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EFEK SITOTOKSIK DAN ANTIPROLIFERATIF EKSTRAK KLOROFORM BUAH MAHKOTA DEWA TERHADAP SEL KANKER PAYUDARA T47D Nurulita, Nunuk Aries; Siswanto, Agus
JFIOnline | Print ISSN 1412-1107 | e-ISSN 2355-696X Vol 3, No 4 (2007)
Publisher : Indonesian Research Gateway

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Abstract

The study on anticancer activity of P. macrocarpa fruit extract had been done. This study was aimed to know cytotoxic and antiproliferative activity of P. macrocarpa Chloroform extract to T47D cell. Cytotoxic and antiproliferative assay of it was done by MTT method. The doubling time and AgNORs staining were used to identify proliferative inhibition of it. Apoptosis of T47D phenomenon wa identified by doubling time method with ethidium bromide-acridyne orange. The result showed than LC50 of P. macrocarpa fruit extract was 103,03 μg/ml. it has antiproliferative effect and prolong doubling time. It also induced apoptosis of T47D cell.   ABSTRAK Telah dilakukan penelitian mengenai efek antikanker ekstrak buah mahkota dewa terhadap sel kanker payudara. Penelitian ini bertujuan untuk mengetahui efek sitotoksik, dan antiproliferasi ekstrak kloroform P. macrocarpa pada sel T47D.  Uji sitotoksik dan antiproliferatif ekstrak kloroform buah mahkota dewa dilakukan dengan metode MTT. Untuk melihat penghambatan proliferasinya dilakukan dengan metode doubling time dan pengecatan AgNORs. Fenomena apoptosis sel T47D yang diberi perlakuan ekstrak kloroform P. macrocarpa dilihat dengan metode double stainning dengan etidium bromida-akridin oranye. Hasil penelitian menunjukkan nilai LC50  sebesar 103,03 μg/ml. ekstrak ini mempunyai efek antiproliferasi dan mampu memperpanjang waktu doubling time. Pengamatan apoptosis dengan double staining menggunakan etidium bromida-akridin oranye, menunjukkan dapat memacu apoptosis sel T47D.
OPTIMASI TABLET FLOATING TEOFILIN MENGGUNAKAN METODE DESAIN FAKTORIAL siswanto, agus; Hapsari, Indri; zulaikha, yusni ulfiani; rosmawati, eva
Farmasains : Jurnal Ilmiah Ilmu Kefarmasian Vol 3 No 1 (2016)
Publisher : Universitas Muhammadiyah Prof. DR. HAMKA

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Abstract

ABSTRACTTheophylline is a drug used in the management of asthma. It has relatively short half-life and narrow therapeutic index with 5-20 μg/mL serum concentration. Thus, Floating tablet formulations that can increase the bioavailability of the drug. The aims of this study were to determine the effect of the excipient (HPMC K4M CR and NaHCO3) to the quality of tablets and determine the optimum formula of theophylline floating tablet. A 22 randomized factorial design was used in development of theophylline floating tablet with HPMC K4M CR (A) and NaHCO3 (B) as independent variables.The flowability of mass tablet, floating character, and dissolution profile were chosen as dependent variables.The results showed that NaHCO3 was the most important factor on increasing flowability of mass tablet. HPMC K4M CR was the dominant factor in lowering the Flag time, improving integrity of the tablet, and decreasing the release of drug. The composition of HPMC K4M CR 40.00 mg and NaHCO3 35.39 mg produced the optimum formula of theophylline floating tablet with desirability value of 0.978.
EFEK SITOTOKSIK DAN ANTIPROLIFERATIF EKSTRAK KLOROFORM BUAH MAHKOTA DEWA TERHADAP SEL KANKER PAYUDARA T47D Nurulita, Nunuk Aries; Siswanto, Agus
Jurnal Farmasi Indonesia Vol 3, No 4 (2007)
Publisher : Jurnal Farmasi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35617/jfi.v3i4.87

