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KULTUR POLIEMBRIONI BIJI DUKU (Lansium domesticum. CORR.) PADA MEDIA MS DAN WPM DENGAN PENAMBAHAN AIR KELAPA Prihatini, Retno; Yahya, Sudirman; Purwito, Agus
Sainstek : Jurnal Sains dan Teknologi Vol 2, No 1 (2010)
Publisher : IAIN Batusangkar

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (928.736 KB) | DOI: 10.31958/js.v2i1.4

Abstract

The study on growth respons of Lansium domesticum  poliembryoni seed on MS and WPM media with added by coconut water had been carried out from Maret up to Mei 2006. This experiment had been done in Tissue Culture Laboratory, Agronomy-Holticulture Depatment, Agronomy Faculty, Institute Pertanian Bogor, using  two factorial of Completely Randomized Design with  nine treatments, and ten replications. The first factor treatment were  MS, ½ MS and WPM media, and the 2nd  factor were 0, 15 and 20% coconut water. The result showed that the emergence time of the first adventitious shoot were beginning on 26,5 days after planting, with one until four numerous of shoots, on MS, ½ MS, WPM media with added  until 20% coconut water. There were only 50-70% shoot formed on 8 weeks after planting. With 20% coconut water suplemented on MS culture media showed that the height of shoots were significant than controle.Key words: Lansium domesticum, coconut water, polyembryonic seed, culture media. 
Morphology Character and Andrographolide Quantifications on Sambiloto  ( Andrographis paniculata (Burm.F.) Nees) Retno Prihatini; Auzar Syarif; Amri Bakhtiar
Bioscience Vol 4, No 1 (2020): Biology
Publisher : UNIVERSITAS NEGERI PADANG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/0202041107669-0-00

Abstract

ABSTRACTThe Morphology character and andrographolide quantification on Sambiloto (A. Paniculata) that growth in Sawah Dangka Jorong Gaduik, Tilatang Kamang, Agam (S 00o16159.311 E100o23110.311)  have been studied.  The Morphology character  of  A. Paniculata on generative fase  have been done in the field and Laboratorium of Biology Department, Andalas University. Quantification of  andrographolide constituen were analysed by using High Performance Liquid Chromatography (HPLC) in Chemical of Nature Resources Laboratorium, Pharmacy Faculty, Andalas University. The result showed that Morphology character of  Sambiloto ( A. Paniculata ) include parameters  ie. range of height plant ( 27-45 cm) ; range of nodus numerous ( 10-14 ); range of leaf-7 size (length; width)  ( 3.8-4.1 ; 0.8-1.1) cm; range of branch numerous ( 8-10), leaf dry weight rate  (2.482 g ), stem dry weight rate  ( 5.882 g ), and day old flowering rate  ( 110 day ). The Result of quantification secondary metabolite with high economic value, namely andrographolide showed respectively with level  2.208% (vegetative) and  2.780 % (generative).  Key Words : Andrographis paniculata, morpology chracter, andrographolide quantification  ABSTRAKTelah dilakukan studi tentang Karakter morfologi dan kuantifikasi senyawa andrografolide pada tanaman Sambiloto (A. Paniculata) yang hidup di Sawah Dangka Jorong Gaduik, Tilatang Kamang, Agam (S 00o16159.311 E100o23110.311). Karakter morfologi   A. Paniculata pada fase generative telah dilakukan di lapangan dan di  Laboratorium di Jurusan Biologi Universitas Andalas. Kuantifikasi senyawa andrografolid dianalisa dengan menggunakan High Performance Liquid Chromatography (HPLC) di Laboratorium Kimia Bahan Alam, Fakultas Farmasi Universitas Andalas.  Hasil menunjukkan bahwa karakter morfologi Sambiloto    ( A. Paniculata ) meliputi parameter  yaitu kisaran tinggi tanaman ( 27-45 cm) ; kisaran jumlah nodus ( 10-14 ); kisaran ukuran daunke-7  (panjang; lebar)  ( 3,8-4,1 ; 0,8-1,1) cm; kisaran jumlah cabang ( 8-10), rata-rata berat kering daun  (2,482 g ), rata-rata berat kering batang ( 5,882 g ), and rata-rata umur berbunga  ( 110 day ).  Hasil kuantifikasi metabolit sekunder dengan nilai ekonomi tinggi, yang dikenal sebagai senyawa andrografolid menunjukkan level   2,208%  (vegetatif) dan 2,780 % (generatif). Kata kunci: Andrographis paniculata, karakter morfologi, kuantifikasi andrografolid. 
PEMANFAATAN AIR KELAPA UNTUK MENINGKATKAN PERTUMBUHAN AKAR STEK TUNAS AKSILAR Andrographis paniculata Nees Retno Prihatini
EKSAKTA: Berkala Ilmiah Bidang MIPA Vol. 18 No. 02 (2017): Eksakta : Berkala Ilmiah Bidang MIPA (E-ISSN : 2549-7464)
Publisher : Faculty of Mathematics and Natural Sciences (FMIPA), Universitas Negeri Padang, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (137.449 KB) | DOI: 10.24036/eksakta/vol18-iss02/54

