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All Journal Journal of Parasite Science Journal of Applied Veterinary Science and Technology

Rahmi Sugihartuti

Department Of Basic Veterinary Medicine - Faculty Of Veterinary Medicine, Universitas Airlangga

Author-ID : 3558473

Agriculture, Biological Sciences & Forestry Immunology & microbiology Veterinary

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Anthelmintic Potential Extract Mango Gadung Seed (Mangifera indica L.) Mecistocirrus digitatus in vitro Ria Nikmatul Jannah; Sri Mumpuni Sosiawati; Sri Chusniati; Kusnoto Kusnoto; Rahmi Sugihartuti; Setiawati Sigit
Journal of Parasite Science (JoPS) Vol. 1 No. 2 (2017): Journal of Parasite Science
Publisher : Universitas Airlangga

This research aim to attest the potential anthelmintic extract mango seed gadung (Mangifera indica L.) against worms Mecistosirrus digitatus in vitro, the study also aims to determine the effect of immersion time, variasis concentration and the relationship between time and treatment. this study used 210 M. digitatus worm extracted regardless of gender worms. treatment that is given was the concentration extract mango seed gadung was 5%, 7.07%, 10%, 14.14%, 20%, negative control using NaCl physiological and comparison using levamisole Hidrokloride 0.0025 mL. Observations death worm Mecistocirrus digitatus and observations were made at the 6th hour, 12th hour, 18th hour and 24th hour. The results showed that variations in concentration, soaking time and the relationship between soaking time with the treatment affect mortality Mecistocirrus digitatus worms. Test results of the analysis statitistik using factorial Anova and Duncan's Multiple Range Test showed a significant difference (p<0.01) between the time factor, variations in concentration and relations between time and treatment.

The Anthelmintic Activity of Ethanol Extract of Bitter Leaf (Vernonia amygdalina) Against Ascaridia galli Worm in Vitro Amelia Dwita Safitri; Iwan Sahrial Hamid; Poedji Hastutiek; Setiawan Koesdarto; Rahmi Sugihartuti; Endang Suprihati
Journal of Parasite Science (JoPS) Vol. 3 No. 1 (2019): Journal of Parasite Science
Publisher : Universitas Airlangga

The aims of this study is to know the anthelmintic activity of ethanol extract of bitter leaf (Vernonia amygdalina) against Ascaridia galli worm in Vitro, as well as knowing effective concentration 50 (EC50) and lethal time 50 (LT50). Method that used in the research was completely randomized design. There were five treatments of physiological NaCl solution (K-), piperazine sitrate (K+), etanol extract of bitter leaf 0,35% (P1), etanol extract of bitter leaf 1,4% (P2), etanol extract of bitter leaf 4,2% (P3), and each treatment was done in four replications. This research used ten Ascaridia galli in each treatment for all replications. The observation and recording of dead Ascaridia galli was done at 2, 4, 6, 8, 10 and 12 hours. Ascaridia galli were declared dead if there was no movement when disturbed by anatomy tweezer and when dipped in slightly warm water (50°C). The obtained data was analyzed using ANAVA and continued with Duncan Multiple Range Test. The result of this research show that etanol extract of bitter leaf (Vernonia amygdalina) has anthelmintic effects against Ascaridia galli worm in Vitro. In the extract with 4,2% concentration, there is anthelmintic property that almost the same as Piperazine sitrate 10 mg/ml. the higher the concentration of extract, the higher the property of anthelmintic. In probit analysis show that EC50 achieved by concentration 2.093% with the low concentration of .002% and the highest concentration of 3.632%. LT50 of ethanol extract of bitter leaf was 0.35% at 10.323 hours, 1.4% at 9.800 hours, 4.2% at 7.864 hours and Piperazine sitrate 10 mg/ml at 9.013 hours.

