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All Journal Jurnal Ilmu Ternak Veteriner BERITA BIOLOGI Animal Production : Indonesian Journal of Animal Production
B Purwantara
Departemen Klinik, Reproduksi dan Patologi, Fakultas Kedokteran Hewan, Institut Pertanian Bogor
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Veterinary

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Effect of isoflavone-enriched soybean flour, zinc (Zn) and vitamin E in the ration on testosterone level and total permatogenic cell in seminiferous tubules of rat ., Astuti; Muchtadi, D; Astawan, M; Purwantara, B; Wresdiyati, T
Indonesian Journal of Animal and Veterinary Sciences Vol 13, No 4 (2008)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (246.724 KB) | DOI: 10.14334/jitv.v13i4.573

Abstract

The objective of this experiment are to evaluate the effects of isoflavoneen-enriched soybean flour, zinc (Zn) and vitamin E on testosterone level of male rats and total spermatogenic cells in the seminiferous tubules of rat testes as animal model. Diet was given as isonitrogen and isocaloric with 15% of dietary protein. Thirty male Sprague Dawley rats were divided into six groups and treated with isoflavoneen-riched soybean flour, Zn and vitamin E in different combination. Isoflavoneen-riched soybean flour (3mg/day) was given by oral administration, whereas Zn and vitamin E were mixed wih the basic diet. The treatment was done for 2 month. Results indicated that complete treatment of isoflavoneen-riched soybean flour, Zn and vitamin E on male rats increased testosteron level and total spermatogenic cells in comparison with single treatment and the other combination. The best results showed in the group that given isoflavoneen-enriched soybean flour with diet containing both Zn and vitamin E i.e; testosteron level 3.49 ± 0.31 ng/ml; while the number of spermatogonia, spermatocytes, early spermatids, late spermatids, and total spermatogenic cells were 37.56 ± 4.48, 67 ± 4.72, 287.11 ± 31.75, 227.22 ± 29.78, and 618.89 ± 47.38, respectively. It was concluded that synergic interaction between isoflavoneen-enriched soybean flour, Zn and vitamin E increased testosteron level and total spermatogenic cells of rat testes.       Key Words: Isoflavone-Riched Soybean Flour, Zn, Vitamin E, Testosterone, Spermatogenic Cells
The viability of fresh and extended semen of stallion with different sperm concentration in Dimitropoulos-modified extender ., Yudi; Arifiantini, I; Purwantara, B; Yusuf, T.L
Indonesian Journal of Animal and Veterinary Sciences Vol 13, No 1 (2008)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (80.697 KB) | DOI: 10.14334/jitv.v13i1.593

Abstract

The objective of the experiment was to study the motility and viability of spermatozoa of fresh semen, and the quality of extended semen with different sperm concentration in Dimitropoulos-modified extender. Semen was collected using artificial vagina from three 4-8 year old stallions (different breed). Semen characteristics and quality was evaluated macro- and microscopically. For longevity evaluation, semen was stored at room and chilled temperature, and was evaluated for motility and viability every 3 h. Prior extension, semen was centrifugated at 3000 rpm for 20 minutes. The condensed sperm was re-suspended in Dimitropoulos (DV) supplemented with 50 mM fructose with the concentration of 200, 100 and 50 x 106 spz/mL. All samples were stored at room and chilled temperature, and was evaluated for motility and viability every 3 h and 12 h for room and chilled temperature. Results of the experiments indicated that fresh semen characteristics was fairly good. For longevity evaluation, semen with motility of 48.33 and 10.42% was observed at 3 h and 12 h after the onset of storage. The extended-semen with 50 x 106 spz/mL showed significantly higher in term of motility and viability (P<.05) than that with 200 x 106 spz/mL, but for that of 100 x 106 spz/mL. It is recommended that sperm concentration should be 50 x 106 spz/ml for a long period storage with reasonable good quality. Key words: Stallion, Semen, Sperm Concentration, Dimitropoulos
Integrity of swamp buffalo sperm on a variety of semen freezing process ., Herdis; Purwantara, B; Supriatna, I; Putu, I.G
Indonesian Journal of Animal and Veterinary Sciences Vol 4, No 1 (1999)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (156.795 KB) | DOI: 10.14334/jitv.v4i1.131

