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All Journal JURNAL TEKNOLOGI LINGKUNGAN Media Penelitian dan Pengembangan Kesehatan Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Buletin Kebun Raya BERITA BIOLOGI Jurnal Penelitian Pembelajaran Fisika MAGNA MEDICA: Berkala Ilmiah Kedokteran dan Kesehatan Teknologi Indonesia Molekul: Jurnal Ilmiah Kimia Berita Biologi

Praptiwi Praptiwi

Puslit Biologi LIPI, Jl. Raya Jakarta-Bogor Km. 46 Cibinong 16911 Indonesia

Author-ID : 344846

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Computer Science & IT Decision Sciences, Operations Research & Management Environmental Science Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Physics Public Health

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SKRINING BEBERAPA JAMUR ENDOFIT TUMBUHAN DARI PULAU ENGGANO, BENGKULU SEBAGAI ANTIBAKTERI DAN ANTIOKSIDAN [Screening of Plant Endophytic Fungi from Enggano Island, Bengkulu for Antibacterial and Antioxidant Activites] wulansari, dewi; Putra, Aldho Pramana; Ilyas, Muhammad; Praptiwi, Praptiwi; Fathoni, Ahmad; Palupi, Kartika Dyah; Agusta, Andria
BERITA BIOLOGI Vol 15, No 3 (2016)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Fungal endophytes have been known as sources for bioactives with high chemical structure variability. This study aimed to screen extracts of some endophytic fungi associated with plants from Enggano Island as antioxidant and antibacterial using thin layer chromatography (TLC) bioautography method. Antibacterial ativity was performed against Escherichia coli and Staphylococcus aureus. Seventeen out of 22 extracts showed growth inhibition activity against both bacteria, while 16 extracts were active as DPPH free radical scavenging agents. Further determination on IC50 value of four prominent extracts revealed that fungal endophytes AK3018-1, FC-1, KC-4, and SO-3 have IC50 value of 85, 84, 704, and 347 ?g/ml, respectively in DPPH radical scavenging method. Extracts of fungal endophytes CR-3, CS-2, and SM-2 showed prominent antibacterial activity among other extracts, indicated by wide clear white zone around the spot. Further evaluation on minimum inhibitory concentration (MIC) of those three extracts by microdilution method showed that CR-3, CS-2, and SM-2 have MICs value of 512, >512, and 64Âµg/ml, respectively against S. aureus.Fungal endophytes AK3018-1, FC-1, KC-4, SO-3, CR-3, CS-2, and SM-2 were respectively isolated from Dioscorea bulbifera tuber, Fibraurea chloroleuca twig, Knema cinerea twig, Smilax odoratissima stem, Cryptocarya sp. twig, Calophyllum soulattri twig, and Smilaxmacrophylla stem.

KOMPONEN KIMIA DAN UJIANTIBAKTERIMINYAK ATSIRIDAUN KI CENGKEH (Urophyllum arboreum (Reinw. ex. Bl.) Korth.) Praptiwi, Praptiwi; Jamal, Yuliasri; Murningsih, Tri
BERITA BIOLOGI Vol 6, No 3 (2002)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

This study was done to determine the chemical compounds and antibacterial activity of ki cengkeh (Urophyllum arboreum (Reinw.ex. Bl.) Korth.) essential oil. Ki cengkeh has already known as medicinal plant in some places in Indonesia. This might be correlated with its chemical compounds. The analysis of chemical compounds of ki cengkeh essential oil was done by GC-MS, while antibacterial test was done by paper disc method against 3 bacteria isolates (Staphylococcus aureus, S. epidermidis and Eschericia coli). The result of GC-MS analysis showed that there were 25 compounds with the concentration more than 1% and carryophylen oxide as the highest compound (12.03%). The highest antibacterial activity was found against S. epidermidis.

PENAPISAN FITOKIMA DAN BILANGAN PEROKSIDA (POV) TIGA JENIS TUMBUHAN FAMILI MORACEACE (Artocarpus sp., A.elasticus, dan Ficus sp.) DARI TAMAN NASIONAL BOGANI NANI WARTABONE (SULUT) Harapini, Mindarti; Praptiwi, Praptiwi
BERITA BIOLOGI Vol 6, No 4 (2003)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

The Â aim of study were to determine the chemical compounds and peroxide values (POV) of methanol extract of three plants species belongs to Moraceae family (Artocarpus (Artocarpus sp., A.elasticus, dan Ficus sp.). Their chemical compounds were identified by Cuiley method, while peroxide value was done by iodometri titration. Methanol extract percentage ofÂ Artocarpus sp., A.elasticus, dan Ficus sp. were 20.80%, 3.10% and 4.10% respectively. All of the extract tested contained essential oil, sterol and triterpenoid, aglicon flavonoid, tannin, flavonoid glicoside and saponin. POV ofÂ Artocarpus sp., A.elasticus, dan Ficus sp. were 325.24, 306.25 and 1580.07 respectively and POV of q tocopherol was 363.96. So, it can be concluded taht all of three methanol extract act as reductor, because their POV less that POV of q tocopherol and might be act antioxidant. These correalted with their chemical compounds such as flavonoid, tannin and peroxide sugar.