Abstract

The study on anticancer activity of P. macrocarpa fruit extract had been done. This study was aimed to know cytotoxic and antiproliferative activity of P. macrocarpa Chloroform extract to T47D cell. Cytotoxic and antiproliferative assay of it was done by MTT method. The doubling time and AgNORs staining were used to identify proliferative inhibition of it. Apoptosis of T47D phenomenon wa identified by doubling time method with ethidium bromide-acridyne orange. The result showed than LC50 of P. macrocarpa fruit extract was 103,03 μg/ml. it has antiproliferative effect and prolong doubling time. It also induced apoptosis of T47D cell.   ABSTRAK Telah dilakukan penelitian mengenai efek antikanker ekstrak buah mahkota dewa terhadap sel kanker payudara. Penelitian ini bertujuan untuk mengetahui efek sitotoksik, dan antiproliferasi ekstrak kloroform P. macrocarpa pada sel T47D.  Uji sitotoksik dan antiproliferatif ekstrak kloroform buah mahkota dewa dilakukan dengan metode MTT. Untuk melihat penghambatan proliferasinya dilakukan dengan metode doubling time dan pengecatan AgNORs. Fenomena apoptosis sel T47D yang diberi perlakuan ekstrak kloroform P. macrocarpa dilihat dengan metode double stainning dengan etidium bromida-akridin oranye. Hasil penelitian menunjukkan nilai LC50  sebesar 103,03 μg/ml. ekstrak ini mempunyai efek antiproliferasi dan mampu memperpanjang waktu doubling time. Pengamatan apoptosis dengan double staining menggunakan etidium bromida-akridin oranye, menunjukkan dapat memacu apoptosis sel T47D.
OPTIMIZATION OF FLOATING TABLET CONTAINING ASPIRIN USING SIMPLEX LATTICE DESIGN Siswanto, Agus
Indonesian Journal of Pharmacy Vol 31 No 1, 2020 In Press
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14499/indonesianjpharm31iss1pp%p

Abstract

Aspirin is an effective analgesic which is also used as an antiplatelet therapy for stroke prophylaxis. Aspirin in floating tablet formulations is used to improve the bioavailability. The aim of this study was to determine the optimum composition of floating tablets of aspirin by simplex lattice design (Design Expert 7.1.5). The tablets were prepared by direct compression technique, using polimers (Methocel K4M CR, Ethocel) and sodium bicarbonate (gas-generating agent) as independent variables. The optimum formula was determined based on numeric method with flow rate of tablet mass, floating lag time, C60, and Td as independent variables. The results indicated that the optimum composition of the matrix was Methocel K4M CR 16.94 %, NaHCO3 15.18 %, and Ethocel 17.88 %. It was observed that independent factors had significant contribution on all dependent variables.
Pemodelan Farmakokinetika Tablet Floating Aspirin pada Kelinci dengan PKSolver AGUS SISWANTO; ACHMAD FUDHOLI; AKHMAD KHARIS NUGROHO; SUDIBYO MARTONO
JURNAL ILMU KEFARMASIAN INDONESIA Vol 14 No 2 (2016): JIFI
Publisher : Fakultas Farmasi Universitas Pancasila

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Abstract

Aspirin selain sebagai analgetik juga efektif digunakan sebagai antiplatelet untuk profilaksis stroke. Aspirin diabsorpsi secara cepat di saluran pencernaan bagian atas terutama di bagian pertama usus halus. Formulasi dalam bentuk tablet floating diharapkan mampu memperbaiki profil farmakokinetika aspirin. Tujuan penelitian ini adalah untuk menentukan model farmakokinetika tablet floating aspirin pada kelinci dengan PKSolver. Penentuan model dengan PKSolver berdasarkan parameter: 1) visual goodness of fit (GOF) dan 2) parameter statistik yang meliputi Akaike’s information criteron (AIC) dan Schwarz criterion (SC). Berdasarkan kedua parameter tersebut menunjukkan bahwa model multiple site absorption (MAS) merupakan model terbaik untuk menjelaskan farmakokinetika tablet floating aspirin. Pelepasan dan absorpsi aspirin dari tablet floating terjadi melalui 2 tahap sehingga menghasilkan profil farmakokinetika dengan puncak ganda akibat absorpsi yang terjadi secara paralel di dalam lambung.
OPTIMASI TABLET FLOATING TEOFILIN MENGGUNAKAN METODE DESAIN FAKTORIAL agus siswanto; Indri Hapsari; yusni ulfiani zulaikha; eva rosmawati
Farmasains : Jurnal Ilmiah Ilmu Kefarmasian Vol. 3 No. 1 (2016)
Publisher : Universitas Muhammadiyah Prof. DR. HAMKA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (280.05 KB)