Abstract

The research has been conducted to find out the best concentration of coconut water in the induction of roots of cuttings axillary buds Andrographis paniculata Nees. This research was conducted in Plant Physilogy of Division, Department of Biology, Faculty of Math and Natural Science, AndalasUniversity, for 12 weeks. The research used Completely Randomized Design. The material used was axillary buds A. paniculata with nodal number 3. treated for 30 minutes in coconut water 10, 15 and 20% and aquadest as a controle each with 6 replications. Observations were done on live percentage, root length, root numerous and cuttings height after 12 weeks planting. The results showed that the concentration 10 to 15% of coconut water was able with significant to induce the root growth of cutting axillary buds A. Paniculat. The concentration 15% of coconut water was the best in inducing cutting height growth, each with an average root length is 8.2 cm and the average number of roots that formed is 16, 0 and the average cutting height is 22.7 cm. There was an increase of average of cuttings height growth by 86% which was supported by the average increase of root length and root number of 105.0% and 280.9% on the occasion of cuttings of axillary buds in coconut water concentration 15%.
Inventarisasi Tumbuhan yang Berpotensi Penghasil Minyak Atsiri dari Famili Lamiaceae di Sumatera Barat Berbasis Spesimen Herbarium Ratih Wulandari; Nurainas Nurainas; Aadrean Aadrean; Syamsuardi Syamsuardi; Retno Prihatini
Jurnal Biologi Universitas Andalas Vol 11, No 2 (2023)
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jbioua.11.2.62-69.2023

Abstract

Lamiaceae is an important plant family that inhabits various ecosystems and has great diversity, is considered a cosmopolitan plant and has an important role in pharmaceutical and cosmetic sciences because of its essential oil content in almost every species. This research was focused on identify plant species from the Lamiacecae family that potential as essential oil producer in West Sumatra with their distribution based on ANDA Herbarium specimens. Data was collected use the herbarium method and literature study to determine the potential of each species. The results showed in the ANDA Herbarium were 45 species from 20 genera, which dominated by species that have the potential to produce essential oils as many as 23 species, followed by species that had not explore for their essential oil content as 11 species and commercial species,  9 species. Species from most Lamiaceae family were potentially as essential oil producer found in Padang city administrative area. Keywords: Cosmopolitan, genera, commercial, distribution, potential
King of Bitter (Andrographis paniculata Nees) Somatic Embryogeni Callus Culture on MS Medium With Added by 2,4-D and BAP Prihatini, Retno; K.Huda, Nadyatul; Idris, Muhamad
Bioscience Vol 8, No 2 (2024): Biology
Publisher : UNIVERSITAS NEGERI PADANG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/bsc.v8i2.130950

