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All Journal Jurnal Sain Veteriner Indonesian Journal of Tropical and Infectious Disease Aceh Journal of Animal Science Biosaintifika: Journal of Biology & Biology Education Jurnal Medik Veteriner Journal of Aquaculture and Fish Health Journal of Parasite Science Ovozoa: Journal of Animal Reproduction journal of Basic Medical Veterinary Jurnal Kedokteran Hewan Journal of Fisheries & Marine Jurnal Agro Veteriner

Setiawan Koesdarto

Departemen Parasitologi Veteriner, Fakultas Kedokteran Hewan, Universitas Airlangga

Author-ID : 515120

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Health Professions Immunology & microbiology Medicine & Pharmacology Public Health Veterinary

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Evaluation of Salmonella sp Contamination and its Antibiotics Resistance Patterns Isolated from Broiler Meat Sold at Wet Market in Center of Surabaya Aprillian, Risky; Rahardjo, Dadik; Koesdarto, Setiawan
Indonesian Journal of Tropical and Infectious Disease Vol 5, No 6 (2015)
Publisher : Institute of Topical Disease

Show Abstract | Download Original | Original Source | Check in Google Scholar

Antibiotic resistance now days become a main issue to the medical researches as found many positive result of antibiotic resistance test. One of the causes of antibiotic resistance is using antibiotic as a feed additive to animal. Bacteria that are resistant to antibiotics can be a danger to humans, in this case the resistant bacteria as a result of treatment errors animals, especially chickens that uses low-dose antibiotics as growth promoters. This study aimed to determine the contamination of Salmonella sp and its antibiotics resistance patterns of Salmonella sp isolated from broiler meat sold at wet market in the Center of Surabaya: (a) Pasar Kembang, (b) Pasar Kupang, (c) Pasar Dukuh Kupang, (d) Pasar Kedungsari, (e) Pasar Kedungdoro and (f) Pasar Keputran. The method that used in this study was bacteriological isolation and identification method. The method started with pre-enrichment using Buffered Pepton Water, selective enrichment using Tetrathionate Broth and Selenite Cysteine broth, selective media using Salmonella-Shigella Agar, Biochemical test using Triple Sugar Iron Agar, Simon Citrate, Methyl Red â Voges Proskauer, and Sulfide Indol Motility, and followed with susceptibility test according to Kirby-Bauer method using Mueller-Hinton Agar. The antibiotics that used in susceptibility test were: (a) Meropenem, (b) Ampicillin Sulbactam, (c) Amikacin, (d) Ofloxacin and (e) Nalidixic Acid. The results of this study were found 90% or 27 of 30 samples positive contaminated with Salmonella sp. The results of antibiotics resistance from 27 isolates 0% were resistant to Meropenem, 0% were resistant to Amikacin; 3.7% were resistant to Ampicillin-Sulbactam; 11.1% were resistant to Ofloxacin and 44.4% were resistant to Nalidixic Acid.

Study on the Morphology of Fasciola gigantica and Economic Losses due to Fasciolosis in Berau, East Kalimantan Prasetya, Muhammad Rofi'; Koesdarto, Setiawan; Lastuti, Nunuk Dyah Retno; Suwanti, Lucia Tri; Kusnoto, Kusnoto; Yunus, Muchammad
Biosaintifika: Journal of Biology & Biology Education Vol 11, No 1 (2019): April 2019
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Fasciolosis is one of the endemic parasitic diseases in Indonesia and causes significant economic losses. Fasciola gigantica is the main species found to infect livestock. This research aimed to describe the morphology of F. gigantica histologically using hematoxylin and eosin staining as well as ultrastructure morphology using scanning electron microscopy (SEM). Besides, the study was also carried out to determine the prevalence and estimation of economic losses due to fasciolosis in Berau Regency. The histological identification of flukes morphology found several organs including oral sucker (OS), ventral sucker (VS), cirrus (C), genital pore (GP), uterus (U), ovary (O), tegument (Te), testis (T), and caecum (C). In this study, ultrastructure morphology was divided into anterior and midbody region. In the anterior region, there are an oral sucker (OS) with a diameter of 304.5 µm and a ventral sucker (VS) with a diameter of 571 µm. It was also found the anterior spine with a width of 25.4 µm and a height of 22.8 µm. In the midbody, the spine has a width of ± 26.7 µm and a length of ± 38.9 µm leading posteriorly. Postmortem examination results showed that the prevalence of fasciolosis in Berau regency was 21.65% or 34 of 157 cows which were positive with an estimated loss of 228,656,475.00 IDR/16,450 USD (1 USD=13,900 IDR). The amount of loss due to fasciolosis must be a particular concern in carrying out control and prevention measures. Histology and ultrastructure morphology can become a reference in the development of anthelmintic testing that has been used so far in Indonesia.

