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All Journal Jurnal Ilmu Peternakan dan Veteriner Tropis (Journal of Tropical Animal and Veterinary Sciences) Vitek : Bidang Kedokteran Hewan Jurnal Ilmiah Fillia Cendekia IGKOJEI: Jurnal Pengabdian Masyarakat Prosiding Seminar Nasional Kusuma
Nurul Hidayah
Fakultas Kedokteran Hewan, Universitas Wijaya Kusuma Surabaya
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Economics, Econometrics & Finance Education Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Medicine & Pharmacology Social Sciences Veterinary Other

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Profil Diferensiasi Sel Th-1 pada Penderita Tuberkulosis Nurul Hidayah; Sofia Fajarwati
VITEK : Bidang Kedokteran Hewan Vol 5 (2015): VITEK - Bidang Kedokteran Hewan
Publisher : Fakultas Kedokteran Hewan Universitas Wijaya Kusuma Surabaya

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Abstract

Penyakit tuberkulosis (TB) disebabkan oleh infeksi bakteri Mycobacterium tuberculosis (M. tuberculosisi). Resiko penularan setiap tahun (Annual Risk of Tuberculosis Infection = ARTI) di Indonesia dianggap cukup tinggi dan bervariasi antara 1-2%. Pada daerah dengan ARTI 1%, maka diantara 100.000 penduduk, rata-rata terjadi 100 penderita tuberkulosis setiap tahun, dimana 50% penderita adalah BTA positif. Walaupun vaksin telah ada, namun prevalensi TB belum berkurangMycobacterium tuberculosis terhirup dalam bentuk aerosol droplet nuclei dan mencapai sekmen distant dari bronchoalveolar tree, terutama pada bagian bawah paru-paru. M. tuberculosis difagosit oleh makrofag alveolar. Makrofag ini mempunyai dua fungsi yaitu sebagai efektor antimikroba dan untuk respon sitokin proinflamatori.Sel Th1 berasal dari sel T CD4+. Sel TCD4+ dapat berdiferensiasi menjadi Th1 dan Th2. Pemilihan jalur perkembangan ditentukan oleh sinyal dari sitokin yang diterima. M. tuberculosis merupakan bakteri intraselular. Pengenalan makrofag terhadap M. tuberculosis bisa melalui TLR-2, yang pada akhirnya akan menghasilkan molekul sitokin IL-12. Stimulasi dari sel NK juga didapatkan yaitu berupa IFN-γ. IFN-γ ini juga menstimulasi makrofag untuk menghasilkan IL-12. IL-12 adalah sitokin kunci untuk mengubah Th0 menjadi Th1 dan menginduksi celluler mediated immunity (CMI)CMI sangat dibutuhkan untuk mengeradikasi bakteri. Hal ini dikarenakan antibodi akan sulit menjangkau M. tuberculosis yang sudah berada dalam sel. Oleh karena itu, Th1 yang mengawali induksi CMI lebih diperlukan dari pada Th2. Sehingga berbagai sel dan molekul yang dibutuhkan untuk diferensiasi Th0 menjadi Th1 juga sama pentingnya. LAM adalah salah satu komponen dinding sel M. tuberculosis. Karena berada dipermukaan, LAM sangat mungkin menjadi bagian yang pertama dikenali oleh sistem imun hospes. Dari beberapa penelitian LAM diketahui dapat meningkatkan diferensiasi sel T menjadi Th1.
Pengaruh Pemberian Perasan Kunyit (Curcuma Domestica Val.) terhadap Jumlah Monosit dan Heterofil yang Dipapar Antigen Salmonella Pullorum Ayam Pedaging Komersial Dyah Widhowati; Nurul Hidayah; Reza Febrianto Nugroho
VITEK : Bidang Kedokteran Hewan Vol 5 (2015): VITEK - Bidang Kedokteran Hewan
Publisher : Fakultas Kedokteran Hewan Universitas Wijaya Kusuma Surabaya

