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All Journal Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Jurnal Kesehatan Reproduksi Indonesian Journal of Biotechnology The Indonesian Biomedical Journal Jurnal Gizi Klinik Indonesia Jurnal Kesehatan Reproduksi Berita Kedokteran Masyarakat Medical and Health Journal
Heru Pradjatmo
Department Of Obstetrics And Gynecology, Faculty Of Medicine, Public Health, And Nursing, Universitas Gadjah Mada/Dr. Sardjito Hospital, Jl. Farmako, Sekip Utara, Yogyakarta 55281
Biochemistry, Genetics & Molecular Biology Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Nursing Public Health

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Journal : Indonesian Journal of Biotechnology

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MicroRNA-21 as a biomarker for ovarian cancer detection Aprilia Indra Kartika; Siti Nur Chasanah; Akbar Satria Fitriawan; Dewi Sahfitri Tanjung; Addin Trirahmanto; Heru Pradjatmo; Teguh Aryandono; Sofia Mubarika Haryana
Indonesian Journal of Biotechnology Vol 23, No 1 (2018)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (120.369 KB) | DOI: 10.22146/ijbiotech.35692

Abstract

Ovarian cancer is a lethal disease. One of the problems faced by patients with ovarian cancer is the lack of symptoms in its early stages, which results in it only being detected when it is at an advanced stage. Therefore, there is an urgent need for biomarkers that can predict ovarian cancer precisely. The purpose of this study was to determine the expression of microRNA-21 as a predictive biomarker candidate in both early- and advanced-stage ovarian cancer. This was a cross-sectional study using the blood plasma of 21 healthy control subjects and 37 blood plasma samples from patients with ovarian cancer. Blood plasmas were collected, from which the RNA was isolated. Based on the RNA, the cDNA was synthesized and run through qPCR, the results of which were analyzed using the Livak method. The results showed an upregulation of microRNA-21 in the advanced stage by 2.14 fold compared with the early stage, and 6.13 fold compared with the healthy controls (p < 0.05). The upregulation of microRNA-21 in early-stage ovarian cancer was 2.86 fold compared with the healthy control subjects (p < 0.05). In addition, there was an increase in the expression of microRNA-21 in ovarian cancer by 4.14 fold compared with the healthy controls (p < 0.05). Based on these results, it can be concluded that the expression of microRNA 21 upregulated with the severity of the disease.
Co-Authors Addin Trirahmanto Adelina Amelia Adelina Amelia Adintyo Rahman, Muhammad Nur Afif Fuadi Ahsanuddin Attamimi Ahsanudin Attamimi Akbar Satria Fitriawan Alkaff, Muhammad Lutfi Aprilia Indra Kartika Aprivinta, Sherlyana Mega Ardhanu Kusumanto Ardhanu Kusumanto Attamimi, Ahsanuddin Bob Irsan Bob Irsan Choery Novitasari Dewi Sahfitri Tanjung Deyna Primavita Pahlevi Diah Rumekti Hadiati Djaswadi Dasuki Djaswadi Dasuki Djaswadi Dasuki Emilia Theresia Eny Fatmawati Ery Kus Dwianingsih Fatmawati, Eny Geni, Puti Lenggo H.R. Siswosudarmo Hari Kusnanto Ifrinda Giantari Irianiwati Widodo Irsan, Bob Irwan Taufiqur Rachman Ksyatria, Yudhistya Ngudi Insan Laili Chilmawati Laili Chilmawati Mardiana Mardiana Mardiana Mardiana Moh Nailul Fahmi Mohammad Hakimi Muhammad Lutfi Muhammad Lutfi Muhammad Nur Adintyo Rahman Novitasari, Choery Nuratul Awaliyah NURDIANSYAH, FARID Nurul Hikmah Petrana Ova Emilia Pahlevi, Deyna Primavita Petrana, Nurul Hikmah Puti Lenggo Geni putra, prayuna Rifqi, Rifqi Rukmono Siswishanto Shinta Prawitasari Shinta Prawitasari Siti Nur Chasanah Siti Sholikhah Sofia Mubarika Haryana Sumawan, Herman Syamsul Arifin Teguh Aryandono Tri Gunawan Tri Gunawan, Tri Yudhistya Ngudi Insan Ksyatria