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SEPARASI SPERMATOZOA X DAN Y MENGGUNAKAN LEVEL ALBUMIN YANG BERBEDA SEBAGAI MEDIA PEMISAH SPERMATOZOA BABI I Made A. Sudarma; W Marlene Nalley; Henderiana L.L. Belli; Aloysius Marawali
JURNAL NUKLEUS PETERNAKAN Vol 1 No 1 (2014): Juni 2014
Publisher : Universitas Nusa Cendana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/nukleus.v1i1.705

Tujuan dari penelitian ini adalah untuk mempelajari pengaruh penggunaan albumin dengan tingkatyang berbeda untuk memisahkan spermatozoa X dan Y sebagai media untuk memisahkan spermababi jantan. Sperma dipisahkan menggunakan tingkat yang berbeda dari albumin dengan 4perlakuan: rasio antara fraksi atas dan fraksi bawah: 10 dan 30%, 10 dan 40%, 10 dan 50%, 10 dan60% selama 1 jam. Hasil penelitian menunjukkan bahwa proporsi terbaik sperma X oleh fraksi disemua pelakuan, dan juga proporsi terbaik dari sperma Y oleh fraksi bawah pada semua lokasi.Proporsi X:Y dari semen segar (51,68: 48,32 ± 2,66) berbeda (P <0,05) dibandingkan dengan setiapperlakuan sampai fraksi atas (T1 – 74,02: 25,98 ± 5 , 65); T2 – 64,14: 35,86 ± 3,41); T3 – 62,29:37,71 ± 3,98), dan T4 – 59,26: 40,74 ± 4,33)) dan juga fraksi bawah (T1 – 38,13: 61,87 ± 7,69); T2– 35,48: 64,52 ± 6,11); T3 – 30,37: 69,63 ± 4,93); dan T4 – 26,41: 73,59 ± 4,54)). Viabilitas dankelainan sperma setelah dipisahkan tidak berbeda dalam setiap perlakuan. Konsentrasi spermasetelah perpisahan di fraksi atas lebih dari fraksi bawah di semua perlakuan. Sperma babi tidakterpisah selama serangkaian perlakuan dari proses pencucian hingga proses pemisahan dalammedia albumin yang mengakibatkan penurunan motilitas sperma dari 73 ± 3% menjadi 6,5 ± 0,58%pada fraksi atas pada perlakuan pertama sampai 3 ± 0,82% di fraksi bawah pada perlakuan keempatdi akhir pemisahan. Disimpulkan bahwa perlakuan terbaik untuk mendapatkan sperma X olehkonsentrat 10 dan 30% sedangkan sperma Y yang oleh konsentrat 10 dan 60%, sperma setelahperpisahan baik fraksi atas maupun fraksi bawah memiliki motilitas yang sangat rendah.

TAMPILAN REPRODUKSI INDUK BABI LANDRACE HASIL INSEMINASI BUATAN PADA PARITAS YANG BERBEDA Petronela Lotu; Henderiana Laura Louisa Belli; Aloysius Marawali
JURNAL NUKLEUS PETERNAKAN Vol 4 No 2 (2017): Desember 2017
Publisher : Universitas Nusa Cendana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/nukleus.v4i2.827