Abstract

ABSTRACTTheophylline is a drug used in the management of asthma. It has relatively short half-life and narrow therapeutic index with 5-20 μg/mL serum concentration. Thus, Floating tablet formulations that can increase the bioavailability of the drug. The aims of this study were to determine the effect of the excipient (HPMC K4M CR and NaHCO3) to the quality of tablets and determine the optimum formula of theophylline floating tablet. A 22 randomized factorial design was used in development of theophylline floating tablet with HPMC K4M CR (A) and NaHCO3 (B) as independent variables.The flowability of mass tablet, floating character, and dissolution profile were chosen as dependent variables.The results showed that NaHCO3 was the most important factor on increasing flowability of mass tablet. HPMC K4M CR was the dominant factor in lowering the Flag time, improving integrity of the tablet, and decreasing the release of drug. The composition of HPMC K4M CR 40.00 mg and NaHCO3 35.39 mg produced the optimum formula of theophylline floating tablet with desirability value of 0.978.
Pemodelan Pelepasan In Vitro Tablet Floating Teofilin Dengan Program Ddsolver Agus Siswanto; Indri Hapsari; Yusni Ulfiani Zulaikha; Eva Rosmawati
PHARMACY: Jurnal Farmasi Indonesia (Pharmaceutical Journal of Indonesia) Jurnal Pharmacy, Vol. 13 No. 01 Juli 2016
Publisher : Pharmacy Faculty, Universitas Muhammadiyah Purwokerto

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Abstract

ABSTRAK Disolusi merupakan elemen penting dalam pengembangan obat terutama terkait dengan absorpsi dan bioavailabilitas sediaan. Penelitian ini bertujuan untuk menentukan kinetika pelepasan tablet floating teofilin secara in vitro. Tablet floating teofilin dibuat dengan metode granulasi basah menggunakan bahan tambahan HPMC K4M CR, NaHCO3, laktosa, dan magnesium stearat. Hasil evaluasi sifat fisik menunjukkan bahwa tablet floating teofilin mempunyai bobot yang seragam (CV=1,93%), kekerasan yang baik (8,4 kg), dan kerapuhan yang rendah (0,05%). Evaluasi model pelepasan dengan program DDSolver menggunakan 2 kriteria: 1). Parameter statistik: R2adjusted, mean square error (MSE), weighted sum of squares (WSS), akaike information criterion (AIC), model selection criterion (MSC); 2). Visual goodness of fit (GOF). Hasil evaluasi data disolusi dengan program DDSolver menunjukkan bahwa pelepasan tablet floating teofilin mengikuti persamaan Weibull. Kurva disolusi berbentuk eksponensial dan pelepasan teofilin terjadi melalui mekanisme difusi. Kata kunci: tablet floating teofilin, DDSolver, pemodelan pelepasan obat. ABSTRACT The dissolution is an important element in drug development is mainly related to the absorption and bioavailability. The aim of this study was to determine in vitro dissolution study of theophylline floating tablet release kinetics model. The floating tablets were prepared by a wet granulation method with HPMC K4M CR, NaHCO3, lactose, and magnesium stearate as excipients. The result showed that the theophylline floating tablets had a weight uniformity (CV=1.93%), good hardness (8.4 kg), and low friability (0.05%). Dissolution data were evaluated using DDSolver conducted by 1). Statistical parameters: R2adjusted, mean square error (MSE), weighted sum of squares (WSS), akaike information criterion (AIC), model selection criterion (MSC); 2). Visual goodness of fit (GOF). The results showed that theophylline floating tablets release kinetics followed the Weibull equation. The dissolution curve is exponential shape and theophylline release occurs through a diffusion mechanism. Key words: floating tablet of theophylline, DDSolver, modeling of drug release.
OPTIMASI PENGGUNAAN SPRAY DRIED LACTOSE DAN AVICEL PH 102 SEBAGAI FILLER-BINDERS TABLET ASPIRIN Rizki Okprastowo; Agus Siswanto; Suparman Suparman
PHARMACY: Jurnal Farmasi Indonesia (Pharmaceutical Journal of Indonesia) Jurnal Pharmacy, Vol. 08 No. 03 Desember 2011
Publisher : Pharmacy Faculty, Universitas Muhammadiyah Purwokerto