Abstract

In this research we have studied about somatic embryogenesis derived on Sambiloto (Andrographis paniculata) cv. VGT callus culture by addition BAP (benzylamino purine) and 2,4-D (2,4 diclorophenoxyacetic acid)   on MS (Murashige and Skoog) Medium. King of Bitter seeds were germinated on MS medium for 3 weeks, then  the second leaf  that full opened used as explants. The explant planting  on medium with several concentration growth regulator (BAP combinated with 2,4-D) to callus induce.  MS medium  with added by 0.5 ppm BAP+ 0.5 ppm 2,4-D  ( A treatment), 0.5. ppm BAP + 1 ppm 2,4-D (B treatment ), 0.5 ppm BAP + 1.5 ppm 2,4-D (C treatment ), 0.5 ppm BAP + 2 ppm 2,4-D (D treatment), each of treatment with 5 replications. The observation used descriptive method for callus texture, somatic embryo derived with it development fase  after subcultured on MS medium with the same concrentration BAP  and 2,4-D . The result showed that callus  have formed on  all of treatment (A, B,C and D), whereas callus with somatic embryogenesis only on C treatment, with the range of callus fresh weight (FW) were 0.6-1.0 g.  After 3 weeks at second subcultured on MS medium with added 0.5 ppm BAP+ 1,5 ppm 2,4-D ,  somatic embryo  development showed  globular fase  and heart shape fase .
Optimizing The User Interface of Waste Bank Application Using UCD and UEQ Prihatini, Retno; Rianto
Jurnal Nasional Pendidikan Teknik Informatika : JANAPATI Vol. 13 No. 3 (2024)
Publisher : Prodi Pendidikan Teknik Informatika Universitas Pendidikan Ganesha

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23887/janapati.v13i3.83998

Abstract

Environmental cleanliness is an essential aspect of life to make a healthy and comfortable environment. In Indonesia, the volume of waste will reach 70 million tons by 2022, with around 24% or 16 million tons needing to be appropriately managed. Related to the significant waste growth, the Ministry of Environment has developed the Waste Bank initiative, a collaborative effort that aims to educate the public in sorting waste and raising awareness of the importance of wise waste management. The desire of the local environmental agency to connect with the community supports the researcher in developing the Waste Bank application. The application will implement an optimal User Interface (UI) and User Experience (UX) design. The User-Centered Design (UCD) method will be employed, supported by the User Experience Questionnaire (UEQ), and is used to design UI and UX for the Waste Bank mobile application. The application prototypes were tested and evaluated using UEQ. The first design achieved an average score but still required improvement. In contrast, the second design scored excellently in six aspects measured: attractiveness, perspicuity, efficiency, dependability, stimulation, and novelty, with significant improvement. These results show that the UCD and UEQ methods are effective for developing UI/UX designs to meet user needs and can be applied in mobile application developments.
Pengembangan sistem keamanan laser untuk melindungi aset museum dari pencurian dan manipulasi Prihatini, Retno; Wijayanto, Ristu Aji; Nurjaman, Muhammad; Sahria, Yoga
Informasi Interaktif : Jurnal Informatika dan Teknologi Informasi Vol 9 No 1 (2024): JII Volume 9, Number 1, Januari 2024
Publisher : Program Studi Informatika Fakultas Teknik Universitas Janabadra

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37159/jii.v9i1.73

Abstract

Museum memiliki peran penting dalam pelestarian pengetahuan seputar sejarah, budaya, dan seni. Menurut statistik dari Kementrian Pendidikan, Kebudayaan, Riset, dan Teknologi (Kemendikbud Ristek), pada tahun 2020, Indonesia telah memiliki 439 museum dan akan bertambah seiring berjalannya waktu. Namun, museum rentan terhadap kejahatan seperti pencurian, kerusakan, dan manipulasi aset. Dengan berkembangnya teknologi, khususnya Internet of Things (IoT), memberikan solusi dalam pengelolaan aset museum. Penelitian ini menggunakan metode Test Driven Development (TDD), yang merupakan proses pengembangan perangkat lunak yang menekankan pada penulisan tes sebelum menulis kode sebenarnya. Aplikasi Android dibuat untuk mengevaluasi serta menganalisis keefektifan system deteksi berbasis laser. Hasil perancangan telah berhasil dan pengujian pada perangkat IoT terbukti berhasil. Pada pengujian perangkat Android dengan menggunakan LCOV Converage menunjukkan angka presentase mencapai 86.7% dari 100%, sehingga menandakan keberhasilan pengujian. Harapannya, penelitian ini dapat memberikan wawasan mendalam mengenai pemanfaatan Internet of Things (IoT) dan kontribusinya dalam meningkatkan keamanan koleksi museum, baik di Indonesia maupun di seluruh dunia.