The purpose of the present study was to determine the reproductive performance of landrace crossbreed sows which being maintained at the village level. Eighteen crossbreed landrace sows and one landrace-male pigs as a source of semen were used in this study following a randomized complete block design with three treatments and six replications. The treatments were: P1 (first and second parity); P2 (third and fourth parity); and P3 (fifth and sixth parity). The parameters observed were: length of estrus, service per conception rate (S/C), conception rate (CR) and litter size. Data were analyzed using Anova. There were no significant difference (P>0.05) between treatments on length of estrus, service per conception rate (S/C), conception rate (CR) and litter size. However, the results indicate that length of estrus of landrace crossbreed sows were: 3.16 days; 3.33 days and 3.16 days for P1, P2 and P3, respectively. The services per conception obtained were: 1.16 times; 1.33 times and 1.16 times for P1, P2, and P3, respectively. Furthermore, conception rates were: P1 = 83.33%; P2 =100% and P3 = 100%. Litter size obtained were: 8.4; 8 and 8.6 for P1, P2 and P3, respectively. ABSTRAK Suatu penelitian terhadap kinerja reproduksi babi betina yang berbeda paritas telah dilaksanakan di Desa Penfui Timur, Kecamatan Kupang Timur, Kabupaten Kupang selama (4) bulan. Tujuan dari penelitian ini adalah untuk mengetahui kinerja dari babi–babi induk betina peranakan landrace yang sedang dipelihara ditingkat peternakan rakyat. Penelitian ini menggunakan 18 ekor babi betina bangsa peranakan landrace dan satu ekor babi jantan bangsa landrace sebagai sumber semen untuk keperluan inseminasi. Penelitian menggunakan rancangan acak kelompok dengan tiga perlakuan dan 6 ulangan.Perlakuan tersebut adalah: P1 (paritas pertama dan kedua); P2 (paritas ketiga dan keempat); dan P3 (paritas kelima dan keenam). Parameter yang diamati yaitu: lama estrus, service per conception rate (S/C), conception rate (CR) dan litter Size. Data dianalisis dengan menggunakan anova. Hasil penelitian menunjukkkan bahwa lama berahi babi betina peranakan landrace yaitu P1= 3,16 hari; P2= 3,33 hari dan P3= 3,16 hari. S/C yang diperoleh adalah: P1= 1,16 kali; P2= 1,33 kali dan P3= 1,16 kali. Selanjutnya angka kebuntingan P1 = 83,33 %; P2= 100% danP3= 100%. Litter size P1= 8,4 ekor; P2= 8 ekor dan P3= 8,6 ekor. Hasil analisis statistik menunjukan bahwa perlakuan berpengaruh tidak nyata terhadap lama berahi, angka kebuntingan (CR), service per conception (S/C) dan litter size (P>0.05).

PENGARUH PEMBERIAN EKSTRAK HIPOFISA DAN PROBIOTIK ABG-O PADA ANAK BABI PERSILANGAN TERHADAP PENINGKATAN BERAT BADAN SAPIH, UKURAN LINEAR TUBUH DAN METABOLIT DARAH (The effect of giving pituitary and probiotic extracts abg-o to piglets crosses.....) Mardemus Misa; Wilmientje M Nalley; Thomas Mata Hine; Twenfosel Dami Dato; Maritje A Hilakore; Mariana Nenobais; Aloysius Marawali
JURNAL NUKLEUS PETERNAKAN Vol 7 No 1 (2020): Juni
Publisher : Universitas Nusa Cendana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/nukleus.v7i1.2256

The purpose of this study was to determine the effect of giving pituitary extract (EH) and probiotics ABG-O on piglets crossing to increase weaning weight, linear size of body and blood metabolites. The research material was a crossbred of landrace and duroc aged 2 weeks, as many as 24 birds. The experimental design was a completely randomized design with three treatments and eight replications. The three treatments are P0 (control), P1 (EH injection 1 mL), and P2 (injection EH 1 mL + probiotic ABG-O 2 mL). EH injections are carried out every 5 days and administration of probiotics is given orally using spoit every day until the piglets are weaned. The parameters observed were weight gain, linear size of body and blood metabolites. This data was analyzed by ANOVA and continued with Duncan test. The results of this study indicate that the average daily weight gain of crossbred piglets in treatments P0, P1, and P2 are 0.11; 0.13; 016 kg, body length (0.40; 0.45; 0.44 cm), chest circumference (0.29; 0.27; 0,30 cm), and shoulder height (0.25; 0.24; 0.25 cm). Blood glucose levels (117.31; 112.85; 117.25) and total plasma protein (4.60; 4.90; 4.80). The results of the statistical analysis showed that the EH and ABG probiotic treatments affected the UN (P<0.05) but did not affect (P> 0.05) the size of the linear body and blood metabolites. It can be concluded that the administration of pituitary extracts and ABG probiotics can increase the weaning weight but does not affect the linear size of the body blood metabolites and total protein plasma of crossing piglets.