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30595/pji.v8i03.1129

Abstract

ABSTRAK Aspirin merupakan bahan obat yang dapat dibuat dengan metode kempa langsung, karena sifatnya yang tidak tahan terhadap pemanasan dan memiliki sifat alir yang baik. Penelitian ini bertujuan untuk mengetahui pengaruh penggunaan filler-binders Spray Dried Lactose dan Avicel PH 102 terhadap sifat fisik tablet Aspirin dan kombinasi antara kedua bahan tersebut menghasilkan sifat fisik tablet yang optimum. Alasan penggunaan Spray Dried Lactose dan Avicel PH 102 dikarenakan kedua bahan tersebut memiliki sifat alir dan kompresibilitas yang baik serta kombinasi keduanya bertujuan untuk mengurangi biaya formulasi.Tablet Aspirin dibuat dengan metode kempa langsung, terdiri dari 3 formula yang divariasikan pada penggunaan filler-binders. Formula I menggunakan 100% Spray Dried Lactose, formula II menggunakan 100% Avicel PH 102 dan formula III menggunakan kombinasi 50% Spray Dried Lactose dan 50% Avicel PH 102. Parameter yang digunakan adalah sifat fisik yang meliputi sifat alir, kekerasan, kerapuhan dan waktu hancur. Hasil dari penelitian ini menunjukan bahwa penggunaan Spray Dried Lactose dan Avicel PH 102 sebagai filler-binders pada tablet Aspirin berpengaruh positif terhadap waktu alir granul, keseragaman bobot dan kekerasan tablet sedangkan untuk kerapuhan dan waktu hancur perlu disempurnakan lagi pada formulasinya. Berdasarkan metode analisis simplex lattice design dapat diperoleh daerah optimum dari parameter sifat fisik pada tablet Aspirin. Kata kunci: aspirin, filler-binders, laktosa, Avicel ph 102, simplex lattice design ABSTRACT Aspirin is a medicine substance that can made by direct compression, because the characteristic is not good for warmth and have a good fluidity. This research aimed to know the influence of used filler-binders Spray Dried Lactose and Avicel PH 102 for physical characteristic of Aspirin tablet and combination of both can result the tablet’s optimum physical characteristic. The reason of used Spray Dried Lactose and Avicel PH 102 are because both of that substance have a good fluidity and compressibility and combination of both aiming to reduce formulation cost. Aspirin tablets made by direct compression method, consists of three formulas that varied on the use of filler-binders. Formula I use 100% Spray Dried Lactose, formula II using 100% Avicel PH 102 and formula III using a combination of 50% Spray Dried Lactose and 50% Avicel PH 102. The parameters used are physical characteristic that include fluidity, hardness, friability and disintegration time. The results of these research show that the use of Spray Dried Lactose and Avicel PH 102 as filler-binders on Aspirin tablets provide a positive influence to the fluidity of granules, the uniformity of tablet weight and hardness, while for the friability and disintegration time needs to be refined again on the formulation. Based on the simplex lattice design analysis method can be obtained optimum area from the parameters on physical characteristic of Aspirin tablets. Key words: aspirin, filler-binders, spray dried lactose, avicel ph 102, simplex lattice design
Perancangan Sistem Informasi Penitipan Hewan Peliharaan Pada RYOPETSHOP Berbasis Android Prayogo, Lito; Siswanto, Agus; Rahim, Abdul
Jurnal Manajemen Teknologi Dan Sistem Informasi (JMS) Vol 4 No 1 (2024): JMS Vol 4 No 1 Maret 2024
Publisher : LPPM STIKOM Dinamika Bangsa

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33998/jms.2024.4.1.1471

Abstract

This android-based pet hotel information system is essential for RYO PetShop in Jambi. As a pet hotel facility, the current system is still using manual book keeping and operates as a single-user system, resulting in less effective and efficient performance. The pet hotel owner must manage data manually, leading to a higher risk of recording errors or lost information. To address these issues, the pet hotel information system is developed using Java programming language and Firebase, employing the Waterfall method and the unified model language system approach, which includes Use Case diagrams, Activity Diagrams, and class diagrams.
Pembangunan Jaringan Distribusi Air Bersih bagi Masyarakat Desa Sonawan, Hery; Hermawan, M. Reza; Sofia, R. Evi; Rohadin, Rohadin; Prihastuty, Endang; Yudisworo, W. Djoko; Subiyanta, Erfan; Siswanto, Agus; Tohasan, Achmad; Wasiran, Wasiran; Heri, Junial
Berdikari: Jurnal Inovasi dan Penerapan Ipteks Vol 12, No 1: February 2024
Publisher : Universitas Muhammadiyah Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18196/berdikari.v12i1.20276