TOKSISITAS AKUT ORAL DUA SENYAWA BISANTRAKUINON (+)-2,2â€™-EPISITOSKIRIN A DAN (+)-1,1â€™-BISLUNATIN [ORAL ACUTE TOXICITY OF TWO BISANTHRAQUINONES (+)-2,2â€™-EPICYTOSKYRIN A AND (+)-1,1â€™-BISLUNATIN] Praptiwi, Praptiwi; Nurkanto, Arif; Wulansari, Dewi; Agusta, Andria
BERITA BIOLOGI Vol 14, No 1 (2015)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v14i1.1858

Bisanthraquinones (+) - 2,2'-epicytoskyrin A and (+) -1,1'bislunatin produced by the endophytic fungus Diaporthe sp. GNBP-10 showed potent antibacterial activity on in-vitro test and have the opportunity to become new antibiotics candidates. The aspects of safety and toxicity of drug candidates have to be examined before applying to human. This study was conducted to determine the safety aspects of the compounds through acute oral toxicity testing in mice (Mus musculus). Acute toxicity of (+) - 2,2'-epicytoskyrin A and (+) - 1,1'-bislunatin evaluated by the method of Up and Down Procedure with limit test at a dose of 2000 mg / kg. Results of acute toxicity test showed that the LD50 of (+) - 2,2'-epicytoskyrin A and (+) - 1,1'-bislunatin were of 1638.87 mg / kg and > 2000 mg / kg respectively. Administration of (+)- 2,2'-epicytoskyrin A resulted in increased miliari multifocal hepatitis, fatty degeneration and necrosis of liver cells, and the renal tubule epithelial degeneration. Administration of (+) - 1,1'-bislunatin at a dose of 2000 mg / kg resulted in multifocal accumulation of inflammatory cells in the liver and degeneration of cells in the islets of Langerhans although not resulting in death. The administration of those compounds indicated the changes in the organs, but based on the UN/ECE classification of LD50 value showed that (+) 2,2'- epicytoskyrin A and (+) -1,1'-bislunatin included as low acute toxicity substance.

SKRINING AWAL AKTIVITAS ANTIBAKTERI DAN ANTIOKSIDAN EKSTRAK SEMUT (INSECTA: FORMICIDAE) DARI GARUT - JAWA BARAT Efendy, Oscar; Fathoni, Ahmad; Praptiwi, Praptiwi; Royyani, Mohammad Fathi; Wulansari, Dewi; Agusta, Andria
BERITA BIOLOGI Vol 18, No 2 (2019)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v18i2.3621

Studies on the therapeutic use of insects and insect products have been neglected compared to the use of other animals or plants.This study aims to determine the antibacterial and antioxidant potential of ants extracts. Preliminary study related to antibacterial and antioxidant screeningof 17 extracts of ant colonies that belongs to 8 species were performed by Thin Layer Chromatography (TLC)-Bioautography. Antioxidant activity was measured by DPPH free radical scavenging method. The antibacterial activity was done against three pathogenic bacteria, i.e Bacillus subtilis InaCC B-1, Staphylococcus aureus InaCC B-4 and Escherichia coli InaCC B-5 were performed by non-eluted TLC-autobiography assay. The minimum inhibitory concentration (MIC) and IC50 values of DPPH radical scavenging activity of active extracts were determined by microdilution in 96-well microplate. The results showed 6 extracts active against B.subtilis, 5 extracts active against S.aureus. The lowest MIC value was 512 µg/ ml. Ten extracts had the antioxidant activity with various IC50 values. The extracts of ants might be used as bioactive resources for antibacterial and antioxidant.

Co-Authors Ahmad Fathoni ANDRIA AGUSTA Arif Nurkanto Arti, Dwi Windu Kinanti Arti, Dwi Windu Kinanti Chairunnisa Chairunnisa Dewi Ratih Agungpriyono DEWI WULANSARI Dwinna Rahmi Efendi, Sintia Frisky Efendy, Oscar Emma Sri Kuncari Hafid, Lukman Hartutiningsih-M. Siregar Jeffry Handhika Kamal, Andi Saptaji Kartika Dyah Palupi lina marlina, lina Mindarti Harapini Mohammad Fathi Royyani, Mohammad Fathi Muhammad Ilyas Priyono, Sumarnie Putra, Aldho Pramana Putri, Ade Lia R. Subekti Purwantoro SUDARMONO SUDARMONO Suryati Suryati Tri Murningsih, Tri Yuliasri Jamal

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