PENGARUH PENAMBAHAN EKSTRAK BAWANG MERAH (Allium cepa) DALAM PENGENCER TRIS - KUNING TELUR TERHADAP KUALITAS SPERMATOZOA SAPI BALI PADA PENYIMPANAN IN VITRO (The effect of additional onion (Allium cepa) extract in tris - egg yolk extender on the sperm... Fian K. Djita; Wilmientje M. Nalley; Thomas M. Hine; Aloysius Marawali
JURNAL NUKLEUS PETERNAKAN Vol 8 No 2 (2021): Desember
Publisher : Universitas Nusa Cendana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/nukleus.v8i2.4304

The purpose of this study was to determine the effect of adding onion extract (OE) to Tris-egg yolk (T-EY) diluent on the quality of bali bulls spermatozoa. The semen of three bali bulls aged 3-4 years were collected twice a week using the artificial vaginal method. Semen with good quality, motility 79%, viability 84.63%, abnormality 2.93% diluted with T-EY diluent with the addition of OE: T0: 0%; T1 1%; T2: 2%; T3: 3%; T4: 4%; and T5: 5%. The diluted semen was preserved in a refrigerator at 3-5 °C and evaluated for the quality of the spermatozoa every 24 hours. The results showed that the addition of OE in T-EY diluent had a positive effect on the quality of bali bulls spermatozoa. The addition 3% of OE (T3) in T-EY diluent resulted in higher spermatozoa quality (P <0.05) than the other treatments, namely motility 42.01%, viability 48.74%, and abnormalities 4.13% on the sixth day of preservation. It was concluded that the addition of onion extract in Tris - egg yolk diluents could improve the quality of bali bulls spermatozoa, with the best level of onion extract being 3%.

PENGARUH PERBEDAAN WAKTU EKUILIBRASI TERHADAP KUALITAS SEMEN BEKU BABI LANDRACE DALAM PENGENCER DURASPERM MODIFIKASI DENGAN AIR BUAH LONTAR (Effect of different equilibration time on the quality of landrace boar frozen semen on modification diluent.... Maria G. Mega; Wilmientje M. Nalley; Aloysius Marawali; Henederiana L. L. Belli
JURNAL NUKLEUS PETERNAKAN Vol 9 No 1 (2022): Juni
Publisher : Universitas Nusa Cendana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/nukleus.v9i1.4988

An experiment was carried out to recognize the effect of long equilibration time on the quality of spermatozoa and to obtain optimal equilibration time in the process cryopreservation of landrace pig semen. Semen was obtained from a landrace boar aged 2-3 years using glove hand method. The semen was than evaluated macroscopically and microscopically, good quality semen was diluted in a basic diluent, than kept for two hours at a temperature of 27-28oC. The semen were then centrifuged at 2000 rpm for 15 minutes, the supernatant was discarced and the pellets was re-diluted into basic diluent with a ratio of 1:1. Then, semen was diluted in treatment diluent with modified durasperm + 6% lactose + 4% glycerol with a concentration of 500 x 106/0.5 mL. Furthermore, the semen was packaged in 0.5 ml straws, then equilibrated at a temperature of 3-5oC appropriate to treatment, with 1 hour equilibration (T1), 2 hours equilibration (T2) and 3 hours equilibration (T3). After equilibration, the straws was frozen horizontally 5 cm above the liquid N2 vapor for 10 minutes at a temperature of ± -110oC and then stored in a liquid N2 container (-196oC). To test frozen semen, it was done by stored frozen semen at 37oC for 30 seconds. The results showed that the highest quality of spermatozoa (P <0.05) was found in T2 and T3 when compared to T1 (P> 0.05), with the motility value was T2: 28.13 ± 2.39%; T3: 25.00 ± 4.08%; and T1 18,15±3,23%. It can be concluded that 2 hours and 3 hours equilibration time was able to maintain the quality of frozen semen landrace boars and the statistically was not significant.

KUALITAS SEMEN BEKU BABI DUROC DALAM PENGENCER TRIS MODIFIKASI DENGAN WAKTU EKUILIBRASI YANG BERBEDA (The frozen sperm quality of duroc pig in modified tris diluent with different equilibration time) Selsiana Dongkot; Aloysius Marawali; Thomas M. Hine; Wilmientje M. Nalley
JURNAL NUKLEUS PETERNAKAN Vol 9 No 1 (2022): Juni
Publisher : Universitas Nusa Cendana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/nukleus.v9i1.5491

