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Olan Rahayu Puji Astuti Mussa
Universitas Wijaya Kusuma Surabaya
Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology

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PERBANDINGAN KADAR LEMAK DAN BERAT JENIS SUSU SAPI PERAH Friesian Holstein (FH) DI BENDUL MERISI, SURABAYA (Dataran Rendah) dan NONGKOJAJAR, PASURUAN (Dataran Tinggi) Ruswandono Wirjatmadja; Olan Rahayu Puji Astuti Mussa; Ratna Widyawati; M Dzaki Wiranda Pratama
VITEK : Bidang Kedokteran Hewan Vol 10 (2020): VITEK - Bidang Kedokteran Hewan
Publisher : Fakultas Kedokteran Hewan Universitas Wijaya Kusuma Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30742/jv.v10i0.47

Abstract

This study aims to compare the quality of Friesian Holstein (FH) dairy cows in Bendul Merisi, Surabaya (Lowlands) and Nongkojajar, Pasuruan (Highlands), with the physical quality standard of fresh milk which is the national reference or SNI. This study used 60 samples of cow's milk, 30 samples from Bendul Merisi, and 30 samples from Nongkojajar. The sample was tested in the laboratory using the fat content and specific gravity test, the data obtained were calculated using the T-test formula. The data obtained from the average fat content test in Nongkojajar was 3.3685%, and the average fat content in Bendul Meisi was 4.8003%. The average density at Nongkojajar is 1.024767 mg / dl, the average density at Bendul Merisi is 1.28067 mg / dl. Based on the results of statistical calculations resulted in a significant difference (P <0.01) in the fat content test, statistical calculations resulted in a significant difference (P <0.01) in the specific gravity test.
EFEKTIVITAS SEDIAAN SALEP EKSTRAK BUNGA KECOMBRANG (Etlingera elatior) TERHADAP PENYEMBUHAN LUKA INSISI PADA TIKUS (Rattus norvegicus) Ratna Widyawati; Olan Rahayu Puji Astuti Mussa; Miarsono Sigit; Bernadeta Perwirawati Lepu Geli
VITEK : Bidang Kedokteran Hewan Vol 9 (2019): VITEK - Bidang Kedokteran Hewan
Publisher : Fakultas Kedokteran Hewan Universitas Wijaya Kusuma Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30742/jv.v9i0.48

Abstract

This study was conducted to determine the effect of ointment extract of kecombrang flower (Etlingera elatior) on healing incision wounds in white rats (Ratus norvegicus) in terms of the formation of wound scab, wound closure time and wound attachment. The experimental research used is Randomized complete design with 4 treatments with 6 replications. The treatments applied were P0 as a negative control, P1 (20% kecombrang flower extract ointment), P2 (30% kecombrang flower extract ointment) and P3 (40% kecombrang flower extract ointment). Parameters of this study were the wound caropeng, wound closure time and wound attachment. The results obtained were different median values in the four groups with p = 0,000 (p <0.05) in the Kruskal Wallis test. In the Mann Whitney test were significant differences between the groups of 20% kecombrang flower extract ointment concentration, 30% concentration and 40% concentration in the negative control group. The most effective result is ointment extract of kecombrang flower 20% on healing incision wounds.
GAMBARAN HISTOPATOLOGI PARU PADA MENCIT (Mus musculus) YANG DIPAPAR ASAP ROKOK KRETEK AKIBAT PENGARUH PEMBERIAN INFUSA TEH HIJAU (Camellia sinensis) Dyah Widhowati; Rondius Solfaine; Olan Rahayu Puji Astuti Mussa; Apriska Ayuningtias
VITEK : Bidang Kedokteran Hewan Vol 10 (2020): VITEK - Bidang Kedokteran Hewan
Publisher : Fakultas Kedokteran Hewan Universitas Wijaya Kusuma Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30742/jv.v10i0.53