KORELASI ANTARA PADAT TEBAR DENGAN INFESTASI EKTOPARASIT PADA UDANG VANAME (Litopenaeus vannamei) DI TAMBAK SUPER INTENSIF Adiacahya, Eren; Koesdarto, Setiawan; Mahasri, Gunanti
Jurnal Sain Veteriner Vol 38, No 2 (2020): Agustus
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.54894

Teknologi budidaya udang yang sudah banyak digunakan di pertambakan di Indonesia, adalah teknologi budidaya yang menggunakan pola super intensif, dengan menggunakan padat tebar yang tinggi, yaitu lebih dari 150 ekor/meter persegi. Padat tebar yang tinggi tersebut dapat mengakibatkan kondisi air sebagai media mengalami penurunan, sehingga menyebabkan udang mengalami stres dan penurunan pertahanan tubuh, sehingga udang akan mudah terserang penyakit. Tujuan dari penelitian ini adalah untuk mengetahui korelasi antara padat tebar dengan infestasi ektoparasit protozoa pada udang vaname (Litopenaeus vannamei) pada tambak super intensif. Penelitian ini merupakan penelitian Survei, dengan Rancangan Penelitian Cross Sectional Study, pengambilan sampel menggunakan metode proporsive sampling, yang dilakukan di daerah pertambakan di Kabupaten Tuban. Sampel udang yang diambil masing-masing sebanyak 50 ekor dari 3 petak tambak dengan padat tebar 150, 200 dan 300 ekor/m2. Hasil penelitian menunjukkan bahwa ektoparasit yang ditemukan pada udang vaname yang dipelihara pada tambak dengan padat tebar tinggi (super intensif) adalah Zoothamnium, Epistylis dan Vorticella, dengan intensitas berturut-turut sebesar 278,32 ; 391,34 dan 466,02 dengan derajat infestasi berat, pada semua udang yang dipelihara baik pada padat tebar 150, 200 dan 300 ekor/m2. Tidak ada korelasi antara padat tebar dengan infestasi ektoparasit pada udang vaname pada tambak super intensif, dengan nilai R = 0,394, adanya peningkatan padat tebar tidak diiringi dengan infestasi ketiga genus ektoparasit tersebut.

Blastocystis sp. : Evaluation of polyclonal antibody prepared from crude protein for serological diagnosis using Rabbit serum Septian Hakim Susantoputro; Lucia Tri Suwanti; Rahadju Ernawati; Mufasirin Mufasirin; Setiawan Koesdarto; Wiwiek Tyasningsih; Heni Puspitasari
Aceh Journal of Animal Science Vol 5, No 2 (2020): Desember 2020
Publisher : Syiah Kuala University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13170/ajas.5.2.16780

The diagnosis of Blastocystis infection is still based on the clinical sign which is not specific and there is no available serologic test for it. This study aimed to evaluate the polyclonal antibody prepared form crude protein of Blastocystis for the development of the Blastocystis serological test. Crude protein was extracted from the yeast of Blastocystis sp, then inoculated into rabbits to produce the antibody of crude protein. The serum of rabbits would be collected before and after immunization to compare the antibody titer. The profile of crude protein was analyzed using SDS-Page. The rabbit serum was analyzed using ELISA and Western Blot. The SDS-Page result showed bands in 100 kDa, 90 kDa, 70 kDa, 60 kDa, 58 kDa, 50 kDa, 40 kDa, 35 kDa, 30 kDa and 27 kDa. The ELISA assay showed that there was an increase in antibody titer of crude protein after immunization. Western Blot showed that three proteins (30 kDa, 40 kDa and 50 kDa) having immunogenicity characteristic. It is concluded that protein 30 kDa, 40 kDa and 50 kDa prepared from the crude protein of Blastocystis sp. can be used for developing a serologic test for Blastocystis infection. Keywords: Blastocystis sp, Crude Protein, Polyclonal Antibody .