This study aimed to examine the quality of frozen sperm of duroc boar in modified Tris diluent with different equilibration time. Semen was collected from two duroc males and evaluated macroscopically and microscopically. Semen with motility 75%, concentration 200x106 cell/mL and abnormality <20% was diluted in Tris modified diluent, then held for two hours at room temperature (27-28ºC). The semen that has been in the holding time is then centrifuged at 2000 rpm for 15 minutes. The pellet from centrifuge were diluted again with modified Tris diluent + 3% glycerol. The diluted semen was packed in 0,50 mL mini straws and equilibrated according to the treatment equilibration time, namely P1: 1 hour equilibration time, P2: 2 hour equilibration time, P3: 3 hour equilibration time. After equilibration , it was frozen over liquid nitrogen vapor (-110ºC) at a distance of 5 cm for 10 minutes, then stored in liquid nitrogen (-196ºC) and left for 24 hours. Then thawing with warm water at 37ºC for 30 seconds in a water bath. The result showed that motility, viability, abnormality, intact plasma membrane and recovery rate were significantly higher (P<0,05) at two hour equilibration , namely 23.12%, 45.44%, 4.65%, 50,15%, 33.03% respectively compared to one and two hour equilibration time, namely 20%, 42.17%, 8.19%, 42.67%, 28.57% and 16,87%, 36.39%, 7.64%, 37.73%, 24.99% respectively. This study conclude that the equilibration time of two hours resulted in higher quality of frozen sperm of duroc boar than one and three hours.

PENGARUH PENAMBAHAN BEBERAPA LEVEL GLUTATHIONE DALAM PENGENCER AIR KELAPA KUNING TELUR TERHADAP KUALITAS SEMEN SAPI ANGUS (The effects of adding some levels of glutathione in egg yolk coconut water on the quality of angus bull semen) Desiderius S. Agung; Aloysius Marawali; Kirenius Uly; Franky M.S. Telupere
JURNAL NUKLEUS PETERNAKAN Vol 10 No 1 (2023): Juni
Publisher : Universitas Nusa Cendana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/nukleus.v10i1.7948

The purpose of this study was to determine the effect of adding several levels of glutathione in egg yolk coconut water dilution (EY-CW) on the quality of angus bull semen during storage. The material used is fresh semen from one 3 years old angus bull that has good quality. Good quality semen was dilut with EY-CW diluent with the addition of glutathione at the levels of 0% (T0), 0.1% (T1), 0.2% (T2), and 0.3% (T3). After dilution, the semen stored at 3-5oC. Evaluation of semen after dilution was carried out every 24 hours of storage for motility, viability, abnormalities and survival of spermatozoa. The research data were analyzed with analysis of variance and continued with the Duncan test. The results showed that the addition of 0.2% glutathione level (T2) had the best quality (P<0.05) compared to other treatments, namely motility (47.00±2.09%), viability (57.64±3.08%), abnormality (6.20±1.29%) and survival (4.00±0.00 days). It was concluded that the addition of a glutathione level of 0.2% in the AK-KT diluent provided the best and more effective influence in maintaining the quality of angus bull liquid semen until the fourth day of storage.

Spermatozoa quality of Landrace Boar in Beltsville thawing solution diluent with various levels of moringa leaf extract Wirona Adu; Thomas Hine; Aloysius Marawali; Wilmientje Marlene Nalley
Jurnal Ilmu Peternakan Terapan Vol 7 No 1 (2023): Jurnal Ilmu Peternakan Terapan
Publisher : Politeknik Negeri Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25047/jipt.v7i1.4027

This study aims to find the best level of sun-dried Moringa leaf extract (SDMLE) in Beltsville Thawing Solution (BTS) diluent to preserve the spermatozoa of Landrace boars. Semen from a two-year-old Landrace boar was collected using the massage method. Semen with spermatozoa motility ≥70% and spermatozoa abnormalities <20% was diluted with BTS (P0, control), BTS + 3% SDMLE (P1), BTS + 6% SDMLE (P2), BTS + 9% SDMLE (P3), and BTS + 12% SDMLE (P4). Storage of liquid semen using a cool box with a temperature of 15-20oC, while evaluating the quality of semen every 8 hours for spermatozoa motility, viability, abnormality, and longevity. The results showed that spermatozoa stored for 40 hours in BTS + 3% SDMLE (P1) diluent had higher quality (P<0.05) than the other four diluents, namely motility (54.00%), viability (61.16%), abnormality (3.62%), and longevity (45.77%). The results of this study concluded that 3% SDMLE added to BTS diluent was the best level for the preservation of spermatozoa in landrace boars.

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