Abstract

The community service activities in Kaduela Village, which were part of a matching fund program for the year 2023, were executed from July–December 2023 in accordance with the proposed activity plan. The objective of this initiative was to establish a comprehensive water pipeline infrastructure to provide clean water to the inhabitants of Bina Loka and Bina Bakti Hamlet, who lacked access to the numerous springs located in Kaduela Village. Proper and comprehensive piping planning was necessary to provide equitable distribution of water among community members in the two elevated hamlets relative to the water source. The process of addressing issues in Kaduela Village involved several sequential steps. These steps encompassed the collection of primary and secondary data, the depiction of the new piping network on a map of Kaduela Village, the simulation of the new piping network using Epanet software, the construction of the new piping network, and the subsequent commissioning of the system. The chosen pipe network configuration is a dead-end system, which was deemed appropriate for implementation in the Bina Loka and Bina Bakti hamlets, with the aim of providing water services to a total of 58 households in Bina Loka and 15 households in Bina Bakti. The process of pipeline network designing was conducted by Mechanical Engineering students from Universitas Pasundan and Universitas 17 Agustus 1945 Cirebon, under the supervision of their respective supervisors, utilizing the Epanet software. In addition to receiving funding under the 2023 matching fund program, this initiative also garnered comprehensive support from the BUMDES Arya Kamuning as the primary collaborator
Co-Authors Abdul Choliq Hidayat Abdul Manan Abdul Rahim Abdul Rosyid Abidin, Dodo Zaenal Achmad Fudholi ACHMAD FUDHOLI Achmad Fudholi Adithya Wahyu Pratama, Adithya Wahyu Aftoni Sutanto Ahmad Husain Asdie Akhir Riana Handayani, Akhir Riana Akhmad Kharis Nugroho Albait, Nur Aldiansyah, m. Amrullah, Bagaskara Falah Anjar Mahardian Kusuma Arvita, Yulia Ashad, Bayu Adrian Baedhowi, Abdul Latif Baehaqi, Mudofar Beny Dodit Ari Wibowo, Dodit Ari Dwi Hartanti Dwi Rahayuningsih E. Mudjaddid A. Siswanto Deddy N.W.Achadiono Hamzah Shatri Emelia, Shinta Endang Prihastuty, Endang Erfan Subiyanta, Erfan Errissya Rasywir eva rosmawati Eva Rosmawati Evi Sofia Fachruddin Fatimah Malini Lubis Fauziati, Ana Feranika, Ayu Feriyadin, Feriyadin Fiastuti Witjaksono Gustina Nurkhayatun, Gustina Hady, Sultan Hariadi Hariawan Hermawan, M. Reza Herti Yani, Herti Hery Sonawan Hidayat, Abdul Choliq Imam Baihaqi Imam Subekti Indri Hapsari Indri Hapsari Iskandar Soedirman, Iskandar Iskandar Sudirman Jasmir, Jasmir Junial Heri Kuntjoro Harimurti Machrus Ali Megantara, Marcelino A. Moeslich Hasanmihardja Mucharomah, Nurul Maghfiroh MUHAMMAD FURQONI Mutiara Poetri Nurtanti, Mutiara Poetri Nasrun, Martina W. Nunuk Aries Nurulita PAMUSO, MARLIN Paramitha, Cindy Pareza Alam Jusia, Pareza Alam Pebrianto, Feri Prayogo, Lito Pri Iswati Utami Purnomo, Luthfan Budi Rasywir, Errisya Reza Pramitha Habsari, Reza Pramitha Rizana Fauzi, Rizana Rizki Okprastowo rohadin, rohadin Rosalina, Mia Rosario, Maria rosmawati, eva Rosyadi, Marwan RR. Ella Evrita Hestiandari Rudi Putranto Rustiawan, Indra S.Kom.,M.Pd, Arifudin Setiawan, Zunan Siregar, Arief Kurniawan Siti Helmyati Siti Setiati Sofiansyah, Muhammad Edi Sudibyo Martono Sudibyo Martono SUDIBYO MARTONO Suhendro Suwarto, Suhendro Suparman Suparman Suparman Suparman Suryadi, Dikky Sutanto , Aftoni Sutoyo, Mochammad Arief Hermawan Suyanti Suyanti Suyanti Suzanna Immanuel Tohasan, Achmad W. Djoko Yudisworo Wachyu Hari Haji Wardani, Muhammad Wasiran, Wasiran Wibawa, Arief Wibowo, Indi Deli Fiallo Widya Cahya Ariswati, Widya Cahya Yafi, Zidni Reynard Yekti Mulyani, Yekti Yovi Pratama Yuli Asmi Rahman Yuni Ernawati, Yuni yusni ulfiani zulaikha Yusni Ulfiani Zulaikha zulaikha, yusni ulfiani