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All Journal Pharmaciana: Jurnal Kefarmasian Indonesian Journal of Cancer Chemoprevention Indonesian Journal of Biotechnology PHARMACY: Jurnal Farmasi Indonesia (Pharmaceutical Journal of Indonesia) JFIOnline Jurnal Farmasi Sains dan Praktis Jurnal Kefarmasian Indonesia
Nunuk Aries Nurulita
Fakultas Farmasi Universitas Muhammadiyah Purwokerto
Biochemistry, Genetics & Molecular Biology Chemistry Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Public Health

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The potentiation effect of Bawang Dayak (Sisyrinchium palmifolium L.) extract on T47D cell growth inhibition after 5-fluorouracil treatment Yuniarti, Azizah; Sundhani, Elza; Nurulita, Nunuk Aries
Pharmaciana Vol. 8 No. 2 (2018): Pharmaciana
Publisher : Universitas Ahmad Dahlan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.12928/pharmaciana.v8i2.9480

Abstract

5-Fluorouracil (5-FU) is chemotherapy agents that used in breast cancer treatment that has toxic effects such as myelotoxicity. its effectiveness relatively low. Bawang Dayak has been widely empirically used as a breast cancer treatment. This study's aims were to determine the sensitivity of 5-FU against T47D cells, as single and its combination with EBD, to determine apoptotic induction, and the inhibitory effect of T47D cell cycle. The cytotoxic assay was using the MTT method with the concetration of 5-FU (0-600μg / mL) and EBD (0-250μg / mL). Apoptotic induction analysis were determined by PI/annexin V. Both of apoptotic and cell cycle analysis was performed by flowcytometry. The results of the 5-FU and EBD cytotoxic test on T47D cells obtained IC50 274μg/mL and 75μg/mL, respectively. The combination of 5-FU and EBD has a mild to strong synergistic effect (CI 0.829 and 0.779). its ombined treatment induced apoptosis in T47D cells by 21.33%. Combination of these combinations caused cell accumulation in G0-G1 phase of 57.11%, greater than control (41.20%). Provision of 5-FU and EBD combination treatments causes disturbance and damage to cells that cause cell cycle arrest in the G0-G1 phase and can not proceed to the next phase. The combination of 5-fluorouracil and EBD has a synergistic effect as breast cancer co-chemotherapy is able to induce apoptosis against T47D cells by triggering cell accumulation in G0-G1 phase. The extract of Bawang Dayak has a high potential to be developed as co-chemotherapy in breast cancer.
Co-Authors . Sugiyanto Agus Siswanto Anjar Mahardian Kusuma Arif Wirahadi Kusuma, Arif Wirahadi Binar Asrining Dhiani Cindy Caesaria, Cindy Darsini Darsini Della Caya Nur Syarifah Dwi Hartanti Edy Meiyanto Edy Meiyanto Edy Meiyanto Eishou Matsuda Elza Sundhani Ika Puspitasari Imam Purnomo Sugiarto, Imam Purnomo Lita Ratriyana Zumrohani Masashi Kawaichi Nastiti, Nindita Sari Novyananda Salmasfattah Septiadi, Binaripan Sharas Swara Maulita, Sharas Swara Solehah, Senja Nur Sugiyanto Sugiyanto Sundhani, Elza Susanti Susanti Tjiptasurasa Tjiptasurasa, Tjiptasurasa Veby Tri Yulianti Weny Delvia Yuniarti, Azizah