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All Journal Protobiont: Jurnal Elektronik Biologi Melintas An International Journal of Philosophy and Religion Extension Course Filsafat ( ECF ) ANNALES BOGORIENSES Journal of Agriculture (JoA) J-ENSITEC (Journal of Engineering and Sustainable Technology) JCRIM (Journal of Current Research In Multidisciplinary) Annales Bogorienses
Agus Rachmat
Department of Philosophy, Parahyangan Catholic University, Bandung
Religion Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Engineering Immunology & microbiology Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Mechanical Engineering Medicine & Pharmacology Social Sciences

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In-Vitro Growth of Transgenic Sengon Resulting From Co-Cultivation Using Agrobacterium Tumefaciens Bacteria Aslan, Aura Aulia; Larekeng, Siti Halimah; Rachmat, Agus; Restu, Muhammad
Journal of Agriculture Vol. 3 No. 02 (2024): Research Articles, July 2024
Publisher : ITScience (Information Technology and Science)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47709/joa.v3i02.4465

Abstract

Sengon (Paraserianthes falcataria (L) Nielsen) is a forestry plant often developed for industrial purposes in Indonesia. Sengon is a fast-growing species and has a relatively short-cutting period. Sengon wood is widely used in the production of carpentry wood, plywood raw materials, and pulp. The purpose of this study was to optimize the transformation of the entC and pmsB genes into sengon plants using the embryo injury method through Agrobacterium tumefaciens as an intermediary and to determine the efficiency of the transformation using the injury to sengon embryos. This research was conducted from September 2022 to March 2023. The research was conducted at the Genetically Modified Organism (GMO) Tissue Culture Laboratory, Recombinant Protein (Microbe) Laboratory, and Greenhouse of the Genetic Engineering Center, National Research and Innovation Agency (BRIN) Cibinong, Bogor, Java West. The research procedure consisted of several steps, namely Preparation of MS Media, Preparation of LB Media, Preparation of Sengon Seed Infection Using Agrobacterium tumefaciens, Sterilization and Planting of Sengon Seeds, Acclimatization, and Molecular Analysis (including Sengon DNA isolation, PCR Amplification, and Electrophoresis). Transformation efficiency was calculated by the positive strain of hpt PCR bands compared to the number of transformed embryos and multiplied by 100%. In the results of the PCR analysis, it was found that one plant sample carried the hpt marker, which indicates the integration of the entC and pmsB genes. which when analyzed using transformation efficiency calculations, the data obtained for the entire transformation stage was 0.8%, where the success of the transformation obtained was still classified as low. Factors that cause the low success of transformation include the method of transformation, the growth phase of bacteria, genetic material, and culture aseptic conditions.
Analysis of the Influence Factors of ULP Service Quality on PPK Satisfaction in the Electronic Selection Process of Government Goods or Services Providers in Bandung City Iqlima, Ulfa; Rachmat, Agus; Kadir, Yushar
Journal of Current Research In Multidisciplinary Vol. 2 No. 4 (2024): JCRIM (Journal of Current Research In Multidisciplinary)
Publisher : Yayasan Gema Bina Nusantara

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Abstract

Electronic Procurement Services (LPSE) aim to support transparent, efficient, and accountable government procurement of goods and services. This study assesses the satisfaction of Commitment Making Officers (PPK) with the services of the Bandung City Procurement Service Unit (ULP) using a descriptive analytical method. We collected data using a questionnaire that measured variables such as reliability, responsiveness, assurance, empathy, and tangibles. The results of the study showed that the PPK satisfaction index was in the satisfied category, with several aspects, such as speed of service and competence of ULP workers, being the dominant indicators. However, the study identified several weaknesses, such as inadequate two-way communication and insufficient physical facilities. This study provides recommendations for improving ULP services by strengthening communication, providing more adequate facilities, and offering ongoing workforce training. This study aims to improve the quality of electronic procurement of goods and services in Bandung City, thereby fostering better governance.
Tillich’s View on the Development of Capitalism (Part II) Rachmat, Agus
MELINTAS An International Journal of Philosophy and Religion (MIJPR) Vol. 26 No. 2 (2010)
Publisher : Faculty of Philosophy, Parahyangan Catholic University, Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26593/mel.v26i2.879.125-140