Abstract

This study was conducted to determine the effect of antioxidant administration from green tea infusion (Camellia sinensis) on damage to the histological structure of the lung exposed to clove cigarette smoke in mice (Mus muscullus). Free radicals in cigarette smoke such as tar, nicotine, and carbon monoxide can cause various damages to the lung or This study was conducted to determine the effect of antioxidant administration from green tea infusion (Camellia sinensis) on damage to the histological structure of the lung exposed to clove cigarette smoke in mice (Mus muscullus). Free radicals in cigarette smoke such as tar, nicotine, and carbon monoxide can cause various damages to the lung organs due to oxidative stress caused by the reaction of ultrafine particles with cells. A total of 27 mice were taken from the Biochemical Laboratory of Airlangga University, Surabaya. In this study mice (Mus muscullus) were exposed to kretek cigarette smoke in the smoking chamber for 15 minutes in a period of 2 weeks, used 2 variants of antioxidant doses of green tea infusion of 0.45 gr to 0.6 gr. After the treatment was completed, the preparation of the mice's pulmonary organ preparations and damage to the structure of the lung organ were observed in 9 visual fields. The results showed that there was a relationship between the amount of cigarette smoke exposed and damage to the lung organs of mice when given antioxidant green tea. The administration of green tea antioxidants reduced inflammation of the lung organ infiltration of inflammatory cells by 44%, edema by 55%, and destruction of septa alveoli by 88%. gans due to oxidative stress caused by the reaction of ultrafine particles with cells. A total of 27 mice were taken from the Biochemical Laboratory of Airlangga University, Surabaya. In this study mice (Mus muscullus) were exposed to kretek cigarette smoke in the smoking chamber for 15 minutes in a period of 2 weeks, used 2 variants of antioxidant doses of green tea infusion of 0.45 gr to 0.6 gr. After the treatment was completed, the preparation of the mice's pulmonary organ preparations and damage to the structure of the lung organ were observed in 9 visual fields. The results showed that there was a relationship between the amount of cigarette smoke exposed and damage to the lung organs of mice when given antioxidant green tea. The administration of green tea antioxidants reduced inflammation of the lung organ infiltration of inflammatory cells by 44%, edema by 55%, and destruction of septa alveoli by 88%.
PERBANDINGAN PAPARAN ASAP ROKOK KONVENSIONAL DAN ROKOK HERBAL PADA MENCIT (Mus musculus) TERHADAP PERBANDINGAN GAMBARAN HISTOLOGI PARU Nurul Hidayah; Olan Rahayu Puji Astuti Mussa; Rondius Solfaine; Yudha Sapto Utomo
VITEK : Bidang Kedokteran Hewan Vol 10 (2020): VITEK - Bidang Kedokteran Hewan
Publisher : Fakultas Kedokteran Hewan Universitas Wijaya Kusuma Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30742/jv.v10i0.55

Abstract

The purpose of this study was to determine the comparison of conventional cigarette smoke and herbal cigarettes against histological damage in the lungs of mice. The research method uses a completely randomized design with 21 mice aged 8-12 weeks and divided into 3 treatment groups. Group P0 was not given exposure to cigarette smoke, group P1 was exposed to conventional cigarette smoke tar content was 39 mg nicotine 2 mg, group P2 was exposed to herbal cigarette smoke tar content was 41.60 mg nicotine 1.64 mg. On the 15th day the mice were deuthanated for pulmonary extraction and haematoxyline eosin staining was made. Data analysis using one way ANOVA. The results of this study obtained scoring lung damage to edema lesions in P0, P1, P2 Sig 0.246 (p> 0.05). Inflammation of inflammatory cells at P0, P1, P2 Sig 0.000 (p <0.05). Destruction of alveolar septum at P0, P1, P2 Sig 0.000 (p <0.05). The conclusion of this study is the difference in lung damage in mice in the form of edema, infiltration of inflammatory cells, destruction of the alveolar septum between exposure to conventional cigarette smoke and herbal cigarettes. The purpose of this study was to determine the comparison of conventional cigarette smoke and herbal cigarettes against histological damage in the lungs of mice. The research method uses a completely randomized design with 21 mice aged 8-12 weeks and divided into 3 treatment groups. Group P0 was not given exposure to cigarette smoke, group P1 was exposed to conventional cigarette smoke tar content was 39 mg nicotine 2 mg, group P2 was exposed to herbal cigarette smoke tar content was 41.60 mg nicotine 1.64 mg. On the 15th day the mice were deuthanated for pulmonary extraction and haematoxyline eosin staining was made. Data analysis using one way ANOVA. The results of this study obtained scoring lung damage to edema lesions in P0, P1, P2 Sig 0.246 (p> 0.05). Inflammation of inflammatory cells at P0, P1, P2 Sig 0.000 (p <0.05). Destruction of alveolar septum at P0, P1, P2 Sig 0.000 (p <0.05). The conclusion of this study is the difference in lung damage in mice in the form of edema, infiltration of inflammatory cells, destruction of the alveolar septum between exposure to conventional cigarette smoke and herbal cigarettes.
PERHITUNGAN TELUR TOXOCARA CATI DAN JUMLAH SEL DARAH PUTIH PADA KUCING LIAR (Felis catus) DI DUKUH KUPANG SURABAYA bagus uda palgunadi; Roeswandono Roeswandono; Olan Rahayu Puji Astuti Mussa; Micco Joshua Apriano Pangaribuan
VITEK : Bidang Kedokteran Hewan Vol 9 (2019): VITEK - Bidang Kedokteran Hewan
Publisher : Fakultas Kedokteran Hewan Universitas Wijaya Kusuma Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30742/jv.v9i0.62