The Prevalence of Gastrointestinal Tract Helminthiasis Through Stool Examination in Cattle at Benowo Landfill Surabaya Ratih Prajnya Paramitha; Rahaju Ernawati; Setiawan Koesdarto
Journal of Parasite Science (JoPS) Vol. 1 No. 1 (2017): Journal of Parasite Science
Publisher : Universitas Airlangga

Tujuan penelitian ini adalah untuk mengetahui prevalensi cacing, untuk mengetahui berbagai jenis telur cacing saluran cerna dan jenis umur terhadap infeksi cacing saluran cerna pada sapi di tanah Benowo, Surabaya. Sampel tinja diambil dari TPA sebanyak 41 sampel. Pemeriksaan tinja dilakukan dengan metode asli, sedimentasi sederhana dan pelapisan Fulleborn. Hasil penelitian menunjukkan bahwa 30 sampel positif menentang gastrointestinal, ini menunjukkan prevalensi cacing adalah 73%. Jenis telur cacing adalah kelas Nematode, yaitu Oesophagostomum sp., Trichostrongylus sp., Bunostomum sp. , Mecistocirrus digitatus, Trichuris sp dan Toxocara vitulorum.Menurut analisis statistik, dapat disimpulkan jenis dan jenis kelamin tidak dapat dihitung terhadap infeksi cacing.

Prevalence of Trypanosomiasis of Wild Rats (Rattus sp.) in Banyuwangi Zhaza Afililla; Lucia Tri Suwanti; Sri Agus Sudjarwo; Setiawan Koesdarto; Hani Plumeriastuti
Journal of Parasite Science (JoPS) Vol. 1 No. 2 (2017): Journal of Parasite Science
Publisher : Universitas Airlangga

The aim of this research was to investigate the number of prevalence of Trypanosomiasis of wild rats in Banyuwangi. Sixty wild rats were trapped from human residence, markets and rice fields in Banyuwangi. Rat`s Â blood smear was stained by Giemsa. The result show that one (1.67%.) of 60 blood sample was appear Trypanosoma sp.. Â The prevalence of Trypanosomiasis of wild rats in Banyuwangi was 1.67%.

Effect of Garlic (Allium sativum) Infusion On The Time of Death of Ascaridia galli Ida Yuniarti; Suzanita Utama; Setiawan Koesdarto; Lucia Tri Suwanti; Ngakan Made Rai Widjaja
Journal of Parasite Science (JoPS) Vol. 1 No. 2 (2017): Journal of Parasite Science
Publisher : Universitas Airlangga

Infectious disease is a major problem for animal husbandry. One of infectious disease is caused by Ascaridia galli (A. galli). Ascaridiasis occurs in either broiler or layer that caused decrease of meat and egg productions. Garlic (Allium sativum) contain alicinÂ that have the ability to kill parasites.This research was conducted to determine the effect of garlic on time of death of A. galli. In this research used A. galli with length 7-11 cm regardless the sex. The treatment used local (Indonesian garlic), kating and single garlic with concentration of 39.50%, 62.40% and 98.60%. The negative control used NaCl physiology (0.9 %). The result showed that all the treatment group can shortened the time of death of A. galli compared to control. The time of death of A. galli on local garlic infusion with 39.50 % concentration was 6.24 hours, on 62.40 % concentration was 4.94 hours and on 98.60 % concentration was 3.97 hours, kating garlic infusion on 39.50 % concentration was 5.08 hours on 62.40 % concentration was 3.81 hours, on 98.60 % concentration was 2.96 hours, single garlic infusion on 39.50 % concentration was 5.4 hours, on 62.40 % concentrationÂ was 3.86 hours, on 98.60 % concentration was 3.83 hours. The best treatment was kating garlic on 98.60% concentration because give shortest time of death.

Anthelmintic Activity of Basil Leaves (Ocimum sanctum Linn.) Infusion Against Ascaris suum In Vitro Ogen Sea; Mas'ud Hariadi; Setiawan Koesdarto; Kusnoto Kusnoto; Ngakan Made Rai Widjaja
Journal of Parasite Science (JoPS) Vol. 1 No. 2 (2017): Journal of Parasite Science
Publisher : Universitas Airlangga