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Abstract

Penelitian ini bertujuan untuk mengetahui pengaruh pemberian perasan kunyit terhadap jumlah monosit dan heterofil pada ayam pedaging yang diinfeksi dengan antigen Salmonella pullorum. Hewan percobaan yang digunakan adalah ayam pedaging sejumlah 40 ekor. Rancangan yang digunakan adalah Rancangan Acak Lengkap (RAL) dengan 4 perlakuan dan 5 pengulangan. Keempat perlakuan tersebut adalah P0 (kontrol), P1 (40% perasan kunyit), P2 (50% perasan kunyit), P3 (60% perasan kunyit). Pemberian perasan kunyit dilakukan mulai dari DOC sampai umur 14 hari pada saat pagi hari (maksimal 4 jam pemberian). Infeksi antigen Salmonella pullorum dilakukan pada umur 7 hari. Jumlah monosit dan heterofil dihitung dari hasil preparat ulas darah. Hasil penelitian ini menunjukkan bahwa pemberian perasan kunyit dengan konsentrasi 50% menunjukkan peningkatan jumlah monosit dan heterofil jika dibandingkan dengan kelompok perlakuan 40% dan 60%.
PERBANDINGAN PAPARAN ASAP ROKOK KONVENSIONAL DAN ROKOK HERBAL PADA MENCIT (Mus musculus) TERHADAP PERBANDINGAN GAMBARAN HISTOLOGI PARU Nurul Hidayah; Olan Rahayu Puji Astuti Mussa; Rondius Solfaine; Yudha Sapto Utomo
VITEK : Bidang Kedokteran Hewan Vol 10 (2020): VITEK - Bidang Kedokteran Hewan
Publisher : Fakultas Kedokteran Hewan Universitas Wijaya Kusuma Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30742/jv.v10i0.55

Abstract

The purpose of this study was to determine the comparison of conventional cigarette smoke and herbal cigarettes against histological damage in the lungs of mice. The research method uses a completely randomized design with 21 mice aged 8-12 weeks and divided into 3 treatment groups. Group P0 was not given exposure to cigarette smoke, group P1 was exposed to conventional cigarette smoke tar content was 39 mg nicotine 2 mg, group P2 was exposed to herbal cigarette smoke tar content was 41.60 mg nicotine 1.64 mg. On the 15th day the mice were deuthanated for pulmonary extraction and haematoxyline eosin staining was made. Data analysis using one way ANOVA. The results of this study obtained scoring lung damage to edema lesions in P0, P1, P2 Sig 0.246 (p> 0.05). Inflammation of inflammatory cells at P0, P1, P2 Sig 0.000 (p <0.05). Destruction of alveolar septum at P0, P1, P2 Sig 0.000 (p <0.05). The conclusion of this study is the difference in lung damage in mice in the form of edema, infiltration of inflammatory cells, destruction of the alveolar septum between exposure to conventional cigarette smoke and herbal cigarettes. The purpose of this study was to determine the comparison of conventional cigarette smoke and herbal cigarettes against histological damage in the lungs of mice. The research method uses a completely randomized design with 21 mice aged 8-12 weeks and divided into 3 treatment groups. Group P0 was not given exposure to cigarette smoke, group P1 was exposed to conventional cigarette smoke tar content was 39 mg nicotine 2 mg, group P2 was exposed to herbal cigarette smoke tar content was 41.60 mg nicotine 1.64 mg. On the 15th day the mice were deuthanated for pulmonary extraction and haematoxyline eosin staining was made. Data analysis using one way ANOVA. The results of this study obtained scoring lung damage to edema lesions in P0, P1, P2 Sig 0.246 (p> 0.05). Inflammation of inflammatory cells at P0, P1, P2 Sig 0.000 (p <0.05). Destruction of alveolar septum at P0, P1, P2 Sig 0.000 (p <0.05). The conclusion of this study is the difference in lung damage in mice in the form of edema, infiltration of inflammatory cells, destruction of the alveolar septum between exposure to conventional cigarette smoke and herbal cigarettes.
PENGARUH PEMBERIAN KRIM EKSTRAK BAWANG MERAH (ALLIUM CEPA L.) TERHADAP PROSES PENYEMBUHAN LUKA BAKAR PADA TIKUS PUTIH (Rattus Novergicus) Nurul Hidayah; Asih Rahayu; Adhitya Yoppy Ro Candra; Kurnia Latif
VITEK : Bidang Kedokteran Hewan Vol 9 (2019): VITEK - Bidang Kedokteran Hewan
Publisher : Fakultas Kedokteran Hewan Universitas Wijaya Kusuma Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30742/jv.v9i0.66