The Potential of Anting – Anting (Acalypha indica L.) Leaf Extract as Anti-Scabies to Sarcoptes scabiei var. Cuniculi in vitro Luluk Tri Astuti; Rahmi Sugihartuti; Lianny Nagoi; Nunuk Dyah Retno Lastuti; Dewa Ketut Meles; Agus Sunarso
Journal of Parasite Science (JoPS) Vol. 3 No. 2 (2019): Journal of Parasite Science
Publisher : Universitas Airlangga

The Aim of research to determine the potential of anting-anting (Acalypha indica L.) leaf extract as anti-scabies to Sarcoptes scabiei var. Cuniculi in vitro by observing the mortality rates and lethal concentration of Sarcoptes scabiei. There were 5 treatments namely DMSO 0.5% (PN). Amitraz (P0), anting-anting (Acalypha indica L.) leaf extract concentratrion of 6,25% (P1), 12,5% (P2) and 25% (P3). Observations were carried out for 8 hours with stereomicroscope. The data was analyzed using rpobit analysis with SPSS 20.0 software. The results showed that the fastest mortality rate of S. Scabiei was group p3 at 4.6 mites/hour., then P0, P2, P1 and PN respectively 3.5, 3.1, 1.9, and 1.3 mites/ hour. The smallest concentration of anting-anting (Acalypha indica L.) leaf extract was not significantly different compared to amitraz for killing of 50% and 90% S. Scabiei was 12.5% with lethal time (LT50) 1.82 hours and (LT90) 3.69 hours. In conclusion, anting-anting (Acalypha indica L.) leaf extract has mortality rate against S. Scabiei var. Cuniculi of 3.1 mites/hour, lethal concentration of 12.5% and potential as anti-scabies in vitro.

The Potency of Anting Anting (Acalypha indica L.) Leaf Extract as An Acaricide on Boophilus microplus in Larvae and Adult Stages In Vitro Fitri Nurdiana; Rahmi Sugihartuti; Bambang Sektiari Lukiswanto
Journal of Parasite Science (JoPS) Vol. 5 No. 1 (2021): Journal of Parasite Science
Publisher : Universitas Airlangga

The purpose of this study was to determine the potency of anting-anting leaf extract (Acalypha indica L.) as an acaricide against larval and adult Boophilus microplus in vitro by observing the mortality rate of B. microplus. The present study design was a completely randomized design. This study used five treatments, including: Tween 80 1% and aquadest (K-), Neguvon (K+), anting-anting leaf extract with a concentration of 3.125% (P1), 6.25% (P2), and 12.5% (P3 ). Each treatment used 5 repetitions and each repetition used 6 B. microplus larval stages and 5 B. microplus adults. Observations were made for 5 hours for the larval stage of B. microplus and 24 hours for the adult stage of B. microplus. Boophilus microplus is declared dead if there is no movement at all when touched with a needle. The data obtained were analyzed using factorial ANOVA and continued with Duncan's multiple distance test. The results showed that the 12.5% anting anting leaf extract treatment showed no significant difference with the treatment using Neguvon. (p>0.05) in larval stage B. microplus, but in adult B. microplus showed a significant difference with Neguvon (p<0.05). The results of the statistical test can be concluded that the extract of anting-anting leaf (A. indica L.) has the potential as an acaricide of B. microplus larval stage in vitro. The higher the concentration of anting anting leaf extract, the higher the acaricide activity.

Co-Authors Aldin Akbar Rahmatullah Amelia Dwita Safitri Anggreani Desi Ramadhani Rahajeng Bambang Sektiari Lukiswanto Chusniati, Sri Cindy Ercha Aulia Putri Endang Suprihati Fitri Nurdiana Hani Plumeriastuti Iwan Sahrial Hamid Iwan Sahrial Hamid Kusnoto Kusnoto Lianny Nagoi Luluk Tri Astuti Meles, Dewa Ketut Mohammad Sukmanadi Nunuk Dyah Retno Lastuti Poedji Hastutiek Ria Nikmatul Jannah Setiawan Koesdarto Setiawati Sigit Sri Mumpuni Sosiawati Sunarso, Agus

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