Ascaris suum is a parasite nematode that causes infection in swines with high prevalence rates in host populations and usually associated with liver damages called â€œmilk spotsâ€ caused by larvae migration, resulting in organ condemnation. Basil leaves (Ocimum sanctum) phytochemical constituents contains flavonoid, phenol and tannin. Tannins and phenolics are known to interfere with the energy generation in helminth parasites by uncoupling oxidative phosphorylation and also bind to free proteins in the gastrointestinal tract of host animal or glycoprotein on the cuticle of the parasite and leading to death. This study was aimed to determine the activity of basil leaves (Ocimum sanctum) infusion in several concentrations against A. suum in vitro. This research used six treatments and four replications. This research used 10 A. suum in each treatment with four replication. The observations were done at 12, 18, 24, 30 and 36 hours in an incubator at 37oC. Based on the data analysis, basil leaves (Ocimum sanctum) infusion has anthelmintic activity against A. suum in vitro. The greater of the concentration and the longer of time of immersion, will make the death percentage of A. suum become higher. Concentration of basil leaves (Ocimum sanctum) infusion 15% is the effective concentration that can kill 100% of the A.suum during 36 hours of immersion.

The Anthelmintic Activity of Ethanol Extract of Basil Leaves (Ocimum americanum Linn.) Againt Haemonchus contortus Death in Vitro Rari Noviana; Chairul Anwar Nidom; Agus Sunarso; Setiawan Koesdarto; Sri Mumpuni Sosiawati; Iwan Sahrial Hamid
Journal of Parasite Science (JoPS) Vol. 1 No. 2 (2017): Journal of Parasite Science
Publisher : Universitas Airlangga

Tujuan dari penelitian ini adalah untuk mengetahui daya anthelmintik dari ekstrak etanol daun kemangi (Ocimum americanum Linn.) terhadap mortalitas cacing Haemonchus contortus secara in vitro. Sediaan ekstrak dibuat dengan konsentrasi (berat/volume) 2,5%, 5%, 7,5%, dan 10%. Untuk kontrol positif digunakan PBS dan DMSO sedangkan untuk kontrol negatif digunakan Albendazole 10 mg/ml. Sebanyak 10 ekor cacing Haemonchus contortus betina direndam dengan berbagai perlakuan dalam cawan petri. Masing-masing perlakuan diulang sebanyak 4 kali. Pengamatan dilakukan setiap 1 jam sekali selama 6 jam untuk melihat apakah cacing masih hidup atau sudah mati. Hasil dari penelitian ini menunjukkan bahwa ekstrak daun kemangi memiliki efek anthelmintik terhadap cacing Haemonchus contortus secara in vitro. Makin tinggi konsentrasi ekstrak, maka daya anthelmintiknya juga semakin tinggi. Pada ekstrak konsentrasi 10% memiliki kemampuan anthelmintik yang hampir sama dengan Albendazole.

The Prevalance and Helminth Infection Degree of Gastrointestinal in Layer Duck Located in Keper and Markolak Kramat Village District of Bangkalan Regency of Bangkalan Ana Amaliah; Indah Norma Triana; Poedji Hastutiek; Setiawan Koesdarto; Lucia Tri Suwanti; Soeharsono Soeharsono
Journal of Parasite Science (JoPS) Vol. 2 No. 1 (2018): Journal of Parasite Science
Publisher : Universitas Airlangga

Tujuan dari penelitian ini adalah untuk menentukan prevalensi dan derajat infeksi cacing saluran pencernaan pada itik petelur di dua kondisi lingkungan yang berbeda. Sampel feses diperiksa dengan metode sedimentasi dan metode apung kemudian dilanjutkan dengan menghitung telur cacing per gram tinja dengan metode Mc Master. Hasil menunjukkan bahwa 22,9% (8/35) itik petelur di Dusun Keper terinfeksi oleh Capillaria sp., dan Echinostoma revolutum, dengan rata-rata derajat infeksi sebsar 52,50 ± 41,662, sedangkan 31,4% (11/35) itik petelur di Dusun Markolak terinfeksi oleh Capillaria sp., Echinostoma revolutum, dan cacing dari kelas cestoda, dengan rata-rata derajat infeksi sebesar 155,45 ± 166,395. Infeksi bersifat tunggal maupun campuran. Analisis terhadap prevalensi dan derajat infeksi cacing saluran pencernaan itik petelur menunujukkan hasil yang tidak signifikan (p>0,05) antara Dusun Keper dan Dusun Markolak.