Abstract

This study aimed to determine the influence of onion extract cream (Allium cepa) for burning burns in white rats (Rattus norvegicus). The research was experimental with a concentration of 45%, 50%, and 55% onion Cream Extract (Allium cepa). The Data can then be processed using the Kruskall Wallis test. From the statistical test results, each level of Bakart wound showed noticeable difference. The P < 0.05 is thus distinct from P1, P2, and P3. At the level of fluid burns Sig 0.000, because (P < 0.05) There were noticeable difference in the treatment of PO with P1, P2, and P3. The conclusion of this study of the introduction of onion extract Cream (Allium cepa.) can be used for wound healing in white rats (Rattus norvegicus).
Pengaruh ekstrak teh kombucha (Medusomyces gisevii) terhadap berat dan histopatologis limpa tikus wistar (Rattus norvegicus) yang diinfeksi Escherichia coli Nurul Hidayah; Indra Rahmawati; Jessica Amelia; Yos Adi Prakoso
VITEK : Bidang Kedokteran Hewan Vol 12 No 1 (2022): VITEK-Bidang Kedokteran Hewan
Publisher : Fakultas Kedokteran Hewan Universitas Wijaya Kusuma Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30742/jv.v12i1.97

Abstract

Penelitian bertujuan untuk mengetahui pengaruh dari ekstrak teh kombucha (Medusomyces gisevii) terhadap berat dan histopatologis limpa tikus wistar (Rattus norvegicus) yang diinfeksi Escheichia coli. Penelitian menggunakan 24 ekor tikus wistar jantan yang dibagi kedalam 6 kelompok dan 4 kali pengulangan. Tikus diberi ekstrak teh kombucha selama 28 hari setelah 1 minggu pengadaptasian. Kelompok terdiri dari kontrol positif (K+), kontrol negatif (K-), konsentrasi 30% (P1), konsentrasi 40% (P2), konsentrasi 50% (P3), konsentrasi 60% (P4). Kelompok K+, P1, P2, P3, P4 diinfeksi Escherichia coli pada hari ke-36 dengan dosis 106 CFU/ml sebanyak 1 ml secara intraperitoneal. Pengambilan organ limpa dilakukan pada hari ke-40. Analisa data menggunakan Analysis Of Variance(ANOVA) dan Duncan’s Multiple Range Test (DMRT). Hasil penelitian menunjukan tidak ada perbedaan yang nyata antar perlakuan pada berat limpa. Histopatologis limpa menunjukan adanya perbedaan yang sangat nyata antar perlakuan. Hasil tertinggi pada konsentrasi 60% (P4) dengan diameter pulpa putih 440,6um, diameter germinal center 227,3um dan lebar zona marginalis 171,6. Kesimpulan penelitian, ekstrak teh kombucha berpengaruh terhadap berat limpa, diameter pulpa putih, germinal center dan lebar zona marginalis pada dosis 60% dan 40%.
PENDAMPINGAN PEMERIKSAAN HEWAN DAN DAGING QURBAN DI MASA PANDEMI COVID-19: Assistance In The Examination Of Animal And Qurban Meat During The Covid-19 Pandemic Freshinta Jellia Wibisono; Lailia Dwi Kusuma Wardhani; Retina Yunani; Adhitya Yoppy Ro Candra; Asih Rahayu; Bagus Uda Palgunadi; Nurul Hidayah; Desty Apritya; Dyah Widhowati; Era Hari Mudji
IGKOJEI: Jurnal Pengabdian Masyarakat Vol. 3 No. 1 (2022): IGKOJEI: Jurnal Pengabdian Masyarakat
Publisher : Fakultas Peternakan Universitas Papua