Abstract

Historically, the original impulse of capitalism is to be the emancipatory social power to liberate mankind from the fetter of Religious Leviathan reigning in the dogmatic Medieval Era. Ironically, after its historical global triumph, it becomes an Economic Leviathan of its own. The acute sense of structural sin and existential finitude nurtured by religious consciousness should make us aware of the hidden carnivorous dimension working deeply in the capitalist system that creates waste, crisis and destruction due to the cultivation of instant profit and unbridled greed as the moving forces of capitalism. KeyWords:*Watchman State, *Technical Reason, *Planning Reason, *Economic Leviathan, *Fascism, *Communism, *Global Capitalism, *SocialMarket, *Welfare State .
TILLICH’S VIEW ON THE DEVELOPMENT OF CAPITALISM Rachmat, Agus
MELINTAS An International Journal of Philosophy and Religion (MIJPR) Vol. 25 No. 3 (2009)
Publisher : Faculty of Philosophy, Parahyangan Catholic University, Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26593/mel.v25i3.917.353-365

Abstract

Driven by his method of correlation, Paul Tillich critically analyzes the social condition of modern society which is characterized by the growing hegemony of capitalism. Capitalism is viewed not simply as a certain economic arrangement of society but as the embodiment of the religion of reason, namely, the historical project of humanity to realize its utopian goals and longings by the power of science and technology alone. However, by forgetting the awareness of the inherent finitude of reason cultivated by a genuine religious consciousness, mankind is threatened to lose sight of the demonic dimension built-in structurally in the capitalist mode of wealth creation.
Landasan Etis Kegiatan Ekonomi Rachmat, Agus
MELINTAS An International Journal of Philosophy and Religion (MIJPR) Vol. 21 No. 1 (2005)
Publisher : Faculty of Philosophy, Parahyangan Catholic University, Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26593/mel.v21i1.2640.47-74

Abstract

This article investigates the deeper foundation for the rapidly emerging and widespread consciousness in the business world on tbe nature and demand of the corporate social responsibility. Ethical norms and values should no longer be considered as externalistics, annoying additional costs and burdens to business interprices, but the very inner motivation for doing business at all, namely, to bring betterment to the world-wide human condition. That enduring noble al could only be accomplished if there is a comprehensive understanding on the kind of rationality that should permeate the so-called market society. In addition to the instrumental rationality that presently makes the market fluorish, we should also integrate what Max Weber called affective rationality and normative rationality.
Genetic Transformation of Rice cv. Ciherang Using Double T-DNA Vector Harboring cry1Ab Gene Sulistyowati, Yuli; Rachmat, Agus; Zahra, Fatimah; Rachmawati, Syamsidah; Nugroho, Satya
Annales Bogorienses Vol. 15 No. 1 (2011): Annales Bogorienses
Publisher : BRIN

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Abstract

Rice stem borer (Scirpophaga sp.) is considered as one of the primary pests causing yield decrease in Indonesia. Genetic engineering is considered as one of the most effective way to improve the resistance of rice plants to yellow stem borer since no resistance gene has been found in rice and its wild relatives. A double T-DNA binary vector p2TDNAcryIAb wich carry two independent T-DNA was used to transform Indonesian elite rice cv Ciherang using Agrobacterium-mediated transformation to generate transgenic rice that free from selectable marker genes. One T-DNA containing a selectable marker hygromycin phosphotransferase (hpt) gene and the other carrying the cry1Ab gene. As many as 37 putative transgenic plants from 14 embryos were generated. PCR analysis demonstrated that 22 out of 37 putative transgenic rice contain cry1Ab gene. All Ciherang rice carrying the cry1Ab gene expressing Cry1Ab protein as shown by the immunostrip assay. Further work will be carried out to determine the transgenes copy number, to select marker free transgenic rice, and to evaluate the resistance of transgenic rice against neonates larvae of yellow stem borer.
Isolation and Characterization of OsNAC6 cDNA from Rice (Oryza sativa L.) cv. Nipponbare, Batutegi, and Rojolele Rachmat, Agus; Nugroho, Satya; Nurdiani, Dini; Swastika, Maria; Sukma, Dewi; Aswidinnoor, Hajrial; Sudarsono, Sudarsono
Annales Bogorienses Vol. 18 No. 2 (2014): Annales Bogorienses
Publisher : BRIN