Abstract

The research aimed to know the degree of infection from egg calculated and increased numbers of white blood cells (eosinophils and lymphocytes) in wild cats (Felis catus). The samples of the research as many as 30 wild cats around the Around of Dukuh Kupang Surabaya . The examination was done by Mc Master method with 100x magnification and the calculated of white blood cells (eosinophil and lymphocytes). The results showed the degree of toxocariasis infection in wild cats (Felis catus) in Surabaya in Dukuh Kupang was seen from the average value of the 468.3 egg examination and the calculated of white blood cells (eosinophilic and lymphocytes) with an average value of eosinophilic 0.73 x103/μl and lymphocytes 10.72 x103/μl. This study used descriptive method. Toxocariasis infection was included in the degree of moderate infection in wild cats (Felis catus) in Dukuh Kupang Surabaya with moderate degree.
INFEKSI ENDOPARASIT PADA KUCING DOMESTIK (Felis domesticus) DI PASAR TRADISIONAL KECAMATAN SAWAHAN KOTA SURABAYA Dyah Widhowati; Rochiman Sasmita; Olan Rahayu Puji Astuti Mussa; Helda Asniyani Benu
VITEK : Bidang Kedokteran Hewan Vol 9 (2019): VITEK - Bidang Kedokteran Hewan
Publisher : Fakultas Kedokteran Hewan Universitas Wijaya Kusuma Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30742/jv.v9i0.67

Abstract

Toxoplasma gondii is an intracellular parasite of the protozoan group and is obligate parasite with definitive host is a cat and other family felidae, Toxoplasma gondii can cause Toxoplasmosis and is categorized as one of zoonotic diseases. This study aims to determine the Toxoplasma gondii infection in domestic cats (Felis domesticus) in the traditional markets of Sawahan sub-district, Surabaya City. This study used a faecal sample of 46 domestic cats in the traditional markets of the Sawahan sub-district consisting of 20 female cats, 10 male cats, and 16 kittens. Then examined at the Laboratory of Parasitology, Faculty of Veterinary Medicine, Wijaya Kusuma University. The examination was carried out by the native method using eosin 2% and the floating method using saturated sugar and observed under a microscope with a magnification of 100x-400x. The results showed there were no cats infected with Toxoplasma gondii but found 3 endoparasites namely Ancylostoma sp 23.9%, Toxocara cati 41.3% and Isospora rivolta 4.3%.
Co-Authors Apriska Ayuningtias Bagus Uda Palgunadi, Bagus Uda Bernadeta Perwirawati Lepu Geli Helda Asniyani Benu M Dzaki Wiranda Pratama Miarsono Sigit Micco Joshua Apriano Pangaribuan Nurul Hidayah Ratna Widyawati Ratna Widyawati Rochiman Sasmita Roeswandono Roeswandono Rondius Solfaine Ruswandono Wirjatmadja Widhowati, Dyah Yudha Sapto Utomo