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.46549/igkojei.v3i1.264

Abstract

Slaughtering of sacrificial animals is a worship activity for slaughtering ruminant livestock which is carried out on Eid al-Adha and carried out in mosques or outside Slaughterhouses (RPH). The limited facilities affect the welfare of the animals so that it affects the quality of the meat. Supervision in the production chain with antemortem and postmortem inspections is carried out to obtain good quality meat. This community service aims to help the community get a guarantee of safe, healthy, whole and halal, sacrificial animal meat with antemortem and postmortem examinations, as well as holding counseling on the handling of sacrificial animals and meat during the covid-19 pandemic through webinars. The activity was carried out in 13 mosques in the areas of Surabaya, Sidoarjo, Gresik, and Pasuruan. The antemortem examination will be held on 19-22 July 2021 followed by a post-mortem examination which will be held on 20-22 July 2021 with a total number of 112 cows, 220 goats and 1 sheep. The Webinar was held earlier, namely on July 14, 2021, by 3 competent speakers. The webinar was attended by the general public, mosque takmir, lecturers, and students. The results of the antemortem examination indicated that the animals were suitable for slaughter, although 2–6 (0.6–1.8%) animals showed diarrhea, nasal and eye discharge, and infection of the prepuce. The results of postmortem examination stated that the animals were fit for consumption with a note that the organs infected with worms had to be removed, namely 15.2% of the liver of cattle and 0.9% of the liver of goats with fasciolosis, 0.5% of goats experiencing parampistomiasis and 1.7% of cattle experiencing eurytrematosis.
Profil Berat Badan Dan Kadar Protein Itik Alabio (Anas plathycus borneo) Jantan Pasca Pemberian Keong Mas (Pomacea canaliculata) Nurul Hidayah; Muhammad Ezra S.; Desty Apritya
Jurnal Ilmiah Fillia Cendekia Vol 5 No 2 (2020): Jurnal Ilmiah Fillia Cendekia
Publisher : Universitas Islam Kadiri

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32503/fillia.v5i2.1172

Abstract

ABSTRAK Penelitian ini bertujuan mengetahui profil berat badan dan kadar protein itik alabio jantan (Anas plathycus borneo) pasca pemberian Keong Mas (Pomacea canaliculata). Sampel penelitian menggunakan itik Alabio jantan sebanyak 24 ekor. Rancangan penelitian ini terdiri dari 4 perlakuan dan 6 kali ulangan, yaitu P0 (kontrol), P1 (pemberian 5% Keong Mas pada pakan), P2 (pemberian 10% Keong Mas) dan P3 (pemberian 15% Keong Mas). Profil berat badan diperoleh dari penimbangan setiap minggu hingga minggu ke-10. Profil kadar protein diperoleh dengan menggunakan Uji Kjeldal. Data dianalisis menggunakan Uji One Way ANOVA dan dilanjutkan dengan Uji Duncan. Hasil penelitian menunjukkan, pemberian Keong Mas mempengaruhi profil berat badan dan kadar protein. Berat badan pada kelompok P3 yaitu 1156.67 ± 8.165 dengan kadar protein sebesar 65.183 ± 2.6709. Pemberian Keong Mas (Pomacea canaliculata) mempengaruhi berat badan dan kadar protein daging itik Alabio jantan. Semakin banyak pemberian Keong Mas (Pomacea canaliculata), semakin meningkat berat badan dan kadar protein. Kata Kunci : Itik Alabio, Keong Mas, Berat badan, Protein, Uji Kjeldahl.
Potensi Daun Sambiloto (Andrographis paniculata Nees) Sebagai Anthelmitik Ascaridia Galli Pada Ayam Kampung (Gallus domesticus) Nurul Hidayah; Miarsono Sigit; Maria Gabrielis Dua Bura
Jurnal Ilmiah Fillia Cendekia Vol 6 No 1 (2021): Jurnal Ilmiah Fillia Cendekia
Publisher : Universitas Islam Kadiri

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32503/fillia.v6i1.1448

Abstract

This study aimed to determine the anthelmintic potency of sambiloto leaf extract (Andrographis paniculata nees) against Ascaridia galli worms in vitro. The research sample was 125 Ascaridia galli worms obtained from the small intestine of native chickens. The research design used a completely randomized design with 5 treatments and 5 replications, namely the treatment group that was given physiological 0.9% NaCl (P1), the group that was given piperazine citrate (P2), the group that was given 20% concentration of sambiloto extract (P3). were given an extract concentration of 40% (P4) and the group given an extract concentration of 60% (P5). Observation of worm mortality was carried out every hour for 24 hours. The data obtained were analyzed using the One-way ANOVA test and followed by the 5% Duncan test. The results showed the sig. that is p 0.00 <0.05 means that there were significant differences between treatment groups. The 60% concentration of sambiloto leaf extract had high anthelmintic power and was comparable to the positive control (piperazine citrate). The conclusion of the study showed that the extract of sambiloto leaves (Andrographis paniculata nees) had anthelmintic power against Ascaridia galli worms in vitro. Keywords: Ascaridia galli worm, sambiloto leaf extract, anthelmintic
Potensi Ekstrak Daun Pala (Myristica Fragrans Houtt) Sebagai Pengawet Alami Terhadap Kualitas Daging Ayam Broiler Ratna Widyawati; Nurul Hidayah; Wardhani Lailia Dwi Kusuma; Marsela Irnawati Nuwa
Jurnal Ilmiah Fillia Cendekia Vol 5 No 2 (2020): Jurnal Ilmiah Fillia Cendekia
Publisher : Universitas Islam Kadiri