Co-Authors Adiacahya, Eren Adiana Mutamsari Witaningrum Agus Wijaya Akbar Haryo Widhi Trismiharto Aksono HP., Eduardus Bimo Aldi Hamdani Amelia Dwita Safitri Ana Amaliah Anita Nur Aida Aida Annisa Novia Damayanti APRIL HARI WARDHANA Arum Puspitasari Aryaloka, Suhita Ayuti, Siti Rani Azizatun Nimah Bagaskara, Ryan Bambang Sektiari Lukiswanto Boedi Setiawan Bryan Ahmad Affan Lubis BUDI UTOMO Budiarto Budiarto Budiarto Budiarto Dadik Rahardjo, Dadik Dewi Mariyam Dhimar Maulud Dyahningrum Dian Ayu Permatasari Didik Handijatno Didik Handijatno Djoko Poetranto E Djoko Poetranto Eka Pramyrtha Hestinah Emy Koestanti Sabdoningrum Endang Suprihati Eny Coolfina Simarmata Felita Widyaningsih Firman Hadi Fanani Galuh Swa Ardhya Gunanti Mahasri Hana Eliyani Hani Plumeriastuti Hasib, Abdullah Heni Puspitasari Hermin Ratnani Herry Agoes Hermadi Ida Yuniarti Indah Norma Triana, Indah Norma Indira Vania Carisya Inggarsetya Syah Audini Ira Sari Yudaniayanti Iwan Sahrial Hamid Iwan Sahrial Hamid Iwan Sahrial Hamid jayawardhana, andi Jessica Giovani Karina Rachmawati Khairullah, Aswin Rafif Khairunnisa, Hanifa Khansa Khalissa Farah Alifia Kismiyati , Koesnoto Supranianondo Koesnoto Supriandono Kuncoro Puguh Santoso Kuncorojakti, Suryo Kurnianto, Muhammad Aqil Kurniawati, Dyah Ayu Kusnoto Kusnoto Kusnoto Kusnoto, Kusnoto Lilik Maslachah Lucia Tri Suwanti, Lucia Tri Lutfiah Annisa Billa Mafruchati, Maslichah Mas’ud Hariadi Melani Anggraini Melanie Aulia Ashfiyah Meles, Dewa Ketut Mirni Lamid Mirny Lamid Mochamad Lazuardi Mohamad Safri Sauqi Moses, Ikechukwu Benjamin Muchammad Yunus Mufasirin Muhammad Rofi' Prasetya Muhammad Rofi' Prasetya Mustofa Helmi Effendi Nenny Harijani Ngakan Made Rai Widjaja Nidom, Chairul Anwar Nila Murodah Nisa’ Rachmaningtyas Putri Nove Hidajati Novia Intan Kurnia Nunuk Dyah Retno Lastuti Nunuk Dyah Retno Lastuti Nurhusien Yimer Ogen Sea Poedji Hastutiek Poetranto, Emmanuel Djoko Praja, Ratih Novita Prasetya, Muhammad Rofi' Pratiwi, Arif Primarizky, Hardany Pudji Srianto Putri, Valerie Brilianda Rahadju Ernawati Rahaju Ernawati Rahardjo, Adi Prijo Rahmi Sugihartuti Rari Noviana Ratih Prajnya Paramitha Rekasni Adallin A/P Morgan A/P Morgan Rimayanti Rimayanti Risky Aprillian, Risky Rochmah Kurniasanti Santosa, Gusti Ayu Illiyin Putri Sari, Aulia Puspita Sari, Putri Desi Wulan Sarmanu, Sarmanu Septian Hakim Susantoputro Sesa Puput Febriyanti Setiawati Sigit Soeharsono Soeharsono Soelih Estoepangestie, Agnes Theresia Sofie Adi, Keren Jayanti Sri Agus Sudjarwo Sri Mumpuni Sosiawati Sri Mumpuni Sosiawati Sunarso, Agus Suwarno Suwarno Suzanita Utama Syah Putra, Akyun Rozaqi Tanjung, Aldi Gusnizar Rizaldy Tita Damayanti Lestari Tjuk Imam Restiadi Trifena Pristi Anindyta Tyasningsih, Wiwiek Tyasningsih, Wiwik Vanna Lidya Kharisma Vindo Rossy Pertiwi Virgy Dzakina Fahrezy Warsito, Sunaryo Hadi Wibawati, Prima Ayu Widya Paramita Lokapirnasari Yeni Dhamayanti Yudhana, Aditya Yulianna Puspitasari Yunus, Muchammad Yunus, Muchammad Zhaza Afililla

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