic extract from Kalanchoepinnata Pers. leaves to Gram positive bacteria (Staphylococcus aureus,Bacillus subtilis) and Gram negative bacteria (Pseudomonas aeruginosa,Escherichia coli, Salmonella typhi), to determine Minimum InhibitoryConcentration (MIC) of the fraction, and to find out the active chemicalsubstance of the fraction.Methanolic extract of Kalanchoe pinnata Pers. leaves was made byusing maceration method, then the extract was partitioned gradually byusing hexane, chloroform, diethyl ether and ethyl acetate. Ethyl acetatefraction was examinated in vitro according to turbidimetry methods.Concentrations of the fraction were 256, 192, 160, 128, 96, 64, 32 and 16mg/ml. The active chemical substance of the fraction was identified by ThinLayer Chromatography (TLC) method.The result of this research shows the antibacterial activities of ethylacetate fraction of methanolic extract from Kalanchoe pinnata Pers. leavesboth to Gram positive bacteria and Gram negative bacteria. MIC value ofthe fraction to Gram positive bacteria (Staphylococcus aureus, Bacillussubtilis) is 192 mg/ml, and Gram negative bacteria which are Escherichiacoli ATCC 25922 and Pseudomonas aeruginosa is 192 mg/ml, but the MICvalue to Salmonella typhi is 128 mg/ml. TLC identification show that thefraction contains flavonoid.Keywords : Ethyl acetate fraction of methanolic extract from Kalanchoepinnata Pers. leaves, Antibacterial activity, MinimumInhibitory Concentration (MIC), Flavonoid

UJI KANDUNGAN BAKTERI Escherichia coli PADA AIR MINUM ISI ULANG DARI DEPOT AIR MINUM ISI ULANG DI KABUPATEN REMBANG Testing of Escherichia Coli Bacteria Content in Drinking Water Refill from Drinking Water Refill Depot in Rembang Sub-district Maulita Cut Nuria; Abdur Rosyid; Sumantri Sumantri
MEDIAGRO Vol 5, No 1 (2009)
Publisher : Universitas Wahid Hasyim

Water refill is water that has through purification processes both Ultraviolet and ionization, by many stages of filtration to obtain clean water, in order to provide human needs. This research performed by Athena, et.al., (2003) to shows that Total Coli and Escherichia coli in high-enough amounts inside water refill from water refill depot (DAMIU) in Jakarta, Tangerang, and Bekasi. There are many efforts related with this water refill that causes DAMIU developed rapidly, so that required monitoring improvement because water is the primary needs of human being. This research aims to identify Escherichia coli bacteria content that exist in drinking water of water refill from DAMIU in Rembang Sub district. This research is survey type, equipped with microbiology test using MPN method (Most Probable Number). The populations are 25 DAMIU in Rembang sub-district. Data analysis performed descriptively. Test, conducted by water refill sampling, which produced by DAMIU in Rembang sub-district. The testing includes: approximation test using Lactosa Broth (LB) medium, confirmation test using Briliant Green Lactose Bile Broth (BGLB) medium, complementary test using Mc. Conkey medium and Gram painting was performed to identify bacteria types using microscope by 100 times magnification, and Biochemistry test that is IMVIC (Indol, Methyl-Red, Voges Proskauer, and Citrat). In water refill which produced by DAMIU in Rembang Sub district, there is 1 sample (4%) with MPN value of E. coli 13/100 ml and Coliform MPN 21/100 ml, does not fulfill the bacteriology requirements of drinking water quality, according to Health Minister Decree No.907/MENKES/SK/VII/2002 : ³The existence of E.Coli bacteria 0/100 ml sample´, while 24 samples has fulfill the requirements, with MPN value of E.Coli and Coliform < 2, so that it safe and ready to consume. Keywords: Water refill Depot (DAMIU), Most Probable Number (MPN), Escherichia coli

Co-Authors Abdur Rosyid Agung Endro Nugroho Agung Endro Nugroho Aminuyati Amprih Martha, Amprih Anita Dwi Puspitasari Aqnes Budiarti aqnes tuning dyah a Arum Rafiqah, Jovita Arvin Faizatun Asep Gana Suganda Asri Melati Dewi AULIA EVERESTINA PUJAKA dedeh ratna dewi Elin Yulinah Enny Puji Astuti Erika Indah Safitri Fahrina Rachmawati Ikke Safitri mohammad djatmiko Muhamad Insanu Niken Puspitasari Nurahmah Hidayati Puji Astuti Puji Astuti Ratna Asmah Susidarti Ririn Lispita Wulandari risha fillah fithria septaningsih septaningsih Siska Maila Sari Sri Susilowati Sulasmi Sulasmi Sumantri ) Sumantri . Sumantri Sumantri Sumantri Sumantri Sumantri Sumantri Syahar Banu Wahyono . Yance Anas yeni lutfiani Yunita Midkha Zumrotul Chabibah

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