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32503/fillia.v5i2.1170

Abstract

The purpose of this study was to determine the potential of nutmeg leaf extract (Myristica fragrans houtt) on organoleptic (color, smell, texture), pH value, and Total Plate Count (TPC) of broiler chicken meat. The experimental design used in this study was a randomized complete design with 4 treatments and 6 replications namely P0 (control), P1 (nutmeg leaf concentration 20%), P2 (nutmeg leaf concentration 30%), P3 (nutmeg leaf concentration 40%) on broiler chicken meat that was marinated for 30 minutes and allowed to stand for 24 hours. Data of organoleptic test used Kruskal Wallis analysis. P3 increased the color of the meat with an average ranking of 90,00, suppressed the smell of meat with an average ranking of 104,50, and maintained the texture of the meat with an average ranking of 82,05. The pH and Total Plate Count (TPC) tests used statistical analysis of the Analysis of Variants. The average pH value at P1 was 6,6917, P2 was 6,3750, P3 was 5,9250. The average Total Plate Count (TPC) at P1 was 5,1×103, P2 was 1,1×103, P3 was 0,6×103. Statistical analysis showed that nutmeg leaf extract significantly affected organoleptic, pH value, and Total Plate Count (TPC) of broiler chicken meat (P<0,05). The conclusion of this study was that the concentration of 40% has the most potential for organoleptic, pH values ​​and Total Plate Count (TPC).
Kasus Ornithobacterium rhinotracheale dari Sampel Trachea pada Beberapa Jenis Unggas Reina Puspita Rahmaniar; Nurul Hidayah; Dyah Widhowati
Jurnal Ilmiah Fillia Cendekia Vol 6 No 1 (2021): Jurnal Ilmiah Fillia Cendekia
Publisher : Universitas Islam Kadiri

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32503/fillia.v6i1.1473

Abstract

The aim of this study was to report cases of respiratory tract infection caused by the bacterium Ornithobacterium rhinotracheale. Observations included anatomical pathology and bacterial isolation results. Poultry was necropsed, observed changes in organs, trachea samples were taken aseptically then isolated on Blood Agar, Chocholate Agar, MCA, microscopic examination and biochemical tests, including TSIA, SIM, SCA, Catalase, Urease, MR, VP. The results of the examination showed the presence of Ornithobacterium rhinotracheale bacteria, the results showed small, grayish colored colonies. Microscopic examination, gram-negative pleomorphic rod-shaped bacteria. Bacteria did not grow on MCA, there was no change in TSIA media, non-motile bacteria, the results of the indole test, SCA, MR and urease were negative. All four types of birds showed pathological changes, including hemorrhage in the trachea and lungs, but the air sacs were normal and did not show any specific lesions, only duck samples with code I-11 had changes in the air sacs. Based on the results of isolation and bacteria, it can detect the presence of Ornithobacterium rhinotracheale from several different types of poultry, including layer chickens, broilers, local ornamental chickens and ducks.
Co-Authors Adhitya Yoppy Ro Candra Asih Rahayu Bagus Uda Palgunadi, Bagus Uda Desty Apritya Era Hari Mudji Indra Rahmawati Jessica Amelia Kurnia Latif Lailia Dwi Kusuma Wardhani Lailia Dwi Kusuma Wardhani Maria Gabrielis Dua Bura Marsela Irnawati Nuwa Miarsono Sigit Muhammad Ezra S. Olan Rahayu Puji Astuti Mussa Rahmaniar, Reina Puspita Ratna Widyawati Reina Puspita R Reza Febrianto Nugroho Rondius Solfaine Sofia Fajarwati Wardhani Lailia Dwi Kusuma Widhowati, Dyah Yos Adi Prakoso Yudha Sapto Utomo Yunani, Retina