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Abstract

Transcription factors have an important function in regulating gene expression and plant responses to stresses. The ERF, bZIP, WRKY, MYB, and NAC are stress inducible transcription factors. The OsNAC6 is a member of the NAC transcription factor family in rice and its expression is induced by abiotic stresses, wounding and blast disease. Characterization of OsNAC6 gene sequences would give a better understanding on how OsNAC gene functions biologically. The objectives of this research are to isolate the OsNAC6 cDNA from Nipponbare, Batutegi, and Rojolele cultivars, to characterize their DNA sequences, and to compare their sequences to other NAC genes from other plants available in GenBank DNA databases. Isolated cDNA and sequencing of the fragments resulted in a 912 bp DNA sequences. Translation of the sequences yielded a protein consisted of 303 amino acid residue. Blast analysis of amino acid sequences indicated identity of isolated cDNA from three Indonesian rice cultivars are the OsNAC6 gene. Deduced amino acid residues from amplified cDNAs of Nipponbare, Batutegi, and Rojolele cultivars shared 100% sequence identities to rice OsNAC6 (Acc. # BAA89800), 71-100% sequence identity to a number of OsNAC protein from Oryza sativa and 63-83% sequence identity to NAC protein from other plants.
Analysis of Delay Factors in Drinking Water Supply System Development Projects on Small Islands (Case study of the Drinking Water Supply System Project on Maratua Island) Febiana, Indra; Rachmat, Agus; Kadir, Yushar
Journal of Current Research In Multidisciplinary Vol. 3 No. 2 (2025): JCRIM (Journal of Current Research In Multidisciplinary)
Publisher : Yayasan Gema Bina Nusantara

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Abstract

To fulfill cost, quality, and schedule restrictions, construction projects must carefully plan, execute, and oversee project management. Construction delays frequently raise expenses and lower quality. An operational Water Supply System (SPAM) is essential in distant places like tiny islands. The Seawater Reverse Osmosis (RO) SPAM Project on Maratua Island deviated 6% from the contract timeline. This study analyzes and ranks delay factors in SPAM project development on Small Islands. This study collected primary data from 90 respondents (30 Consultants, 30 Executors/Contractors, and 30 Owners/Ministry of PUPERA) using non-experimental surveys. Validity and reliability testing, the Index of Importance for ranking, and Factor Analysis (PCA) to minimize and cluster the 23 original variables were performed. PCA divided the variables into three main categories that explained 61.808% of variation. Transportation, Geographical Location, Material, and Equipment were the biggest factor, accounting for 51.801% of variation. This component includes logistical challenges like distant site transportation and import delays. Second was Manpower, Scope Changes & Work Documents (5.404%), then Planning and Coordination (4.603%). Poor contractor job performance was the biggest delay issue. To reduce delays in small island SPAM projects, logistics planning and integrated multi-party project management must be urgently evaluated.
Co-Authors Aslan, Aura Aulia Dewi Sukma DINI NURDIANI Fatimah Zahra Febiana, Indra HAJRIAL ASWIDINNOOR Hery Widijanto HERYADIANA, ASEP DIAN Iliana, Iliana Iqlima, Ulfa Kadir, Yushar LATIFFAH ZAKARIA Mukarlina, Mukarlina Rachmawati, Syamsidah Rahmawati, Rahmawati Restu, Muhammad Satya Nugroho SITI HALIMAH LAREKENG, SITI HALIMAH Sudarsono Swastika, Maria Swastika, Maria Syamsidah Rahmawati, Syamsidah Yuli Sulistyowati