Abstract

Sperm of swamp buffalo bulls is easily damaged during freezing process. Acrosomal intact and plasma membrane intact is important factors in fertilization process. This experiment was aimed to study the effect of freezing method on sperm integrity. The result of experiment indicated that the mean of intact acrosomal and the intact plasma membrane for 4 hours of equilibration (52.24 ± 3.70% and 54.34 ± 4.80%) was significant higher (P<0.05) than 2 hours of equilibration (39.00 ± 3.32% and 43.44 ± 4.91%) but was not significantly difference (P>0.05) with 6 hours of equilibration (47.92 ± 4.51% and 51.58 ± 4.25%). There were not significance difference between one step and two step of glycerolization. The best sperm integrity was resulted by freezing method with 4 hours of equilibration and two steps glicerolization.   Key words : Swamp buffalo bulls, sperm integrity, freezing process
The Viability of the Stallion Spermatozoa after Centrifugation with Different Level of Seminal Plasma in Skim Milk Extender Arifiantini, RI; Yusuf, T Laswardi; Purwantara, B
ANIMAL PRODUCTION Vol 8, No 3 (2006): September
Publisher : Universitas Jenderal Soedirman, Faculty of Animal Science, Purwokerto-Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (159.339 KB)

Abstract

The objective of the research was to evaluate the speed and duration of centrifugation and seminal plasma levels on the viability of stallion spermatozoa.   Semen was collected from three stallions twice a week. The collected semen then evaluated macro- and microscopically.  Exp I, the semen was diluted 1:1 with skim milk extender and then centrifuged at 2000 and 3000 rpm for 15 and 20 minutes each. The seminal plasma was removed, and the sperm pellet was re-diluted with skim milk extender. The extended semen then divided in two tubes, stored in 5oC and in a room temperature.  The semen then was examined every 3 hours for room temperature and 12 hours for 5oC.  Exp II, the semen diluted 1:1 with skim milk extender and then centrifuged at 3000 rpm for 15 minutes. The seminal plasma was removed and the pellet was re-diluted with skim milk extender consisted of 0%, 25%, 50% and 75% of the seminal plasma.  The extended semen then was stored in 5oC and examined daily.  Results showed that speed and duration of centrifugation had no effect on the quality of stallion semen. The liquid semen without seminal plasma showed 47.50% motile sperms and 61.00% life sperms, wich were significantly higher (P<0.05) than those with seminal plasma. It was concluded that the speed and duration of centrifugation had no effect on the semen quality and removal of seminal plasma is beneficial for stallion spermatozoa. (Animal Production 8(3): 160-167 (2006) Key Words : Centrifugation, stallion semen and seminal plasma
Optimalization of Equilibration and Thawing Methode on Freezing Process of Garut Ram Semen (Ovis aries) Herdis, Herdis; Toelihere, MR; Supriatna, I; Purwantara, B; Adikara, RTS
ANIMAL PRODUCTION Vol 7, No 2 (2005): May
Publisher : Universitas Jenderal Soedirman, Faculty of Animal Science, Purwokerto-Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (126.047 KB)

Abstract

Generally, the problem of semen freezing process  is  cold shock effect and intracelullar change due to water release which is  related to  ice crystal formation. One factor to solve the problem is finding out optimal equilibration time and thawing method so there is only a little  damage spermatozoa during freezing process.  The research has been done  to explore  the  influence of  equilibration time and thawing methode  on  frozen semen quality of garut rams. The results shown that the mean of percentages of progressive motile sperm, percentages of viable sperm, percentages of plasma membrane and acrosomal intact on four hours equilibration (52.50% ;  62.33%; 57.17% and 56.42%)  were not significantly different  (P>0.05) from five hours equilibration (52.27%;  63.67%;  56.92% and 57.58 %)  and six hours equilibration (54.17%; 61.00%; 59.42% and 58.58%) respectively.  The percentages of progressive motile sperm, percentages of viable sperm,   percentages of plasma membrane and  acrosomal intact on the thawing method  on the temperature of 370C  for 30 seconds (53.33% ;  62.39%; 57.94% dan 58.61) were not significantly different  (P>0.05) from the thawing methode  on the temperature of 250C for 45 seconds (52.22% ; 62.28%; 57.72% dan 56.44) respectively.  The conclusion shown that  the treatment of four hours equilibration, five hours equilibration,   six hours equilibration and also  the treatment of thawing method  on the temperature of 370C   for 30 seconds and the thawing methode  on the temperature of 250C   for 45 seconds do not have effect on garut ram freezing semen quality. (Animal Production 7(2): 74-80 (2005) Key  Words : Equilibration, Thawing, Semen, Garut ram
Occurrence of Sperm Abnormality of Beef Cattle at Several Artificial Insemination Centers in Indonesia Arifiantini, RI; Purwantara, B; Riyadhi, M
ANIMAL PRODUCTION Vol 12, No 1 (2010): January
Publisher : Universitas Jenderal Soedirman, Faculty of Animal Science, Purwokerto-Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (411.696 KB)

