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All Journal Reaktor Caraka Tani: Journal of Sustainable Agriculture Proceeding Simposium Nasional Kelautan dan Perikanan Jurnal Perikanan Universitas Gadjah Mada Indonesian Food Science and TechnologyJournal Jurnal Sinergitas PkM & CSR FaST : Jurnal Sains dan Teknologi Jurnal Teknologi Hasil Pertanian Food ScienTech Journal Jurnal PkM (Pengabdian kepada Masyarakat) Prosiding Konferensi Nasional PKM-CSR
Lucia Crysanthy Soedirga
Department Of Food Technology, Faculty Of Science And Technology, Universitas Pelita Harapan
Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Computer Science & IT Control & Systems Engineering Earth & Planetary Sciences Economics, Econometrics & Finance Education Energy Environmental Science Immunology & microbiology Materials Science & Nanotechnology Nursing Public Health Social Sciences

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Journal : Reaktor

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N-Acetylglucosamine Production by Repeated-Batch Fermentation Using Immobilized Semi-Purified Chitinase Enzyme on Agar Lucia Soedirga; Hardoko Hardoko; Natasha V Widianto
Reaktor Volume 21 No. 1 March 2021
Publisher : Dept. of Chemical Engineering, Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (301.528 KB) | DOI: 10.14710/reaktor.21.1.11-14

Abstract

Chitinolytic mold, such as Mucor circinelloidesis can be utilized to produce chitinase enzyme for shrimp shell’s chitin hydrolysis into N-acetylglucosamine (NAG). For that purpose, entrapment of chitinase on agar as a carrier could be an alternative way to improve NAG production. This study aimed to investigate the stability of immobilized semi-purified chitinase on agar for multiple cycles fermentation to produce NAG. In this study, 0.6 mL of semi-purified chitinase enzyme was immobilized into 3% of agar matrices and tested for four fermentation cycles to obtain highest NAG concentration and good enzyme activity. The results indicate that the immobilized chitinase could be used for 6 hours fermentation or three fermentation cycles. The NAG concentration produced after three cycle were 1042.22 ± 16.20 ppm. Besides, the immobilized enzyme was considerably stable up to the third cycles with activity value of about 4.74 U/mL.Keywords: agar; immobilized;NAG; repeated fermentation
Physicochemical Characteristics of Butterfly Pea Flower Petals Steep Obtained at Different Steeping Temperature and Time Lucia Soedirga; Intan C Matita; Jessica Sidharta
Reaktor Volume 23 No.1 April 2023
Publisher : Dept. of Chemical Engineering, Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/reaktor.1.1.9-15

Abstract

Flower is an important part of the plant containing phytochemical compounds, especially the phenolic acids, flavonoids, and anthocyanin that can be used as natural antioxidants. Phytochemical compounds are usually drawn by steeping the flower petals in hot water. Indeed, different temperatures and steeping times would affect the yield of phytochemicals obtained. The butterfly pea flower is one of edible flowers that brings antioxidant activity due to the presence of phytochemical compounds, namely flavonoids and phenolic acids. Nevertheless, there have been limited studies dedicated to the search of steeping condition of butterfly pea flower petals in water. Thus, this study aimed to determine the time and temperature of steeping to produce the butterfly pea step with preferred physicochemical characteristics. The result indicates that butterfly pea flower petals which undergo steeping at 60°C for 45 minutes produce antioxidant activity with an IC50 value of 251.89 ± 32.02 ppm. Furthermore, the butterfly pea flower steep produced had blue to purple color with °Hue of 277.06 ±2.55, 1.23 ± 0.07 mg/L of total monomeric anthocyanin content, 6.83 ± 0.02 of pH and 138.42 ± 15.88 mg GAE / g sample of total phenolic content. Keywords: antioxidant; butterfly pea flower; steep; temperature; time
Co-Authors Aileen Widyapranata Anugrahati, Nuri A. Chanan Din, Nabilah binti Christine Joannita Kurniawan Dr. Adolf J.N. Parhusip Hadroko Hadroko Halim, Yuniwaty Handayani, Ratna Hardoko Hardoko Hardoko Hardoko Ilianto, Alvin B. Intan C Matita Intan C Matita Intan Cidarbulan Matita Irene Andriani Jessica Sidharta Joannita Kurniawan, Christine Kam, Natania Kurniawan, Christine J Marchellin Marchellin Matita, Intan C Matita, Intan C. Maurelle Nolita Melanie Cornelia, Melanie Michelle, Valentina Natania Natania Natania, Natania Natasha V Widianto Natasha Vania Widianto Nolita, Maurelle Nuri Arum Anugrahati Richirose Richirose Richirose Richirose Sinaga, Wenny S.L. Suharso, Eveline Tagor M Siregar Tagor M. Siregar Tagor Marsillam Siregar Terezya E. Wijaya Titri S. Mastuti Valentina Michelle Vania Vania Vania Vania Weny Sinaga Wijaya, Terezya E. Wilbur Donald R. Pokatong Yuniwati Halim