Abstract

In the most species studied sperm abnormalities have long been associated with male infertility and sterility. This study evaluated the sperm morphology (normality and abnormality) of beef cattle at several Artificial Insemination centers in Indonesia. Total of 142 bulls were used in this study; an ejaculate from each bull was examined. A drop of semen was placed on 3-4 glass slides, and smears were prepared and air-dried. The smears were stained with carbolfluchsin-eosin (Williams stain). Types of morphological abnormalities were recorded from 500 cells on each sample. It was recorded that 77.46% samples had low primary sperm abnormalities (<5%), while the high level of primary sperm abnormalities (>10%) was found in 5.63% samples.  Pear shaped was the most frequently type of abnormality found on examined samples (2.24 ± 2.94%); while double head was the lowest (0.01 ± 0.04%). (Animal Production 12(1): 44-49 (2010) Key words : sperm abnormality, beef catlle, artificial insemination center
SELEKSI DAN KAPASITASI SPERMATOZOA DENGAN METODE PERCOLL GRADIENT VKYUK FERTILISASI OOSIT DAN PRODUKSIEMBRIO IN VITRO PADA SAPI Triwulanningsih, Endang; Toelihere, MR; Yusuf, TL; Purwantara, B; Diwyanto, K; Rutledge, JJ
BERITA BIOLOGI Vol 6, No 3 (2002)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (466.535 KB) | DOI: 10.14203/beritabiologi.v6i3.1214

Abstract

This research has been conducted at the laboratory of in vitro fertilization of the University ofWisconsin, USA. These embryos may be used for improving genetic value of Indonesian cattle. Ovaries were collected from slaughterhouse in Wisconsin. Oocytes were matured in TCM-199 medium enriched with FSH 10 ilm, estradiol 17 P lul/ml and 10 % FCS for 20 hours. The oocytes were fertilized in vitro with motile sperm selected and capacitated by using the percoll gradient with 2 ml vs 0.5 ml per layer as treatment A and B respectively. Sperm and oocytes were incubated in fertilization medium (mTALP) for 20 hours. All zygotes were cultured in CRlaa medium up to btastocyst stage and were fed with serum 5 iV 50 )j.l in culture medium on day 6. Percentages of cleavage, morula, blastocyst, expanded blastocyst, unfertilized and degenerated ova in this study were 86.3 vs 91.6 %, 53.3 % vs 75.9 %; 32.6 % vs 63.4 %; 21.1 % vs 33.0 %; 13.7 % vs 8.4 %, 32.9% vs 15.6 % for treatment A (n=1007) vs B (n=1055), respectively. Based on result of this study, it is concluded that the best method for IVP (in vitro production) of cattle embryos is using percoll gradient with 500 ul per layer.
Co-Authors Amrozi Astuti . D Muchtadi D. Sajuthi Endang Triwulaninngsih Endang Triwulanningsih Herdis . herdis herdis I Arifiantini I Inounu I Supriatna I-K Sutama I.G Putu I.K Sutama J.J Rutledge K Diwyanto Kusuma . M Agil M Astawan M Riyadhi, M M Riza M Surachman M.R Toelihere MR Toelihere, MR MUHAMMAD RIZAL N.M Artiningsihi P Situmorang R Handarini R.K Achjadi RI Arifiantini, RI Riasari Gail Sianturi RTS Adikara Rutledge, JJ T Laswardi Yusuf, T Laswardi T Wresdiyati T.L Yusuf T.L. Yusuf TL Yusuf, TL Wilmientje Marlene Mesang Nalley Yudi .